CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF REDUCED AND OXIDISED ASCORBATE AND GLUTATHIONE IN ROOTS AND LEAF SEGMENTS OF Zea mays PLANTS EXPOSED TO Cd AND Cu
The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antio...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Sławomir Dresler [verfasserIn] Waldemar Maksymiec [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Acta Scientiarum Polonorum: Hortorum Cultus ; 12(2013), 6 volume:12 ; year:2013 ; number:6 |
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| 520 | |a The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root. | ||
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(DE-627)DOAJ099369524 (DE-599)DOAJ5dbb3274df194a6392c189dc2496776b DE-627 ger DE-627 rakwb eng QD415-436 SB1-1110 Sławomir Dresler verfasserin aut CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF REDUCED AND OXIDISED ASCORBATE AND GLUTATHIONE IN ROOTS AND LEAF SEGMENTS OF Zea mays PLANTS EXPOSED TO Cd AND Cu 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root. non-enzymatic antioxidants heavy metal stress plant Biochemistry Plant culture Science Q Waldemar Maksymiec verfasserin aut In Acta Scientiarum Polonorum: Hortorum Cultus 12(2013), 6 volume:12 year:2013 number:6 https://doaj.org/article/5dbb3274df194a6392c189dc2496776b kostenfrei https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2979 kostenfrei https://doaj.org/toc/1644-0692 Journal toc kostenfrei https://doaj.org/toc/2545-1405 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 12 2013 6 |
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(DE-627)DOAJ099369524 (DE-599)DOAJ5dbb3274df194a6392c189dc2496776b DE-627 ger DE-627 rakwb eng QD415-436 SB1-1110 Sławomir Dresler verfasserin aut CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF REDUCED AND OXIDISED ASCORBATE AND GLUTATHIONE IN ROOTS AND LEAF SEGMENTS OF Zea mays PLANTS EXPOSED TO Cd AND Cu 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root. non-enzymatic antioxidants heavy metal stress plant Biochemistry Plant culture Science Q Waldemar Maksymiec verfasserin aut In Acta Scientiarum Polonorum: Hortorum Cultus 12(2013), 6 volume:12 year:2013 number:6 https://doaj.org/article/5dbb3274df194a6392c189dc2496776b kostenfrei https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2979 kostenfrei https://doaj.org/toc/1644-0692 Journal toc kostenfrei https://doaj.org/toc/2545-1405 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 12 2013 6 |
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CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF REDUCED AND OXIDISED ASCORBATE AND GLUTATHIONE IN ROOTS AND LEAF SEGMENTS OF Zea mays PLANTS EXPOSED TO Cd AND Cu |
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capillary zone electrophoresis for determination of reduced and oxidised ascorbate and glutathione in roots and leaf segments of zea mays plants exposed to cd and cu |
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CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF REDUCED AND OXIDISED ASCORBATE AND GLUTATHIONE IN ROOTS AND LEAF SEGMENTS OF Zea mays PLANTS EXPOSED TO Cd AND Cu |
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The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root. |
| abstractGer |
The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root. |
| abstract_unstemmed |
The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root. |
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CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF REDUCED AND OXIDISED ASCORBATE AND GLUTATHIONE IN ROOTS AND LEAF SEGMENTS OF Zea mays PLANTS EXPOSED TO Cd AND Cu |
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https://doaj.org/article/5dbb3274df194a6392c189dc2496776b https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2979 https://doaj.org/toc/1644-0692 https://doaj.org/toc/2545-1405 |
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Waldemar Maksymiec |
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2024-07-03T22:26:27.726Z |
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