Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1

Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nut...
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Autor*in:	Kristy S. Syhapanha [verfasserIn] David A. Russo [verfasserIn] Yun Deng [verfasserIn] Nils Meyer [verfasserIn] Remington X. Poulin [verfasserIn] Georg Pohnert [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics

Übergeordnetes Werk:	In: MicrobiologyOpen - Wiley, 2012, 12(2023), 5, Seite n/a-n/a
Übergeordnetes Werk:	volume:12 ; year:2023 ; number:5 ; pages:n/a-n/a

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1002/mbo3.1387

Katalog-ID:	DOAJ100270263

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245	1	0	\|a Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
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520			\|a Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms.
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653		0	\|a Microbiology
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700	0		\|a Nils Meyer \|e verfasserin \|4 aut
700	0		\|a Remington X. Poulin \|e verfasserin \|4 aut
700	0		\|a Georg Pohnert \|e verfasserin \|4 aut
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912			\|a GBV_ILN_4367
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951			\|a AR
952			\|d 12 \|j 2023 \|e 5 \|h n/a-n/a

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allfields	10.1002/mbo3.1387 doi (DE-627)DOAJ100270263 (DE-599)DOAJ3774136d25f343d3b6a5c90c275dc9de DE-627 ger DE-627 rakwb eng QR1-502 Kristy S. Syhapanha verfasserin aut Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms. algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics Microbiology David A. Russo verfasserin aut Yun Deng verfasserin aut Nils Meyer verfasserin aut Remington X. Poulin verfasserin aut Georg Pohnert verfasserin aut In MicrobiologyOpen Wiley, 2012 12(2023), 5, Seite n/a-n/a (DE-627)718621158 (DE-600)2661368-2 20458827 nnns volume:12 year:2023 number:5 pages:n/a-n/a https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/article/3774136d25f343d3b6a5c90c275dc9de kostenfrei https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/toc/2045-8827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 5 n/a-n/a
spelling	10.1002/mbo3.1387 doi (DE-627)DOAJ100270263 (DE-599)DOAJ3774136d25f343d3b6a5c90c275dc9de DE-627 ger DE-627 rakwb eng QR1-502 Kristy S. Syhapanha verfasserin aut Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms. algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics Microbiology David A. Russo verfasserin aut Yun Deng verfasserin aut Nils Meyer verfasserin aut Remington X. Poulin verfasserin aut Georg Pohnert verfasserin aut In MicrobiologyOpen Wiley, 2012 12(2023), 5, Seite n/a-n/a (DE-627)718621158 (DE-600)2661368-2 20458827 nnns volume:12 year:2023 number:5 pages:n/a-n/a https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/article/3774136d25f343d3b6a5c90c275dc9de kostenfrei https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/toc/2045-8827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 5 n/a-n/a
allfields_unstemmed	10.1002/mbo3.1387 doi (DE-627)DOAJ100270263 (DE-599)DOAJ3774136d25f343d3b6a5c90c275dc9de DE-627 ger DE-627 rakwb eng QR1-502 Kristy S. Syhapanha verfasserin aut Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms. algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics Microbiology David A. Russo verfasserin aut Yun Deng verfasserin aut Nils Meyer verfasserin aut Remington X. Poulin verfasserin aut Georg Pohnert verfasserin aut In MicrobiologyOpen Wiley, 2012 12(2023), 5, Seite n/a-n/a (DE-627)718621158 (DE-600)2661368-2 20458827 nnns volume:12 year:2023 number:5 pages:n/a-n/a https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/article/3774136d25f343d3b6a5c90c275dc9de kostenfrei https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/toc/2045-8827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 5 n/a-n/a
allfieldsGer	10.1002/mbo3.1387 doi (DE-627)DOAJ100270263 (DE-599)DOAJ3774136d25f343d3b6a5c90c275dc9de DE-627 ger DE-627 rakwb eng QR1-502 Kristy S. Syhapanha verfasserin aut Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms. algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics Microbiology David A. Russo verfasserin aut Yun Deng verfasserin aut Nils Meyer verfasserin aut Remington X. Poulin verfasserin aut Georg Pohnert verfasserin aut In MicrobiologyOpen Wiley, 2012 12(2023), 5, Seite n/a-n/a (DE-627)718621158 (DE-600)2661368-2 20458827 nnns volume:12 year:2023 number:5 pages:n/a-n/a https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/article/3774136d25f343d3b6a5c90c275dc9de kostenfrei https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/toc/2045-8827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 5 n/a-n/a
allfieldsSound	10.1002/mbo3.1387 doi (DE-627)DOAJ100270263 (DE-599)DOAJ3774136d25f343d3b6a5c90c275dc9de DE-627 ger DE-627 rakwb eng QR1-502 Kristy S. Syhapanha verfasserin aut Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms. algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics Microbiology David A. Russo verfasserin aut Yun Deng verfasserin aut Nils Meyer verfasserin aut Remington X. Poulin verfasserin aut Georg Pohnert verfasserin aut In MicrobiologyOpen Wiley, 2012 12(2023), 5, Seite n/a-n/a (DE-627)718621158 (DE-600)2661368-2 20458827 nnns volume:12 year:2023 number:5 pages:n/a-n/a https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/article/3774136d25f343d3b6a5c90c275dc9de kostenfrei https://doi.org/10.1002/mbo3.1387 kostenfrei https://doaj.org/toc/2045-8827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2023 5 n/a-n/a
language	English
source	In MicrobiologyOpen 12(2023), 5, Seite n/a-n/a volume:12 year:2023 number:5 pages:n/a-n/a
sourceStr	In MicrobiologyOpen 12(2023), 5, Seite n/a-n/a volume:12 year:2023 number:5 pages:n/a-n/a
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics Microbiology
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container_title	MicrobiologyOpen
authorswithroles_txt_mv	Kristy S. Syhapanha @@aut@@ David A. Russo @@aut@@ Yun Deng @@aut@@ Nils Meyer @@aut@@ Remington X. Poulin @@aut@@ Georg Pohnert @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
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language_de	englisch
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callnumber-first	Q - Science
author	Kristy S. Syhapanha
spellingShingle	Kristy S. Syhapanha misc QR1-502 misc algicidal bacteria misc algicidal protease misc diatoms misc microbial interactions misc phytoplankton misc transcriptomics misc Microbiology Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
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topic_title	QR1-502 Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1 algicidal bacteria algicidal protease diatoms microbial interactions phytoplankton transcriptomics
topic	misc QR1-502 misc algicidal bacteria misc algicidal protease misc diatoms misc microbial interactions misc phytoplankton misc transcriptomics misc Microbiology
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title	Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
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title_full	Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
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author_browse	Kristy S. Syhapanha David A. Russo Yun Deng Nils Meyer Remington X. Poulin Georg Pohnert
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title_sort	transcriptomics‐guided identification of an algicidal protease of the marine bacterium kordia algicida ot‐1
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title_auth	Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
abstract	Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms.
abstractGer	Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms.
abstract_unstemmed	Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. The identification of this algicidal protease opens the possibility of real‐time monitoring of the ecological impact of algicidal bacteria in natural phytoplankton blooms.
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title_short	Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
url	https://doi.org/10.1002/mbo3.1387 https://doaj.org/article/3774136d25f343d3b6a5c90c275dc9de https://doaj.org/toc/2045-8827
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Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients. Kordia algicida strain OT‐1 is a model marine algicidal bacterium that was isolated from a bloom of the diatom Skeletonema costatum. Previous work has suggested that algicidal activity is mediated by secreted proteases. Here, we utilize a transcriptomics‐guided approach to identify the serine protease gene KAOT1_RS09515, hereby named alpA1 as a key element in the algicidal activity of K. algicida. The protease AlpA1 was expressed and purified from a heterologous host and used in in vitro bioassays to validate its activity. We also show that K. algicida is the only algicidal species within a group of four members of the Kordia genus. 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Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1

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