An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments
Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Motonari Okabe [verfasserIn] Hiromitsu Shirasawa [verfasserIn] Yuki Ono [verfasserIn] Mayumi Goto [verfasserIn] Takuya Iwasawa [verfasserIn] Taichi Sakaguchi [verfasserIn] Akiko Fujishima [verfasserIn] Yohei Onodera [verfasserIn] Kenichi Makino [verfasserIn] Hiroshi Miura [verfasserIn] Yukiyo Kumazawa [verfasserIn] Kazumasa Takahashi [verfasserIn] Yukihiro Terada [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Reproductive Medicine and Biology - Wiley, 2017, 22(2023), 1, Seite n/a-n/a |
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| Übergeordnetes Werk: |
volume:22 ; year:2023 ; number:1 ; pages:n/a-n/a |
| Links: |
Link aufrufen |
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| DOI / URN: |
10.1002/rmb2.12551 |
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| Katalog-ID: |
DOAJ098132237 |
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| 245 | 1 | 3 | |a An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments |
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| 520 | |a Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. | ||
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| 650 | 4 | |a embryonic development | |
| 650 | 4 | |a fluorescent probes | |
| 650 | 4 | |a live cell imaging | |
| 650 | 4 | |a two‐pronuclear stage | |
| 653 | 0 | |a Diseases of the endocrine glands. Clinical endocrinology | |
| 653 | 0 | |a Reproduction | |
| 700 | 0 | |a Hiromitsu Shirasawa |e verfasserin |4 aut | |
| 700 | 0 | |a Yuki Ono |e verfasserin |4 aut | |
| 700 | 0 | |a Mayumi Goto |e verfasserin |4 aut | |
| 700 | 0 | |a Takuya Iwasawa |e verfasserin |4 aut | |
| 700 | 0 | |a Taichi Sakaguchi |e verfasserin |4 aut | |
| 700 | 0 | |a Akiko Fujishima |e verfasserin |4 aut | |
| 700 | 0 | |a Yohei Onodera |e verfasserin |4 aut | |
| 700 | 0 | |a Kenichi Makino |e verfasserin |4 aut | |
| 700 | 0 | |a Hiroshi Miura |e verfasserin |4 aut | |
| 700 | 0 | |a Yukiyo Kumazawa |e verfasserin |4 aut | |
| 700 | 0 | |a Kazumasa Takahashi |e verfasserin |4 aut | |
| 700 | 0 | |a Yukihiro Terada |e verfasserin |4 aut | |
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10.1002/rmb2.12551 doi (DE-627)DOAJ098132237 (DE-599)DOAJ8bac129827a24c33a475bb8c7a2d975b DE-627 ger DE-627 rakwb eng RC648-665 QH471-489 Motonari Okabe verfasserin aut An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. confocal microscope embryonic development fluorescent probes live cell imaging two‐pronuclear stage Diseases of the endocrine glands. Clinical endocrinology Reproduction Hiromitsu Shirasawa verfasserin aut Yuki Ono verfasserin aut Mayumi Goto verfasserin aut Takuya Iwasawa verfasserin aut Taichi Sakaguchi verfasserin aut Akiko Fujishima verfasserin aut Yohei Onodera verfasserin aut Kenichi Makino verfasserin aut Hiroshi Miura verfasserin aut Yukiyo Kumazawa verfasserin aut Kazumasa Takahashi verfasserin aut Yukihiro Terada verfasserin aut In Reproductive Medicine and Biology Wiley, 2017 22(2023), 1, Seite n/a-n/a (DE-627)349876223 (DE-600)2081579-7 14470578 nnns volume:22 year:2023 number:1 pages:n/a-n/a https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/article/8bac129827a24c33a475bb8c7a2d975b kostenfrei https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/toc/1445-5781 Journal toc kostenfrei https://doaj.org/toc/1447-0578 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2023 1 n/a-n/a |
| spelling |
10.1002/rmb2.12551 doi (DE-627)DOAJ098132237 (DE-599)DOAJ8bac129827a24c33a475bb8c7a2d975b DE-627 ger DE-627 rakwb eng RC648-665 QH471-489 Motonari Okabe verfasserin aut An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. confocal microscope embryonic development fluorescent probes live cell imaging two‐pronuclear stage Diseases of the endocrine glands. Clinical endocrinology Reproduction Hiromitsu Shirasawa verfasserin aut Yuki Ono verfasserin aut Mayumi Goto verfasserin aut Takuya Iwasawa verfasserin aut Taichi Sakaguchi verfasserin aut Akiko Fujishima verfasserin aut Yohei Onodera verfasserin aut Kenichi Makino verfasserin aut Hiroshi Miura verfasserin aut Yukiyo Kumazawa verfasserin aut Kazumasa Takahashi verfasserin aut Yukihiro Terada verfasserin aut In Reproductive Medicine and Biology Wiley, 2017 22(2023), 1, Seite n/a-n/a (DE-627)349876223 (DE-600)2081579-7 14470578 nnns volume:22 year:2023 number:1 pages:n/a-n/a https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/article/8bac129827a24c33a475bb8c7a2d975b kostenfrei https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/toc/1445-5781 Journal toc kostenfrei https://doaj.org/toc/1447-0578 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2023 1 n/a-n/a |
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10.1002/rmb2.12551 doi (DE-627)DOAJ098132237 (DE-599)DOAJ8bac129827a24c33a475bb8c7a2d975b DE-627 ger DE-627 rakwb eng RC648-665 QH471-489 Motonari Okabe verfasserin aut An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. confocal microscope embryonic development fluorescent probes live cell imaging two‐pronuclear stage Diseases of the endocrine glands. Clinical endocrinology Reproduction Hiromitsu Shirasawa verfasserin aut Yuki Ono verfasserin aut Mayumi Goto verfasserin aut Takuya Iwasawa verfasserin aut Taichi Sakaguchi verfasserin aut Akiko Fujishima verfasserin aut Yohei Onodera verfasserin aut Kenichi Makino verfasserin aut Hiroshi Miura verfasserin aut Yukiyo Kumazawa verfasserin aut Kazumasa Takahashi verfasserin aut Yukihiro Terada verfasserin aut In Reproductive Medicine and Biology Wiley, 2017 22(2023), 1, Seite n/a-n/a (DE-627)349876223 (DE-600)2081579-7 14470578 nnns volume:22 year:2023 number:1 pages:n/a-n/a https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/article/8bac129827a24c33a475bb8c7a2d975b kostenfrei https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/toc/1445-5781 Journal toc kostenfrei https://doaj.org/toc/1447-0578 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2023 1 n/a-n/a |
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10.1002/rmb2.12551 doi (DE-627)DOAJ098132237 (DE-599)DOAJ8bac129827a24c33a475bb8c7a2d975b DE-627 ger DE-627 rakwb eng RC648-665 QH471-489 Motonari Okabe verfasserin aut An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. confocal microscope embryonic development fluorescent probes live cell imaging two‐pronuclear stage Diseases of the endocrine glands. Clinical endocrinology Reproduction Hiromitsu Shirasawa verfasserin aut Yuki Ono verfasserin aut Mayumi Goto verfasserin aut Takuya Iwasawa verfasserin aut Taichi Sakaguchi verfasserin aut Akiko Fujishima verfasserin aut Yohei Onodera verfasserin aut Kenichi Makino verfasserin aut Hiroshi Miura verfasserin aut Yukiyo Kumazawa verfasserin aut Kazumasa Takahashi verfasserin aut Yukihiro Terada verfasserin aut In Reproductive Medicine and Biology Wiley, 2017 22(2023), 1, Seite n/a-n/a (DE-627)349876223 (DE-600)2081579-7 14470578 nnns volume:22 year:2023 number:1 pages:n/a-n/a https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/article/8bac129827a24c33a475bb8c7a2d975b kostenfrei https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/toc/1445-5781 Journal toc kostenfrei https://doaj.org/toc/1447-0578 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2023 1 n/a-n/a |
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10.1002/rmb2.12551 doi (DE-627)DOAJ098132237 (DE-599)DOAJ8bac129827a24c33a475bb8c7a2d975b DE-627 ger DE-627 rakwb eng RC648-665 QH471-489 Motonari Okabe verfasserin aut An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. confocal microscope embryonic development fluorescent probes live cell imaging two‐pronuclear stage Diseases of the endocrine glands. Clinical endocrinology Reproduction Hiromitsu Shirasawa verfasserin aut Yuki Ono verfasserin aut Mayumi Goto verfasserin aut Takuya Iwasawa verfasserin aut Taichi Sakaguchi verfasserin aut Akiko Fujishima verfasserin aut Yohei Onodera verfasserin aut Kenichi Makino verfasserin aut Hiroshi Miura verfasserin aut Yukiyo Kumazawa verfasserin aut Kazumasa Takahashi verfasserin aut Yukihiro Terada verfasserin aut In Reproductive Medicine and Biology Wiley, 2017 22(2023), 1, Seite n/a-n/a (DE-627)349876223 (DE-600)2081579-7 14470578 nnns volume:22 year:2023 number:1 pages:n/a-n/a https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/article/8bac129827a24c33a475bb8c7a2d975b kostenfrei https://doi.org/10.1002/rmb2.12551 kostenfrei https://doaj.org/toc/1445-5781 Journal toc kostenfrei https://doaj.org/toc/1447-0578 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2023 1 n/a-n/a |
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Motonari Okabe @@aut@@ Hiromitsu Shirasawa @@aut@@ Yuki Ono @@aut@@ Mayumi Goto @@aut@@ Takuya Iwasawa @@aut@@ Taichi Sakaguchi @@aut@@ Akiko Fujishima @@aut@@ Yohei Onodera @@aut@@ Kenichi Makino @@aut@@ Hiroshi Miura @@aut@@ Yukiyo Kumazawa @@aut@@ Kazumasa Takahashi @@aut@@ Yukihiro Terada @@aut@@ |
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Motonari Okabe misc RC648-665 misc QH471-489 misc confocal microscope misc embryonic development misc fluorescent probes misc live cell imaging misc two‐pronuclear stage misc Diseases of the endocrine glands. Clinical endocrinology misc Reproduction An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments |
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RC648-665 QH471-489 An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments confocal microscope embryonic development fluorescent probes live cell imaging two‐pronuclear stage |
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An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments |
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An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments |
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Motonari Okabe Hiromitsu Shirasawa Yuki Ono Mayumi Goto Takuya Iwasawa Taichi Sakaguchi Akiko Fujishima Yohei Onodera Kenichi Makino Hiroshi Miura Yukiyo Kumazawa Kazumasa Takahashi Yukihiro Terada |
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approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for dna, microtubules, and microfilaments |
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An approach for live imaging of first cleavage in mouse embryos using fluorescent chemical probes for DNA, microtubules, and microfilaments |
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Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. |
| abstractGer |
Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. |
| abstract_unstemmed |
Abstract Purpose Dynamic morphological changes in the chromosome and cytoskeleton occur in mammals and humans during early embryonic development, and abnormalities such as embryonic chromosomal aneuploidy occur when development does not proceed normally. Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. Results This method allowed us to observe how chromosome segregation errors in first somatic cell divisions in mouse embryos and enabled dynamic analysis of a phenomenon called lagging chromosomes. Conclusions By applying this method, we can observe any stage of embryonic development, which may provide new insights into embryonic development in other mammals. |
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Hiromitsu Shirasawa Yuki Ono Mayumi Goto Takuya Iwasawa Taichi Sakaguchi Akiko Fujishima Yohei Onodera Kenichi Makino Hiroshi Miura Yukiyo Kumazawa Kazumasa Takahashi Yukihiro Terada |
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2024-07-03T15:35:35.827Z |
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Visualization of the intracellular organelles and cytoskeleton allows elucidation of the development of early mammalian embryos. The behavior of the DNA and cytoskeleton in early mammalian embryos has conventionally been observed by injecting target molecule mRNAs, incorporating a fluorescent substance‐expressing gene, into embryos. In this study, we visualized the chronological behavior of male and female chromosome condensation in mouse embryos, beginning in the two‐pronuclear zygote, through the first division to the two‐cell stage, using fluorescent chemical probes to visualize the behavior of DNA, microtubules, and microfilaments. Method Mouse two‐pronuclear stage embryo were immersed in medium containing fluorescent chemical probes to visualize DNA, microtubules, and microfilaments. Observation was performed with a confocal microscope. 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