ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer

Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specifici...
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Autor*in:	Serena M. Vilasi [verfasserIn] Jannett Nguyen [verfasserIn] Catherine J. Wang [verfasserIn] Lingling Miao [verfasserIn] Kenneth Daily [verfasserIn] Mary Eid [verfasserIn] Joon Seon Song [verfasserIn] Hong Jiang [verfasserIn] Kris Ylaya [verfasserIn] Klaus J. Busam [verfasserIn] Maria R. Gaiser [verfasserIn] Stephen M. Hewitt [verfasserIn] Isaac Brownell [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6

Übergeordnetes Werk:	In: Cancers - MDPI AG, 2010, 16(2024), 4, p 788
Übergeordnetes Werk:	volume:16 ; year:2024 ; number:4, p 788

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cancers16040788

Katalog-ID:	DOAJ099662302

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allfields	10.3390/cancers16040788 doi (DE-627)DOAJ099662302 (DE-599)DOAJ41c2cd9c8ce748ab8d24b45c7745c8fc DE-627 ger DE-627 rakwb eng RC254-282 Serena M. Vilasi verfasserin aut ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC. Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Jannett Nguyen verfasserin aut Catherine J. Wang verfasserin aut Lingling Miao verfasserin aut Kenneth Daily verfasserin aut Mary Eid verfasserin aut Joon Seon Song verfasserin aut Hong Jiang verfasserin aut Kris Ylaya verfasserin aut Klaus J. Busam verfasserin aut Maria R. Gaiser verfasserin aut Stephen M. Hewitt verfasserin aut Isaac Brownell verfasserin aut In Cancers MDPI AG, 2010 16(2024), 4, p 788 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:16 year:2024 number:4, p 788 https://doi.org/10.3390/cancers16040788 kostenfrei https://doaj.org/article/41c2cd9c8ce748ab8d24b45c7745c8fc kostenfrei https://www.mdpi.com/2072-6694/16/4/788 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2024 4, p 788
spelling	10.3390/cancers16040788 doi (DE-627)DOAJ099662302 (DE-599)DOAJ41c2cd9c8ce748ab8d24b45c7745c8fc DE-627 ger DE-627 rakwb eng RC254-282 Serena M. Vilasi verfasserin aut ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC. Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Jannett Nguyen verfasserin aut Catherine J. Wang verfasserin aut Lingling Miao verfasserin aut Kenneth Daily verfasserin aut Mary Eid verfasserin aut Joon Seon Song verfasserin aut Hong Jiang verfasserin aut Kris Ylaya verfasserin aut Klaus J. Busam verfasserin aut Maria R. Gaiser verfasserin aut Stephen M. Hewitt verfasserin aut Isaac Brownell verfasserin aut In Cancers MDPI AG, 2010 16(2024), 4, p 788 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:16 year:2024 number:4, p 788 https://doi.org/10.3390/cancers16040788 kostenfrei https://doaj.org/article/41c2cd9c8ce748ab8d24b45c7745c8fc kostenfrei https://www.mdpi.com/2072-6694/16/4/788 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2024 4, p 788
allfields_unstemmed	10.3390/cancers16040788 doi (DE-627)DOAJ099662302 (DE-599)DOAJ41c2cd9c8ce748ab8d24b45c7745c8fc DE-627 ger DE-627 rakwb eng RC254-282 Serena M. Vilasi verfasserin aut ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC. Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Jannett Nguyen verfasserin aut Catherine J. Wang verfasserin aut Lingling Miao verfasserin aut Kenneth Daily verfasserin aut Mary Eid verfasserin aut Joon Seon Song verfasserin aut Hong Jiang verfasserin aut Kris Ylaya verfasserin aut Klaus J. Busam verfasserin aut Maria R. Gaiser verfasserin aut Stephen M. Hewitt verfasserin aut Isaac Brownell verfasserin aut In Cancers MDPI AG, 2010 16(2024), 4, p 788 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:16 year:2024 number:4, p 788 https://doi.org/10.3390/cancers16040788 kostenfrei https://doaj.org/article/41c2cd9c8ce748ab8d24b45c7745c8fc kostenfrei https://www.mdpi.com/2072-6694/16/4/788 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2024 4, p 788
allfieldsGer	10.3390/cancers16040788 doi (DE-627)DOAJ099662302 (DE-599)DOAJ41c2cd9c8ce748ab8d24b45c7745c8fc DE-627 ger DE-627 rakwb eng RC254-282 Serena M. Vilasi verfasserin aut ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC. Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Jannett Nguyen verfasserin aut Catherine J. Wang verfasserin aut Lingling Miao verfasserin aut Kenneth Daily verfasserin aut Mary Eid verfasserin aut Joon Seon Song verfasserin aut Hong Jiang verfasserin aut Kris Ylaya verfasserin aut Klaus J. Busam verfasserin aut Maria R. Gaiser verfasserin aut Stephen M. Hewitt verfasserin aut Isaac Brownell verfasserin aut In Cancers MDPI AG, 2010 16(2024), 4, p 788 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:16 year:2024 number:4, p 788 https://doi.org/10.3390/cancers16040788 kostenfrei https://doaj.org/article/41c2cd9c8ce748ab8d24b45c7745c8fc kostenfrei https://www.mdpi.com/2072-6694/16/4/788 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2024 4, p 788
allfieldsSound	10.3390/cancers16040788 doi (DE-627)DOAJ099662302 (DE-599)DOAJ41c2cd9c8ce748ab8d24b45c7745c8fc DE-627 ger DE-627 rakwb eng RC254-282 Serena M. Vilasi verfasserin aut ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC. Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Jannett Nguyen verfasserin aut Catherine J. Wang verfasserin aut Lingling Miao verfasserin aut Kenneth Daily verfasserin aut Mary Eid verfasserin aut Joon Seon Song verfasserin aut Hong Jiang verfasserin aut Kris Ylaya verfasserin aut Klaus J. Busam verfasserin aut Maria R. Gaiser verfasserin aut Stephen M. Hewitt verfasserin aut Isaac Brownell verfasserin aut In Cancers MDPI AG, 2010 16(2024), 4, p 788 (DE-627)614095670 (DE-600)2527080-1 20726694 nnns volume:16 year:2024 number:4, p 788 https://doi.org/10.3390/cancers16040788 kostenfrei https://doaj.org/article/41c2cd9c8ce748ab8d24b45c7745c8fc kostenfrei https://www.mdpi.com/2072-6694/16/4/788 kostenfrei https://doaj.org/toc/2072-6694 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2024 4, p 788
language	English
source	In Cancers 16(2024), 4, p 788 volume:16 year:2024 number:4, p 788
sourceStr	In Cancers 16(2024), 4, p 788 volume:16 year:2024 number:4, p 788
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6 Neoplasms. Tumors. Oncology. Including cancer and carcinogens
isfreeaccess_bool	true
container_title	Cancers
authorswithroles_txt_mv	Serena M. Vilasi @@aut@@ Jannett Nguyen @@aut@@ Catherine J. Wang @@aut@@ Lingling Miao @@aut@@ Kenneth Daily @@aut@@ Mary Eid @@aut@@ Joon Seon Song @@aut@@ Hong Jiang @@aut@@ Kris Ylaya @@aut@@ Klaus J. Busam @@aut@@ Maria R. Gaiser @@aut@@ Stephen M. Hewitt @@aut@@ Isaac Brownell @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	614095670
id	DOAJ099662302
language_de	englisch
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callnumber-first	R - Medicine
author	Serena M. Vilasi
spellingShingle	Serena M. Vilasi misc RC254-282 misc Merkel cell carcinoma misc small cell lung cancer misc neuroendocrine tumor misc ATOH1 misc TFAP2B misc CEACAM6 misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer
authorStr	Serena M. Vilasi
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topic_title	RC254-282 ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer Merkel cell carcinoma small cell lung cancer neuroendocrine tumor ATOH1 TFAP2B CEACAM6
topic	misc RC254-282 misc Merkel cell carcinoma misc small cell lung cancer misc neuroendocrine tumor misc ATOH1 misc TFAP2B misc CEACAM6 misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc Merkel cell carcinoma misc small cell lung cancer misc neuroendocrine tumor misc ATOH1 misc TFAP2B misc CEACAM6 misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_browse	misc RC254-282 misc Merkel cell carcinoma misc small cell lung cancer misc neuroendocrine tumor misc ATOH1 misc TFAP2B misc CEACAM6 misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer
ctrlnum	(DE-627)DOAJ099662302 (DE-599)DOAJ41c2cd9c8ce748ab8d24b45c7745c8fc
title_full	ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer
author_sort	Serena M. Vilasi
journal	Cancers
journalStr	Cancers
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author_browse	Serena M. Vilasi Jannett Nguyen Catherine J. Wang Lingling Miao Kenneth Daily Mary Eid Joon Seon Song Hong Jiang Kris Ylaya Klaus J. Busam Maria R. Gaiser Stephen M. Hewitt Isaac Brownell
container_volume	16
class	RC254-282
format_se	Elektronische Aufsätze
author-letter	Serena M. Vilasi
doi_str_mv	10.3390/cancers16040788
author2-role	verfasserin
title_sort	atoh1, tfap2b, and ceacam6 as immunohistochemical markers to distinguish merkel cell carcinoma and small cell lung cancer
callnumber	RC254-282
title_auth	ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer
abstract	Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC.
abstractGer	Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC.
abstract_unstemmed	Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC.
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container_issue	4, p 788
title_short	ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer
url	https://doi.org/10.3390/cancers16040788 https://doaj.org/article/41c2cd9c8ce748ab8d24b45c7745c8fc https://www.mdpi.com/2072-6694/16/4/788 https://doaj.org/toc/2072-6694
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author2	Jannett Nguyen Catherine J. Wang Lingling Miao Kenneth Daily Mary Eid Joon Seon Song Hong Jiang Kris Ylaya Klaus J. Busam Maria R. Gaiser Stephen M. Hewitt Isaac Brownell
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up_date	2024-07-03T23:49:54.661Z
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ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer

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