Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics

GABA<sub<A</sub< receptors are the major inhibitory receptors in the brain. They are hetero-pentamers with a composition of predominantly two α, two β, and one γ or δ subunit. Of the six α subunit genes, the α5 subunit displays a limited spatial expression pattern and is known to mediate...
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Autor*in:	Miao Chen [verfasserIn] Frank Koopmans [verfasserIn] Miguel A. Gonzalez-Lozano [verfasserIn] August B. Smit [verfasserIn] Ka Wan Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	GABA immunoprecipitation proteomics brain synapse immunocytochemistry

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 13(2023), 1, p 14
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:1, p 14

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells13010014

Katalog-ID:	DOAJ097807907

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institution	findex.gbv.de
topic_facet	GABA<sub<A</sub< receptor immunoprecipitation proteomics brain synapse immunocytochemistry Cytology
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container_title	Cells
authorswithroles_txt_mv	Miao Chen @@aut@@ Frank Koopmans @@aut@@ Miguel A. Gonzalez-Lozano @@aut@@ August B. Smit @@aut@@ Ka Wan Li @@aut@@
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callnumber-first	Q - Science
author	Miao Chen
spellingShingle	Miao Chen misc QH573-671 misc GABA<sub<A</sub< receptor misc immunoprecipitation misc proteomics misc brain misc synapse misc immunocytochemistry misc Cytology Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics
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topic_title	QH573-671 Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics GABA<sub<A</sub< receptor immunoprecipitation proteomics brain synapse immunocytochemistry
topic	misc QH573-671 misc GABA<sub<A</sub< receptor misc immunoprecipitation misc proteomics misc brain misc synapse misc immunocytochemistry misc Cytology
topic_unstemmed	misc QH573-671 misc GABA<sub<A</sub< receptor misc immunoprecipitation misc proteomics misc brain misc synapse misc immunocytochemistry misc Cytology
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title	Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics
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title_full	Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics
author_sort	Miao Chen
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author_browse	Miao Chen Frank Koopmans Miguel A. Gonzalez-Lozano August B. Smit Ka Wan Li
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title_sort	brain region differences in α1- and α5-subunit-containing gaba<sub<a</sub< receptor proteomes revealed with affinity purification and blue native page proteomics
callnumber	QH573-671
title_auth	Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics
abstract	GABA<sub<A</sub< receptors are the major inhibitory receptors in the brain. They are hetero-pentamers with a composition of predominantly two α, two β, and one γ or δ subunit. Of the six α subunit genes, the α5 subunit displays a limited spatial expression pattern and is known to mediate both phasic and tonic inhibition. In this study, using immunoaffinity-based proteomics, we identified the α5 subunit containing receptor complexes in the hippocampus and olfactory bulb. The α1–α5 interaction was identified in both brain regions, albeit with significantly different stoichiometries. In line with this, reverse IPs using anti-α1 antibodies showed the α5–α1 co-occurrence and validated the quantitative difference. In addition, we showed that the association of Neuroligin 2 with α1-containing receptors was much higher in the olfactory bulb than in the hippocampus, which was confirmed using blue native gel electrophoresis and quantitative mass spectrometry. Finally, immunocytochemical staining revealed a co-localization of α1 and α5 subunits in the post-synaptic puncta in the hippocampus.
abstractGer	GABA<sub<A</sub< receptors are the major inhibitory receptors in the brain. They are hetero-pentamers with a composition of predominantly two α, two β, and one γ or δ subunit. Of the six α subunit genes, the α5 subunit displays a limited spatial expression pattern and is known to mediate both phasic and tonic inhibition. In this study, using immunoaffinity-based proteomics, we identified the α5 subunit containing receptor complexes in the hippocampus and olfactory bulb. The α1–α5 interaction was identified in both brain regions, albeit with significantly different stoichiometries. In line with this, reverse IPs using anti-α1 antibodies showed the α5–α1 co-occurrence and validated the quantitative difference. In addition, we showed that the association of Neuroligin 2 with α1-containing receptors was much higher in the olfactory bulb than in the hippocampus, which was confirmed using blue native gel electrophoresis and quantitative mass spectrometry. Finally, immunocytochemical staining revealed a co-localization of α1 and α5 subunits in the post-synaptic puncta in the hippocampus.
abstract_unstemmed	GABA<sub<A</sub< receptors are the major inhibitory receptors in the brain. They are hetero-pentamers with a composition of predominantly two α, two β, and one γ or δ subunit. Of the six α subunit genes, the α5 subunit displays a limited spatial expression pattern and is known to mediate both phasic and tonic inhibition. In this study, using immunoaffinity-based proteomics, we identified the α5 subunit containing receptor complexes in the hippocampus and olfactory bulb. The α1–α5 interaction was identified in both brain regions, albeit with significantly different stoichiometries. In line with this, reverse IPs using anti-α1 antibodies showed the α5–α1 co-occurrence and validated the quantitative difference. In addition, we showed that the association of Neuroligin 2 with α1-containing receptors was much higher in the olfactory bulb than in the hippocampus, which was confirmed using blue native gel electrophoresis and quantitative mass spectrometry. Finally, immunocytochemical staining revealed a co-localization of α1 and α5 subunits in the post-synaptic puncta in the hippocampus.
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title_short	Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics
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Brain Region Differences in α1- and α5-Subunit-Containing GABA<sub<A</sub< Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics

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