The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells

Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk a...
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Autor*in:	Mikhail G. Akimov [verfasserIn] Natalia M. Gretskaya [verfasserIn] Evgenia I. Gorbacheva [verfasserIn] Nisreen Khadour [verfasserIn] Valeria S. Chernavskaya [verfasserIn] Galina D. Sherstyanykh [verfasserIn] Tatiana F. Kovaleko [verfasserIn] Elena V. Fomina-Ageeva [verfasserIn] Vladimir V. Bezuglov [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	CB1 CB2 GPR18 TRPV1 LPI anandamide

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 25(2024), 4, p 2271
Übergeordnetes Werk:	volume:25 ; year:2024 ; number:4, p 2271

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms25042271

Katalog-ID:	DOAJ09963287X

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allfields	10.3390/ijms25042271 doi (DE-627)DOAJ09963287X (DE-599)DOAJ32dd6e16e28345efafdacaf90239eec2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mikhail G. Akimov verfasserin aut The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages. CB1 CB2 GPR18 TRPV1 LPI anandamide Biology (General) Chemistry Natalia M. Gretskaya verfasserin aut Evgenia I. Gorbacheva verfasserin aut Nisreen Khadour verfasserin aut Valeria S. Chernavskaya verfasserin aut Galina D. Sherstyanykh verfasserin aut Tatiana F. Kovaleko verfasserin aut Elena V. Fomina-Ageeva verfasserin aut Vladimir V. Bezuglov verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2271 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2271 https://doi.org/10.3390/ijms25042271 kostenfrei https://doaj.org/article/32dd6e16e28345efafdacaf90239eec2 kostenfrei https://www.mdpi.com/1422-0067/25/4/2271 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2271
spelling	10.3390/ijms25042271 doi (DE-627)DOAJ09963287X (DE-599)DOAJ32dd6e16e28345efafdacaf90239eec2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mikhail G. Akimov verfasserin aut The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages. CB1 CB2 GPR18 TRPV1 LPI anandamide Biology (General) Chemistry Natalia M. Gretskaya verfasserin aut Evgenia I. Gorbacheva verfasserin aut Nisreen Khadour verfasserin aut Valeria S. Chernavskaya verfasserin aut Galina D. Sherstyanykh verfasserin aut Tatiana F. Kovaleko verfasserin aut Elena V. Fomina-Ageeva verfasserin aut Vladimir V. Bezuglov verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2271 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2271 https://doi.org/10.3390/ijms25042271 kostenfrei https://doaj.org/article/32dd6e16e28345efafdacaf90239eec2 kostenfrei https://www.mdpi.com/1422-0067/25/4/2271 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2271
allfields_unstemmed	10.3390/ijms25042271 doi (DE-627)DOAJ09963287X (DE-599)DOAJ32dd6e16e28345efafdacaf90239eec2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mikhail G. Akimov verfasserin aut The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages. CB1 CB2 GPR18 TRPV1 LPI anandamide Biology (General) Chemistry Natalia M. Gretskaya verfasserin aut Evgenia I. Gorbacheva verfasserin aut Nisreen Khadour verfasserin aut Valeria S. Chernavskaya verfasserin aut Galina D. Sherstyanykh verfasserin aut Tatiana F. Kovaleko verfasserin aut Elena V. Fomina-Ageeva verfasserin aut Vladimir V. Bezuglov verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2271 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2271 https://doi.org/10.3390/ijms25042271 kostenfrei https://doaj.org/article/32dd6e16e28345efafdacaf90239eec2 kostenfrei https://www.mdpi.com/1422-0067/25/4/2271 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2271
allfieldsGer	10.3390/ijms25042271 doi (DE-627)DOAJ09963287X (DE-599)DOAJ32dd6e16e28345efafdacaf90239eec2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mikhail G. Akimov verfasserin aut The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages. CB1 CB2 GPR18 TRPV1 LPI anandamide Biology (General) Chemistry Natalia M. Gretskaya verfasserin aut Evgenia I. Gorbacheva verfasserin aut Nisreen Khadour verfasserin aut Valeria S. Chernavskaya verfasserin aut Galina D. Sherstyanykh verfasserin aut Tatiana F. Kovaleko verfasserin aut Elena V. Fomina-Ageeva verfasserin aut Vladimir V. Bezuglov verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2271 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2271 https://doi.org/10.3390/ijms25042271 kostenfrei https://doaj.org/article/32dd6e16e28345efafdacaf90239eec2 kostenfrei https://www.mdpi.com/1422-0067/25/4/2271 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2271
allfieldsSound	10.3390/ijms25042271 doi (DE-627)DOAJ09963287X (DE-599)DOAJ32dd6e16e28345efafdacaf90239eec2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mikhail G. Akimov verfasserin aut The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages. CB1 CB2 GPR18 TRPV1 LPI anandamide Biology (General) Chemistry Natalia M. Gretskaya verfasserin aut Evgenia I. Gorbacheva verfasserin aut Nisreen Khadour verfasserin aut Valeria S. Chernavskaya verfasserin aut Galina D. Sherstyanykh verfasserin aut Tatiana F. Kovaleko verfasserin aut Elena V. Fomina-Ageeva verfasserin aut Vladimir V. Bezuglov verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2271 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2271 https://doi.org/10.3390/ijms25042271 kostenfrei https://doaj.org/article/32dd6e16e28345efafdacaf90239eec2 kostenfrei https://www.mdpi.com/1422-0067/25/4/2271 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2271
language	English
source	In International Journal of Molecular Sciences 25(2024), 4, p 2271 volume:25 year:2024 number:4, p 2271
sourceStr	In International Journal of Molecular Sciences 25(2024), 4, p 2271 volume:25 year:2024 number:4, p 2271
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CB1 CB2 GPR18 TRPV1 LPI anandamide Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Mikhail G. Akimov @@aut@@ Natalia M. Gretskaya @@aut@@ Evgenia I. Gorbacheva @@aut@@ Nisreen Khadour @@aut@@ Valeria S. Chernavskaya @@aut@@ Galina D. Sherstyanykh @@aut@@ Tatiana F. Kovaleko @@aut@@ Elena V. Fomina-Ageeva @@aut@@ Vladimir V. Bezuglov @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	316340715
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language_de	englisch
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callnumber-first	Q - Science
author	Mikhail G. Akimov
spellingShingle	Mikhail G. Akimov misc QH301-705.5 misc QD1-999 misc CB1 misc CB2 misc GPR18 misc TRPV1 misc LPI misc anandamide misc Biology (General) misc Chemistry The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells
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topic_title	QH301-705.5 QD1-999 The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells CB1 CB2 GPR18 TRPV1 LPI anandamide
topic	misc QH301-705.5 misc QD1-999 misc CB1 misc CB2 misc GPR18 misc TRPV1 misc LPI misc anandamide misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc CB1 misc CB2 misc GPR18 misc TRPV1 misc LPI misc anandamide misc Biology (General) misc Chemistry
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title	The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells
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title_full	The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells
author_sort	Mikhail G. Akimov
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author_browse	Mikhail G. Akimov Natalia M. Gretskaya Evgenia I. Gorbacheva Nisreen Khadour Valeria S. Chernavskaya Galina D. Sherstyanykh Tatiana F. Kovaleko Elena V. Fomina-Ageeva Vladimir V. Bezuglov
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author-letter	Mikhail G. Akimov
doi_str_mv	10.3390/ijms25042271
author2-role	verfasserin
title_sort	interaction of the endocannabinoid anandamide and paracannabinoid lysophosphatidylinositol during cell death induction in human breast cancer cells
callnumber	QH301-705.5
title_auth	The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells
abstract	Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages.
abstractGer	Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages.
abstract_unstemmed	Endocannabinoid anandamide (AEA) and paracannabinoid lysophosphatidylinositol (LPI) play a significant role in cancer cell proliferation regulation. While anandamide inhibits the proliferation of cancer cells, LPI is known as a cancer stimulant. Despite the known endocannabinoid receptor crosstalk and simultaneous presence in the cancer microenvironment of both molecules, their combined activity has never been studied. We evaluated the effect of LPI on the AEA activity in six human breast cancer cell lines of different carcinogenicity (MCF-10A, MCF-7, BT-474, BT-20, SK-BR-3, MDA-MB-231) using resazurin and LDH tests after a 72 h incubation. AEA exerted both anti-proliferative and cytotoxic activity with EC<sub<50</sub< in the range from 31 to 80 µM. LPI did not significantly affect the cell viability. Depending on the cell line, the response to the LPI–AEA combination varied from a decrease in AEA cytotoxicity to an increase in it. Based on the inhibitor analysis of the endocannabinoid receptor panel, we showed that for the former effect, an active GPR18 receptor was required and for the latter, an active CB2 receptor. The data obtained for the first time are important for the understanding the manner by which endocannabinoid receptor ligands acting simultaneously can modulate cancer growth at different stages.
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container_issue	4, p 2271
title_short	The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells
url	https://doi.org/10.3390/ijms25042271 https://doaj.org/article/32dd6e16e28345efafdacaf90239eec2 https://www.mdpi.com/1422-0067/25/4/2271 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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up_date	2024-07-03T23:41:42.816Z
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The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells

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