Construction of Riboswitches for Screening Antibacterial Agents from Forest Plants
Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from...
Ausführliche Beschreibung
Autor*in: |
Zhanjun Liu [verfasserIn] Taotao Li [verfasserIn] Xingyu Zhang [verfasserIn] Shiquan Liu [verfasserIn] Zhiyuan Hu [verfasserIn] Songlin Yu [verfasserIn] Xiaohong Zhou [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2024 |
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Übergeordnetes Werk: |
In: Forests - MDPI AG, 2010, 15(2024), 2, p 367 |
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Übergeordnetes Werk: |
volume:15 ; year:2024 ; number:2, p 367 |
Links: |
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DOI / URN: |
10.3390/f15020367 |
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Katalog-ID: |
DOAJ099642670 |
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10.3390/f15020367 doi (DE-627)DOAJ099642670 (DE-599)DOAJd434503df0e646d2a7000245557997af DE-627 ger DE-627 rakwb eng QK900-989 Zhanjun Liu verfasserin aut Construction of Riboswitches for Screening Antibacterial Agents from Forest Plants 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. plant bioactive components antibacterial target non-coding RNA antibiotic resistance functional nucleic acid material Plant ecology Taotao Li verfasserin aut Xingyu Zhang verfasserin aut Shiquan Liu verfasserin aut Zhiyuan Hu verfasserin aut Songlin Yu verfasserin aut Xiaohong Zhou verfasserin aut In Forests MDPI AG, 2010 15(2024), 2, p 367 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:15 year:2024 number:2, p 367 https://doi.org/10.3390/f15020367 kostenfrei https://doaj.org/article/d434503df0e646d2a7000245557997af kostenfrei https://www.mdpi.com/1999-4907/15/2/367 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 2, p 367 |
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10.3390/f15020367 doi (DE-627)DOAJ099642670 (DE-599)DOAJd434503df0e646d2a7000245557997af DE-627 ger DE-627 rakwb eng QK900-989 Zhanjun Liu verfasserin aut Construction of Riboswitches for Screening Antibacterial Agents from Forest Plants 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. plant bioactive components antibacterial target non-coding RNA antibiotic resistance functional nucleic acid material Plant ecology Taotao Li verfasserin aut Xingyu Zhang verfasserin aut Shiquan Liu verfasserin aut Zhiyuan Hu verfasserin aut Songlin Yu verfasserin aut Xiaohong Zhou verfasserin aut In Forests MDPI AG, 2010 15(2024), 2, p 367 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:15 year:2024 number:2, p 367 https://doi.org/10.3390/f15020367 kostenfrei https://doaj.org/article/d434503df0e646d2a7000245557997af kostenfrei https://www.mdpi.com/1999-4907/15/2/367 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 2, p 367 |
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10.3390/f15020367 doi (DE-627)DOAJ099642670 (DE-599)DOAJd434503df0e646d2a7000245557997af DE-627 ger DE-627 rakwb eng QK900-989 Zhanjun Liu verfasserin aut Construction of Riboswitches for Screening Antibacterial Agents from Forest Plants 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. plant bioactive components antibacterial target non-coding RNA antibiotic resistance functional nucleic acid material Plant ecology Taotao Li verfasserin aut Xingyu Zhang verfasserin aut Shiquan Liu verfasserin aut Zhiyuan Hu verfasserin aut Songlin Yu verfasserin aut Xiaohong Zhou verfasserin aut In Forests MDPI AG, 2010 15(2024), 2, p 367 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:15 year:2024 number:2, p 367 https://doi.org/10.3390/f15020367 kostenfrei https://doaj.org/article/d434503df0e646d2a7000245557997af kostenfrei https://www.mdpi.com/1999-4907/15/2/367 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 2, p 367 |
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10.3390/f15020367 doi (DE-627)DOAJ099642670 (DE-599)DOAJd434503df0e646d2a7000245557997af DE-627 ger DE-627 rakwb eng QK900-989 Zhanjun Liu verfasserin aut Construction of Riboswitches for Screening Antibacterial Agents from Forest Plants 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. plant bioactive components antibacterial target non-coding RNA antibiotic resistance functional nucleic acid material Plant ecology Taotao Li verfasserin aut Xingyu Zhang verfasserin aut Shiquan Liu verfasserin aut Zhiyuan Hu verfasserin aut Songlin Yu verfasserin aut Xiaohong Zhou verfasserin aut In Forests MDPI AG, 2010 15(2024), 2, p 367 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:15 year:2024 number:2, p 367 https://doi.org/10.3390/f15020367 kostenfrei https://doaj.org/article/d434503df0e646d2a7000245557997af kostenfrei https://www.mdpi.com/1999-4907/15/2/367 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 2, p 367 |
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10.3390/f15020367 doi (DE-627)DOAJ099642670 (DE-599)DOAJd434503df0e646d2a7000245557997af DE-627 ger DE-627 rakwb eng QK900-989 Zhanjun Liu verfasserin aut Construction of Riboswitches for Screening Antibacterial Agents from Forest Plants 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. plant bioactive components antibacterial target non-coding RNA antibiotic resistance functional nucleic acid material Plant ecology Taotao Li verfasserin aut Xingyu Zhang verfasserin aut Shiquan Liu verfasserin aut Zhiyuan Hu verfasserin aut Songlin Yu verfasserin aut Xiaohong Zhou verfasserin aut In Forests MDPI AG, 2010 15(2024), 2, p 367 (DE-627)614095689 (DE-600)2527081-3 19994907 nnns volume:15 year:2024 number:2, p 367 https://doi.org/10.3390/f15020367 kostenfrei https://doaj.org/article/d434503df0e646d2a7000245557997af kostenfrei https://www.mdpi.com/1999-4907/15/2/367 kostenfrei https://doaj.org/toc/1999-4907 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 15 2024 2, p 367 |
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Construction of Riboswitches for Screening Antibacterial Agents from Forest Plants |
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Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. |
abstractGer |
Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. |
abstract_unstemmed |
Forest plants contain abundant natural products, providing a valuable resource for obtaining compounds with various functional activities, such as antimicrobial, lipid-lowering, and immunoregulatory activities. The development of efficient tools for rapidly screening functional natural products from forest plants is essential for human health. In this study, we constructed some transgenic strains (<i<Escherichia coli</i<) containing Ahy1-1 riboswitches that respond to cyclic di-guanylate (c-di-GMP), serving as a novel bacteriostatic target. The Ahy1-1 riboswitches contained the <i<LacZ</i< gene (encoding β-galactosidase) and c-di-GMP aptamer in order to monitor β-galactosidase activity due to changes in c-di-GMP. After co-incubating with extracts from fresh orange peel, fresh tea leaves, and Fuzhuan brick tea, the orange peel exhibited a significant inhibition of c-di-GMP generation. The extract of tea leaves had a minor influence on the synthesis of c-di-GMP, whereas Fuzhuan brick tea, which is fermented by various microorganisms, inhibited the production of c-di-GMP. Our constructed transgenic strains could be used to screen for antibacterial agents from forest plants. Beyond antibacterial agents, other functional compounds from forest plants could be selected by designing diverse riboswitches. |
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