An optimized protocol for isolation of murine pancreatic single cells with high yield and purity

Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields o...
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Autor*in:	Feijing Wu [verfasserIn] Zhengyu Jiang [verfasserIn] Jin Qian [verfasserIn] Hiroki Kobayashi [verfasserIn] Quin T. Waterbury [verfasserIn] Ruth A. White [verfasserIn] Yosuke Ochiai [verfasserIn] Xiaofei Zhi [verfasserIn] Ruhong Tu [verfasserIn] Biyun Zheng [verfasserIn] Qiongyu Shi [verfasserIn] Leah B. Zamechek [verfasserIn] Timothy C. Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Cell Biology Cell isolation Single Cell Flow Cytometry Organoids

Übergeordnetes Werk:	In: STAR Protocols - Elsevier, 2020, 5(2024), 1, Seite 102836-
Übergeordnetes Werk:	volume:5 ; year:2024 ; number:1 ; pages:102836-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.xpro.2024.102836

Katalog-ID:	DOAJ097638668

Internformat


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520			\|a Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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650		4	\|a Cell isolation
650		4	\|a Single Cell
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700	0		\|a Biyun Zheng \|e verfasserin \|4 aut
700	0		\|a Qiongyu Shi \|e verfasserin \|4 aut
700	0		\|a Leah B. Zamechek \|e verfasserin \|4 aut
700	0		\|a Timothy C. Wang \|e verfasserin \|4 aut
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912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
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912			\|a GBV_ILN_2010
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912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
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912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
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912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2112
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912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
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allfields	10.1016/j.xpro.2024.102836 doi (DE-627)DOAJ097638668 (DE-599)DOAJc3193e334731495c8e251f2e78edfb50 DE-627 ger DE-627 rakwb eng Q1-390 Feijing Wu verfasserin aut An optimized protocol for isolation of murine pancreatic single cells with high yield and purity 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell Biology Cell isolation Single Cell Flow Cytometry Organoids Science (General) Zhengyu Jiang verfasserin aut Jin Qian verfasserin aut Hiroki Kobayashi verfasserin aut Quin T. Waterbury verfasserin aut Ruth A. White verfasserin aut Yosuke Ochiai verfasserin aut Xiaofei Zhi verfasserin aut Ruhong Tu verfasserin aut Biyun Zheng verfasserin aut Qiongyu Shi verfasserin aut Leah B. Zamechek verfasserin aut Timothy C. Wang verfasserin aut In STAR Protocols Elsevier, 2020 5(2024), 1, Seite 102836- (DE-627)1747970557 26661667 nnns volume:5 year:2024 number:1 pages:102836- https://doi.org/10.1016/j.xpro.2024.102836 kostenfrei https://doaj.org/article/c3193e334731495c8e251f2e78edfb50 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166724000017 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 5 2024 1 102836-
spelling	10.1016/j.xpro.2024.102836 doi (DE-627)DOAJ097638668 (DE-599)DOAJc3193e334731495c8e251f2e78edfb50 DE-627 ger DE-627 rakwb eng Q1-390 Feijing Wu verfasserin aut An optimized protocol for isolation of murine pancreatic single cells with high yield and purity 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell Biology Cell isolation Single Cell Flow Cytometry Organoids Science (General) Zhengyu Jiang verfasserin aut Jin Qian verfasserin aut Hiroki Kobayashi verfasserin aut Quin T. Waterbury verfasserin aut Ruth A. White verfasserin aut Yosuke Ochiai verfasserin aut Xiaofei Zhi verfasserin aut Ruhong Tu verfasserin aut Biyun Zheng verfasserin aut Qiongyu Shi verfasserin aut Leah B. Zamechek verfasserin aut Timothy C. Wang verfasserin aut In STAR Protocols Elsevier, 2020 5(2024), 1, Seite 102836- (DE-627)1747970557 26661667 nnns volume:5 year:2024 number:1 pages:102836- https://doi.org/10.1016/j.xpro.2024.102836 kostenfrei https://doaj.org/article/c3193e334731495c8e251f2e78edfb50 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166724000017 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 5 2024 1 102836-
allfields_unstemmed	10.1016/j.xpro.2024.102836 doi (DE-627)DOAJ097638668 (DE-599)DOAJc3193e334731495c8e251f2e78edfb50 DE-627 ger DE-627 rakwb eng Q1-390 Feijing Wu verfasserin aut An optimized protocol for isolation of murine pancreatic single cells with high yield and purity 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell Biology Cell isolation Single Cell Flow Cytometry Organoids Science (General) Zhengyu Jiang verfasserin aut Jin Qian verfasserin aut Hiroki Kobayashi verfasserin aut Quin T. Waterbury verfasserin aut Ruth A. White verfasserin aut Yosuke Ochiai verfasserin aut Xiaofei Zhi verfasserin aut Ruhong Tu verfasserin aut Biyun Zheng verfasserin aut Qiongyu Shi verfasserin aut Leah B. Zamechek verfasserin aut Timothy C. Wang verfasserin aut In STAR Protocols Elsevier, 2020 5(2024), 1, Seite 102836- (DE-627)1747970557 26661667 nnns volume:5 year:2024 number:1 pages:102836- https://doi.org/10.1016/j.xpro.2024.102836 kostenfrei https://doaj.org/article/c3193e334731495c8e251f2e78edfb50 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166724000017 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 5 2024 1 102836-
allfieldsGer	10.1016/j.xpro.2024.102836 doi (DE-627)DOAJ097638668 (DE-599)DOAJc3193e334731495c8e251f2e78edfb50 DE-627 ger DE-627 rakwb eng Q1-390 Feijing Wu verfasserin aut An optimized protocol for isolation of murine pancreatic single cells with high yield and purity 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell Biology Cell isolation Single Cell Flow Cytometry Organoids Science (General) Zhengyu Jiang verfasserin aut Jin Qian verfasserin aut Hiroki Kobayashi verfasserin aut Quin T. Waterbury verfasserin aut Ruth A. White verfasserin aut Yosuke Ochiai verfasserin aut Xiaofei Zhi verfasserin aut Ruhong Tu verfasserin aut Biyun Zheng verfasserin aut Qiongyu Shi verfasserin aut Leah B. Zamechek verfasserin aut Timothy C. Wang verfasserin aut In STAR Protocols Elsevier, 2020 5(2024), 1, Seite 102836- (DE-627)1747970557 26661667 nnns volume:5 year:2024 number:1 pages:102836- https://doi.org/10.1016/j.xpro.2024.102836 kostenfrei https://doaj.org/article/c3193e334731495c8e251f2e78edfb50 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166724000017 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 5 2024 1 102836-
allfieldsSound	10.1016/j.xpro.2024.102836 doi (DE-627)DOAJ097638668 (DE-599)DOAJc3193e334731495c8e251f2e78edfb50 DE-627 ger DE-627 rakwb eng Q1-390 Feijing Wu verfasserin aut An optimized protocol for isolation of murine pancreatic single cells with high yield and purity 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell Biology Cell isolation Single Cell Flow Cytometry Organoids Science (General) Zhengyu Jiang verfasserin aut Jin Qian verfasserin aut Hiroki Kobayashi verfasserin aut Quin T. Waterbury verfasserin aut Ruth A. White verfasserin aut Yosuke Ochiai verfasserin aut Xiaofei Zhi verfasserin aut Ruhong Tu verfasserin aut Biyun Zheng verfasserin aut Qiongyu Shi verfasserin aut Leah B. Zamechek verfasserin aut Timothy C. Wang verfasserin aut In STAR Protocols Elsevier, 2020 5(2024), 1, Seite 102836- (DE-627)1747970557 26661667 nnns volume:5 year:2024 number:1 pages:102836- https://doi.org/10.1016/j.xpro.2024.102836 kostenfrei https://doaj.org/article/c3193e334731495c8e251f2e78edfb50 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166724000017 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 5 2024 1 102836-
language	English
source	In STAR Protocols 5(2024), 1, Seite 102836- volume:5 year:2024 number:1 pages:102836-
sourceStr	In STAR Protocols 5(2024), 1, Seite 102836- volume:5 year:2024 number:1 pages:102836-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Cell Biology Cell isolation Single Cell Flow Cytometry Organoids Science (General)
isfreeaccess_bool	true
container_title	STAR Protocols
authorswithroles_txt_mv	Feijing Wu @@aut@@ Zhengyu Jiang @@aut@@ Jin Qian @@aut@@ Hiroki Kobayashi @@aut@@ Quin T. Waterbury @@aut@@ Ruth A. White @@aut@@ Yosuke Ochiai @@aut@@ Xiaofei Zhi @@aut@@ Ruhong Tu @@aut@@ Biyun Zheng @@aut@@ Qiongyu Shi @@aut@@ Leah B. Zamechek @@aut@@ Timothy C. Wang @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	1747970557
id	DOAJ097638668
language_de	englisch
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callnumber-first	Q - Science
author	Feijing Wu
spellingShingle	Feijing Wu misc Q1-390 misc Cell Biology misc Cell isolation misc Single Cell misc Flow Cytometry misc Organoids misc Science (General) An optimized protocol for isolation of murine pancreatic single cells with high yield and purity
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topic_title	Q1-390 An optimized protocol for isolation of murine pancreatic single cells with high yield and purity Cell Biology Cell isolation Single Cell Flow Cytometry Organoids
topic	misc Q1-390 misc Cell Biology misc Cell isolation misc Single Cell misc Flow Cytometry misc Organoids misc Science (General)
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title	An optimized protocol for isolation of murine pancreatic single cells with high yield and purity
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title_full	An optimized protocol for isolation of murine pancreatic single cells with high yield and purity
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author_browse	Feijing Wu Zhengyu Jiang Jin Qian Hiroki Kobayashi Quin T. Waterbury Ruth A. White Yosuke Ochiai Xiaofei Zhi Ruhong Tu Biyun Zheng Qiongyu Shi Leah B. Zamechek Timothy C. Wang
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title_sort	optimized protocol for isolation of murine pancreatic single cells with high yield and purity
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title_auth	An optimized protocol for isolation of murine pancreatic single cells with high yield and purity
abstract	Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
abstractGer	Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
abstract_unstemmed	Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields of viable pancreatic single cells. This protocol can be applied across a wide range of research areas, including single-cell sequencing, gene expression profiling, primary cell culture, and even the development of spheroids or organoids.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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title_short	An optimized protocol for isolation of murine pancreatic single cells with high yield and purity
url	https://doi.org/10.1016/j.xpro.2024.102836 https://doaj.org/article/c3193e334731495c8e251f2e78edfb50 http://www.sciencedirect.com/science/article/pii/S2666166724000017 https://doaj.org/toc/2666-1667
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An optimized protocol for isolation of murine pancreatic single cells with high yield and purity

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