Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda)

The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Kinga Mazurkiewicz-Zapałowicz [verfasserIn] Bogumiła Pilarczyk [verfasserIn] Lidia Kołodziejczyk [verfasserIn] Cezary Tkaczuk [verfasserIn] Magdalena Twarużek [verfasserIn] Łukasz Łopusiewicz [verfasserIn] Jan Grajewski [verfasserIn] Ewa Dzika [verfasserIn] Elżbieta Kalisińska [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	ovistatic effect entomopathogenic fungi biocontrol

Übergeordnetes Werk:	In: Animals - MDPI AG, 2011, 13(2023), 24, p 3782
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:24, p 3782

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ani13243782

Katalog-ID:	DOAJ098923293

Internformat


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520			\|a The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<).
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700	0		\|a Lidia Kołodziejczyk \|e verfasserin \|4 aut
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700	0		\|a Magdalena Twarużek \|e verfasserin \|4 aut
700	0		\|a Łukasz Łopusiewicz \|e verfasserin \|4 aut
700	0		\|a Jan Grajewski \|e verfasserin \|4 aut
700	0		\|a Ewa Dzika \|e verfasserin \|4 aut
700	0		\|a Elżbieta Kalisińska \|e verfasserin \|4 aut
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912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
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912			\|a GBV_ILN_73
912			\|a GBV_ILN_95
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912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
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912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
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912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
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912			\|a GBV_ILN_2061
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912			\|a GBV_ILN_2190
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 13 \|j 2023 \|e 24, p 3782

Indexfelder

author_variant	k m z kmz b p bp l k lk c t ct m t mt ł ł łł j g jg e d ed e k ek
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allfields	10.3390/ani13243782 doi (DE-627)DOAJ098923293 (DE-599)DOAJfd5214f2d06d49469c45f16cdb0f70b2 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Kinga Mazurkiewicz-Zapałowicz verfasserin aut Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<). <i<Ascaris suum</i< ovistatic effect entomopathogenic fungi biocontrol Veterinary medicine Zoology Bogumiła Pilarczyk verfasserin aut Lidia Kołodziejczyk verfasserin aut Cezary Tkaczuk verfasserin aut Magdalena Twarużek verfasserin aut Łukasz Łopusiewicz verfasserin aut Jan Grajewski verfasserin aut Ewa Dzika verfasserin aut Elżbieta Kalisińska verfasserin aut In Animals MDPI AG, 2011 13(2023), 24, p 3782 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:24, p 3782 https://doi.org/10.3390/ani13243782 kostenfrei https://doaj.org/article/fd5214f2d06d49469c45f16cdb0f70b2 kostenfrei https://www.mdpi.com/2076-2615/13/24/3782 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 24, p 3782
spelling	10.3390/ani13243782 doi (DE-627)DOAJ098923293 (DE-599)DOAJfd5214f2d06d49469c45f16cdb0f70b2 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Kinga Mazurkiewicz-Zapałowicz verfasserin aut Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<). <i<Ascaris suum</i< ovistatic effect entomopathogenic fungi biocontrol Veterinary medicine Zoology Bogumiła Pilarczyk verfasserin aut Lidia Kołodziejczyk verfasserin aut Cezary Tkaczuk verfasserin aut Magdalena Twarużek verfasserin aut Łukasz Łopusiewicz verfasserin aut Jan Grajewski verfasserin aut Ewa Dzika verfasserin aut Elżbieta Kalisińska verfasserin aut In Animals MDPI AG, 2011 13(2023), 24, p 3782 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:24, p 3782 https://doi.org/10.3390/ani13243782 kostenfrei https://doaj.org/article/fd5214f2d06d49469c45f16cdb0f70b2 kostenfrei https://www.mdpi.com/2076-2615/13/24/3782 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 24, p 3782
allfields_unstemmed	10.3390/ani13243782 doi (DE-627)DOAJ098923293 (DE-599)DOAJfd5214f2d06d49469c45f16cdb0f70b2 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Kinga Mazurkiewicz-Zapałowicz verfasserin aut Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<). <i<Ascaris suum</i< ovistatic effect entomopathogenic fungi biocontrol Veterinary medicine Zoology Bogumiła Pilarczyk verfasserin aut Lidia Kołodziejczyk verfasserin aut Cezary Tkaczuk verfasserin aut Magdalena Twarużek verfasserin aut Łukasz Łopusiewicz verfasserin aut Jan Grajewski verfasserin aut Ewa Dzika verfasserin aut Elżbieta Kalisińska verfasserin aut In Animals MDPI AG, 2011 13(2023), 24, p 3782 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:24, p 3782 https://doi.org/10.3390/ani13243782 kostenfrei https://doaj.org/article/fd5214f2d06d49469c45f16cdb0f70b2 kostenfrei https://www.mdpi.com/2076-2615/13/24/3782 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 24, p 3782
allfieldsGer	10.3390/ani13243782 doi (DE-627)DOAJ098923293 (DE-599)DOAJfd5214f2d06d49469c45f16cdb0f70b2 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Kinga Mazurkiewicz-Zapałowicz verfasserin aut Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<). <i<Ascaris suum</i< ovistatic effect entomopathogenic fungi biocontrol Veterinary medicine Zoology Bogumiła Pilarczyk verfasserin aut Lidia Kołodziejczyk verfasserin aut Cezary Tkaczuk verfasserin aut Magdalena Twarużek verfasserin aut Łukasz Łopusiewicz verfasserin aut Jan Grajewski verfasserin aut Ewa Dzika verfasserin aut Elżbieta Kalisińska verfasserin aut In Animals MDPI AG, 2011 13(2023), 24, p 3782 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:24, p 3782 https://doi.org/10.3390/ani13243782 kostenfrei https://doaj.org/article/fd5214f2d06d49469c45f16cdb0f70b2 kostenfrei https://www.mdpi.com/2076-2615/13/24/3782 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 24, p 3782
allfieldsSound	10.3390/ani13243782 doi (DE-627)DOAJ098923293 (DE-599)DOAJfd5214f2d06d49469c45f16cdb0f70b2 DE-627 ger DE-627 rakwb eng SF600-1100 QL1-991 Kinga Mazurkiewicz-Zapałowicz verfasserin aut Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<). <i<Ascaris suum</i< ovistatic effect entomopathogenic fungi biocontrol Veterinary medicine Zoology Bogumiła Pilarczyk verfasserin aut Lidia Kołodziejczyk verfasserin aut Cezary Tkaczuk verfasserin aut Magdalena Twarużek verfasserin aut Łukasz Łopusiewicz verfasserin aut Jan Grajewski verfasserin aut Ewa Dzika verfasserin aut Elżbieta Kalisińska verfasserin aut In Animals MDPI AG, 2011 13(2023), 24, p 3782 (DE-627)657589306 (DE-600)2606558-7 20762615 nnns volume:13 year:2023 number:24, p 3782 https://doi.org/10.3390/ani13243782 kostenfrei https://doaj.org/article/fd5214f2d06d49469c45f16cdb0f70b2 kostenfrei https://www.mdpi.com/2076-2615/13/24/3782 kostenfrei https://doaj.org/toc/2076-2615 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2023 24, p 3782
language	English
source	In Animals 13(2023), 24, p 3782 volume:13 year:2023 number:24, p 3782
sourceStr	In Animals 13(2023), 24, p 3782 volume:13 year:2023 number:24, p 3782
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Ascaris suum</i< ovistatic effect entomopathogenic fungi biocontrol Veterinary medicine Zoology
isfreeaccess_bool	true
container_title	Animals
authorswithroles_txt_mv	Kinga Mazurkiewicz-Zapałowicz @@aut@@ Bogumiła Pilarczyk @@aut@@ Lidia Kołodziejczyk @@aut@@ Cezary Tkaczuk @@aut@@ Magdalena Twarużek @@aut@@ Łukasz Łopusiewicz @@aut@@ Jan Grajewski @@aut@@ Ewa Dzika @@aut@@ Elżbieta Kalisińska @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	657589306
id	DOAJ098923293
language_de	englisch
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callnumber-first	S - Agriculture
author	Kinga Mazurkiewicz-Zapałowicz
spellingShingle	Kinga Mazurkiewicz-Zapałowicz misc SF600-1100 misc QL1-991 misc <i<Ascaris suum</i< misc ovistatic effect misc entomopathogenic fungi misc biocontrol misc Veterinary medicine misc Zoology Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda)
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topic_title	SF600-1100 QL1-991 Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda) <i<Ascaris suum</i< ovistatic effect entomopathogenic fungi biocontrol
topic	misc SF600-1100 misc QL1-991 misc <i<Ascaris suum</i< misc ovistatic effect misc entomopathogenic fungi misc biocontrol misc Veterinary medicine misc Zoology
topic_unstemmed	misc SF600-1100 misc QL1-991 misc <i<Ascaris suum</i< misc ovistatic effect misc entomopathogenic fungi misc biocontrol misc Veterinary medicine misc Zoology
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title	Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda)
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title_full	Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda)
author_sort	Kinga Mazurkiewicz-Zapałowicz
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author_browse	Kinga Mazurkiewicz-Zapałowicz Bogumiła Pilarczyk Lidia Kołodziejczyk Cezary Tkaczuk Magdalena Twarużek Łukasz Łopusiewicz Jan Grajewski Ewa Dzika Elżbieta Kalisińska
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author-letter	Kinga Mazurkiewicz-Zapałowicz
doi_str_mv	10.3390/ani13243782
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title_sort	effect of selected entomopathogenic fungal species on embryonic development of <i<ascaris suum</i< (nematoda)
callnumber	SF600-1100
title_auth	Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda)
abstract	The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<).
abstractGer	The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<).
abstract_unstemmed	The aim of the study was to evaluate the potential of using five selected species of entomopathogenic fungi (<i<Beauveria bassiana</i<, <i<B. brongniartii</i<, <i<Conidiobolus coronatus</i<, <i<Isaria fumosorosea</i<, and <i<Metarhizium robertsii</i<) in the bioregulation of the dispersive stages of the parasitic nematode—<i<Ascaris suum</i<. Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). The absence of morphological changes in the <i<A. suum</i< egg shells suggests that the antagonistic effect of the studied entomopathogenic fungi may be due to their cytotoxicity, associated with the production of secondary metabolites—toxins (<i<M. robertsii</i<) and enzymatic activity (<i<I. fumosorosea</i<).
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container_issue	24, p 3782
title_short	Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda)
url	https://doi.org/10.3390/ani13243782 https://doaj.org/article/fd5214f2d06d49469c45f16cdb0f70b2 https://www.mdpi.com/2076-2615/13/24/3782 https://doaj.org/toc/2076-2615
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Experimental cultures of each of the selected entomopathogenic fungi, as well as a control culture without fungi, were incubated with <i<A. suum</i< eggs at 26 °C for 28 days. Development of the <i<A. suum</i< eggs was observed using a light microscope on the 7th, 14th, 21st, and 28th days of incubation. The API-ZYM<sup<®</sup< test was used to determine, semiquantitatively, the activity of 19 hydrolytic enzymes from the entomopathogenic fungi. The cytotoxicity of the fungi was determined using tetrazole salt MTT. It was found that none of the five tested strains of entomopathogenic fungi showed an ovicidal effect, and none of them colonized the <i<A. suum</i< egg shells. However, ovistatic activity was observed mainly until the 14th day of incubation by <i<I. fumosorosea</i<, <i<M. robertsii</i<, and <i<B. bassiana</i<. In the MTT test, <i<M. robertsii</i< showed moderate cytotoxicity, while the other species showed low cytotoxicity. Among the strains tested, <i<I. fumosorosea</i< showed the highest spectrum of hydrolase production (13 out of 19 enzymes gave a positive reaction from 3 to 5; 20–40 nM or more). 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Effect of Selected Entomopathogenic Fungal Species on Embryonic Development of <i<Ascaris suum</i< (Nematoda)

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