<i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice

Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Guang Chen [verfasserIn] Wenli Lian [verfasserIn] Anjing Geng [verfasserIn] Yihan Wang [verfasserIn] Minghao Liu [verfasserIn] Yue Zhang [verfasserIn] Xu Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	rice drought tolerance sugar transport inducible promoter

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 25(2024), 4, p 2158
Übergeordnetes Werk:	volume:25 ; year:2024 ; number:4, p 2158

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms25042158

Katalog-ID:	DOAJ099633981

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520			\|a Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage.
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700	0		\|a Xu Wang \|e verfasserin \|4 aut
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allfields	10.3390/ijms25042158 doi (DE-627)DOAJ099633981 (DE-599)DOAJf9ea9fa27aee414083e187b20f656e58 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Guang Chen verfasserin aut <i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage. rice drought tolerance sugar transport inducible promoter Biology (General) Chemistry Wenli Lian verfasserin aut Anjing Geng verfasserin aut Yihan Wang verfasserin aut Minghao Liu verfasserin aut Yue Zhang verfasserin aut Xu Wang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2158 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2158 https://doi.org/10.3390/ijms25042158 kostenfrei https://doaj.org/article/f9ea9fa27aee414083e187b20f656e58 kostenfrei https://www.mdpi.com/1422-0067/25/4/2158 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2158
spelling	10.3390/ijms25042158 doi (DE-627)DOAJ099633981 (DE-599)DOAJf9ea9fa27aee414083e187b20f656e58 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Guang Chen verfasserin aut <i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage. rice drought tolerance sugar transport inducible promoter Biology (General) Chemistry Wenli Lian verfasserin aut Anjing Geng verfasserin aut Yihan Wang verfasserin aut Minghao Liu verfasserin aut Yue Zhang verfasserin aut Xu Wang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2158 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2158 https://doi.org/10.3390/ijms25042158 kostenfrei https://doaj.org/article/f9ea9fa27aee414083e187b20f656e58 kostenfrei https://www.mdpi.com/1422-0067/25/4/2158 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2158
allfields_unstemmed	10.3390/ijms25042158 doi (DE-627)DOAJ099633981 (DE-599)DOAJf9ea9fa27aee414083e187b20f656e58 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Guang Chen verfasserin aut <i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage. rice drought tolerance sugar transport inducible promoter Biology (General) Chemistry Wenli Lian verfasserin aut Anjing Geng verfasserin aut Yihan Wang verfasserin aut Minghao Liu verfasserin aut Yue Zhang verfasserin aut Xu Wang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2158 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2158 https://doi.org/10.3390/ijms25042158 kostenfrei https://doaj.org/article/f9ea9fa27aee414083e187b20f656e58 kostenfrei https://www.mdpi.com/1422-0067/25/4/2158 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2158
allfieldsGer	10.3390/ijms25042158 doi (DE-627)DOAJ099633981 (DE-599)DOAJf9ea9fa27aee414083e187b20f656e58 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Guang Chen verfasserin aut <i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage. rice drought tolerance sugar transport inducible promoter Biology (General) Chemistry Wenli Lian verfasserin aut Anjing Geng verfasserin aut Yihan Wang verfasserin aut Minghao Liu verfasserin aut Yue Zhang verfasserin aut Xu Wang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2158 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2158 https://doi.org/10.3390/ijms25042158 kostenfrei https://doaj.org/article/f9ea9fa27aee414083e187b20f656e58 kostenfrei https://www.mdpi.com/1422-0067/25/4/2158 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2158
allfieldsSound	10.3390/ijms25042158 doi (DE-627)DOAJ099633981 (DE-599)DOAJf9ea9fa27aee414083e187b20f656e58 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Guang Chen verfasserin aut <i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage. rice drought tolerance sugar transport inducible promoter Biology (General) Chemistry Wenli Lian verfasserin aut Anjing Geng verfasserin aut Yihan Wang verfasserin aut Minghao Liu verfasserin aut Yue Zhang verfasserin aut Xu Wang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 25(2024), 4, p 2158 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:25 year:2024 number:4, p 2158 https://doi.org/10.3390/ijms25042158 kostenfrei https://doaj.org/article/f9ea9fa27aee414083e187b20f656e58 kostenfrei https://www.mdpi.com/1422-0067/25/4/2158 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2024 4, p 2158
language	English
source	In International Journal of Molecular Sciences 25(2024), 4, p 2158 volume:25 year:2024 number:4, p 2158
sourceStr	In International Journal of Molecular Sciences 25(2024), 4, p 2158 volume:25 year:2024 number:4, p 2158
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	rice drought tolerance sugar transport inducible promoter Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Guang Chen @@aut@@ Wenli Lian @@aut@@ Anjing Geng @@aut@@ Yihan Wang @@aut@@ Minghao Liu @@aut@@ Yue Zhang @@aut@@ Xu Wang @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ099633981
language_de	englisch
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callnumber-first	Q - Science
author	Guang Chen
spellingShingle	Guang Chen misc QH301-705.5 misc QD1-999 misc rice misc drought tolerance misc sugar transport misc inducible promoter misc Biology (General) misc Chemistry <i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice
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topic_title	QH301-705.5 QD1-999 <i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice rice drought tolerance sugar transport inducible promoter
topic	misc QH301-705.5 misc QD1-999 misc rice misc drought tolerance misc sugar transport misc inducible promoter misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc rice misc drought tolerance misc sugar transport misc inducible promoter misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc rice misc drought tolerance misc sugar transport misc inducible promoter misc Biology (General) misc Chemistry
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title	<i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice
ctrlnum	(DE-627)DOAJ099633981 (DE-599)DOAJf9ea9fa27aee414083e187b20f656e58
title_full	<i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice
author_sort	Guang Chen
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
callnumber-first-code	Q
lang_code	eng
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author_browse	Guang Chen Wenli Lian Anjing Geng Yihan Wang Minghao Liu Yue Zhang Xu Wang
container_volume	25
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format_se	Elektronische Aufsätze
author-letter	Guang Chen
doi_str_mv	10.3390/ijms25042158
author2-role	verfasserin
title_sort	<i<poshak1:ossut1</i< promotes sugar transport and enhances drought tolerance in rice
callnumber	QH301-705.5
title_auth	<i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice
abstract	Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage.
abstractGer	Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage.
abstract_unstemmed	Plant cells accumulate osmotic substances (e.g., sugar) to protect cell components and maintain osmotic balance under drought stress conditions. Previous studies found that <i<pOsHAK1:OsFLN2</i< promotes sugar metabolism and improves the drought tolerance of rice plants under drought stress. This study further evaluated the effect of the ectopic expression of the <i<OsSUT1</i< gene driven by the <i<OsHAK1</i< promoter on the sugar transport and drought tolerance of rice. The results showed that the net photosynthetic rate and sucrose phosphate synthase activity of plants expressing the <i<OsSUT1</i< gene were not significantly different from those of wild-type (WT) rice plants under drought conditions. However, the sucrose transport rate in the phloem increased in the transgenic plants, and the sucrose contents were significantly lower in the leaves but significantly higher in the roots of transgenic plants than those in WT plants. The <i<pOsHAK1:OsSUT1</i< and <i<pOsHAK1:OsFLN2</i< transgenic lines had similar rates of long-distance sucrose transport and drought tolerance, which were higher than those of the WT plants. The relative water content of the transgenic plants was higher, while their water loss rate, hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<), and malondialdehyde (MDA) contents were lower than those of the WT plants. The stress-responsive gene <i<OsbZIP23</i< and the antioxidant-related gene <i<OsCATB</i< were significantly upregulated in the drought-treated transgenic lines, while the senescence indicator gene <i<SGR</i< and the stress-responsive gene <i<OsNAC2</i< were down-regulated compared to WT plants. These results showed that promoting the long-distance sugar transport through the expression of <i<pOsHAK1:OsSUT1</i< could produce an improved drought tolerance effect similar to that of <i<pOsHAK1:OsFLN2,</i< providing an effective way to improve the drought tolerance of cereal crops at the seedling stage.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	4, p 2158
title_short	<i<pOsHAK1:OsSUT1</i< Promotes Sugar Transport and Enhances Drought Tolerance in Rice
url	https://doi.org/10.3390/ijms25042158 https://doaj.org/article/f9ea9fa27aee414083e187b20f656e58 https://www.mdpi.com/1422-0067/25/4/2158 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Wenli Lian Anjing Geng Yihan Wang Minghao Liu Yue Zhang Xu Wang
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