Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies

ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obta...
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Autor*in:	Mateusz Rybka [verfasserIn] Łukasz Mazurek [verfasserIn] Joanna Czuwara [verfasserIn] Anna Laskowska [verfasserIn] Mateusz Szudzik [verfasserIn] Zbigniew Ruszczak [verfasserIn] Dorota Sulejczak [verfasserIn] Robert A. Schwartz [verfasserIn] Ewa Kłodzińska [verfasserIn] Adrian Drapała [verfasserIn] Marek Konop [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Keratin fibers natural-derived dressing wound healing biphalin diabetic mouse model 角蛋白纤维

Übergeordnetes Werk:	In: Journal of Natural Fibers - Taylor & Francis Group, 2023, 21(2024), 1
Übergeordnetes Werk:	volume:21 ; year:2024 ; number:1

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1080/15440478.2023.2287647

Katalog-ID:	DOAJ100122124

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520			\|a ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice.
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spelling	10.1080/15440478.2023.2287647 doi (DE-627)DOAJ100122124 (DE-599)DOAJ61bf810d1c89485a8e106239a4716ed0 DE-627 ger DE-627 rakwb eng TP890-933 Mateusz Rybka verfasserin aut Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice. Keratin fibers natural-derived dressing wound healing biphalin diabetic mouse model 角蛋白纤维 Science Q Textile bleaching, dyeing, printing, etc. Łukasz Mazurek verfasserin aut Joanna Czuwara verfasserin aut Anna Laskowska verfasserin aut Mateusz Szudzik verfasserin aut Zbigniew Ruszczak verfasserin aut Dorota Sulejczak verfasserin aut Robert A. Schwartz verfasserin aut Ewa Kłodzińska verfasserin aut Adrian Drapała verfasserin aut Marek Konop verfasserin aut In Journal of Natural Fibers Taylor & Francis Group, 2023 21(2024), 1 (DE-627)486723828 (DE-600)2187542-X 1544046X nnns volume:21 year:2024 number:1 https://doi.org/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/article/61bf810d1c89485a8e106239a4716ed0 kostenfrei https://www.tandfonline.com/doi/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/toc/1544-0478 Journal toc kostenfrei https://doaj.org/toc/1544-046X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 21 2024 1
allfields_unstemmed	10.1080/15440478.2023.2287647 doi (DE-627)DOAJ100122124 (DE-599)DOAJ61bf810d1c89485a8e106239a4716ed0 DE-627 ger DE-627 rakwb eng TP890-933 Mateusz Rybka verfasserin aut Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice. Keratin fibers natural-derived dressing wound healing biphalin diabetic mouse model 角蛋白纤维 Science Q Textile bleaching, dyeing, printing, etc. Łukasz Mazurek verfasserin aut Joanna Czuwara verfasserin aut Anna Laskowska verfasserin aut Mateusz Szudzik verfasserin aut Zbigniew Ruszczak verfasserin aut Dorota Sulejczak verfasserin aut Robert A. Schwartz verfasserin aut Ewa Kłodzińska verfasserin aut Adrian Drapała verfasserin aut Marek Konop verfasserin aut In Journal of Natural Fibers Taylor & Francis Group, 2023 21(2024), 1 (DE-627)486723828 (DE-600)2187542-X 1544046X nnns volume:21 year:2024 number:1 https://doi.org/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/article/61bf810d1c89485a8e106239a4716ed0 kostenfrei https://www.tandfonline.com/doi/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/toc/1544-0478 Journal toc kostenfrei https://doaj.org/toc/1544-046X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 21 2024 1
allfieldsGer	10.1080/15440478.2023.2287647 doi (DE-627)DOAJ100122124 (DE-599)DOAJ61bf810d1c89485a8e106239a4716ed0 DE-627 ger DE-627 rakwb eng TP890-933 Mateusz Rybka verfasserin aut Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice. Keratin fibers natural-derived dressing wound healing biphalin diabetic mouse model 角蛋白纤维 Science Q Textile bleaching, dyeing, printing, etc. Łukasz Mazurek verfasserin aut Joanna Czuwara verfasserin aut Anna Laskowska verfasserin aut Mateusz Szudzik verfasserin aut Zbigniew Ruszczak verfasserin aut Dorota Sulejczak verfasserin aut Robert A. Schwartz verfasserin aut Ewa Kłodzińska verfasserin aut Adrian Drapała verfasserin aut Marek Konop verfasserin aut In Journal of Natural Fibers Taylor & Francis Group, 2023 21(2024), 1 (DE-627)486723828 (DE-600)2187542-X 1544046X nnns volume:21 year:2024 number:1 https://doi.org/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/article/61bf810d1c89485a8e106239a4716ed0 kostenfrei https://www.tandfonline.com/doi/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/toc/1544-0478 Journal toc kostenfrei https://doaj.org/toc/1544-046X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 21 2024 1
allfieldsSound	10.1080/15440478.2023.2287647 doi (DE-627)DOAJ100122124 (DE-599)DOAJ61bf810d1c89485a8e106239a4716ed0 DE-627 ger DE-627 rakwb eng TP890-933 Mateusz Rybka verfasserin aut Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice. Keratin fibers natural-derived dressing wound healing biphalin diabetic mouse model 角蛋白纤维 Science Q Textile bleaching, dyeing, printing, etc. Łukasz Mazurek verfasserin aut Joanna Czuwara verfasserin aut Anna Laskowska verfasserin aut Mateusz Szudzik verfasserin aut Zbigniew Ruszczak verfasserin aut Dorota Sulejczak verfasserin aut Robert A. Schwartz verfasserin aut Ewa Kłodzińska verfasserin aut Adrian Drapała verfasserin aut Marek Konop verfasserin aut In Journal of Natural Fibers Taylor & Francis Group, 2023 21(2024), 1 (DE-627)486723828 (DE-600)2187542-X 1544046X nnns volume:21 year:2024 number:1 https://doi.org/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/article/61bf810d1c89485a8e106239a4716ed0 kostenfrei https://www.tandfonline.com/doi/10.1080/15440478.2023.2287647 kostenfrei https://doaj.org/toc/1544-0478 Journal toc kostenfrei https://doaj.org/toc/1544-046X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2111 GBV_ILN_2129 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 21 2024 1
language	English
source	In Journal of Natural Fibers 21(2024), 1 volume:21 year:2024 number:1
sourceStr	In Journal of Natural Fibers 21(2024), 1 volume:21 year:2024 number:1
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Keratin fibers natural-derived dressing wound healing biphalin diabetic mouse model 角蛋白纤维 Science Q Textile bleaching, dyeing, printing, etc.
isfreeaccess_bool	true
container_title	Journal of Natural Fibers
authorswithroles_txt_mv	Mateusz Rybka @@aut@@ Łukasz Mazurek @@aut@@ Joanna Czuwara @@aut@@ Anna Laskowska @@aut@@ Mateusz Szudzik @@aut@@ Zbigniew Ruszczak @@aut@@ Dorota Sulejczak @@aut@@ Robert A. Schwartz @@aut@@ Ewa Kłodzińska @@aut@@ Adrian Drapała @@aut@@ Marek Konop @@aut@@
publishDateDaySort_date	2024-01-01T00:00:00Z
hierarchy_top_id	486723828
id	DOAJ100122124
language_de	englisch
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Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. 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callnumber-first	T - Technology
author	Mateusz Rybka
spellingShingle	Mateusz Rybka misc TP890-933 misc Keratin fibers misc natural-derived dressing misc wound healing misc biphalin misc diabetic mouse model misc 角蛋白纤维 misc Science misc Q misc Textile bleaching, dyeing, printing, etc. Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies
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topic_title	TP890-933 Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies Keratin fibers natural-derived dressing wound healing biphalin diabetic mouse model 角蛋白纤维
topic	misc TP890-933 misc Keratin fibers misc natural-derived dressing misc wound healing misc biphalin misc diabetic mouse model misc 角蛋白纤维 misc Science misc Q misc Textile bleaching, dyeing, printing, etc.
topic_unstemmed	misc TP890-933 misc Keratin fibers misc natural-derived dressing misc wound healing misc biphalin misc diabetic mouse model misc 角蛋白纤维 misc Science misc Q misc Textile bleaching, dyeing, printing, etc.
topic_browse	misc TP890-933 misc Keratin fibers misc natural-derived dressing misc wound healing misc biphalin misc diabetic mouse model misc 角蛋白纤维 misc Science misc Q misc Textile bleaching, dyeing, printing, etc.
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title	Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies
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title_full	Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies
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author_browse	Mateusz Rybka Łukasz Mazurek Joanna Czuwara Anna Laskowska Mateusz Szudzik Zbigniew Ruszczak Dorota Sulejczak Robert A. Schwartz Ewa Kłodzińska Adrian Drapała Marek Konop
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doi_str_mv	10.1080/15440478.2023.2287647
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title_sort	biomedical potential of keratin-biphalin wound dressing in diabetic mice: in vitro and in vivo studies
callnumber	TP890-933
title_auth	Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies
abstract	ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice.
abstractGer	ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice.
abstract_unstemmed	ABSTRACTStrategies for successful healing of diabetic wounds are lacking. Natural-derived dressings have gained much attention due to their promising properties. We hypothesized that keratin-biphalin fiber-dressing accelerates skin wound healing in a mouse model of diabetes. Keratin fibers were obtained from mouse fur and soaked in a biphalin solution. Studies on NIH/3T3 cells showed that keratin-biphalin fibers increase cell viability. In vivo studies were made on C57BL/6J mice with iatrogenically induced diabetes. Two full-thickness wounds were created on the back of the mice, one untreated and the other treated with an experimental dressing. Western blot analysis, histopathological and immunohistochemical staining were performed. Biphalin was slowly released from keratin fibers. The obtained keratin-biphalin fibers were biocompatible and supported cell growth. Western blot analysis showed that cells treated with our dressing had an increasing expression level of mTOR, and p-AKT 72 h post-treatment compared to 24 h and 48 h. Animal studies showed that the keratin-biphalin dressing accelerated wound healing on days 5 and 15. Treated wounds showed faster reepithelization and developed thicker epidermis. Histopathological and immunohistochemical studies confirmed that keratin-biphalin fibers stimulate macrophage infiltration, which promotes tissue remodeling and regeneration. Exogenous keratin-biphalin fibers p-AKT/mTOR expression protein in vitro and accelerate skin wound healing in diabetic mice.
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title_short	Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies
url	https://doi.org/10.1080/15440478.2023.2287647 https://doaj.org/article/61bf810d1c89485a8e106239a4716ed0 https://www.tandfonline.com/doi/10.1080/15440478.2023.2287647 https://doaj.org/toc/1544-0478 https://doaj.org/toc/1544-046X
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author2	Łukasz Mazurek Joanna Czuwara Anna Laskowska Mateusz Szudzik Zbigniew Ruszczak Dorota Sulejczak Robert A. Schwartz Ewa Kłodzińska Adrian Drapała Marek Konop
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Biomedical Potential of Keratin-Biphalin Wound Dressing in Diabetic Mice: In Vitro and In Vivo Studies

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