Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma

PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we a...
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Autor*in:	Duo Wang [verfasserIn] Liuying Huang [verfasserIn] Danqi Qian [verfasserIn] Yulin Cao [verfasserIn] Xiaohan Wu [verfasserIn] Peiwen Xu [verfasserIn] Liang Ming [verfasserIn] Junhui Tang [verfasserIn] Zhaohui Huang [verfasserIn] Yuan Yin [verfasserIn] Leyuan Zhou [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	lung cancer low dose radiotherapy tertiary lymphoid structures immunotherapy tumor microenvironment

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 14(2024)
Übergeordnetes Werk:	volume:14 ; year:2024

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2023.1334408

Katalog-ID:	DOAJ097939706

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520			\|a PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect.
650		4	\|a lung cancer
650		4	\|a low dose radiotherapy
650		4	\|a tertiary lymphoid structures
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650		4	\|a tumor microenvironment
653		0	\|a Immunologic diseases. Allergy
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allfields	10.3389/fimmu.2023.1334408 doi (DE-627)DOAJ097939706 (DE-599)DOAJcf9a95f3b19a479db8e5132ead26435c DE-627 ger DE-627 rakwb eng RC581-607 Duo Wang verfasserin aut Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect. lung cancer low dose radiotherapy tertiary lymphoid structures immunotherapy tumor microenvironment Immunologic diseases. Allergy Duo Wang verfasserin aut Liuying Huang verfasserin aut Liuying Huang verfasserin aut Danqi Qian verfasserin aut Yulin Cao verfasserin aut Xiaohan Wu verfasserin aut Peiwen Xu verfasserin aut Liang Ming verfasserin aut Junhui Tang verfasserin aut Zhaohui Huang verfasserin aut Yuan Yin verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2024) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2024 https://doi.org/10.3389/fimmu.2023.1334408 kostenfrei https://doaj.org/article/cf9a95f3b19a479db8e5132ead26435c kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1334408/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024
spelling	10.3389/fimmu.2023.1334408 doi (DE-627)DOAJ097939706 (DE-599)DOAJcf9a95f3b19a479db8e5132ead26435c DE-627 ger DE-627 rakwb eng RC581-607 Duo Wang verfasserin aut Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect. lung cancer low dose radiotherapy tertiary lymphoid structures immunotherapy tumor microenvironment Immunologic diseases. Allergy Duo Wang verfasserin aut Liuying Huang verfasserin aut Liuying Huang verfasserin aut Danqi Qian verfasserin aut Yulin Cao verfasserin aut Xiaohan Wu verfasserin aut Peiwen Xu verfasserin aut Liang Ming verfasserin aut Junhui Tang verfasserin aut Zhaohui Huang verfasserin aut Yuan Yin verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2024) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2024 https://doi.org/10.3389/fimmu.2023.1334408 kostenfrei https://doaj.org/article/cf9a95f3b19a479db8e5132ead26435c kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1334408/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024
allfields_unstemmed	10.3389/fimmu.2023.1334408 doi (DE-627)DOAJ097939706 (DE-599)DOAJcf9a95f3b19a479db8e5132ead26435c DE-627 ger DE-627 rakwb eng RC581-607 Duo Wang verfasserin aut Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect. lung cancer low dose radiotherapy tertiary lymphoid structures immunotherapy tumor microenvironment Immunologic diseases. Allergy Duo Wang verfasserin aut Liuying Huang verfasserin aut Liuying Huang verfasserin aut Danqi Qian verfasserin aut Yulin Cao verfasserin aut Xiaohan Wu verfasserin aut Peiwen Xu verfasserin aut Liang Ming verfasserin aut Junhui Tang verfasserin aut Zhaohui Huang verfasserin aut Yuan Yin verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2024) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2024 https://doi.org/10.3389/fimmu.2023.1334408 kostenfrei https://doaj.org/article/cf9a95f3b19a479db8e5132ead26435c kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1334408/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024
allfieldsGer	10.3389/fimmu.2023.1334408 doi (DE-627)DOAJ097939706 (DE-599)DOAJcf9a95f3b19a479db8e5132ead26435c DE-627 ger DE-627 rakwb eng RC581-607 Duo Wang verfasserin aut Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect. lung cancer low dose radiotherapy tertiary lymphoid structures immunotherapy tumor microenvironment Immunologic diseases. Allergy Duo Wang verfasserin aut Liuying Huang verfasserin aut Liuying Huang verfasserin aut Danqi Qian verfasserin aut Yulin Cao verfasserin aut Xiaohan Wu verfasserin aut Peiwen Xu verfasserin aut Liang Ming verfasserin aut Junhui Tang verfasserin aut Zhaohui Huang verfasserin aut Yuan Yin verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2024) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2024 https://doi.org/10.3389/fimmu.2023.1334408 kostenfrei https://doaj.org/article/cf9a95f3b19a479db8e5132ead26435c kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1334408/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024
allfieldsSound	10.3389/fimmu.2023.1334408 doi (DE-627)DOAJ097939706 (DE-599)DOAJcf9a95f3b19a479db8e5132ead26435c DE-627 ger DE-627 rakwb eng RC581-607 Duo Wang verfasserin aut Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect. lung cancer low dose radiotherapy tertiary lymphoid structures immunotherapy tumor microenvironment Immunologic diseases. Allergy Duo Wang verfasserin aut Liuying Huang verfasserin aut Liuying Huang verfasserin aut Danqi Qian verfasserin aut Yulin Cao verfasserin aut Xiaohan Wu verfasserin aut Peiwen Xu verfasserin aut Liang Ming verfasserin aut Junhui Tang verfasserin aut Zhaohui Huang verfasserin aut Yuan Yin verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut Leyuan Zhou verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 14(2024) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:14 year:2024 https://doi.org/10.3389/fimmu.2023.1334408 kostenfrei https://doaj.org/article/cf9a95f3b19a479db8e5132ead26435c kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2023.1334408/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024
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However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. 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callnumber-first	R - Medicine
author	Duo Wang
spellingShingle	Duo Wang misc RC581-607 misc lung cancer misc low dose radiotherapy misc tertiary lymphoid structures misc immunotherapy misc tumor microenvironment misc Immunologic diseases. Allergy Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma
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topic_title	RC581-607 Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma lung cancer low dose radiotherapy tertiary lymphoid structures immunotherapy tumor microenvironment
topic	misc RC581-607 misc lung cancer misc low dose radiotherapy misc tertiary lymphoid structures misc immunotherapy misc tumor microenvironment misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc lung cancer misc low dose radiotherapy misc tertiary lymphoid structures misc immunotherapy misc tumor microenvironment misc Immunologic diseases. Allergy
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title	Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma
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title_full	Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma
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author_browse	Duo Wang Liuying Huang Danqi Qian Yulin Cao Xiaohan Wu Peiwen Xu Liang Ming Junhui Tang Zhaohui Huang Yuan Yin Leyuan Zhou
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title_sort	low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma
callnumber	RC581-607
title_auth	Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma
abstract	PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect.
abstractGer	PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect.
abstract_unstemmed	PurposeA tertiary lymphoid structure (TLS) refers to an organized infiltration of immune cells that is linked to a positive prognosis and improved response to immunotherapy. However, methods that promote TLS formation are limited and challenging to implement in clinical settings. In this study, we aimed to promote the formation and maturation of TLSs in lung adenocarcinoma (LUAD) by combining low-dose radiotherapy (LDRT) with immunotherapy.MethodsTissue sections from 198 patients who had undergone surgery were examined. Risk factors for patient survival were assessed, and the relationship between TLSs and five-year survival was analyzed. The Kras-LSL-G12D spontaneous lung cancer mouse model was used to screen the optimal irradiation dose (0/1/2 Gy whole lung irradiation) for promoting TLS formation. LDRT combined with anti-PD-1 was used to promote the formation and maturation of TLSs.ResultsTLS+, TLSHigh, TLS+GC+ and CD8High within TLS+ were associated with a favorable prognosis. LDRT increased the formation of early TLSs in the Kras-LSL-G12D lung cancer mouse model. In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. This approach achieved a promising antitumor effect.
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title_short	Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma
url	https://doi.org/10.3389/fimmu.2023.1334408 https://doaj.org/article/cf9a95f3b19a479db8e5132ead26435c https://www.frontiersin.org/articles/10.3389/fimmu.2023.1334408/full https://doaj.org/toc/1664-3224
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author2	Duo Wang Liuying Huang Danqi Qian Yulin Cao Xiaohan Wu Peiwen Xu Liang Ming Junhui Tang Zhaohui Huang Yuan Yin Leyuan Zhou
author2Str	Duo Wang Liuying Huang Danqi Qian Yulin Cao Xiaohan Wu Peiwen Xu Liang Ming Junhui Tang Zhaohui Huang Yuan Yin Leyuan Zhou
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up_date	2024-07-03T14:33:58.969Z
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In addition, LDRT combined with anti-PD-1 treatment can significantly improve the maturity of TLSs in mouse LUAD, resulting in greater antitumor effects. This antitumor effect was strongly associated with the number of CD8+ T cells within the TLSs.ConclusionWe successfully applied LDRT combined with PD-1 inhibitor therapy for the first time, which increased both the quantity and maturity of TLSs in lung cancer. 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Low-dose radiotherapy promotes the formation of tertiary lymphoid structures in lung adenocarcinoma

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