The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions
BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lisa Pagan [verfasserIn] Bertine W. Huisman [verfasserIn] Michelle van der Wurff [verfasserIn] Rosanne G. C. Naafs [verfasserIn] Frank H. J. Schuren [verfasserIn] Ingrid M. J. G. Sanders [verfasserIn] Wiep Klaas Smits [verfasserIn] Romy D. Zwittink [verfasserIn] Jacobus Burggraaf [verfasserIn] Robert Rissmann [verfasserIn] Jurgen M. J. Piek [verfasserIn] Jannie G. E. Henderickx [verfasserIn] Mariëtte I. E. van Poelgeest [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 14(2023) |
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| Übergeordnetes Werk: |
volume:14 ; year:2023 |
| Links: |
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| DOI / URN: |
10.3389/fmicb.2023.1264768 |
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| Katalog-ID: |
DOAJ097882895 |
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| 245 | 1 | 4 | |a The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions |
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| 520 | |a BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. | ||
| 650 | 4 | |a vulvar microbiome | |
| 650 | 4 | |a vaginal microbiome | |
| 650 | 4 | |a lichen sclerosus | |
| 650 | 4 | |a vulvar HSIL | |
| 650 | 4 | |a HPV | |
| 650 | 4 | |a vulvar cancer | |
| 653 | 0 | |a Microbiology | |
| 700 | 0 | |a Lisa Pagan |e verfasserin |4 aut | |
| 700 | 0 | |a Bertine W. Huisman |e verfasserin |4 aut | |
| 700 | 0 | |a Bertine W. Huisman |e verfasserin |4 aut | |
| 700 | 0 | |a Michelle van der Wurff |e verfasserin |4 aut | |
| 700 | 0 | |a Rosanne G. C. Naafs |e verfasserin |4 aut | |
| 700 | 0 | |a Frank H. J. Schuren |e verfasserin |4 aut | |
| 700 | 0 | |a Ingrid M. J. G. Sanders |e verfasserin |4 aut | |
| 700 | 0 | |a Wiep Klaas Smits |e verfasserin |4 aut | |
| 700 | 0 | |a Wiep Klaas Smits |e verfasserin |4 aut | |
| 700 | 0 | |a Romy D. Zwittink |e verfasserin |4 aut | |
| 700 | 0 | |a Romy D. Zwittink |e verfasserin |4 aut | |
| 700 | 0 | |a Jacobus Burggraaf |e verfasserin |4 aut | |
| 700 | 0 | |a Jacobus Burggraaf |e verfasserin |4 aut | |
| 700 | 0 | |a Robert Rissmann |e verfasserin |4 aut | |
| 700 | 0 | |a Robert Rissmann |e verfasserin |4 aut | |
| 700 | 0 | |a Robert Rissmann |e verfasserin |4 aut | |
| 700 | 0 | |a Jurgen M. J. Piek |e verfasserin |4 aut | |
| 700 | 0 | |a Jannie G. E. Henderickx |e verfasserin |4 aut | |
| 700 | 0 | |a Jannie G. E. Henderickx |e verfasserin |4 aut | |
| 700 | 0 | |a Mariëtte I. E. van Poelgeest |e verfasserin |4 aut | |
| 700 | 0 | |a Mariëtte I. E. van Poelgeest |e verfasserin |4 aut | |
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10.3389/fmicb.2023.1264768 doi (DE-627)DOAJ097882895 (DE-599)DOAJ2b7bddac6cb54f87b3c2c8152822db06 DE-627 ger DE-627 rakwb eng QR1-502 Lisa Pagan verfasserin aut The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. vulvar microbiome vaginal microbiome lichen sclerosus vulvar HSIL HPV vulvar cancer Microbiology Lisa Pagan verfasserin aut Bertine W. Huisman verfasserin aut Bertine W. Huisman verfasserin aut Michelle van der Wurff verfasserin aut Rosanne G. C. Naafs verfasserin aut Frank H. J. Schuren verfasserin aut Ingrid M. J. G. Sanders verfasserin aut Wiep Klaas Smits verfasserin aut Wiep Klaas Smits verfasserin aut Romy D. Zwittink verfasserin aut Romy D. Zwittink verfasserin aut Jacobus Burggraaf verfasserin aut Jacobus Burggraaf verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Jurgen M. J. Piek verfasserin aut Jannie G. E. Henderickx verfasserin aut Jannie G. E. Henderickx verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 14(2023) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:14 year:2023 https://doi.org/10.3389/fmicb.2023.1264768 kostenfrei https://doaj.org/article/2b7bddac6cb54f87b3c2c8152822db06 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264768/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 |
| spelling |
10.3389/fmicb.2023.1264768 doi (DE-627)DOAJ097882895 (DE-599)DOAJ2b7bddac6cb54f87b3c2c8152822db06 DE-627 ger DE-627 rakwb eng QR1-502 Lisa Pagan verfasserin aut The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. vulvar microbiome vaginal microbiome lichen sclerosus vulvar HSIL HPV vulvar cancer Microbiology Lisa Pagan verfasserin aut Bertine W. Huisman verfasserin aut Bertine W. Huisman verfasserin aut Michelle van der Wurff verfasserin aut Rosanne G. C. Naafs verfasserin aut Frank H. J. Schuren verfasserin aut Ingrid M. J. G. Sanders verfasserin aut Wiep Klaas Smits verfasserin aut Wiep Klaas Smits verfasserin aut Romy D. Zwittink verfasserin aut Romy D. Zwittink verfasserin aut Jacobus Burggraaf verfasserin aut Jacobus Burggraaf verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Jurgen M. J. Piek verfasserin aut Jannie G. E. Henderickx verfasserin aut Jannie G. E. Henderickx verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 14(2023) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:14 year:2023 https://doi.org/10.3389/fmicb.2023.1264768 kostenfrei https://doaj.org/article/2b7bddac6cb54f87b3c2c8152822db06 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264768/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 |
| allfields_unstemmed |
10.3389/fmicb.2023.1264768 doi (DE-627)DOAJ097882895 (DE-599)DOAJ2b7bddac6cb54f87b3c2c8152822db06 DE-627 ger DE-627 rakwb eng QR1-502 Lisa Pagan verfasserin aut The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. vulvar microbiome vaginal microbiome lichen sclerosus vulvar HSIL HPV vulvar cancer Microbiology Lisa Pagan verfasserin aut Bertine W. Huisman verfasserin aut Bertine W. Huisman verfasserin aut Michelle van der Wurff verfasserin aut Rosanne G. C. Naafs verfasserin aut Frank H. J. Schuren verfasserin aut Ingrid M. J. G. Sanders verfasserin aut Wiep Klaas Smits verfasserin aut Wiep Klaas Smits verfasserin aut Romy D. Zwittink verfasserin aut Romy D. Zwittink verfasserin aut Jacobus Burggraaf verfasserin aut Jacobus Burggraaf verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Jurgen M. J. Piek verfasserin aut Jannie G. E. Henderickx verfasserin aut Jannie G. E. Henderickx verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 14(2023) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:14 year:2023 https://doi.org/10.3389/fmicb.2023.1264768 kostenfrei https://doaj.org/article/2b7bddac6cb54f87b3c2c8152822db06 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264768/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 |
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10.3389/fmicb.2023.1264768 doi (DE-627)DOAJ097882895 (DE-599)DOAJ2b7bddac6cb54f87b3c2c8152822db06 DE-627 ger DE-627 rakwb eng QR1-502 Lisa Pagan verfasserin aut The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. vulvar microbiome vaginal microbiome lichen sclerosus vulvar HSIL HPV vulvar cancer Microbiology Lisa Pagan verfasserin aut Bertine W. Huisman verfasserin aut Bertine W. Huisman verfasserin aut Michelle van der Wurff verfasserin aut Rosanne G. C. Naafs verfasserin aut Frank H. J. Schuren verfasserin aut Ingrid M. J. G. Sanders verfasserin aut Wiep Klaas Smits verfasserin aut Wiep Klaas Smits verfasserin aut Romy D. Zwittink verfasserin aut Romy D. Zwittink verfasserin aut Jacobus Burggraaf verfasserin aut Jacobus Burggraaf verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Jurgen M. J. Piek verfasserin aut Jannie G. E. Henderickx verfasserin aut Jannie G. E. Henderickx verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 14(2023) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:14 year:2023 https://doi.org/10.3389/fmicb.2023.1264768 kostenfrei https://doaj.org/article/2b7bddac6cb54f87b3c2c8152822db06 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264768/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 |
| allfieldsSound |
10.3389/fmicb.2023.1264768 doi (DE-627)DOAJ097882895 (DE-599)DOAJ2b7bddac6cb54f87b3c2c8152822db06 DE-627 ger DE-627 rakwb eng QR1-502 Lisa Pagan verfasserin aut The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. vulvar microbiome vaginal microbiome lichen sclerosus vulvar HSIL HPV vulvar cancer Microbiology Lisa Pagan verfasserin aut Bertine W. Huisman verfasserin aut Bertine W. Huisman verfasserin aut Michelle van der Wurff verfasserin aut Rosanne G. C. Naafs verfasserin aut Frank H. J. Schuren verfasserin aut Ingrid M. J. G. Sanders verfasserin aut Wiep Klaas Smits verfasserin aut Wiep Klaas Smits verfasserin aut Romy D. Zwittink verfasserin aut Romy D. Zwittink verfasserin aut Jacobus Burggraaf verfasserin aut Jacobus Burggraaf verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Robert Rissmann verfasserin aut Jurgen M. J. Piek verfasserin aut Jannie G. E. Henderickx verfasserin aut Jannie G. E. Henderickx verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut Mariëtte I. E. van Poelgeest verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 14(2023) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:14 year:2023 https://doi.org/10.3389/fmicb.2023.1264768 kostenfrei https://doaj.org/article/2b7bddac6cb54f87b3c2c8152822db06 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264768/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 |
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Lisa Pagan @@aut@@ Bertine W. Huisman @@aut@@ Michelle van der Wurff @@aut@@ Rosanne G. C. Naafs @@aut@@ Frank H. J. Schuren @@aut@@ Ingrid M. J. G. Sanders @@aut@@ Wiep Klaas Smits @@aut@@ Romy D. Zwittink @@aut@@ Jacobus Burggraaf @@aut@@ Robert Rissmann @@aut@@ Jurgen M. J. Piek @@aut@@ Jannie G. E. Henderickx @@aut@@ Mariëtte I. E. van Poelgeest @@aut@@ |
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The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. 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Lisa Pagan Bertine W. Huisman Michelle van der Wurff Rosanne G. C. Naafs Frank H. J. Schuren Ingrid M. J. G. Sanders Wiep Klaas Smits Romy D. Zwittink Jacobus Burggraaf Robert Rissmann Jurgen M. J. Piek Jannie G. E. Henderickx Mariëtte I. E. van Poelgeest |
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vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions |
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The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions |
| abstract |
BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. |
| abstractGer |
BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. |
| abstract_unstemmed |
BackgroundThe role of the vulvar microbiome in the development of (pre)malignant vulvar disease is scarcely investigated. The aim of this exploratory study was to analyze vulvar microbiome composition in lichen sclerosus (LS) and vulvar high-grade squamous intraepithelial lesions (HSIL) compared to healthy controls.MethodsWomen with vulvar lichen sclerosus (n = 10), HSIL (n = 5) and healthy controls (n = 10) were included. Swabs were collected from the vulva, vagina and anal region for microbiome characterization by metagenomic shotgun sequencing. Both lesional and non-lesional sites were examined. Biophysical assessments included trans-epidermal water loss for evaluation of the vulvar skin barrier function and vulvar and vaginal pH measurements.ResultsHealthy vulvar skin resembled vaginal, anal and skin-like microbiome composition, including the genera Prevotella, Lactobacillus, Gardnerella, Staphylococcus, Cutibacterium, and Corynebacterium. Significant differences were observed in diversity between vulvar skin of healthy controls and LS patients. Compared to the healthy vulvar skin, vulvar microbiome composition of both LS and vulvar HSIL patients was characterized by significantly higher proportions of, respectively, Papillomaviridae (p = 0.045) and Alphapapillomavirus (p = 0.002). In contrast, the Prevotella genus (p = 0.031) and Bacteroidales orders (p = 0.038) were significantly less abundant in LS, as was the Actinobacteria class (p = 0.040) in vulvar HSIL. While bacteria and viruses were most abundant, fungal and archaeal taxa were scarcely observed. Trans-epidermal water loss was higher in vulvar HSIL compared to healthy vulvar skin (p = 0.043).ConclusionThis study is the first to examine the vulvar microbiome through metagenomic shotgun sequencing in LS and HSIL patients. Diseased vulvar skin presents a distinct signature compared to healthy vulvar skin with respect to bacterial and viral fractions of the microbiome. Key findings include the presence of papillomaviruses in LS as well as in vulvar HSIL, although LS is generally considered an HPV-independent risk factor for vulvar dysplasia. This exploratory study provides clues to the etiology of vulvar premalignancies and may act as a steppingstone for expanding the knowledge on potential drivers of disease progression. |
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The vulvar microbiome in lichen sclerosus and high-grade intraepithelial lesions |
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Lisa Pagan Bertine W. Huisman Michelle van der Wurff Rosanne G. C. Naafs Frank H. J. Schuren Ingrid M. J. G. Sanders Wiep Klaas Smits Romy D. Zwittink Jacobus Burggraaf Robert Rissmann Jurgen M. J. Piek Jannie G. E. Henderickx Mariëtte I. E. van Poelgeest |
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