Development and implementation of natural killer cell simultaneous ADCC and direct killing assay
Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK...
Ausführliche Beschreibung
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| Autor*in: |
Maia M.C. Bennett-Boehm [verfasserIn] Anna R. Mahr [verfasserIn] Sean T. Hartwell [verfasserIn] Alexander K. Regan [verfasserIn] Isabelle S. Weber [verfasserIn] Arriana Blackmon [verfasserIn] Cami R. Bisson [verfasserIn] Angela N. Truong [verfasserIn] Bella A. Circo [verfasserIn] Jaden Nienhueser [verfasserIn] Donald R. Rogers [verfasserIn] Nathan Booher [verfasserIn] Nithya Rajagopalan [verfasserIn] Jacob W.S. Martens [verfasserIn] Paul W. Denton [verfasserIn] |
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Heliyon - Elsevier, 2016, 9(2023), 12, Seite e22991- |
|---|---|
| Übergeordnetes Werk: |
volume:9 ; year:2023 ; number:12 ; pages:e22991- |
| Links: |
|---|
| DOI / URN: |
10.1016/j.heliyon.2023.e22991 |
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| Katalog-ID: |
DOAJ098982605 |
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| 520 | |a Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural Killer cell Simultaneous ADCC and Direct Killing Assay (NK-SADKA) allows researchers to reproducibly quantify both direct killing and ADCC by human NK cells. Furthermore, this optimized experimental design allows for concurrent analysis of the NK cells via flow cytometric immunophenotyping of NK cell populations which will facilitate the identification of relationships between NK cell phenotype and the subsequent killing potential. This assay will be valuable for assessing the broader impact(s) of immunotherapy strategies on both modes of NK cell killing. | ||
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| 650 | 4 | |a ADCC | |
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| 650 | 4 | |a Immunophenotype | |
| 653 | 0 | |a Science (General) | |
| 653 | 0 | |a Social sciences (General) | |
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| 700 | 0 | |a Sean T. Hartwell |e verfasserin |4 aut | |
| 700 | 0 | |a Alexander K. Regan |e verfasserin |4 aut | |
| 700 | 0 | |a Isabelle S. Weber |e verfasserin |4 aut | |
| 700 | 0 | |a Arriana Blackmon |e verfasserin |4 aut | |
| 700 | 0 | |a Cami R. Bisson |e verfasserin |4 aut | |
| 700 | 0 | |a Angela N. Truong |e verfasserin |4 aut | |
| 700 | 0 | |a Bella A. Circo |e verfasserin |4 aut | |
| 700 | 0 | |a Jaden Nienhueser |e verfasserin |4 aut | |
| 700 | 0 | |a Donald R. Rogers |e verfasserin |4 aut | |
| 700 | 0 | |a Nathan Booher |e verfasserin |4 aut | |
| 700 | 0 | |a Nithya Rajagopalan |e verfasserin |4 aut | |
| 700 | 0 | |a Jacob W.S. Martens |e verfasserin |4 aut | |
| 700 | 0 | |a Paul W. Denton |e verfasserin |4 aut | |
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10.1016/j.heliyon.2023.e22991 doi (DE-627)DOAJ098982605 (DE-599)DOAJ52f9824e6b284dbb989cfb03dc833e9e DE-627 ger DE-627 rakwb eng Q1-390 H1-99 Maia M.C. Bennett-Boehm verfasserin aut Development and implementation of natural killer cell simultaneous ADCC and direct killing assay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural Killer cell Simultaneous ADCC and Direct Killing Assay (NK-SADKA) allows researchers to reproducibly quantify both direct killing and ADCC by human NK cells. Furthermore, this optimized experimental design allows for concurrent analysis of the NK cells via flow cytometric immunophenotyping of NK cell populations which will facilitate the identification of relationships between NK cell phenotype and the subsequent killing potential. This assay will be valuable for assessing the broader impact(s) of immunotherapy strategies on both modes of NK cell killing. NK cell Killing assay ADCC Direct killing Flow cytometry Immunophenotype Science (General) Social sciences (General) Anna R. Mahr verfasserin aut Sean T. Hartwell verfasserin aut Alexander K. Regan verfasserin aut Isabelle S. Weber verfasserin aut Arriana Blackmon verfasserin aut Cami R. Bisson verfasserin aut Angela N. Truong verfasserin aut Bella A. Circo verfasserin aut Jaden Nienhueser verfasserin aut Donald R. Rogers verfasserin aut Nathan Booher verfasserin aut Nithya Rajagopalan verfasserin aut Jacob W.S. Martens verfasserin aut Paul W. Denton verfasserin aut In Heliyon Elsevier, 2016 9(2023), 12, Seite e22991- (DE-627)835893197 (DE-600)2835763-2 24058440 nnns volume:9 year:2023 number:12 pages:e22991- https://doi.org/10.1016/j.heliyon.2023.e22991 kostenfrei https://doaj.org/article/52f9824e6b284dbb989cfb03dc833e9e kostenfrei http://www.sciencedirect.com/science/article/pii/S240584402310199X kostenfrei https://doaj.org/toc/2405-8440 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2023 12 e22991- |
| spelling |
10.1016/j.heliyon.2023.e22991 doi (DE-627)DOAJ098982605 (DE-599)DOAJ52f9824e6b284dbb989cfb03dc833e9e DE-627 ger DE-627 rakwb eng Q1-390 H1-99 Maia M.C. Bennett-Boehm verfasserin aut Development and implementation of natural killer cell simultaneous ADCC and direct killing assay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural Killer cell Simultaneous ADCC and Direct Killing Assay (NK-SADKA) allows researchers to reproducibly quantify both direct killing and ADCC by human NK cells. Furthermore, this optimized experimental design allows for concurrent analysis of the NK cells via flow cytometric immunophenotyping of NK cell populations which will facilitate the identification of relationships between NK cell phenotype and the subsequent killing potential. This assay will be valuable for assessing the broader impact(s) of immunotherapy strategies on both modes of NK cell killing. NK cell Killing assay ADCC Direct killing Flow cytometry Immunophenotype Science (General) Social sciences (General) Anna R. Mahr verfasserin aut Sean T. Hartwell verfasserin aut Alexander K. Regan verfasserin aut Isabelle S. Weber verfasserin aut Arriana Blackmon verfasserin aut Cami R. Bisson verfasserin aut Angela N. Truong verfasserin aut Bella A. Circo verfasserin aut Jaden Nienhueser verfasserin aut Donald R. Rogers verfasserin aut Nathan Booher verfasserin aut Nithya Rajagopalan verfasserin aut Jacob W.S. Martens verfasserin aut Paul W. Denton verfasserin aut In Heliyon Elsevier, 2016 9(2023), 12, Seite e22991- (DE-627)835893197 (DE-600)2835763-2 24058440 nnns volume:9 year:2023 number:12 pages:e22991- https://doi.org/10.1016/j.heliyon.2023.e22991 kostenfrei https://doaj.org/article/52f9824e6b284dbb989cfb03dc833e9e kostenfrei http://www.sciencedirect.com/science/article/pii/S240584402310199X kostenfrei https://doaj.org/toc/2405-8440 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2023 12 e22991- |
| allfields_unstemmed |
10.1016/j.heliyon.2023.e22991 doi (DE-627)DOAJ098982605 (DE-599)DOAJ52f9824e6b284dbb989cfb03dc833e9e DE-627 ger DE-627 rakwb eng Q1-390 H1-99 Maia M.C. Bennett-Boehm verfasserin aut Development and implementation of natural killer cell simultaneous ADCC and direct killing assay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural Killer cell Simultaneous ADCC and Direct Killing Assay (NK-SADKA) allows researchers to reproducibly quantify both direct killing and ADCC by human NK cells. Furthermore, this optimized experimental design allows for concurrent analysis of the NK cells via flow cytometric immunophenotyping of NK cell populations which will facilitate the identification of relationships between NK cell phenotype and the subsequent killing potential. This assay will be valuable for assessing the broader impact(s) of immunotherapy strategies on both modes of NK cell killing. NK cell Killing assay ADCC Direct killing Flow cytometry Immunophenotype Science (General) Social sciences (General) Anna R. Mahr verfasserin aut Sean T. Hartwell verfasserin aut Alexander K. Regan verfasserin aut Isabelle S. Weber verfasserin aut Arriana Blackmon verfasserin aut Cami R. Bisson verfasserin aut Angela N. Truong verfasserin aut Bella A. Circo verfasserin aut Jaden Nienhueser verfasserin aut Donald R. Rogers verfasserin aut Nathan Booher verfasserin aut Nithya Rajagopalan verfasserin aut Jacob W.S. Martens verfasserin aut Paul W. Denton verfasserin aut In Heliyon Elsevier, 2016 9(2023), 12, Seite e22991- (DE-627)835893197 (DE-600)2835763-2 24058440 nnns volume:9 year:2023 number:12 pages:e22991- https://doi.org/10.1016/j.heliyon.2023.e22991 kostenfrei https://doaj.org/article/52f9824e6b284dbb989cfb03dc833e9e kostenfrei http://www.sciencedirect.com/science/article/pii/S240584402310199X kostenfrei https://doaj.org/toc/2405-8440 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 9 2023 12 e22991- |
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Development and implementation of natural killer cell simultaneous ADCC and direct killing assay |
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Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural Killer cell Simultaneous ADCC and Direct Killing Assay (NK-SADKA) allows researchers to reproducibly quantify both direct killing and ADCC by human NK cells. Furthermore, this optimized experimental design allows for concurrent analysis of the NK cells via flow cytometric immunophenotyping of NK cell populations which will facilitate the identification of relationships between NK cell phenotype and the subsequent killing potential. This assay will be valuable for assessing the broader impact(s) of immunotherapy strategies on both modes of NK cell killing. |
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Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural Killer cell Simultaneous ADCC and Direct Killing Assay (NK-SADKA) allows researchers to reproducibly quantify both direct killing and ADCC by human NK cells. Furthermore, this optimized experimental design allows for concurrent analysis of the NK cells via flow cytometric immunophenotyping of NK cell populations which will facilitate the identification of relationships between NK cell phenotype and the subsequent killing potential. This assay will be valuable for assessing the broader impact(s) of immunotherapy strategies on both modes of NK cell killing. |
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Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural Killer cell Simultaneous ADCC and Direct Killing Assay (NK-SADKA) allows researchers to reproducibly quantify both direct killing and ADCC by human NK cells. Furthermore, this optimized experimental design allows for concurrent analysis of the NK cells via flow cytometric immunophenotyping of NK cell populations which will facilitate the identification of relationships between NK cell phenotype and the subsequent killing potential. This assay will be valuable for assessing the broader impact(s) of immunotherapy strategies on both modes of NK cell killing. |
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