Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum
Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
E. A. Trofimova [verfasserIn] S. V. Kononova [verfasserIn] I. N. Shumilova [verfasserIn] B. L. Manin [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch ; Russisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Ветеринария сегодня - Da Vinci Media, 2019, 12(2023), 4, Seite 345-353 |
|---|---|
| Übergeordnetes Werk: |
volume:12 ; year:2023 ; number:4 ; pages:345-353 |
| Links: |
Link aufrufen |
|---|
| DOI / URN: |
10.29326/2304-196X-2023-12-4-345-353 |
|---|
| Katalog-ID: |
DOAJ09871645X |
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| 520 | |a Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin. | ||
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10.29326/2304-196X-2023-12-4-345-353 doi (DE-627)DOAJ09871645X (DE-599)DOAJfb246f8ee0d4473e83e9337f90650441 DE-627 ger DE-627 rakwb eng rus SF600-1100 E. A. Trofimova verfasserin aut Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin. continuous cell line proliferative activity immortalization cytopathic effect infectious activity Veterinary medicine S. V. Kononova verfasserin aut I. N. Shumilova verfasserin aut B. L. Manin verfasserin aut In Ветеринария сегодня Da Vinci Media, 2019 12(2023), 4, Seite 345-353 (DE-627)1760602620 26586959 nnns volume:12 year:2023 number:4 pages:345-353 https://doi.org/10.29326/2304-196X-2023-12-4-345-353 kostenfrei https://doaj.org/article/fb246f8ee0d4473e83e9337f90650441 kostenfrei https://veterinary.arriah.ru/jour/article/view/771 kostenfrei https://doaj.org/toc/2304-196X Journal toc kostenfrei https://doaj.org/toc/2658-6959 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 12 2023 4 345-353 |
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10.29326/2304-196X-2023-12-4-345-353 doi (DE-627)DOAJ09871645X (DE-599)DOAJfb246f8ee0d4473e83e9337f90650441 DE-627 ger DE-627 rakwb eng rus SF600-1100 E. A. Trofimova verfasserin aut Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin. continuous cell line proliferative activity immortalization cytopathic effect infectious activity Veterinary medicine S. V. Kononova verfasserin aut I. N. Shumilova verfasserin aut B. L. Manin verfasserin aut In Ветеринария сегодня Da Vinci Media, 2019 12(2023), 4, Seite 345-353 (DE-627)1760602620 26586959 nnns volume:12 year:2023 number:4 pages:345-353 https://doi.org/10.29326/2304-196X-2023-12-4-345-353 kostenfrei https://doaj.org/article/fb246f8ee0d4473e83e9337f90650441 kostenfrei https://veterinary.arriah.ru/jour/article/view/771 kostenfrei https://doaj.org/toc/2304-196X Journal toc kostenfrei https://doaj.org/toc/2658-6959 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 12 2023 4 345-353 |
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S - Agriculture |
| author |
E. A. Trofimova |
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E. A. Trofimova misc SF600-1100 misc continuous cell line misc proliferative activity misc immortalization misc cytopathic effect misc infectious activity misc Veterinary medicine Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum |
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SF600-1100 Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum continuous cell line proliferative activity immortalization cytopathic effect infectious activity |
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Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum |
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Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum |
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E. A. Trofimova |
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E. A. Trofimova S. V. Kononova I. N. Shumilova B. L. Manin |
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10.29326/2304-196X-2023-12-4-345-353 |
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goat-derived cell line (<i<capra hircus</i<) tch generated by karyological and morphological transformation of yаdk-04 ccl during subcultivation with lanthanide-treated bovine serum |
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SF600-1100 |
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Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum |
| abstract |
Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin. |
| abstractGer |
Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin. |
| abstract_unstemmed |
Ability of the continuous cell lines to evolve enables generation of new transformed cell cultures with unlimited life potential and different from the original prototypes in the process of sequential cultivation. There are practically no universal mechanisms and methods for new cell line generation. But it was noted that cell immortalization is associated with chromosomal rearrangements (chromatid morphology) and changes in the number of chromosomes. The paper presents the results of the generation of a new Testis Capra hircus(TCh) cell line, suitable for effective replication of dermatotropic and other types of animal viruses, in order to scale up viral material used for the manufacture of the means for animal disease specific prevention and diagnosis. The monolayer TCh cell line was transformed from the continuous YаDK-04 cell line as a result of more than 50 passages in the growth medium supplemented with 10% of lanthanide-treated bovine serum. Use of the bovine serum purified and supplemented with lanthanides during the cultivation of the continuous cell line YаDK-04 led to significant chromosomal rearrangements and contributed to the formation of a stable and productive new TCh cell line, which differed in cytomorphological and karyological characteristics and had unlimited potential for passaging without changing the cell karyotype and morphology. The novel continuous cell line proved to be suitable for effective reproduction of such disease pathogens as lumpy skin disease, sheep pox, peste des petits ruminants agents. These are mainly viruses of dermatotropic origin. |
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Goat-derived cell line (<i<Capra hircus</i<) TCh generated by karyological and morphological transformation of YаDK-04 CCL during subcultivation with lanthanide-treated bovine serum |
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https://doi.org/10.29326/2304-196X-2023-12-4-345-353 https://doaj.org/article/fb246f8ee0d4473e83e9337f90650441 https://veterinary.arriah.ru/jour/article/view/771 https://doaj.org/toc/2304-196X https://doaj.org/toc/2658-6959 |
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S. V. Kononova I. N. Shumilova B. L. Manin |
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2024-07-03T18:48:05.611Z |
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