#121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got

Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Chia-Jung Li [verfasserIn] Wei-Che Lo [verfasserIn] Yen-Ju Sung [verfasserIn] Le-Tien Hsu [verfasserIn] Liang-Hsuan Chen [verfasserIn] Shang-Yu Huang [verfasserIn] Chia-Lin Chang [verfasserIn] Hong-Yuan Huang [verfasserIn] Chyi-Long Lee [verfasserIn] Yung-Kuei Sung [verfasserIn] Hsien-Ming Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Übergeordnetes Werk:	In: Fertility & Reproduction - World Scientific Publishing, 2021, 05(2023), 04, Seite 600-600
Übergeordnetes Werk:	volume:05 ; year:2023 ; number:04 ; pages:600-600

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1142/S2661318223743369

Katalog-ID:	DOAJ098674269

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spelling	10.1142/S2661318223743369 doi (DE-627)DOAJ098674269 (DE-599)DOAJ024f03f311ad4f7baa6a942262fefab7 DE-627 ger DE-627 rakwb eng QH471-489 Chia-Jung Li verfasserin aut #121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy. Reproduction Wei-Che Lo verfasserin aut Yen-Ju Sung verfasserin aut Le-Tien Hsu verfasserin aut Liang-Hsuan Chen verfasserin aut Shang-Yu Huang verfasserin aut Chia-Lin Chang verfasserin aut Hong-Yuan Huang verfasserin aut Chyi-Long Lee verfasserin aut Yung-Kuei Sung verfasserin aut Hsien-Ming Wu verfasserin aut In Fertility & Reproduction World Scientific Publishing, 2021 05(2023), 04, Seite 600-600 (DE-627)1745873104 26613174 nnns volume:05 year:2023 number:04 pages:600-600 https://doi.org/10.1142/S2661318223743369 kostenfrei https://doaj.org/article/024f03f311ad4f7baa6a942262fefab7 kostenfrei https://www.worldscientific.com/doi/10.1142/S2661318223743369 kostenfrei https://doaj.org/toc/2661-3182 Journal toc kostenfrei https://doaj.org/toc/2661-3174 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 05 2023 04 600-600
allfields_unstemmed	10.1142/S2661318223743369 doi (DE-627)DOAJ098674269 (DE-599)DOAJ024f03f311ad4f7baa6a942262fefab7 DE-627 ger DE-627 rakwb eng QH471-489 Chia-Jung Li verfasserin aut #121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy. Reproduction Wei-Che Lo verfasserin aut Yen-Ju Sung verfasserin aut Le-Tien Hsu verfasserin aut Liang-Hsuan Chen verfasserin aut Shang-Yu Huang verfasserin aut Chia-Lin Chang verfasserin aut Hong-Yuan Huang verfasserin aut Chyi-Long Lee verfasserin aut Yung-Kuei Sung verfasserin aut Hsien-Ming Wu verfasserin aut In Fertility & Reproduction World Scientific Publishing, 2021 05(2023), 04, Seite 600-600 (DE-627)1745873104 26613174 nnns volume:05 year:2023 number:04 pages:600-600 https://doi.org/10.1142/S2661318223743369 kostenfrei https://doaj.org/article/024f03f311ad4f7baa6a942262fefab7 kostenfrei https://www.worldscientific.com/doi/10.1142/S2661318223743369 kostenfrei https://doaj.org/toc/2661-3182 Journal toc kostenfrei https://doaj.org/toc/2661-3174 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 05 2023 04 600-600
allfieldsGer	10.1142/S2661318223743369 doi (DE-627)DOAJ098674269 (DE-599)DOAJ024f03f311ad4f7baa6a942262fefab7 DE-627 ger DE-627 rakwb eng QH471-489 Chia-Jung Li verfasserin aut #121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy. Reproduction Wei-Che Lo verfasserin aut Yen-Ju Sung verfasserin aut Le-Tien Hsu verfasserin aut Liang-Hsuan Chen verfasserin aut Shang-Yu Huang verfasserin aut Chia-Lin Chang verfasserin aut Hong-Yuan Huang verfasserin aut Chyi-Long Lee verfasserin aut Yung-Kuei Sung verfasserin aut Hsien-Ming Wu verfasserin aut In Fertility & Reproduction World Scientific Publishing, 2021 05(2023), 04, Seite 600-600 (DE-627)1745873104 26613174 nnns volume:05 year:2023 number:04 pages:600-600 https://doi.org/10.1142/S2661318223743369 kostenfrei https://doaj.org/article/024f03f311ad4f7baa6a942262fefab7 kostenfrei https://www.worldscientific.com/doi/10.1142/S2661318223743369 kostenfrei https://doaj.org/toc/2661-3182 Journal toc kostenfrei https://doaj.org/toc/2661-3174 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 05 2023 04 600-600
allfieldsSound	10.1142/S2661318223743369 doi (DE-627)DOAJ098674269 (DE-599)DOAJ024f03f311ad4f7baa6a942262fefab7 DE-627 ger DE-627 rakwb eng QH471-489 Chia-Jung Li verfasserin aut #121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy. Reproduction Wei-Che Lo verfasserin aut Yen-Ju Sung verfasserin aut Le-Tien Hsu verfasserin aut Liang-Hsuan Chen verfasserin aut Shang-Yu Huang verfasserin aut Chia-Lin Chang verfasserin aut Hong-Yuan Huang verfasserin aut Chyi-Long Lee verfasserin aut Yung-Kuei Sung verfasserin aut Hsien-Ming Wu verfasserin aut In Fertility & Reproduction World Scientific Publishing, 2021 05(2023), 04, Seite 600-600 (DE-627)1745873104 26613174 nnns volume:05 year:2023 number:04 pages:600-600 https://doi.org/10.1142/S2661318223743369 kostenfrei https://doaj.org/article/024f03f311ad4f7baa6a942262fefab7 kostenfrei https://www.worldscientific.com/doi/10.1142/S2661318223743369 kostenfrei https://doaj.org/toc/2661-3182 Journal toc kostenfrei https://doaj.org/toc/2661-3174 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 05 2023 04 600-600
language	English
source	In Fertility & Reproduction 05(2023), 04, Seite 600-600 volume:05 year:2023 number:04 pages:600-600
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authorswithroles_txt_mv	Chia-Jung Li @@aut@@ Wei-Che Lo @@aut@@ Yen-Ju Sung @@aut@@ Le-Tien Hsu @@aut@@ Liang-Hsuan Chen @@aut@@ Shang-Yu Huang @@aut@@ Chia-Lin Chang @@aut@@ Hong-Yuan Huang @@aut@@ Chyi-Long Lee @@aut@@ Yung-Kuei Sung @@aut@@ Hsien-Ming Wu @@aut@@
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topic_title	QH471-489 #121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got
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title	#121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got
ctrlnum	(DE-627)DOAJ098674269 (DE-599)DOAJ024f03f311ad4f7baa6a942262fefab7
title_full	#121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got
author_sort	Chia-Jung Li
journal	Fertility & Reproduction
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author_browse	Chia-Jung Li Wei-Che Lo Yen-Ju Sung Le-Tien Hsu Liang-Hsuan Chen Shang-Yu Huang Chia-Lin Chang Hong-Yuan Huang Chyi-Long Lee Yung-Kuei Sung Hsien-Ming Wu
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title_sort	#121 : the easier blastocyst biopsy, the more trophectoderm cells we got
callnumber	QH471-489
title_auth	#121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got
abstract	Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy.
abstractGer	Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy.
abstract_unstemmed	Background and Aims: Several studies showed various factors associated with pregnancy outcomes after the euploid embryo transfer, including parental, endometrial, and embryo biopsy. The consensus of the most suitable biopsied cell number is 5-10 cells among IVF labs. Therefore, for embryologists, how to have a steadily manipulated blastocyst to get an appropriate biopsied trophectoderm cell number is crucial. However, the factors related to biopsy manipulation are still being determined. Here, we retrospectively analyze the DNA concentrations from biopsied cells to test the hypothesis TE grading, hatching status, and biopsy day correlated with embryologists’ biopsy handling. Method: A retrospective study enrolled 212 blastocysts from 49 PGT-A cycles (NGS platform) from September 2021 and March 2023 at the Fertility and Reproductive Genetic Center of Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan. All the TE cells were removed by mechanical cutting by embryologists following the lab standard operating procedure. This study excluded five blastocysts (2.4%, 5/212) for the unsuccessful whole genome amplification (WGA), i.e., DNA concentration was less than 10 ng/[Formula: see text] l. Results: No difference in average DNA concentration was found between embryo ploidy status (Fig. A, P=0.8), embryologist (Fig. A, P=0.65), and TE grading (Gardner TE grading B vs. C, P=0.8). However, combined hatching status and day of the biopsy revealed a significantly higher DNA concentration in the hatching Day 5 to Day 7 groups than in the hatched Day 5 to Day 7 group (Fig. B, P[Formula: see text]0.0001). Conclusion: Our data suggest that the embryo ploidy status and Gardner grading of TE cells did not affect embryologists’ steadily performing biopsies. Interestingly, the easier biopsy, the more TE cells we got. This result implies that the hatching (Gardner expansion grade 5) embryo has a higher potential for damage after trophectoderm biopsy.
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title_short	#121 : The Easier Blastocyst Biopsy, The More Trophectoderm Cells We Got
url	https://doi.org/10.1142/S2661318223743369 https://doaj.org/article/024f03f311ad4f7baa6a942262fefab7 https://www.worldscientific.com/doi/10.1142/S2661318223743369 https://doaj.org/toc/2661-3182 https://doaj.org/toc/2661-3174
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author2	Wei-Che Lo Yen-Ju Sung Le-Tien Hsu Liang-Hsuan Chen Shang-Yu Huang Chia-Lin Chang Hong-Yuan Huang Chyi-Long Lee Yung-Kuei Sung Hsien-Ming Wu
author2Str	Wei-Che Lo Yen-Ju Sung Le-Tien Hsu Liang-Hsuan Chen Shang-Yu Huang Chia-Lin Chang Hong-Yuan Huang Chyi-Long Lee Yung-Kuei Sung Hsien-Ming Wu
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up_date	2024-07-03T18:33:54.686Z
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