Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes
Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-b...
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| Autor*in: |
SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Chinesisch |
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2023 |
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In: Jichu yixue yu linchuang ; 43(2023), 10, Seite 1542-1548 volume:43 ; year:2023 ; number:10 ; pages:1542-1548 |
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| Links: |
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| DOI / URN: |
10.16352/j.issn.1001-6325.2023.10.1542 |
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| Katalog-ID: |
DOAJ097902772 |
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| 520 | |a Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. | ||
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10.16352/j.issn.1001-6325.2023.10.1542 doi (DE-627)DOAJ097902772 (DE-599)DOAJ6b566a32e1934059a2f1b3e5f7412fad DE-627 ger DE-627 rakwb chi SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao verfasserin aut Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. tim-3|galectin-9|t helper cells|jak2/stat3 pathway|sepsis Medicine R In Jichu yixue yu linchuang 43(2023), 10, Seite 1542-1548 volume:43 year:2023 number:10 pages:1542-1548 https://doi.org/10.16352/j.issn.1001-6325.2023.10.1542 kostenfrei https://doaj.org/article/6b566a32e1934059a2f1b3e5f7412fad kostenfrei http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1542.pdf kostenfrei https://doaj.org/toc/1001-6325 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2023 10 1542-1548 |
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10.16352/j.issn.1001-6325.2023.10.1542 doi (DE-627)DOAJ097902772 (DE-599)DOAJ6b566a32e1934059a2f1b3e5f7412fad DE-627 ger DE-627 rakwb chi SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao verfasserin aut Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. tim-3|galectin-9|t helper cells|jak2/stat3 pathway|sepsis Medicine R In Jichu yixue yu linchuang 43(2023), 10, Seite 1542-1548 volume:43 year:2023 number:10 pages:1542-1548 https://doi.org/10.16352/j.issn.1001-6325.2023.10.1542 kostenfrei https://doaj.org/article/6b566a32e1934059a2f1b3e5f7412fad kostenfrei http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1542.pdf kostenfrei https://doaj.org/toc/1001-6325 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2023 10 1542-1548 |
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10.16352/j.issn.1001-6325.2023.10.1542 doi (DE-627)DOAJ097902772 (DE-599)DOAJ6b566a32e1934059a2f1b3e5f7412fad DE-627 ger DE-627 rakwb chi SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao verfasserin aut Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. tim-3|galectin-9|t helper cells|jak2/stat3 pathway|sepsis Medicine R In Jichu yixue yu linchuang 43(2023), 10, Seite 1542-1548 volume:43 year:2023 number:10 pages:1542-1548 https://doi.org/10.16352/j.issn.1001-6325.2023.10.1542 kostenfrei https://doaj.org/article/6b566a32e1934059a2f1b3e5f7412fad kostenfrei http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1542.pdf kostenfrei https://doaj.org/toc/1001-6325 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2023 10 1542-1548 |
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10.16352/j.issn.1001-6325.2023.10.1542 doi (DE-627)DOAJ097902772 (DE-599)DOAJ6b566a32e1934059a2f1b3e5f7412fad DE-627 ger DE-627 rakwb chi SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao verfasserin aut Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. tim-3|galectin-9|t helper cells|jak2/stat3 pathway|sepsis Medicine R In Jichu yixue yu linchuang 43(2023), 10, Seite 1542-1548 volume:43 year:2023 number:10 pages:1542-1548 https://doi.org/10.16352/j.issn.1001-6325.2023.10.1542 kostenfrei https://doaj.org/article/6b566a32e1934059a2f1b3e5f7412fad kostenfrei http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1542.pdf kostenfrei https://doaj.org/toc/1001-6325 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2023 10 1542-1548 |
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10.16352/j.issn.1001-6325.2023.10.1542 doi (DE-627)DOAJ097902772 (DE-599)DOAJ6b566a32e1934059a2f1b3e5f7412fad DE-627 ger DE-627 rakwb chi SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao verfasserin aut Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. tim-3|galectin-9|t helper cells|jak2/stat3 pathway|sepsis Medicine R In Jichu yixue yu linchuang 43(2023), 10, Seite 1542-1548 volume:43 year:2023 number:10 pages:1542-1548 https://doi.org/10.16352/j.issn.1001-6325.2023.10.1542 kostenfrei https://doaj.org/article/6b566a32e1934059a2f1b3e5f7412fad kostenfrei http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1542.pdf kostenfrei https://doaj.org/toc/1001-6325 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 43 2023 10 1542-1548 |
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Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes tim-3|galectin-9|t helper cells|jak2/stat3 pathway|sepsis |
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misc tim-3|galectin-9|t helper cells|jak2/stat3 pathway|sepsis misc Medicine misc R |
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Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes |
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Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes |
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SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao |
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Jichu yixue yu linchuang |
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2023 |
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SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao |
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43 |
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Elektronische Aufsätze |
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SUN Xiaoli, ZHANG Jinjin, SONG Fengying, HUANG Yongli, CHEN Lili, XING Yanchao |
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10.16352/j.issn.1001-6325.2023.10.1542 |
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tim-3/galectin-9 regulates th1/th2 cell balance in lipopolysaccharide-induced human lymphocytes |
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Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes |
| abstract |
Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. |
| abstractGer |
Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. |
| abstract_unstemmed |
Objective To investigate the mechanism of Tim-3/galectin-9 in T helper type 1/2 (Th1/Th2) cell imbalance in lipopolysaccharide(LPS)-induced human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from patients with sepsis. The expression of Tim-3, galectin-9, T-bet and GATA3 were detected by RT-qPCR; ELISA was used for detecting sTim-3, galectin-9, IFN-γ and IL-4.Isolated peripheral blood mononuclear cells were incubated with LPS and then cytokines related to Th1 and Th2 cells in the supernatant of cell culture were detected by ELISA; Western blot was used to detect the expression of JAK2/STAT3 related factors; Pearson correlation coefficient analysis was performed to identify relationship among Tim-3, galectin-9,IFN-γ and IL-4. Results The mRNA expression of Tim-3 and galectin-9 in peripheral blood of patients with sepsis was increased, and protein expression of Tim-3, galectin-9, IL-4 and GATA-3 was increased significantly. The levels of IFN-γ and T-bet were decreased and levels of p-JAK2 and p-STAT3 were elevated. After blocking Tim-3 expression, IFN-γ level increased, while IL-4 level decreased significantly. Phosphorylation of JAK2 and STAT3 decreased. Level of Tim-3 and of IL-4 was positively correlated with galectin-9, while Tim-3 was negatively correlated with IFN-γ;IFN-γ was negatively correlated with galectin-9, and Tim-3 was positively correlated with IL-4. Conclusions Tim-3/galectin-9 regulates the JAK2/STAT3 pathway and leads to the imbalance of Th1/Th2 cells in LPS induced inflammation. |
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Tim-3/galectin-9 regulates Th1/Th2 cell balance in lipopolysaccharide-induced human lymphocytes |
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https://doi.org/10.16352/j.issn.1001-6325.2023.10.1542 https://doaj.org/article/6b566a32e1934059a2f1b3e5f7412fad http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1542.pdf https://doaj.org/toc/1001-6325 |
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