Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways

The heterogeneous nature of human breast cancer (HBC) can still lead to therapy inefficacy and high lethality, and new therapeutics as well as new spontaneous animal models are needed to benefit translational HBC research. Dogs are primarily investigated since they spontaneously develop tumors that...
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Autor*in:	Alessandro Sammarco [verfasserIn] Giorgia Beffagna [verfasserIn] Roberta Sacchetto [verfasserIn] Andrea Vettori [verfasserIn] Federico Bonsembiante [verfasserIn] Giulia Scarin [verfasserIn] Maria Elena Gelain [verfasserIn] Laura Cavicchioli [verfasserIn] Silvia Ferro [verfasserIn] Cristina Geroni [verfasserIn] Paolo Lombardi [verfasserIn] Valentina Zappulli [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	berberine derivates dog mammary tumors zebrafish Hippo Wnt

Übergeordnetes Werk:	In: Biomedicines - MDPI AG, 2014, 11(2023), 12, p 3317
Übergeordnetes Werk:	volume:11 ; year:2023 ; number:12, p 3317

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biomedicines11123317

Katalog-ID:	DOAJ09890633X

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callnumber-first	Q - Science
author	Alessandro Sammarco
spellingShingle	Alessandro Sammarco misc QH301-705.5 misc berberine derivates misc dog misc mammary tumors misc zebrafish misc Hippo misc Wnt misc Biology (General) Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways
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topic_title	QH301-705.5 Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways berberine derivates dog mammary tumors zebrafish Hippo Wnt
topic	misc QH301-705.5 misc berberine derivates misc dog misc mammary tumors misc zebrafish misc Hippo misc Wnt misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc berberine derivates misc dog misc mammary tumors misc zebrafish misc Hippo misc Wnt misc Biology (General)
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title	Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways
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title_full	Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways
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author_browse	Alessandro Sammarco Giorgia Beffagna Roberta Sacchetto Andrea Vettori Federico Bonsembiante Giulia Scarin Maria Elena Gelain Laura Cavicchioli Silvia Ferro Cristina Geroni Paolo Lombardi Valentina Zappulli
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title_sort	antitumor effect of berberine analogs in a canine mammary tumor cell line and in zebrafish reporters via wnt/β-catenin and hippo pathways
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title_auth	Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways
abstract	The heterogeneous nature of human breast cancer (HBC) can still lead to therapy inefficacy and high lethality, and new therapeutics as well as new spontaneous animal models are needed to benefit translational HBC research. Dogs are primarily investigated since they spontaneously develop tumors that share many features with human cancers. In recent years, different natural phytochemicals including berberine, a plant alkaloid, have been reported to have antiproliferative activity in vitro in human cancers and rodent animal models. In this study, we report the antiproliferative activity and mechanism of action of berberine, its active metabolite berberrubine, and eight analogs, on a canine mammary carcinoma cell line and in transgenic zebrafish models. We demonstrate both in vitro and in vivo the significant effects of specific analogs on cell viability via the induction of apoptosis, also identifying their role in inhibiting the Wnt/β-catenin pathway and activating the Hippo signals with a downstream reduction in <i<CTGF</i< expression. In particular, the berberine analogs NAX035 and NAX057 show the highest therapeutic efficacy, deserving further analyses to elucidate their mechanism of action more in detail, and in vivo studies on spontaneous neoplastic diseases are needed, aiming at improving veterinary treatments of cancer as well as translational cancer research.
abstractGer	The heterogeneous nature of human breast cancer (HBC) can still lead to therapy inefficacy and high lethality, and new therapeutics as well as new spontaneous animal models are needed to benefit translational HBC research. Dogs are primarily investigated since they spontaneously develop tumors that share many features with human cancers. In recent years, different natural phytochemicals including berberine, a plant alkaloid, have been reported to have antiproliferative activity in vitro in human cancers and rodent animal models. In this study, we report the antiproliferative activity and mechanism of action of berberine, its active metabolite berberrubine, and eight analogs, on a canine mammary carcinoma cell line and in transgenic zebrafish models. We demonstrate both in vitro and in vivo the significant effects of specific analogs on cell viability via the induction of apoptosis, also identifying their role in inhibiting the Wnt/β-catenin pathway and activating the Hippo signals with a downstream reduction in <i<CTGF</i< expression. In particular, the berberine analogs NAX035 and NAX057 show the highest therapeutic efficacy, deserving further analyses to elucidate their mechanism of action more in detail, and in vivo studies on spontaneous neoplastic diseases are needed, aiming at improving veterinary treatments of cancer as well as translational cancer research.
abstract_unstemmed	The heterogeneous nature of human breast cancer (HBC) can still lead to therapy inefficacy and high lethality, and new therapeutics as well as new spontaneous animal models are needed to benefit translational HBC research. Dogs are primarily investigated since they spontaneously develop tumors that share many features with human cancers. In recent years, different natural phytochemicals including berberine, a plant alkaloid, have been reported to have antiproliferative activity in vitro in human cancers and rodent animal models. In this study, we report the antiproliferative activity and mechanism of action of berberine, its active metabolite berberrubine, and eight analogs, on a canine mammary carcinoma cell line and in transgenic zebrafish models. We demonstrate both in vitro and in vivo the significant effects of specific analogs on cell viability via the induction of apoptosis, also identifying their role in inhibiting the Wnt/β-catenin pathway and activating the Hippo signals with a downstream reduction in <i<CTGF</i< expression. In particular, the berberine analogs NAX035 and NAX057 show the highest therapeutic efficacy, deserving further analyses to elucidate their mechanism of action more in detail, and in vivo studies on spontaneous neoplastic diseases are needed, aiming at improving veterinary treatments of cancer as well as translational cancer research.
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title_short	Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways
url	https://doi.org/10.3390/biomedicines11123317 https://doaj.org/article/8f7540fa1e4c43f4a994d739d2f4accc https://www.mdpi.com/2227-9059/11/12/3317 https://doaj.org/toc/2227-9059
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author2	Giorgia Beffagna Roberta Sacchetto Andrea Vettori Federico Bonsembiante Giulia Scarin Maria Elena Gelain Laura Cavicchioli Silvia Ferro Cristina Geroni Paolo Lombardi Valentina Zappulli
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up_date	2024-07-03T19:54:38.242Z
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Antitumor Effect of Berberine Analogs in a Canine Mammary Tumor Cell Line and in Zebrafish Reporters via Wnt/β-Catenin and Hippo Pathways

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