MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing
Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and spe...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Han Xia [verfasserIn] Zhe Zhang [verfasserIn] Chen Luo [verfasserIn] Kangfei Wei [verfasserIn] Xuming Li [verfasserIn] Xiyu Mu [verfasserIn] Meilin Duan [verfasserIn] Chuanlong Zhu [verfasserIn] Luyi Jin [verfasserIn] Xiaoqing He [verfasserIn] Lingjie Tang [verfasserIn] Long Hu [verfasserIn] Yuanlin Guan [verfasserIn] David C. C. Lam [verfasserIn] Junbo Yang [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: iMeta - Wiley, 2022, 2(2023), 4, Seite n/a-n/a |
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| Übergeordnetes Werk: |
volume:2 ; year:2023 ; number:4 ; pages:n/a-n/a |
| Links: |
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| DOI / URN: |
10.1002/imt2.143 |
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| Katalog-ID: |
DOAJ09804642X |
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| 520 | |a Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. | ||
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| 650 | 4 | |a minimal primer set | |
| 650 | 4 | |a multiplex PCR | |
| 650 | 4 | |a targeted next‐generation sequencing | |
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| 700 | 0 | |a Zhe Zhang |e verfasserin |4 aut | |
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| 700 | 0 | |a Kangfei Wei |e verfasserin |4 aut | |
| 700 | 0 | |a Xuming Li |e verfasserin |4 aut | |
| 700 | 0 | |a Xiyu Mu |e verfasserin |4 aut | |
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10.1002/imt2.143 doi (DE-627)DOAJ09804642X (DE-599)DOAJ1de3649de6a5492eb1a75a4f402796c0 DE-627 ger DE-627 rakwb eng R858-859.7 Han Xia verfasserin aut MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. degenerate primer design minimal primer set multiplex PCR targeted next‐generation sequencing Computer applications to medicine. Medical informatics Zhe Zhang verfasserin aut Chen Luo verfasserin aut Kangfei Wei verfasserin aut Xuming Li verfasserin aut Xiyu Mu verfasserin aut Meilin Duan verfasserin aut Chuanlong Zhu verfasserin aut Luyi Jin verfasserin aut Xiaoqing He verfasserin aut Lingjie Tang verfasserin aut Long Hu verfasserin aut Yuanlin Guan verfasserin aut David C. C. Lam verfasserin aut Junbo Yang verfasserin aut In iMeta Wiley, 2022 2(2023), 4, Seite n/a-n/a (DE-627)1797101269 (DE-600)3114873-6 2770596X nnns volume:2 year:2023 number:4 pages:n/a-n/a https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/article/1de3649de6a5492eb1a75a4f402796c0 kostenfrei https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/toc/2770-596X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2023 4 n/a-n/a |
| spelling |
10.1002/imt2.143 doi (DE-627)DOAJ09804642X (DE-599)DOAJ1de3649de6a5492eb1a75a4f402796c0 DE-627 ger DE-627 rakwb eng R858-859.7 Han Xia verfasserin aut MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. degenerate primer design minimal primer set multiplex PCR targeted next‐generation sequencing Computer applications to medicine. Medical informatics Zhe Zhang verfasserin aut Chen Luo verfasserin aut Kangfei Wei verfasserin aut Xuming Li verfasserin aut Xiyu Mu verfasserin aut Meilin Duan verfasserin aut Chuanlong Zhu verfasserin aut Luyi Jin verfasserin aut Xiaoqing He verfasserin aut Lingjie Tang verfasserin aut Long Hu verfasserin aut Yuanlin Guan verfasserin aut David C. C. Lam verfasserin aut Junbo Yang verfasserin aut In iMeta Wiley, 2022 2(2023), 4, Seite n/a-n/a (DE-627)1797101269 (DE-600)3114873-6 2770596X nnns volume:2 year:2023 number:4 pages:n/a-n/a https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/article/1de3649de6a5492eb1a75a4f402796c0 kostenfrei https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/toc/2770-596X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2023 4 n/a-n/a |
| allfields_unstemmed |
10.1002/imt2.143 doi (DE-627)DOAJ09804642X (DE-599)DOAJ1de3649de6a5492eb1a75a4f402796c0 DE-627 ger DE-627 rakwb eng R858-859.7 Han Xia verfasserin aut MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. degenerate primer design minimal primer set multiplex PCR targeted next‐generation sequencing Computer applications to medicine. Medical informatics Zhe Zhang verfasserin aut Chen Luo verfasserin aut Kangfei Wei verfasserin aut Xuming Li verfasserin aut Xiyu Mu verfasserin aut Meilin Duan verfasserin aut Chuanlong Zhu verfasserin aut Luyi Jin verfasserin aut Xiaoqing He verfasserin aut Lingjie Tang verfasserin aut Long Hu verfasserin aut Yuanlin Guan verfasserin aut David C. C. Lam verfasserin aut Junbo Yang verfasserin aut In iMeta Wiley, 2022 2(2023), 4, Seite n/a-n/a (DE-627)1797101269 (DE-600)3114873-6 2770596X nnns volume:2 year:2023 number:4 pages:n/a-n/a https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/article/1de3649de6a5492eb1a75a4f402796c0 kostenfrei https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/toc/2770-596X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2023 4 n/a-n/a |
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10.1002/imt2.143 doi (DE-627)DOAJ09804642X (DE-599)DOAJ1de3649de6a5492eb1a75a4f402796c0 DE-627 ger DE-627 rakwb eng R858-859.7 Han Xia verfasserin aut MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. degenerate primer design minimal primer set multiplex PCR targeted next‐generation sequencing Computer applications to medicine. Medical informatics Zhe Zhang verfasserin aut Chen Luo verfasserin aut Kangfei Wei verfasserin aut Xuming Li verfasserin aut Xiyu Mu verfasserin aut Meilin Duan verfasserin aut Chuanlong Zhu verfasserin aut Luyi Jin verfasserin aut Xiaoqing He verfasserin aut Lingjie Tang verfasserin aut Long Hu verfasserin aut Yuanlin Guan verfasserin aut David C. C. Lam verfasserin aut Junbo Yang verfasserin aut In iMeta Wiley, 2022 2(2023), 4, Seite n/a-n/a (DE-627)1797101269 (DE-600)3114873-6 2770596X nnns volume:2 year:2023 number:4 pages:n/a-n/a https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/article/1de3649de6a5492eb1a75a4f402796c0 kostenfrei https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/toc/2770-596X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2023 4 n/a-n/a |
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10.1002/imt2.143 doi (DE-627)DOAJ09804642X (DE-599)DOAJ1de3649de6a5492eb1a75a4f402796c0 DE-627 ger DE-627 rakwb eng R858-859.7 Han Xia verfasserin aut MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. degenerate primer design minimal primer set multiplex PCR targeted next‐generation sequencing Computer applications to medicine. Medical informatics Zhe Zhang verfasserin aut Chen Luo verfasserin aut Kangfei Wei verfasserin aut Xuming Li verfasserin aut Xiyu Mu verfasserin aut Meilin Duan verfasserin aut Chuanlong Zhu verfasserin aut Luyi Jin verfasserin aut Xiaoqing He verfasserin aut Lingjie Tang verfasserin aut Long Hu verfasserin aut Yuanlin Guan verfasserin aut David C. C. Lam verfasserin aut Junbo Yang verfasserin aut In iMeta Wiley, 2022 2(2023), 4, Seite n/a-n/a (DE-627)1797101269 (DE-600)3114873-6 2770596X nnns volume:2 year:2023 number:4 pages:n/a-n/a https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/article/1de3649de6a5492eb1a75a4f402796c0 kostenfrei https://doi.org/10.1002/imt2.143 kostenfrei https://doaj.org/toc/2770-596X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2023 4 n/a-n/a |
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Han Xia @@aut@@ Zhe Zhang @@aut@@ Chen Luo @@aut@@ Kangfei Wei @@aut@@ Xuming Li @@aut@@ Xiyu Mu @@aut@@ Meilin Duan @@aut@@ Chuanlong Zhu @@aut@@ Luyi Jin @@aut@@ Xiaoqing He @@aut@@ Lingjie Tang @@aut@@ Long Hu @@aut@@ Yuanlin Guan @@aut@@ David C. C. Lam @@aut@@ Junbo Yang @@aut@@ |
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MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing |
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Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. |
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Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. |
| abstract_unstemmed |
Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%. |
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