Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis

Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jiangbo An [verfasserIn] Lin Sun [verfasserIn] Mingjian Liu [verfasserIn] Rui Dai [verfasserIn] Qiang Si [verfasserIn] Gentu Ge [verfasserIn] Zhijun Wang [verfasserIn] Yushan Jia [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	physiological characteristics metabolic products nitrites

Übergeordnetes Werk:	In: Fermentation - MDPI AG, 2017, 10(2024), 2, p 92
Übergeordnetes Werk:	volume:10 ; year:2024 ; number:2, p 92

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/fermentation10020092

Katalog-ID:	DOAJ099646625

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allfields	10.3390/fermentation10020092 doi (DE-627)DOAJ099646625 (DE-599)DOAJ689ded3628ed4f44a892ec6483f999c5 DE-627 ger DE-627 rakwb eng TP500-660 Jiangbo An verfasserin aut Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50. <i<Lactiplantibacillus plantarum</i< physiological characteristics metabolic products nitrites Fermentation industries. Beverages. Alcohol Lin Sun verfasserin aut Mingjian Liu verfasserin aut Rui Dai verfasserin aut Qiang Si verfasserin aut Gentu Ge verfasserin aut Zhijun Wang verfasserin aut Yushan Jia verfasserin aut In Fermentation MDPI AG, 2017 10(2024), 2, p 92 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:10 year:2024 number:2, p 92 https://doi.org/10.3390/fermentation10020092 kostenfrei https://doaj.org/article/689ded3628ed4f44a892ec6483f999c5 kostenfrei https://www.mdpi.com/2311-5637/10/2/92 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2024 2, p 92
spelling	10.3390/fermentation10020092 doi (DE-627)DOAJ099646625 (DE-599)DOAJ689ded3628ed4f44a892ec6483f999c5 DE-627 ger DE-627 rakwb eng TP500-660 Jiangbo An verfasserin aut Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50. <i<Lactiplantibacillus plantarum</i< physiological characteristics metabolic products nitrites Fermentation industries. Beverages. Alcohol Lin Sun verfasserin aut Mingjian Liu verfasserin aut Rui Dai verfasserin aut Qiang Si verfasserin aut Gentu Ge verfasserin aut Zhijun Wang verfasserin aut Yushan Jia verfasserin aut In Fermentation MDPI AG, 2017 10(2024), 2, p 92 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:10 year:2024 number:2, p 92 https://doi.org/10.3390/fermentation10020092 kostenfrei https://doaj.org/article/689ded3628ed4f44a892ec6483f999c5 kostenfrei https://www.mdpi.com/2311-5637/10/2/92 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2024 2, p 92
allfields_unstemmed	10.3390/fermentation10020092 doi (DE-627)DOAJ099646625 (DE-599)DOAJ689ded3628ed4f44a892ec6483f999c5 DE-627 ger DE-627 rakwb eng TP500-660 Jiangbo An verfasserin aut Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50. <i<Lactiplantibacillus plantarum</i< physiological characteristics metabolic products nitrites Fermentation industries. Beverages. Alcohol Lin Sun verfasserin aut Mingjian Liu verfasserin aut Rui Dai verfasserin aut Qiang Si verfasserin aut Gentu Ge verfasserin aut Zhijun Wang verfasserin aut Yushan Jia verfasserin aut In Fermentation MDPI AG, 2017 10(2024), 2, p 92 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:10 year:2024 number:2, p 92 https://doi.org/10.3390/fermentation10020092 kostenfrei https://doaj.org/article/689ded3628ed4f44a892ec6483f999c5 kostenfrei https://www.mdpi.com/2311-5637/10/2/92 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2024 2, p 92
allfieldsGer	10.3390/fermentation10020092 doi (DE-627)DOAJ099646625 (DE-599)DOAJ689ded3628ed4f44a892ec6483f999c5 DE-627 ger DE-627 rakwb eng TP500-660 Jiangbo An verfasserin aut Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50. <i<Lactiplantibacillus plantarum</i< physiological characteristics metabolic products nitrites Fermentation industries. Beverages. Alcohol Lin Sun verfasserin aut Mingjian Liu verfasserin aut Rui Dai verfasserin aut Qiang Si verfasserin aut Gentu Ge verfasserin aut Zhijun Wang verfasserin aut Yushan Jia verfasserin aut In Fermentation MDPI AG, 2017 10(2024), 2, p 92 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:10 year:2024 number:2, p 92 https://doi.org/10.3390/fermentation10020092 kostenfrei https://doaj.org/article/689ded3628ed4f44a892ec6483f999c5 kostenfrei https://www.mdpi.com/2311-5637/10/2/92 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2024 2, p 92
allfieldsSound	10.3390/fermentation10020092 doi (DE-627)DOAJ099646625 (DE-599)DOAJ689ded3628ed4f44a892ec6483f999c5 DE-627 ger DE-627 rakwb eng TP500-660 Jiangbo An verfasserin aut Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50. <i<Lactiplantibacillus plantarum</i< physiological characteristics metabolic products nitrites Fermentation industries. Beverages. Alcohol Lin Sun verfasserin aut Mingjian Liu verfasserin aut Rui Dai verfasserin aut Qiang Si verfasserin aut Gentu Ge verfasserin aut Zhijun Wang verfasserin aut Yushan Jia verfasserin aut In Fermentation MDPI AG, 2017 10(2024), 2, p 92 (DE-627)820684163 (DE-600)2813985-9 23115637 nnns volume:10 year:2024 number:2, p 92 https://doi.org/10.3390/fermentation10020092 kostenfrei https://doaj.org/article/689ded3628ed4f44a892ec6483f999c5 kostenfrei https://www.mdpi.com/2311-5637/10/2/92 kostenfrei https://doaj.org/toc/2311-5637 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2024 2, p 92
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spellingShingle	Jiangbo An misc TP500-660 misc <i<Lactiplantibacillus plantarum</i< misc physiological characteristics misc metabolic products misc nitrites misc Fermentation industries. Beverages. Alcohol Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis
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topic_title	TP500-660 Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis <i<Lactiplantibacillus plantarum</i< physiological characteristics metabolic products nitrites
topic	misc TP500-660 misc <i<Lactiplantibacillus plantarum</i< misc physiological characteristics misc metabolic products misc nitrites misc Fermentation industries. Beverages. Alcohol
topic_unstemmed	misc TP500-660 misc <i<Lactiplantibacillus plantarum</i< misc physiological characteristics misc metabolic products misc nitrites misc Fermentation industries. Beverages. Alcohol
topic_browse	misc TP500-660 misc <i<Lactiplantibacillus plantarum</i< misc physiological characteristics misc metabolic products misc nitrites misc Fermentation industries. Beverages. Alcohol
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title	Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis
ctrlnum	(DE-627)DOAJ099646625 (DE-599)DOAJ689ded3628ed4f44a892ec6483f999c5
title_full	Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis
author_sort	Jiangbo An
journal	Fermentation
journalStr	Fermentation
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author_browse	Jiangbo An Lin Sun Mingjian Liu Rui Dai Qiang Si Gentu Ge Zhijun Wang Yushan Jia
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author-letter	Jiangbo An
doi_str_mv	10.3390/fermentation10020092
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title_sort	mechanistic insights into nitrite degradation by metabolites of <i<l. plantarum</i< a50: an lc-ms-based untargeted metabolomics analysis
callnumber	TP500-660
title_auth	Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis
abstract	Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50.
abstractGer	Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50.
abstract_unstemmed	Nitrites are universally acknowledged natural toxic substances that frequently lead to poisoning in humans and animals. During fermentation, certain microorganisms utilize a portion of the nitrogen element and reduce nitrates to nitrites through specific metabolic pathways. In this study, a highly effective lactic acid bacterial strain, <i<Lactiplantibacillus plantarum</i< A50, was isolated and screened from alfalfa silage for its remarkable ability to degrade nitrites. <i<L. plantarum</i< A50 exhibits exceptional nitrite removal capacity, with a degradation rate of 99.06% within 24 h. Furthermore, <i<L. plantarum</i< A50 demonstrates normal growth under pH values ranging from 4 to 9 and salt concentrations of 5%, displaying excellent tolerance to acidity, alkalinity, and salinity. Additionally, it undergoes fermentation using various carbon sources. Within the first 6–12 h of culture, <i<L. plantarum</i< A50 primarily achieves nitrite degradation through non-acidic processes, resulting in a degradation rate of 82.67% by the 12th hour. Moreover, the metabolites produced by <i<L. plantarum</i< A50 exhibit a synergistic interaction with acidity, leading to a nitrite degradation rate of 98.48% within 24 h. Notably, both <i<L. plantarum</i< A50 and MRS broth were found to degrade nitrites. Consequently, a non-targeted metabolomic analysis using LC-MS was conducted to identify 342 significantly different metabolites between <i<L. plantarum</i< A50 and MRS broth. Among these, lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, and organoheterocyclic compounds emerged as the main constituents. Lipids and lipid-like molecules, derivatives of glucose and galactose, amino acids and their derivatives, as well as organoheterocyclic compounds, are likely to play a role in nitrite elimination. Through the enrichment analysis of differential metabolic pathways using KEGG, nine distinct pathways were identified. These pathways provide essential nutrients, maintain cellular structure and function, participate in substance transport, regulate metabolic activities, and enhance resistance against pathogenic microorganisms in <i<L. plantarum</i< A50.
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container_issue	2, p 92
title_short	Mechanistic Insights into Nitrite Degradation by Metabolites of <i<L. plantarum</i< A50: An LC-MS-Based Untargeted Metabolomics Analysis
url	https://doi.org/10.3390/fermentation10020092 https://doaj.org/article/689ded3628ed4f44a892ec6483f999c5 https://www.mdpi.com/2311-5637/10/2/92 https://doaj.org/toc/2311-5637
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author2	Lin Sun Mingjian Liu Rui Dai Qiang Si Gentu Ge Zhijun Wang Yushan Jia
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up_date	2024-07-03T23:45:38.002Z
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