Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations

Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Youssef El Yamlahi [verfasserIn] Naima Bel Mokhtar [verfasserIn] Amal Maurady [verfasserIn] Mohammed R. Britel [verfasserIn] Costas Batargias [verfasserIn] Delphina E. Mutembei [verfasserIn] Hamisi S. Nyingilili [verfasserIn] Deusdedit J. Malulu [verfasserIn] Imna I. Malele [verfasserIn] Elias Asimakis [verfasserIn] Panagiota Stathopoulou [verfasserIn] George Tsiamis [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing

Übergeordnetes Werk:	In: Insects - MDPI AG, 2011, 14(2023), 11, p 840
Übergeordnetes Werk:	volume:14 ; year:2023 ; number:11, p 840

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/insects14110840

Katalog-ID:	DOAJ101232039

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520			\|a Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions.
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allfields	10.3390/insects14110840 doi (DE-627)DOAJ101232039 (DE-599)DOAJ323177c7529e439aa9c68213890f6efc DE-627 ger DE-627 rakwb eng Youssef El Yamlahi verfasserin aut Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions. tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing Science Q Naima Bel Mokhtar verfasserin aut Amal Maurady verfasserin aut Mohammed R. Britel verfasserin aut Costas Batargias verfasserin aut Delphina E. Mutembei verfasserin aut Hamisi S. Nyingilili verfasserin aut Deusdedit J. Malulu verfasserin aut Imna I. Malele verfasserin aut Elias Asimakis verfasserin aut Panagiota Stathopoulou verfasserin aut George Tsiamis verfasserin aut In Insects MDPI AG, 2011 14(2023), 11, p 840 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:11, p 840 https://doi.org/10.3390/insects14110840 kostenfrei https://doaj.org/article/323177c7529e439aa9c68213890f6efc kostenfrei https://www.mdpi.com/2075-4450/14/11/840 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 11, p 840
spelling	10.3390/insects14110840 doi (DE-627)DOAJ101232039 (DE-599)DOAJ323177c7529e439aa9c68213890f6efc DE-627 ger DE-627 rakwb eng Youssef El Yamlahi verfasserin aut Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions. tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing Science Q Naima Bel Mokhtar verfasserin aut Amal Maurady verfasserin aut Mohammed R. Britel verfasserin aut Costas Batargias verfasserin aut Delphina E. Mutembei verfasserin aut Hamisi S. Nyingilili verfasserin aut Deusdedit J. Malulu verfasserin aut Imna I. Malele verfasserin aut Elias Asimakis verfasserin aut Panagiota Stathopoulou verfasserin aut George Tsiamis verfasserin aut In Insects MDPI AG, 2011 14(2023), 11, p 840 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:11, p 840 https://doi.org/10.3390/insects14110840 kostenfrei https://doaj.org/article/323177c7529e439aa9c68213890f6efc kostenfrei https://www.mdpi.com/2075-4450/14/11/840 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 11, p 840
allfields_unstemmed	10.3390/insects14110840 doi (DE-627)DOAJ101232039 (DE-599)DOAJ323177c7529e439aa9c68213890f6efc DE-627 ger DE-627 rakwb eng Youssef El Yamlahi verfasserin aut Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions. tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing Science Q Naima Bel Mokhtar verfasserin aut Amal Maurady verfasserin aut Mohammed R. Britel verfasserin aut Costas Batargias verfasserin aut Delphina E. Mutembei verfasserin aut Hamisi S. Nyingilili verfasserin aut Deusdedit J. Malulu verfasserin aut Imna I. Malele verfasserin aut Elias Asimakis verfasserin aut Panagiota Stathopoulou verfasserin aut George Tsiamis verfasserin aut In Insects MDPI AG, 2011 14(2023), 11, p 840 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:11, p 840 https://doi.org/10.3390/insects14110840 kostenfrei https://doaj.org/article/323177c7529e439aa9c68213890f6efc kostenfrei https://www.mdpi.com/2075-4450/14/11/840 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 11, p 840
allfieldsGer	10.3390/insects14110840 doi (DE-627)DOAJ101232039 (DE-599)DOAJ323177c7529e439aa9c68213890f6efc DE-627 ger DE-627 rakwb eng Youssef El Yamlahi verfasserin aut Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions. tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing Science Q Naima Bel Mokhtar verfasserin aut Amal Maurady verfasserin aut Mohammed R. Britel verfasserin aut Costas Batargias verfasserin aut Delphina E. Mutembei verfasserin aut Hamisi S. Nyingilili verfasserin aut Deusdedit J. Malulu verfasserin aut Imna I. Malele verfasserin aut Elias Asimakis verfasserin aut Panagiota Stathopoulou verfasserin aut George Tsiamis verfasserin aut In Insects MDPI AG, 2011 14(2023), 11, p 840 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:11, p 840 https://doi.org/10.3390/insects14110840 kostenfrei https://doaj.org/article/323177c7529e439aa9c68213890f6efc kostenfrei https://www.mdpi.com/2075-4450/14/11/840 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 11, p 840
allfieldsSound	10.3390/insects14110840 doi (DE-627)DOAJ101232039 (DE-599)DOAJ323177c7529e439aa9c68213890f6efc DE-627 ger DE-627 rakwb eng Youssef El Yamlahi verfasserin aut Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions. tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing Science Q Naima Bel Mokhtar verfasserin aut Amal Maurady verfasserin aut Mohammed R. Britel verfasserin aut Costas Batargias verfasserin aut Delphina E. Mutembei verfasserin aut Hamisi S. Nyingilili verfasserin aut Deusdedit J. Malulu verfasserin aut Imna I. Malele verfasserin aut Elias Asimakis verfasserin aut Panagiota Stathopoulou verfasserin aut George Tsiamis verfasserin aut In Insects MDPI AG, 2011 14(2023), 11, p 840 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:14 year:2023 number:11, p 840 https://doi.org/10.3390/insects14110840 kostenfrei https://doaj.org/article/323177c7529e439aa9c68213890f6efc kostenfrei https://www.mdpi.com/2075-4450/14/11/840 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2023 11, p 840
language	English
source	In Insects 14(2023), 11, p 840 volume:14 year:2023 number:11, p 840
sourceStr	In Insects 14(2023), 11, p 840 volume:14 year:2023 number:11, p 840
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing Science Q
isfreeaccess_bool	true
container_title	Insects
authorswithroles_txt_mv	Youssef El Yamlahi @@aut@@ Naima Bel Mokhtar @@aut@@ Amal Maurady @@aut@@ Mohammed R. Britel @@aut@@ Costas Batargias @@aut@@ Delphina E. Mutembei @@aut@@ Hamisi S. Nyingilili @@aut@@ Deusdedit J. Malulu @@aut@@ Imna I. Malele @@aut@@ Elias Asimakis @@aut@@ Panagiota Stathopoulou @@aut@@ George Tsiamis @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	718627121
id	DOAJ101232039
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">DOAJ101232039</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240414152421.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">240414s2023 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/insects14110840</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ101232039</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ323177c7529e439aa9c68213890f6efc</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Youssef El Yamlahi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. 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author	Youssef El Yamlahi
spellingShingle	Youssef El Yamlahi misc tsetse fly misc bacterial profile misc mass rearing misc 16S rRNA misc amplicon sequencing misc Science misc Q Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations
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topic_title	Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations tsetse fly bacterial profile mass rearing 16S rRNA amplicon sequencing
topic	misc tsetse fly misc bacterial profile misc mass rearing misc 16S rRNA misc amplicon sequencing misc Science misc Q
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title	Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations
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title_full	Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations
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author_browse	Youssef El Yamlahi Naima Bel Mokhtar Amal Maurady Mohammed R. Britel Costas Batargias Delphina E. Mutembei Hamisi S. Nyingilili Deusdedit J. Malulu Imna I. Malele Elias Asimakis Panagiota Stathopoulou George Tsiamis
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author-letter	Youssef El Yamlahi
doi_str_mv	10.3390/insects14110840
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title_sort	characterization of the bacterial profile from natural and laboratory <i<glossina</i< populations
title_auth	Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations
abstract	Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions.
abstractGer	Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions.
abstract_unstemmed	Tsetse flies (<i<Glossina</i< spp.; Diptera: Glossinidae) are viviparous flies that feed on blood and are found exclusively in sub-Saharan Africa. They are the only cyclic vectors of African trypanosomes, responsible for human African trypanosomiasis (HAT) and animal African trypanosomiasis (AAT). In this study, we employed high throughput sequencing of the 16S rRNA gene to unravel the diversity of symbiotic bacteria in five wild and three laboratory populations of tsetse species (<i<Glossina pallidipes</i<, <i<G. morsitans</i<, <i<G. swynnertoni</i<, and <i<G. austeni</i<). The aim was to assess the dynamics of bacterial diversity both within each laboratory and wild population in relation to the developmental stage, insect age, gender, and location. Our results indicated that the bacterial communities associated with the four studied <i<Glossina</i< species were significantly influenced by their region of origin, with wild samples being more diverse compared to the laboratory samples. We also observed that the larval microbiota was significantly different than the adults. Furthermore, the sex and the species did not significantly influence the formation of the bacterial profile of the laboratory colonies once these populations were kept under the same rearing conditions. In addition, <i<Wigglesworthia</i<, <i<Acinetobacter</i<, and <i<Sodalis</i< were the most abundant bacterial genera in all the samples, while <i<Wolbachia</i< was significantly abundant in <i<G. morsitans</i< compared to the other studied species. The operational taxonomic unit (OTU) co-occurrence network for each location (VVBD insectary, Doma, Makao, and Msubugwe) indicated a high variability between <i<G. pallidipes</i< and the other species in terms of the number of mutual exclusion and copresence interactions. In particular, some bacterial genera, like <i<Wigglesworthia</i< and <i<Sodalis</i<, with high relative abundance, were also characterized by a high degree of interactions.
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title_short	Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations
url	https://doi.org/10.3390/insects14110840 https://doaj.org/article/323177c7529e439aa9c68213890f6efc https://www.mdpi.com/2075-4450/14/11/840 https://doaj.org/toc/2075-4450
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author2Str	Naima Bel Mokhtar Amal Maurady Mohammed R. Britel Costas Batargias Delphina E. Mutembei Hamisi S. Nyingilili Deusdedit J. Malulu Imna I. Malele Elias Asimakis Panagiota Stathopoulou George Tsiamis
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Characterization of the Bacterial Profile from Natural and Laboratory <i<Glossina</i< Populations

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