Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices

Amperometry is arguably the most widely used technique for studying the exocytosis of biological amines. However, the scarcity of human tissues, particularly in the context of neurological diseases, poses a challenge for exocytosis research. Human platelets, which accumulate 90% of blood serotonin,...
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Autor*in:	Rosalía González Brito [verfasserIn] Pablo Montenegro [verfasserIn] Alicia Méndez [verfasserIn] Ramtin E. Shabgahi [verfasserIn] Alberto Pasquarelli [verfasserIn] Ricardo Borges [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	amperometry electrochemistry exocytosis serotonin human platelets

Übergeordnetes Werk:	In: Biosensors - MDPI AG, 2012, 14(2024), 2, p 75
Übergeordnetes Werk:	volume:14 ; year:2024 ; number:2, p 75

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/bios14020075

Katalog-ID:	DOAJ099667096

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allfieldsSound	10.3390/bios14020075 doi (DE-627)DOAJ099667096 (DE-599)DOAJ482402ade08f4fc38b75bbc25bc8b132 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Rosalía González Brito verfasserin aut Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Amperometry is arguably the most widely used technique for studying the exocytosis of biological amines. However, the scarcity of human tissues, particularly in the context of neurological diseases, poses a challenge for exocytosis research. Human platelets, which accumulate 90% of blood serotonin, release it through exocytosis. Nevertheless, single-cell amperometry with encapsulated carbon fibers is impractical due to the small size of platelets and the limited number of secretory granules on each platelet. The recent technological improvements in amperometric multi-electrode array (MEA) devices allow simultaneous recordings from several high-performance electrodes. In this paper, we present a comparison of three MEA boron-doped diamond (BDD) devices for studying serotonin exocytosis in human platelets: (i) the BDD-on-glass MEA, (ii) the BDD-on-silicon MEA, and (iii) the BDD on amorphous quartz MEA (BDD-on-quartz MEA). Transparent electrodes offer several advantages for observing living cells, and in the case of platelets, they control activation/aggregation. BDD-on-quartz offers the advantage over previous materials of combining excellent electrochemical properties with transparency for microscopic observation. These devices are opening exciting perspectives for clinical applications. amperometry electrochemistry exocytosis serotonin human platelets Biotechnology Pablo Montenegro verfasserin aut Alicia Méndez verfasserin aut Ramtin E. Shabgahi verfasserin aut Alberto Pasquarelli verfasserin aut Ricardo Borges verfasserin aut In Biosensors MDPI AG, 2012 14(2024), 2, p 75 (DE-627)718626451 (DE-600)2662125-3 20796374 nnns volume:14 year:2024 number:2, p 75 https://doi.org/10.3390/bios14020075 kostenfrei https://doaj.org/article/482402ade08f4fc38b75bbc25bc8b132 kostenfrei https://www.mdpi.com/2079-6374/14/2/75 kostenfrei https://doaj.org/toc/2079-6374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2024 2, p 75
language	English
source	In Biosensors 14(2024), 2, p 75 volume:14 year:2024 number:2, p 75
sourceStr	In Biosensors 14(2024), 2, p 75 volume:14 year:2024 number:2, p 75
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	amperometry electrochemistry exocytosis serotonin human platelets Biotechnology
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container_title	Biosensors
authorswithroles_txt_mv	Rosalía González Brito @@aut@@ Pablo Montenegro @@aut@@ Alicia Méndez @@aut@@ Ramtin E. Shabgahi @@aut@@ Alberto Pasquarelli @@aut@@ Ricardo Borges @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
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callnumber-first	T - Technology
author	Rosalía González Brito
spellingShingle	Rosalía González Brito misc TP248.13-248.65 misc amperometry misc electrochemistry misc exocytosis misc serotonin misc human platelets misc Biotechnology Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices
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topic_title	TP248.13-248.65 Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices amperometry electrochemistry exocytosis serotonin human platelets
topic	misc TP248.13-248.65 misc amperometry misc electrochemistry misc exocytosis misc serotonin misc human platelets misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc amperometry misc electrochemistry misc exocytosis misc serotonin misc human platelets misc Biotechnology
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title	Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices
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title_full	Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices
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author_browse	Rosalía González Brito Pablo Montenegro Alicia Méndez Ramtin E. Shabgahi Alberto Pasquarelli Ricardo Borges
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title_sort	analytical determination of serotonin exocytosis in human platelets with bdd-on-quartz mea devices
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title_auth	Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices
abstract	Amperometry is arguably the most widely used technique for studying the exocytosis of biological amines. However, the scarcity of human tissues, particularly in the context of neurological diseases, poses a challenge for exocytosis research. Human platelets, which accumulate 90% of blood serotonin, release it through exocytosis. Nevertheless, single-cell amperometry with encapsulated carbon fibers is impractical due to the small size of platelets and the limited number of secretory granules on each platelet. The recent technological improvements in amperometric multi-electrode array (MEA) devices allow simultaneous recordings from several high-performance electrodes. In this paper, we present a comparison of three MEA boron-doped diamond (BDD) devices for studying serotonin exocytosis in human platelets: (i) the BDD-on-glass MEA, (ii) the BDD-on-silicon MEA, and (iii) the BDD on amorphous quartz MEA (BDD-on-quartz MEA). Transparent electrodes offer several advantages for observing living cells, and in the case of platelets, they control activation/aggregation. BDD-on-quartz offers the advantage over previous materials of combining excellent electrochemical properties with transparency for microscopic observation. These devices are opening exciting perspectives for clinical applications.
abstractGer	Amperometry is arguably the most widely used technique for studying the exocytosis of biological amines. However, the scarcity of human tissues, particularly in the context of neurological diseases, poses a challenge for exocytosis research. Human platelets, which accumulate 90% of blood serotonin, release it through exocytosis. Nevertheless, single-cell amperometry with encapsulated carbon fibers is impractical due to the small size of platelets and the limited number of secretory granules on each platelet. The recent technological improvements in amperometric multi-electrode array (MEA) devices allow simultaneous recordings from several high-performance electrodes. In this paper, we present a comparison of three MEA boron-doped diamond (BDD) devices for studying serotonin exocytosis in human platelets: (i) the BDD-on-glass MEA, (ii) the BDD-on-silicon MEA, and (iii) the BDD on amorphous quartz MEA (BDD-on-quartz MEA). Transparent electrodes offer several advantages for observing living cells, and in the case of platelets, they control activation/aggregation. BDD-on-quartz offers the advantage over previous materials of combining excellent electrochemical properties with transparency for microscopic observation. These devices are opening exciting perspectives for clinical applications.
abstract_unstemmed	Amperometry is arguably the most widely used technique for studying the exocytosis of biological amines. However, the scarcity of human tissues, particularly in the context of neurological diseases, poses a challenge for exocytosis research. Human platelets, which accumulate 90% of blood serotonin, release it through exocytosis. Nevertheless, single-cell amperometry with encapsulated carbon fibers is impractical due to the small size of platelets and the limited number of secretory granules on each platelet. The recent technological improvements in amperometric multi-electrode array (MEA) devices allow simultaneous recordings from several high-performance electrodes. In this paper, we present a comparison of three MEA boron-doped diamond (BDD) devices for studying serotonin exocytosis in human platelets: (i) the BDD-on-glass MEA, (ii) the BDD-on-silicon MEA, and (iii) the BDD on amorphous quartz MEA (BDD-on-quartz MEA). Transparent electrodes offer several advantages for observing living cells, and in the case of platelets, they control activation/aggregation. BDD-on-quartz offers the advantage over previous materials of combining excellent electrochemical properties with transparency for microscopic observation. These devices are opening exciting perspectives for clinical applications.
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title_short	Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices
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Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices

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