Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i<

Lithium chloride (LiCl) has been used in signalling and molecular studies of animals, plants, and yeast. However, information on its roles in basidiomycetous fungi is still limited. In this study, we used RNA-Seq to study the effects of LiCl on <i<Coprinopsis cinerea</i<. LiCl enhanced m...
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Autor*in:	Po-Lam Chan [verfasserIn] Hoi-Shan Kwan [verfasserIn] Yichun Xie [verfasserIn] Ka-Hing Wong [verfasserIn] Jinhui Chang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	glycogen synthase kinase3 fruiting body development environmental signals kinases RNA-Seq

Übergeordnetes Werk:	In: Journal of Fungi - MDPI AG, 2015, 10(2024), 2, p 140
Übergeordnetes Werk:	volume:10 ; year:2024 ; number:2, p 140

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/jof10020140

Katalog-ID:	DOAJ099622661

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520			\|a Lithium chloride (LiCl) has been used in signalling and molecular studies of animals, plants, and yeast. However, information on its roles in basidiomycetous fungi is still limited. In this study, we used RNA-Seq to study the effects of LiCl on <i<Coprinopsis cinerea</i<. LiCl enhanced mycelial growth and inhibited fruiting body formation in <i<C. cinerea</i<. RNA-Seq of the LiCl-treated <i<C. cinerea</i< resulted in a total of 14,128 genes. There were 1199 differentially expressed genes (DEGs) between the LiCl-treated samples and control samples in the mycelium stage (the first time point), and 1391 DEGs were detected when the control samples were forming hyphal knots while the treated samples were still in the mycelium (the second time point). Pathway enrichment analysis of the DEGs revealed a significant association between enhanced mycelium growth in the LiCl-treated <i<C. cinerea</i< and metabolic pathways. In addition, the DEGs involved in cellular process pathways, including “cell cycle-yeast” and “meiosis-yeast”, were identified in suppressed <i<C. cinerea</i< fruiting body formation by LiCl under favourable environmental conditions. As LiCl can predominantly inhibit the activity of glycogen synthase kinase3 (GSK3), our findings suggest that LiCl affects the expression of genes involved in fruiting body initiation and cellular processes by inhibiting GSK3 activity which is essential for fruiting body formation.
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spelling	10.3390/jof10020140 doi (DE-627)DOAJ099622661 (DE-599)DOAJd9016842db924b33a93152832c1f92bd DE-627 ger DE-627 rakwb eng QH301-705.5 Po-Lam Chan verfasserin aut Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i< 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lithium chloride (LiCl) has been used in signalling and molecular studies of animals, plants, and yeast. However, information on its roles in basidiomycetous fungi is still limited. In this study, we used RNA-Seq to study the effects of LiCl on <i<Coprinopsis cinerea</i<. LiCl enhanced mycelial growth and inhibited fruiting body formation in <i<C. cinerea</i<. RNA-Seq of the LiCl-treated <i<C. cinerea</i< resulted in a total of 14,128 genes. There were 1199 differentially expressed genes (DEGs) between the LiCl-treated samples and control samples in the mycelium stage (the first time point), and 1391 DEGs were detected when the control samples were forming hyphal knots while the treated samples were still in the mycelium (the second time point). Pathway enrichment analysis of the DEGs revealed a significant association between enhanced mycelium growth in the LiCl-treated <i<C. cinerea</i< and metabolic pathways. In addition, the DEGs involved in cellular process pathways, including “cell cycle-yeast” and “meiosis-yeast”, were identified in suppressed <i<C. cinerea</i< fruiting body formation by LiCl under favourable environmental conditions. As LiCl can predominantly inhibit the activity of glycogen synthase kinase3 (GSK3), our findings suggest that LiCl affects the expression of genes involved in fruiting body initiation and cellular processes by inhibiting GSK3 activity which is essential for fruiting body formation. glycogen synthase kinase3 fruiting body development environmental signals kinases RNA-Seq Biology (General) Hoi-Shan Kwan verfasserin aut Yichun Xie verfasserin aut Ka-Hing Wong verfasserin aut Jinhui Chang verfasserin aut In Journal of Fungi MDPI AG, 2015 10(2024), 2, p 140 (DE-627)796588538 (DE-600)2784229-0 2309608X nnns volume:10 year:2024 number:2, p 140 https://doi.org/10.3390/jof10020140 kostenfrei https://doaj.org/article/d9016842db924b33a93152832c1f92bd kostenfrei https://www.mdpi.com/2309-608X/10/2/140 kostenfrei https://doaj.org/toc/2309-608X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2024 2, p 140
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authorswithroles_txt_mv	Po-Lam Chan @@aut@@ Hoi-Shan Kwan @@aut@@ Yichun Xie @@aut@@ Ka-Hing Wong @@aut@@ Jinhui Chang @@aut@@
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callnumber-first	Q - Science
author	Po-Lam Chan
spellingShingle	Po-Lam Chan misc QH301-705.5 misc glycogen synthase kinase3 misc fruiting body development misc environmental signals misc kinases misc RNA-Seq misc Biology (General) Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i<
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topic_title	QH301-705.5 Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i< glycogen synthase kinase3 fruiting body development environmental signals kinases RNA-Seq
topic	misc QH301-705.5 misc glycogen synthase kinase3 misc fruiting body development misc environmental signals misc kinases misc RNA-Seq misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc glycogen synthase kinase3 misc fruiting body development misc environmental signals misc kinases misc RNA-Seq misc Biology (General)
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title	Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i<
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title_full	Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i<
author_sort	Po-Lam Chan
journal	Journal of Fungi
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author_browse	Po-Lam Chan Hoi-Shan Kwan Yichun Xie Ka-Hing Wong Jinhui Chang
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title_sort	transcriptome analysis reveals mycelial and fruiting responses to lithium chloride in <i<coprinopsis cinerea</i<
callnumber	QH301-705.5
title_auth	Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i<
abstract	Lithium chloride (LiCl) has been used in signalling and molecular studies of animals, plants, and yeast. However, information on its roles in basidiomycetous fungi is still limited. In this study, we used RNA-Seq to study the effects of LiCl on <i<Coprinopsis cinerea</i<. LiCl enhanced mycelial growth and inhibited fruiting body formation in <i<C. cinerea</i<. RNA-Seq of the LiCl-treated <i<C. cinerea</i< resulted in a total of 14,128 genes. There were 1199 differentially expressed genes (DEGs) between the LiCl-treated samples and control samples in the mycelium stage (the first time point), and 1391 DEGs were detected when the control samples were forming hyphal knots while the treated samples were still in the mycelium (the second time point). Pathway enrichment analysis of the DEGs revealed a significant association between enhanced mycelium growth in the LiCl-treated <i<C. cinerea</i< and metabolic pathways. In addition, the DEGs involved in cellular process pathways, including “cell cycle-yeast” and “meiosis-yeast”, were identified in suppressed <i<C. cinerea</i< fruiting body formation by LiCl under favourable environmental conditions. As LiCl can predominantly inhibit the activity of glycogen synthase kinase3 (GSK3), our findings suggest that LiCl affects the expression of genes involved in fruiting body initiation and cellular processes by inhibiting GSK3 activity which is essential for fruiting body formation.
abstractGer	Lithium chloride (LiCl) has been used in signalling and molecular studies of animals, plants, and yeast. However, information on its roles in basidiomycetous fungi is still limited. In this study, we used RNA-Seq to study the effects of LiCl on <i<Coprinopsis cinerea</i<. LiCl enhanced mycelial growth and inhibited fruiting body formation in <i<C. cinerea</i<. RNA-Seq of the LiCl-treated <i<C. cinerea</i< resulted in a total of 14,128 genes. There were 1199 differentially expressed genes (DEGs) between the LiCl-treated samples and control samples in the mycelium stage (the first time point), and 1391 DEGs were detected when the control samples were forming hyphal knots while the treated samples were still in the mycelium (the second time point). Pathway enrichment analysis of the DEGs revealed a significant association between enhanced mycelium growth in the LiCl-treated <i<C. cinerea</i< and metabolic pathways. In addition, the DEGs involved in cellular process pathways, including “cell cycle-yeast” and “meiosis-yeast”, were identified in suppressed <i<C. cinerea</i< fruiting body formation by LiCl under favourable environmental conditions. As LiCl can predominantly inhibit the activity of glycogen synthase kinase3 (GSK3), our findings suggest that LiCl affects the expression of genes involved in fruiting body initiation and cellular processes by inhibiting GSK3 activity which is essential for fruiting body formation.
abstract_unstemmed	Lithium chloride (LiCl) has been used in signalling and molecular studies of animals, plants, and yeast. However, information on its roles in basidiomycetous fungi is still limited. In this study, we used RNA-Seq to study the effects of LiCl on <i<Coprinopsis cinerea</i<. LiCl enhanced mycelial growth and inhibited fruiting body formation in <i<C. cinerea</i<. RNA-Seq of the LiCl-treated <i<C. cinerea</i< resulted in a total of 14,128 genes. There were 1199 differentially expressed genes (DEGs) between the LiCl-treated samples and control samples in the mycelium stage (the first time point), and 1391 DEGs were detected when the control samples were forming hyphal knots while the treated samples were still in the mycelium (the second time point). Pathway enrichment analysis of the DEGs revealed a significant association between enhanced mycelium growth in the LiCl-treated <i<C. cinerea</i< and metabolic pathways. In addition, the DEGs involved in cellular process pathways, including “cell cycle-yeast” and “meiosis-yeast”, were identified in suppressed <i<C. cinerea</i< fruiting body formation by LiCl under favourable environmental conditions. As LiCl can predominantly inhibit the activity of glycogen synthase kinase3 (GSK3), our findings suggest that LiCl affects the expression of genes involved in fruiting body initiation and cellular processes by inhibiting GSK3 activity which is essential for fruiting body formation.
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container_issue	2, p 140
title_short	Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride in <i<Coprinopsis cinerea</i<
url	https://doi.org/10.3390/jof10020140 https://doaj.org/article/d9016842db924b33a93152832c1f92bd https://www.mdpi.com/2309-608X/10/2/140 https://doaj.org/toc/2309-608X
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up_date	2024-07-03T23:38:45.214Z
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