Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome

Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, ne...
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Autor*in:	Yuguang Fu [verfasserIn] Marianne Wedde [verfasserIn] Sigrun Smola [verfasserIn] Djin-Ye Oh [verfasserIn] Thorsten Pfuhl [verfasserIn] Jürgen Rissland [verfasserIn] Michael Zemlin [verfasserIn] Fidelis A. Flockerzi [verfasserIn] Rainer M. Bohle [verfasserIn] Andrea Thürmer [verfasserIn] Susanne Duwe [verfasserIn] Barbara Biere [verfasserIn] Janine Reiche [verfasserIn] Brunhilde Schweiger [verfasserIn] Christin Mache [verfasserIn] Thorsten Wolff [verfasserIn] Georg Herrler [verfasserIn] Ralf Dürrwald [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	A(H1N1)pdm09 virus Fatal influenza S263S/F (HA1) and H456H/Y (PB1) mutations

Übergeordnetes Werk:	In: International Journal of Medical Microbiology - Elsevier, 2021, 314(2024), Seite 151598-
Übergeordnetes Werk:	volume:314 ; year:2024 ; pages:151598-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.ijmm.2024.151598

Katalog-ID:	DOAJ097495662

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allfields_unstemmed	10.1016/j.ijmm.2024.151598 doi (DE-627)DOAJ097495662 (DE-599)DOAJf28aedf87c504504a2ae355ef5707073 DE-627 ger DE-627 rakwb eng QR1-502 RZ201-999 Yuguang Fu verfasserin aut Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome. A(H1N1)pdm09 virus Fatal influenza S263S/F (HA1) and H456H/Y (PB1) mutations Microbiology Other systems of medicine Marianne Wedde verfasserin aut Sigrun Smola verfasserin aut Djin-Ye Oh verfasserin aut Thorsten Pfuhl verfasserin aut Jürgen Rissland verfasserin aut Michael Zemlin verfasserin aut Fidelis A. Flockerzi verfasserin aut Rainer M. Bohle verfasserin aut Andrea Thürmer verfasserin aut Susanne Duwe verfasserin aut Barbara Biere verfasserin aut Janine Reiche verfasserin aut Brunhilde Schweiger verfasserin aut Christin Mache verfasserin aut Thorsten Wolff verfasserin aut Georg Herrler verfasserin aut Ralf Dürrwald verfasserin aut In International Journal of Medical Microbiology Elsevier, 2021 314(2024), Seite 151598- (DE-627)320603938 (DE-600)2020515-6 16180607 nnns volume:314 year:2024 pages:151598- https://doi.org/10.1016/j.ijmm.2024.151598 kostenfrei https://doaj.org/article/f28aedf87c504504a2ae355ef5707073 kostenfrei http://www.sciencedirect.com/science/article/pii/S143842212400002X kostenfrei https://doaj.org/toc/1438-4221 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 314 2024 151598-
allfieldsGer	10.1016/j.ijmm.2024.151598 doi (DE-627)DOAJ097495662 (DE-599)DOAJf28aedf87c504504a2ae355ef5707073 DE-627 ger DE-627 rakwb eng QR1-502 RZ201-999 Yuguang Fu verfasserin aut Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome. A(H1N1)pdm09 virus Fatal influenza S263S/F (HA1) and H456H/Y (PB1) mutations Microbiology Other systems of medicine Marianne Wedde verfasserin aut Sigrun Smola verfasserin aut Djin-Ye Oh verfasserin aut Thorsten Pfuhl verfasserin aut Jürgen Rissland verfasserin aut Michael Zemlin verfasserin aut Fidelis A. Flockerzi verfasserin aut Rainer M. Bohle verfasserin aut Andrea Thürmer verfasserin aut Susanne Duwe verfasserin aut Barbara Biere verfasserin aut Janine Reiche verfasserin aut Brunhilde Schweiger verfasserin aut Christin Mache verfasserin aut Thorsten Wolff verfasserin aut Georg Herrler verfasserin aut Ralf Dürrwald verfasserin aut In International Journal of Medical Microbiology Elsevier, 2021 314(2024), Seite 151598- (DE-627)320603938 (DE-600)2020515-6 16180607 nnns volume:314 year:2024 pages:151598- https://doi.org/10.1016/j.ijmm.2024.151598 kostenfrei https://doaj.org/article/f28aedf87c504504a2ae355ef5707073 kostenfrei http://www.sciencedirect.com/science/article/pii/S143842212400002X kostenfrei https://doaj.org/toc/1438-4221 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 314 2024 151598-
allfieldsSound	10.1016/j.ijmm.2024.151598 doi (DE-627)DOAJ097495662 (DE-599)DOAJf28aedf87c504504a2ae355ef5707073 DE-627 ger DE-627 rakwb eng QR1-502 RZ201-999 Yuguang Fu verfasserin aut Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome. A(H1N1)pdm09 virus Fatal influenza S263S/F (HA1) and H456H/Y (PB1) mutations Microbiology Other systems of medicine Marianne Wedde verfasserin aut Sigrun Smola verfasserin aut Djin-Ye Oh verfasserin aut Thorsten Pfuhl verfasserin aut Jürgen Rissland verfasserin aut Michael Zemlin verfasserin aut Fidelis A. Flockerzi verfasserin aut Rainer M. Bohle verfasserin aut Andrea Thürmer verfasserin aut Susanne Duwe verfasserin aut Barbara Biere verfasserin aut Janine Reiche verfasserin aut Brunhilde Schweiger verfasserin aut Christin Mache verfasserin aut Thorsten Wolff verfasserin aut Georg Herrler verfasserin aut Ralf Dürrwald verfasserin aut In International Journal of Medical Microbiology Elsevier, 2021 314(2024), Seite 151598- (DE-627)320603938 (DE-600)2020515-6 16180607 nnns volume:314 year:2024 pages:151598- https://doi.org/10.1016/j.ijmm.2024.151598 kostenfrei https://doaj.org/article/f28aedf87c504504a2ae355ef5707073 kostenfrei http://www.sciencedirect.com/science/article/pii/S143842212400002X kostenfrei https://doaj.org/toc/1438-4221 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 314 2024 151598-
language	English
source	In International Journal of Medical Microbiology 314(2024), Seite 151598- volume:314 year:2024 pages:151598-
sourceStr	In International Journal of Medical Microbiology 314(2024), Seite 151598- volume:314 year:2024 pages:151598-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	A(H1N1)pdm09 virus Fatal influenza S263S/F (HA1) and H456H/Y (PB1) mutations Microbiology Other systems of medicine
isfreeaccess_bool	true
container_title	International Journal of Medical Microbiology
authorswithroles_txt_mv	Yuguang Fu @@aut@@ Marianne Wedde @@aut@@ Sigrun Smola @@aut@@ Djin-Ye Oh @@aut@@ Thorsten Pfuhl @@aut@@ Jürgen Rissland @@aut@@ Michael Zemlin @@aut@@ Fidelis A. Flockerzi @@aut@@ Rainer M. Bohle @@aut@@ Andrea Thürmer @@aut@@ Susanne Duwe @@aut@@ Barbara Biere @@aut@@ Janine Reiche @@aut@@ Brunhilde Schweiger @@aut@@ Christin Mache @@aut@@ Thorsten Wolff @@aut@@ Georg Herrler @@aut@@ Ralf Dürrwald @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	320603938
id	DOAJ097495662
language_de	englisch
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callnumber-first	Q - Science
author	Yuguang Fu
spellingShingle	Yuguang Fu misc QR1-502 misc RZ201-999 misc A(H1N1)pdm09 virus misc Fatal influenza misc S263S/F (HA1) and H456H/Y (PB1) mutations misc Microbiology misc Other systems of medicine Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome
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topic_title	QR1-502 RZ201-999 Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome A(H1N1)pdm09 virus Fatal influenza S263S/F (HA1) and H456H/Y (PB1) mutations
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title	Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome
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title_full	Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome
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author_browse	Yuguang Fu Marianne Wedde Sigrun Smola Djin-Ye Oh Thorsten Pfuhl Jürgen Rissland Michael Zemlin Fidelis A. Flockerzi Rainer M. Bohle Andrea Thürmer Susanne Duwe Barbara Biere Janine Reiche Brunhilde Schweiger Christin Mache Thorsten Wolff Georg Herrler Ralf Dürrwald
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title_sort	different populations of a(h1n1)pdm09 viruses in a patient with hemolytic-uremic syndrome
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title_auth	Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome
abstract	Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
abstractGer	Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
abstract_unstemmed	Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized.A patient (age group 5–15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined.Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed.The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
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title_short	Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome
url	https://doi.org/10.1016/j.ijmm.2024.151598 https://doaj.org/article/f28aedf87c504504a2ae355ef5707073 http://www.sciencedirect.com/science/article/pii/S143842212400002X https://doaj.org/toc/1438-4221
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author2Str	Marianne Wedde Sigrun Smola Djin-Ye Oh Thorsten Pfuhl Jürgen Rissland Michael Zemlin Fidelis A. Flockerzi Rainer M. Bohle Andrea Thürmer Susanne Duwe Barbara Biere Janine Reiche Brunhilde Schweiger Christin Mache Thorsten Wolff Georg Herrler Ralf Dürrwald
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Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome

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