A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection

Enrofloxacin, a fluoroquinolone widely used in animal husbandry, presents environmental and human health hazards due to its stability and incomplete hydrolysis leading to residue accumulation. To address this concern, a highly sensitive aptamer biosensor utilizing a localized surface plasmon resonan...
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Autor*in:	Pan Wang [verfasserIn] Liyun Ding [verfasserIn] Yumei Zhang [verfasserIn] Xingdong Jiang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	enrofloxacin AuNPs-Apt complex local surface plasmon resonance sensing chip

Übergeordnetes Werk:	In: Biosensors - MDPI AG, 2012, 13(2023), 12, p 1027
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:12, p 1027

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/bios13121027

Katalog-ID:	DOAJ098903454

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520			\|a Enrofloxacin, a fluoroquinolone widely used in animal husbandry, presents environmental and human health hazards due to its stability and incomplete hydrolysis leading to residue accumulation. To address this concern, a highly sensitive aptamer biosensor utilizing a localized surface plasmon resonance (LSPR) sensing chip and microfluidic technology was developed for rapid enrofloxacin residue detection. AuNPs were prepared by the seed method and the AuNPs-Apt complexes were immobilized on the chip by the sulfhydryl groups modified on the end of the aptamer. The properties and morphologies of the sensing chip and AuNPs-Apt complexes were characterized by Fourier transform infrared spectroscopy (FTIR), UV-Vis spectrophotometer, and scanning electron microscope (SEM), respectively. The sensing chip was able to detect enrofloxacin in the range of 0.01–100 ng/mL with good linearity, and the relationship between the response of the sensing chip and the concentration was Δλ (nm) = 1.288log ConENR (ng/mL) + 5.245 (R<sup<2</sup< = 0.99), with the limit of detection being 0.001 ng/mL. The anti-interference, repeatability, and selectivity of this sensing chip were studied in detail. Compared with other sensors, this novel aptamer biosensor based on AuNPs-Apt complexes is expected to achieve simple, stable, and economical application in the field of enrofloxacin detection.
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language	English
source	In Biosensors 13(2023), 12, p 1027 volume:13 year:2023 number:12, p 1027
sourceStr	In Biosensors 13(2023), 12, p 1027 volume:13 year:2023 number:12, p 1027
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	enrofloxacin AuNPs-Apt complex local surface plasmon resonance sensing chip Biotechnology
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authorswithroles_txt_mv	Pan Wang @@aut@@ Liyun Ding @@aut@@ Yumei Zhang @@aut@@ Xingdong Jiang @@aut@@
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callnumber-first	T - Technology
author	Pan Wang
spellingShingle	Pan Wang misc TP248.13-248.65 misc enrofloxacin misc AuNPs-Apt complex misc local surface plasmon resonance misc sensing chip misc Biotechnology A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection
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topic_title	TP248.13-248.65 A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection enrofloxacin AuNPs-Apt complex local surface plasmon resonance sensing chip
topic	misc TP248.13-248.65 misc enrofloxacin misc AuNPs-Apt complex misc local surface plasmon resonance misc sensing chip misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc enrofloxacin misc AuNPs-Apt complex misc local surface plasmon resonance misc sensing chip misc Biotechnology
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title	A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection
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title_full	A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection
author_sort	Pan Wang
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author_browse	Pan Wang Liyun Ding Yumei Zhang Xingdong Jiang
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title_sort	novel aptamer biosensor based on a localized surface plasmon resonance sensing chip for high-sensitivity and rapid enrofloxacin detection
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title_auth	A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection
abstract	Enrofloxacin, a fluoroquinolone widely used in animal husbandry, presents environmental and human health hazards due to its stability and incomplete hydrolysis leading to residue accumulation. To address this concern, a highly sensitive aptamer biosensor utilizing a localized surface plasmon resonance (LSPR) sensing chip and microfluidic technology was developed for rapid enrofloxacin residue detection. AuNPs were prepared by the seed method and the AuNPs-Apt complexes were immobilized on the chip by the sulfhydryl groups modified on the end of the aptamer. The properties and morphologies of the sensing chip and AuNPs-Apt complexes were characterized by Fourier transform infrared spectroscopy (FTIR), UV-Vis spectrophotometer, and scanning electron microscope (SEM), respectively. The sensing chip was able to detect enrofloxacin in the range of 0.01–100 ng/mL with good linearity, and the relationship between the response of the sensing chip and the concentration was Δλ (nm) = 1.288log ConENR (ng/mL) + 5.245 (R<sup<2</sup< = 0.99), with the limit of detection being 0.001 ng/mL. The anti-interference, repeatability, and selectivity of this sensing chip were studied in detail. Compared with other sensors, this novel aptamer biosensor based on AuNPs-Apt complexes is expected to achieve simple, stable, and economical application in the field of enrofloxacin detection.
abstractGer	Enrofloxacin, a fluoroquinolone widely used in animal husbandry, presents environmental and human health hazards due to its stability and incomplete hydrolysis leading to residue accumulation. To address this concern, a highly sensitive aptamer biosensor utilizing a localized surface plasmon resonance (LSPR) sensing chip and microfluidic technology was developed for rapid enrofloxacin residue detection. AuNPs were prepared by the seed method and the AuNPs-Apt complexes were immobilized on the chip by the sulfhydryl groups modified on the end of the aptamer. The properties and morphologies of the sensing chip and AuNPs-Apt complexes were characterized by Fourier transform infrared spectroscopy (FTIR), UV-Vis spectrophotometer, and scanning electron microscope (SEM), respectively. The sensing chip was able to detect enrofloxacin in the range of 0.01–100 ng/mL with good linearity, and the relationship between the response of the sensing chip and the concentration was Δλ (nm) = 1.288log ConENR (ng/mL) + 5.245 (R<sup<2</sup< = 0.99), with the limit of detection being 0.001 ng/mL. The anti-interference, repeatability, and selectivity of this sensing chip were studied in detail. Compared with other sensors, this novel aptamer biosensor based on AuNPs-Apt complexes is expected to achieve simple, stable, and economical application in the field of enrofloxacin detection.
abstract_unstemmed	Enrofloxacin, a fluoroquinolone widely used in animal husbandry, presents environmental and human health hazards due to its stability and incomplete hydrolysis leading to residue accumulation. To address this concern, a highly sensitive aptamer biosensor utilizing a localized surface plasmon resonance (LSPR) sensing chip and microfluidic technology was developed for rapid enrofloxacin residue detection. AuNPs were prepared by the seed method and the AuNPs-Apt complexes were immobilized on the chip by the sulfhydryl groups modified on the end of the aptamer. The properties and morphologies of the sensing chip and AuNPs-Apt complexes were characterized by Fourier transform infrared spectroscopy (FTIR), UV-Vis spectrophotometer, and scanning electron microscope (SEM), respectively. The sensing chip was able to detect enrofloxacin in the range of 0.01–100 ng/mL with good linearity, and the relationship between the response of the sensing chip and the concentration was Δλ (nm) = 1.288log ConENR (ng/mL) + 5.245 (R<sup<2</sup< = 0.99), with the limit of detection being 0.001 ng/mL. The anti-interference, repeatability, and selectivity of this sensing chip were studied in detail. Compared with other sensors, this novel aptamer biosensor based on AuNPs-Apt complexes is expected to achieve simple, stable, and economical application in the field of enrofloxacin detection.
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title_short	A Novel Aptamer Biosensor Based on a Localized Surface Plasmon Resonance Sensing Chip for High-Sensitivity and Rapid Enrofloxacin Detection
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