Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<)

The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Mingjian Liu [verfasserIn] Hui Ding [verfasserIn] Chaofan Jin [verfasserIn] Mingyi Wang [verfasserIn] Peiyu Li [verfasserIn] Zhenmin Bao [verfasserIn] Bo Wang [verfasserIn] Jingjie Hu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 25(2024), 4, p 2180
Übergeordnetes Werk:	volume:25 ; year:2024 ; number:4, p 2180

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms25042180

Katalog-ID:	DOAJ099633809

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allfields	10.3390/ijms25042180 doi (DE-627)DOAJ099633809 (DE-599)DOAJ569e2e4c65a940b79f30c9562473fb69 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mingjian Liu verfasserin aut Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding. <i<Plectropomus leopardus</i< 17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development Biology (General) Chemistry Hui Ding verfasserin aut Chaofan Jin verfasserin aut Mingyi Wang verfasserin aut Peiyu Li verfasserin aut Zhenmin Bao verfasserin aut Bo Wang verfasserin aut Jingjie Hu verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2180 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2180 https://doi.org/10.3390/ijms25042180 kostenfrei https://doaj.org/article/569e2e4c65a940b79f30c9562473fb69 kostenfrei https://www.mdpi.com/1422-0067/25/4/2180 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2180
spelling	10.3390/ijms25042180 doi (DE-627)DOAJ099633809 (DE-599)DOAJ569e2e4c65a940b79f30c9562473fb69 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mingjian Liu verfasserin aut Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding. <i<Plectropomus leopardus</i< 17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development Biology (General) Chemistry Hui Ding verfasserin aut Chaofan Jin verfasserin aut Mingyi Wang verfasserin aut Peiyu Li verfasserin aut Zhenmin Bao verfasserin aut Bo Wang verfasserin aut Jingjie Hu verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2180 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2180 https://doi.org/10.3390/ijms25042180 kostenfrei https://doaj.org/article/569e2e4c65a940b79f30c9562473fb69 kostenfrei https://www.mdpi.com/1422-0067/25/4/2180 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2180
allfields_unstemmed	10.3390/ijms25042180 doi (DE-627)DOAJ099633809 (DE-599)DOAJ569e2e4c65a940b79f30c9562473fb69 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mingjian Liu verfasserin aut Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding. <i<Plectropomus leopardus</i< 17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development Biology (General) Chemistry Hui Ding verfasserin aut Chaofan Jin verfasserin aut Mingyi Wang verfasserin aut Peiyu Li verfasserin aut Zhenmin Bao verfasserin aut Bo Wang verfasserin aut Jingjie Hu verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2180 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2180 https://doi.org/10.3390/ijms25042180 kostenfrei https://doaj.org/article/569e2e4c65a940b79f30c9562473fb69 kostenfrei https://www.mdpi.com/1422-0067/25/4/2180 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2180
allfieldsGer	10.3390/ijms25042180 doi (DE-627)DOAJ099633809 (DE-599)DOAJ569e2e4c65a940b79f30c9562473fb69 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mingjian Liu verfasserin aut Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding. <i<Plectropomus leopardus</i< 17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development Biology (General) Chemistry Hui Ding verfasserin aut Chaofan Jin verfasserin aut Mingyi Wang verfasserin aut Peiyu Li verfasserin aut Zhenmin Bao verfasserin aut Bo Wang verfasserin aut Jingjie Hu verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2180 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2180 https://doi.org/10.3390/ijms25042180 kostenfrei https://doaj.org/article/569e2e4c65a940b79f30c9562473fb69 kostenfrei https://www.mdpi.com/1422-0067/25/4/2180 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2180
allfieldsSound	10.3390/ijms25042180 doi (DE-627)DOAJ099633809 (DE-599)DOAJ569e2e4c65a940b79f30c9562473fb69 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Mingjian Liu verfasserin aut Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<) 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding. <i<Plectropomus leopardus</i< 17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development Biology (General) Chemistry Hui Ding verfasserin aut Chaofan Jin verfasserin aut Mingyi Wang verfasserin aut Peiyu Li verfasserin aut Zhenmin Bao verfasserin aut Bo Wang verfasserin aut Jingjie Hu verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2180 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2180 https://doi.org/10.3390/ijms25042180 kostenfrei https://doaj.org/article/569e2e4c65a940b79f30c9562473fb69 kostenfrei https://www.mdpi.com/1422-0067/25/4/2180 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2180
language	English
source	In International Journal of Molecular Sciences 25(2024), 4, p 2180 volume:25 year:2024 number:4, p 2180
sourceStr	In International Journal of Molecular Sciences 25(2024), 4, p 2180 volume:25 year:2024 number:4, p 2180
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Plectropomus leopardus</i< 17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Mingjian Liu @@aut@@ Hui Ding @@aut@@ Chaofan Jin @@aut@@ Mingyi Wang @@aut@@ Peiyu Li @@aut@@ Zhenmin Bao @@aut@@ Bo Wang @@aut@@ Jingjie Hu @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
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callnumber-first	Q - Science
author	Mingjian Liu
spellingShingle	Mingjian Liu misc QH301-705.5 misc QD1-999 misc <i<Plectropomus leopardus</i< misc 17β-Hydroxysteroid Dehydrogenase misc sex differentiation misc steroidogenesis misc gonadal development misc Biology (General) misc Chemistry Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
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topic_title	QH301-705.5 QD1-999 Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<) <i<Plectropomus leopardus</i< 17β-Hydroxysteroid Dehydrogenase sex differentiation steroidogenesis gonadal development
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title	Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
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title_full	Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
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author_browse	Mingjian Liu Hui Ding Chaofan Jin Mingyi Wang Peiyu Li Zhenmin Bao Bo Wang Jingjie Hu
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title_sort	theoretical analysis and expression profiling of 17β-hydroxysteroid dehydrogenase genes in gonadal development and steroidogenesis of leopard coral grouper (<i<plectropomus leopardus</i<)
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title_auth	Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
abstract	The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding.
abstractGer	The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding.
abstract_unstemmed	The differentiation and developmental trajectory of fish gonads, significantly important for fish breeding, culture, and production, has long been a focal point in the fields of fish genetics and developmental biology. However, the mechanism of gonadal differentiation in leopard coral grouper (<i<Plectropomus leopardus</i<) remains unclear. This study investigates the 17β-Hydroxysteroid Dehydrogenase (Hsd17b) gene family in <i<P. leopardus</i<, with a focus on gene characterization, expression profiling, and functional analysis. The results reveal that the <i<P. leopardus</i<’s Hsd17b gene family comprises 11 members, all belonging to the SDR superfamily. The amino acid similarity is only 12.96%, but conserved motifs, such as TGxxxGxG and S-Y-K, are present in these genes. <i<Hsd17b12a</i< and <i<Hsd17b12b</i< are unique homologs in fish, and chromosomal localization has confirmed that they are not derived from different transcripts of the same gene, but rather are two independent genes. The Hsd17b family genes, predominantly expressed in the liver, heart, gills, kidneys, and gonads, are involved in synthesizing or metabolizing sex steroid hormones and neurotransmitters, with their expression patterns during gonadal development categorized into three distinct categories. Notably, <i<Hsd17b4</i< and <i<Hsd17b12a</i< were highly expressed in the testis and ovary, respectively, suggesting their involvement in the development of reproductive cells in these organs. Fluorescence in situ hybridization (FISH) further indicated specific expression sites for these genes, with <i<Hsd17b4</i< primarily expressed in germ stem cells and <i<Hsd17b12a</i< in oocytes. This comprehensive study provides foundational insights into the role of the Hsd17b gene family in gonadal development and steroidogenesis in <i<P. leopardus</i<, contributing to the broader understanding of fish reproductive biology and aquaculture breeding.
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title_short	Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<)
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Theoretical Analysis and Expression Profiling of 17β-Hydroxysteroid Dehydrogenase Genes in Gonadal Development and Steroidogenesis of Leopard Coral Grouper (<i<Plectropomus leopardus</i<)

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