OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.)

Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the sp...
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Autor*in:	Shengyuan Guo [verfasserIn] Chuanjiang Zheng [verfasserIn] Yan Wang [verfasserIn] Yangwen Xu [verfasserIn] Jinwen Wu [verfasserIn] Lan Wang [verfasserIn] Xiangdong Liu [verfasserIn] Zhixiong Chen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	rice miRNA seed-setting rate embryo sac IAA

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 24(2023), 22, p 16240
Übergeordnetes Werk:	volume:24 ; year:2023 ; number:22, p 16240

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms242216240

Katalog-ID:	DOAJ101235992

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allfields	10.3390/ijms242216240 doi (DE-627)DOAJ101235992 (DE-599)DOAJ8d9b597c4e074d188c8eedcc52458eb3 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Shengyuan Guo verfasserin aut OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<. rice miRNA seed-setting rate embryo sac IAA Biology (General) Chemistry Chuanjiang Zheng verfasserin aut Yan Wang verfasserin aut Yangwen Xu verfasserin aut Jinwen Wu verfasserin aut Lan Wang verfasserin aut Xiangdong Liu verfasserin aut Zhixiong Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 22, p 16240 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:22, p 16240 https://doi.org/10.3390/ijms242216240 kostenfrei https://doaj.org/article/8d9b597c4e074d188c8eedcc52458eb3 kostenfrei https://www.mdpi.com/1422-0067/24/22/16240 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 22, p 16240
spelling	10.3390/ijms242216240 doi (DE-627)DOAJ101235992 (DE-599)DOAJ8d9b597c4e074d188c8eedcc52458eb3 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Shengyuan Guo verfasserin aut OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<. rice miRNA seed-setting rate embryo sac IAA Biology (General) Chemistry Chuanjiang Zheng verfasserin aut Yan Wang verfasserin aut Yangwen Xu verfasserin aut Jinwen Wu verfasserin aut Lan Wang verfasserin aut Xiangdong Liu verfasserin aut Zhixiong Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 22, p 16240 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:22, p 16240 https://doi.org/10.3390/ijms242216240 kostenfrei https://doaj.org/article/8d9b597c4e074d188c8eedcc52458eb3 kostenfrei https://www.mdpi.com/1422-0067/24/22/16240 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 22, p 16240
allfields_unstemmed	10.3390/ijms242216240 doi (DE-627)DOAJ101235992 (DE-599)DOAJ8d9b597c4e074d188c8eedcc52458eb3 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Shengyuan Guo verfasserin aut OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<. rice miRNA seed-setting rate embryo sac IAA Biology (General) Chemistry Chuanjiang Zheng verfasserin aut Yan Wang verfasserin aut Yangwen Xu verfasserin aut Jinwen Wu verfasserin aut Lan Wang verfasserin aut Xiangdong Liu verfasserin aut Zhixiong Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 22, p 16240 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:22, p 16240 https://doi.org/10.3390/ijms242216240 kostenfrei https://doaj.org/article/8d9b597c4e074d188c8eedcc52458eb3 kostenfrei https://www.mdpi.com/1422-0067/24/22/16240 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 22, p 16240
allfieldsGer	10.3390/ijms242216240 doi (DE-627)DOAJ101235992 (DE-599)DOAJ8d9b597c4e074d188c8eedcc52458eb3 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Shengyuan Guo verfasserin aut OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<. rice miRNA seed-setting rate embryo sac IAA Biology (General) Chemistry Chuanjiang Zheng verfasserin aut Yan Wang verfasserin aut Yangwen Xu verfasserin aut Jinwen Wu verfasserin aut Lan Wang verfasserin aut Xiangdong Liu verfasserin aut Zhixiong Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 22, p 16240 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:22, p 16240 https://doi.org/10.3390/ijms242216240 kostenfrei https://doaj.org/article/8d9b597c4e074d188c8eedcc52458eb3 kostenfrei https://www.mdpi.com/1422-0067/24/22/16240 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 22, p 16240
allfieldsSound	10.3390/ijms242216240 doi (DE-627)DOAJ101235992 (DE-599)DOAJ8d9b597c4e074d188c8eedcc52458eb3 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Shengyuan Guo verfasserin aut OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.) 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<. rice miRNA seed-setting rate embryo sac IAA Biology (General) Chemistry Chuanjiang Zheng verfasserin aut Yan Wang verfasserin aut Yangwen Xu verfasserin aut Jinwen Wu verfasserin aut Lan Wang verfasserin aut Xiangdong Liu verfasserin aut Zhixiong Chen verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 24(2023), 22, p 16240 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:24 year:2023 number:22, p 16240 https://doi.org/10.3390/ijms242216240 kostenfrei https://doaj.org/article/8d9b597c4e074d188c8eedcc52458eb3 kostenfrei https://www.mdpi.com/1422-0067/24/22/16240 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2023 22, p 16240
language	English
source	In International Journal of Molecular Sciences 24(2023), 22, p 16240 volume:24 year:2023 number:22, p 16240
sourceStr	In International Journal of Molecular Sciences 24(2023), 22, p 16240 volume:24 year:2023 number:22, p 16240
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	rice miRNA seed-setting rate embryo sac IAA Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Shengyuan Guo @@aut@@ Chuanjiang Zheng @@aut@@ Yan Wang @@aut@@ Yangwen Xu @@aut@@ Jinwen Wu @@aut@@ Lan Wang @@aut@@ Xiangdong Liu @@aut@@ Zhixiong Chen @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ101235992
language_de	englisch
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callnumber-first	Q - Science
author	Shengyuan Guo
spellingShingle	Shengyuan Guo misc QH301-705.5 misc QD1-999 misc rice misc miRNA misc seed-setting rate misc embryo sac misc IAA misc Biology (General) misc Chemistry OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.)
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topic_title	QH301-705.5 QD1-999 OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.) rice miRNA seed-setting rate embryo sac IAA
topic	misc QH301-705.5 misc QD1-999 misc rice misc miRNA misc seed-setting rate misc embryo sac misc IAA misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc rice misc miRNA misc seed-setting rate misc embryo sac misc IAA misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc rice misc miRNA misc seed-setting rate misc embryo sac misc IAA misc Biology (General) misc Chemistry
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title	OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.)
ctrlnum	(DE-627)DOAJ101235992 (DE-599)DOAJ8d9b597c4e074d188c8eedcc52458eb3
title_full	OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.)
author_sort	Shengyuan Guo
journal	International Journal of Molecular Sciences
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author_browse	Shengyuan Guo Chuanjiang Zheng Yan Wang Yangwen Xu Jinwen Wu Lan Wang Xiangdong Liu Zhixiong Chen
container_volume	24
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format_se	Elektronische Aufsätze
author-letter	Shengyuan Guo
doi_str_mv	10.3390/ijms242216240
author2-role	verfasserin
title_sort	osmirna5488 regulates the development of embryo sacs and targets <i<osarf25</i< in rice (<i<oryza sativa</i< l.)
callnumber	QH301-705.5
title_auth	OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.)
abstract	Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<.
abstractGer	Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<.
abstract_unstemmed	Small RNAs are a class of non-coding RNAs that typically range from 20 to 24 nucleotides in length. Among them, microRNAs (miRNAs) are particularly important regulators for plant development. The biological function of the conserved miRNAs has been studied extensively in plants, while that of the species-specific miRNAs has been studied in-depth. In this study, the regulatory role of a rice-specific OsmiRNA5488 (OsmiR5488) was characterized with the miR5488-overexpressed line (<i<miR5488-OE</i<) and miR5488-silenced line (<i<STTM-5488</i<). The seed-setting rate was notably reduced in <i<miR5488-OE</i< lines, but not in <i<STTM-5488</i< lines. Cytological observation demonstrated the different types of abnormal mature embryo sacs, including the degeneration of embryo sacs and other variant types, in <i<miR5488-OE</i< lines. The percentage of the abnormal mature embryo sacs accounted for the reduced value of the seed-setting rate. Furthermore, <i<OsARF25</i< was identified as a target of <i<OsmiR5488</i< via RNA ligase-mediated 3′-amplifification of cDNA ends, dual luciferase assays, and transient expression assays. The primary root length was decreased with the increases in auxin concentrations in <i<miR5488-OE</i< lines compared to wild-type rice. Summarily, our results suggested that OsmiR5488 regulates the seed-setting rate and down-regulates the targeted gene <i<OsARF25</i<.
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container_issue	22, p 16240
title_short	OsmiRNA5488 Regulates the Development of Embryo Sacs and Targets <i<OsARF25</i< in Rice (<i<Oryza sativa</i< L.)
url	https://doi.org/10.3390/ijms242216240 https://doaj.org/article/8d9b597c4e074d188c8eedcc52458eb3 https://www.mdpi.com/1422-0067/24/22/16240 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Chuanjiang Zheng Yan Wang Yangwen Xu Jinwen Wu Lan Wang Xiangdong Liu Zhixiong Chen
author2Str	Chuanjiang Zheng Yan Wang Yangwen Xu Jinwen Wu Lan Wang Xiangdong Liu Zhixiong Chen
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doi_str	10.3390/ijms242216240
callnumber-a	QH301-705.5
up_date	2024-07-03T19:28:25.874Z
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