<i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration

Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with sk...
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Autor*in:	Mariko Yamamoto Kawai [verfasserIn] Takeshi Yoshida [verfasserIn] Tomoki Kato [verfasserIn] Takuma Watanabe [verfasserIn] Marina Kashiwagi [verfasserIn] Shigeki Yamanaka [verfasserIn] Hiromitsu Yamamoto [verfasserIn] Shigeki Nagahiro [verfasserIn] Tsutomu Iwamoto [verfasserIn] Khan Masud [verfasserIn] Kazuhiro Aoki [verfasserIn] Kiyoshi Ohura [verfasserIn] Kazumasa Nakao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	muscle bone

Übergeordnetes Werk:	In: Materials - MDPI AG, 2009, 17(2024), 4, p 880
Übergeordnetes Werk:	volume:17 ; year:2024 ; number:4, p 880

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ma17040880

Katalog-ID:	DOAJ099615711

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520			\|a Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects.
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allfields	10.3390/ma17040880 doi (DE-627)DOAJ099615711 (DE-599)DOAJ5ed40113c6a24024a3a1ae49df5a5a91 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Mariko Yamamoto Kawai verfasserin aut <i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects. muscle bone <i<bmp-2</i< Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Takeshi Yoshida verfasserin aut Tomoki Kato verfasserin aut Takuma Watanabe verfasserin aut Marina Kashiwagi verfasserin aut Shigeki Yamanaka verfasserin aut Hiromitsu Yamamoto verfasserin aut Shigeki Nagahiro verfasserin aut Tsutomu Iwamoto verfasserin aut Khan Masud verfasserin aut Kazuhiro Aoki verfasserin aut Kiyoshi Ohura verfasserin aut Kazumasa Nakao verfasserin aut In Materials MDPI AG, 2009 17(2024), 4, p 880 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:17 year:2024 number:4, p 880 https://doi.org/10.3390/ma17040880 kostenfrei https://doaj.org/article/5ed40113c6a24024a3a1ae49df5a5a91 kostenfrei https://www.mdpi.com/1996-1944/17/4/880 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 4, p 880
spelling	10.3390/ma17040880 doi (DE-627)DOAJ099615711 (DE-599)DOAJ5ed40113c6a24024a3a1ae49df5a5a91 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Mariko Yamamoto Kawai verfasserin aut <i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects. muscle bone <i<bmp-2</i< Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Takeshi Yoshida verfasserin aut Tomoki Kato verfasserin aut Takuma Watanabe verfasserin aut Marina Kashiwagi verfasserin aut Shigeki Yamanaka verfasserin aut Hiromitsu Yamamoto verfasserin aut Shigeki Nagahiro verfasserin aut Tsutomu Iwamoto verfasserin aut Khan Masud verfasserin aut Kazuhiro Aoki verfasserin aut Kiyoshi Ohura verfasserin aut Kazumasa Nakao verfasserin aut In Materials MDPI AG, 2009 17(2024), 4, p 880 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:17 year:2024 number:4, p 880 https://doi.org/10.3390/ma17040880 kostenfrei https://doaj.org/article/5ed40113c6a24024a3a1ae49df5a5a91 kostenfrei https://www.mdpi.com/1996-1944/17/4/880 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 4, p 880
allfields_unstemmed	10.3390/ma17040880 doi (DE-627)DOAJ099615711 (DE-599)DOAJ5ed40113c6a24024a3a1ae49df5a5a91 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Mariko Yamamoto Kawai verfasserin aut <i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects. muscle bone <i<bmp-2</i< Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Takeshi Yoshida verfasserin aut Tomoki Kato verfasserin aut Takuma Watanabe verfasserin aut Marina Kashiwagi verfasserin aut Shigeki Yamanaka verfasserin aut Hiromitsu Yamamoto verfasserin aut Shigeki Nagahiro verfasserin aut Tsutomu Iwamoto verfasserin aut Khan Masud verfasserin aut Kazuhiro Aoki verfasserin aut Kiyoshi Ohura verfasserin aut Kazumasa Nakao verfasserin aut In Materials MDPI AG, 2009 17(2024), 4, p 880 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:17 year:2024 number:4, p 880 https://doi.org/10.3390/ma17040880 kostenfrei https://doaj.org/article/5ed40113c6a24024a3a1ae49df5a5a91 kostenfrei https://www.mdpi.com/1996-1944/17/4/880 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 4, p 880
allfieldsGer	10.3390/ma17040880 doi (DE-627)DOAJ099615711 (DE-599)DOAJ5ed40113c6a24024a3a1ae49df5a5a91 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Mariko Yamamoto Kawai verfasserin aut <i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects. muscle bone <i<bmp-2</i< Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Takeshi Yoshida verfasserin aut Tomoki Kato verfasserin aut Takuma Watanabe verfasserin aut Marina Kashiwagi verfasserin aut Shigeki Yamanaka verfasserin aut Hiromitsu Yamamoto verfasserin aut Shigeki Nagahiro verfasserin aut Tsutomu Iwamoto verfasserin aut Khan Masud verfasserin aut Kazuhiro Aoki verfasserin aut Kiyoshi Ohura verfasserin aut Kazumasa Nakao verfasserin aut In Materials MDPI AG, 2009 17(2024), 4, p 880 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:17 year:2024 number:4, p 880 https://doi.org/10.3390/ma17040880 kostenfrei https://doaj.org/article/5ed40113c6a24024a3a1ae49df5a5a91 kostenfrei https://www.mdpi.com/1996-1944/17/4/880 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 4, p 880
allfieldsSound	10.3390/ma17040880 doi (DE-627)DOAJ099615711 (DE-599)DOAJ5ed40113c6a24024a3a1ae49df5a5a91 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Mariko Yamamoto Kawai verfasserin aut <i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects. muscle bone <i<bmp-2</i< Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Takeshi Yoshida verfasserin aut Tomoki Kato verfasserin aut Takuma Watanabe verfasserin aut Marina Kashiwagi verfasserin aut Shigeki Yamanaka verfasserin aut Hiromitsu Yamamoto verfasserin aut Shigeki Nagahiro verfasserin aut Tsutomu Iwamoto verfasserin aut Khan Masud verfasserin aut Kazuhiro Aoki verfasserin aut Kiyoshi Ohura verfasserin aut Kazumasa Nakao verfasserin aut In Materials MDPI AG, 2009 17(2024), 4, p 880 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:17 year:2024 number:4, p 880 https://doi.org/10.3390/ma17040880 kostenfrei https://doaj.org/article/5ed40113c6a24024a3a1ae49df5a5a91 kostenfrei https://www.mdpi.com/1996-1944/17/4/880 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 4, p 880
language	English
source	In Materials 17(2024), 4, p 880 volume:17 year:2024 number:4, p 880
sourceStr	In Materials 17(2024), 4, p 880 volume:17 year:2024 number:4, p 880
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	muscle bone <i<bmp-2</i< Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics
isfreeaccess_bool	true
container_title	Materials
authorswithroles_txt_mv	Mariko Yamamoto Kawai @@aut@@ Takeshi Yoshida @@aut@@ Tomoki Kato @@aut@@ Takuma Watanabe @@aut@@ Marina Kashiwagi @@aut@@ Shigeki Yamanaka @@aut@@ Hiromitsu Yamamoto @@aut@@ Shigeki Nagahiro @@aut@@ Tsutomu Iwamoto @@aut@@ Khan Masud @@aut@@ Kazuhiro Aoki @@aut@@ Kiyoshi Ohura @@aut@@ Kazumasa Nakao @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	595712649
id	DOAJ099615711
language_de	englisch
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callnumber-first	T - Technology
author	Mariko Yamamoto Kawai
spellingShingle	Mariko Yamamoto Kawai misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc muscle misc bone misc <i<bmp-2</i< misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics <i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration
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topic_title	TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 <i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration muscle bone <i<bmp-2</i<
topic	misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc muscle misc bone misc <i<bmp-2</i< misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics
topic_unstemmed	misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc muscle misc bone misc <i<bmp-2</i< misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics
topic_browse	misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc muscle misc bone misc <i<bmp-2</i< misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics
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title	<i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration
ctrlnum	(DE-627)DOAJ099615711 (DE-599)DOAJ5ed40113c6a24024a3a1ae49df5a5a91
title_full	<i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration
author_sort	Mariko Yamamoto Kawai
journal	Materials
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author_browse	Mariko Yamamoto Kawai Takeshi Yoshida Tomoki Kato Takuma Watanabe Marina Kashiwagi Shigeki Yamanaka Hiromitsu Yamamoto Shigeki Nagahiro Tsutomu Iwamoto Khan Masud Kazuhiro Aoki Kiyoshi Ohura Kazumasa Nakao
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author-letter	Mariko Yamamoto Kawai
doi_str_mv	10.3390/ma17040880
author2-role	verfasserin
title_sort	<i<bmp-2</i< gene-transferred skeletal muscles with needle-type electrodes as efficient and reliable biomaterials for bone regeneration
callnumber	TK1-9971
title_auth	<i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration
abstract	Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects.
abstractGer	Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects.
abstract_unstemmed	Background: Bone morphogenetic protein-2 (<i<bmp-2</i<) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the <i<bmp-2</i< gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral <i<bmp-2</i< gene expression plasmid vector (pCAGGS-<i<bmp-2</i<) and needle-type electrodes. Methods: We injected the <i<bmp-2</i< gene with pCAGGS-<i<bmp-2</i< into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the <i<bmp-2</i< gene to skeletal muscles, making them potential biomaterials for repairing bone defects.
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title_short	<i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration
url	https://doi.org/10.3390/ma17040880 https://doaj.org/article/5ed40113c6a24024a3a1ae49df5a5a91 https://www.mdpi.com/1996-1944/17/4/880 https://doaj.org/toc/1996-1944
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author2Str	Takeshi Yoshida Tomoki Kato Takuma Watanabe Marina Kashiwagi Shigeki Yamanaka Hiromitsu Yamamoto Shigeki Nagahiro Tsutomu Iwamoto Khan Masud Kazuhiro Aoki Kiyoshi Ohura Kazumasa Nakao
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up_date	2024-07-03T23:36:43.742Z
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<i<bmp-2</i< Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration

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