Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria

For the rapid detection of bacteria in a blood sample, nucleic acid amplification-based assays are believed to be promising. Nevertheless, the nucleic acids released from the dead blood cells or bacteria could affect the assay performance. This highlights the importance of the isolation of live bact...
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Autor*in:	Po-Yu Chu [verfasserIn] Chia-Ming Yang [verfasserIn] Kai-Lin Huang [verfasserIn] Ai-Yun Wu [verfasserIn] Chia-Hsun Hsieh [verfasserIn] A-Ching Chao [verfasserIn] Min-Hsien Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	optically induced dielectrophoresis (ODEP) microfluidic system bacteria isolation purification

Übergeordnetes Werk:	In: Biosensors - MDPI AG, 2012, 13(2023), 11, p 952
Übergeordnetes Werk:	volume:13 ; year:2023 ; number:11, p 952

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/bios13110952

Katalog-ID:	DOAJ101268165

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authorswithroles_txt_mv	Po-Yu Chu @@aut@@ Chia-Ming Yang @@aut@@ Kai-Lin Huang @@aut@@ Ai-Yun Wu @@aut@@ Chia-Hsun Hsieh @@aut@@ A-Ching Chao @@aut@@ Min-Hsien Wu @@aut@@
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callnumber-first	T - Technology
author	Po-Yu Chu
spellingShingle	Po-Yu Chu misc TP248.13-248.65 misc optically induced dielectrophoresis (ODEP) misc microfluidic system misc bacteria misc isolation misc purification misc Biotechnology Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria
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topic_title	TP248.13-248.65 Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria optically induced dielectrophoresis (ODEP) microfluidic system bacteria isolation purification
topic	misc TP248.13-248.65 misc optically induced dielectrophoresis (ODEP) misc microfluidic system misc bacteria misc isolation misc purification misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc optically induced dielectrophoresis (ODEP) misc microfluidic system misc bacteria misc isolation misc purification misc Biotechnology
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title	Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria
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title_full	Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria
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author_browse	Po-Yu Chu Chia-Ming Yang Kai-Lin Huang Ai-Yun Wu Chia-Hsun Hsieh A-Ching Chao Min-Hsien Wu
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title_sort	development of an optically induced dielectrophoresis (odep) microfluidic system for high-performance isolation and purification of bacteria
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title_auth	Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria
abstract	For the rapid detection of bacteria in a blood sample, nucleic acid amplification-based assays are believed to be promising. Nevertheless, the nucleic acids released from the dead blood cells or bacteria could affect the assay performance. This highlights the importance of the isolation of live bacteria from blood samples. To address this issue, this study proposes a two-step process. First, a blood sample was treated with the immuno-magnetic microbeads-based separation to remove the majority of blood cells. Second, an optically induced dielectrophoresis (ODEP) microfluidic system with an integrated dynamic circular light image array was utilized to further isolate and purify the live bacteria from the remaining blood cells based on their size difference. In this work, the ODEP microfluidic system was developed. Its performance for the isolation and purification of bacteria was evaluated. The results revealed that the method was able to harvest the live bacteria in a high purity (90.5~99.2%) manner. Overall, the proposed method was proven to be capable of isolating and purifying high-purity live bacteria without causing damage to the co-existing cells. This technical feature was found to be valuable for the subsequent nucleic-acid-based bacteria detection, in which the interferences caused by the nontarget nucleic acids could be eliminated.
abstractGer	For the rapid detection of bacteria in a blood sample, nucleic acid amplification-based assays are believed to be promising. Nevertheless, the nucleic acids released from the dead blood cells or bacteria could affect the assay performance. This highlights the importance of the isolation of live bacteria from blood samples. To address this issue, this study proposes a two-step process. First, a blood sample was treated with the immuno-magnetic microbeads-based separation to remove the majority of blood cells. Second, an optically induced dielectrophoresis (ODEP) microfluidic system with an integrated dynamic circular light image array was utilized to further isolate and purify the live bacteria from the remaining blood cells based on their size difference. In this work, the ODEP microfluidic system was developed. Its performance for the isolation and purification of bacteria was evaluated. The results revealed that the method was able to harvest the live bacteria in a high purity (90.5~99.2%) manner. Overall, the proposed method was proven to be capable of isolating and purifying high-purity live bacteria without causing damage to the co-existing cells. This technical feature was found to be valuable for the subsequent nucleic-acid-based bacteria detection, in which the interferences caused by the nontarget nucleic acids could be eliminated.
abstract_unstemmed	For the rapid detection of bacteria in a blood sample, nucleic acid amplification-based assays are believed to be promising. Nevertheless, the nucleic acids released from the dead blood cells or bacteria could affect the assay performance. This highlights the importance of the isolation of live bacteria from blood samples. To address this issue, this study proposes a two-step process. First, a blood sample was treated with the immuno-magnetic microbeads-based separation to remove the majority of blood cells. Second, an optically induced dielectrophoresis (ODEP) microfluidic system with an integrated dynamic circular light image array was utilized to further isolate and purify the live bacteria from the remaining blood cells based on their size difference. In this work, the ODEP microfluidic system was developed. Its performance for the isolation and purification of bacteria was evaluated. The results revealed that the method was able to harvest the live bacteria in a high purity (90.5~99.2%) manner. Overall, the proposed method was proven to be capable of isolating and purifying high-purity live bacteria without causing damage to the co-existing cells. This technical feature was found to be valuable for the subsequent nucleic-acid-based bacteria detection, in which the interferences caused by the nontarget nucleic acids could be eliminated.
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title_short	Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria
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Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria

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