The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro
The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as tradition...
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Maya Sari [verfasserIn] Mayta Novaliza Isda [verfasserIn] |
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| Sprache: |
Indonesisch |
| Erschienen: |
2021 |
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In: Jurnal Biologi Universitas Andalas - Jurusan Biologi Universitas Andalas, 2019, 9(2021), 1, Seite 8-17 |
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volume:9 ; year:2021 ; number:1 ; pages:8-17 |
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| DOI / URN: |
10.25077/jbioua.9.1.8-17.2021 |
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| 520 | |a The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. | ||
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10.25077/jbioua.9.1.8-17.2021 doi (DE-627)DOAJ101291035 (DE-599)DOAJ189d783f53f94d5b87c7b816dabc9c74 DE-627 ger DE-627 rakwb ind QR1-502 Maya Sari verfasserin aut The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. Microbiology Mayta Novaliza Isda verfasserin aut In Jurnal Biologi Universitas Andalas Jurusan Biologi Universitas Andalas, 2019 9(2021), 1, Seite 8-17 (DE-627)1760603384 26559587 nnns volume:9 year:2021 number:1 pages:8-17 https://doi.org/10.25077/jbioua.9.1.8-17.2021 kostenfrei https://doaj.org/article/189d783f53f94d5b87c7b816dabc9c74 kostenfrei http://jbioua.fmipa.unand.ac.id/index.php/jbioua/article/view/337 kostenfrei https://doaj.org/toc/2303-2162 Journal toc kostenfrei https://doaj.org/toc/2655-9587 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 9 2021 1 8-17 |
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10.25077/jbioua.9.1.8-17.2021 doi (DE-627)DOAJ101291035 (DE-599)DOAJ189d783f53f94d5b87c7b816dabc9c74 DE-627 ger DE-627 rakwb ind QR1-502 Maya Sari verfasserin aut The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. Microbiology Mayta Novaliza Isda verfasserin aut In Jurnal Biologi Universitas Andalas Jurusan Biologi Universitas Andalas, 2019 9(2021), 1, Seite 8-17 (DE-627)1760603384 26559587 nnns volume:9 year:2021 number:1 pages:8-17 https://doi.org/10.25077/jbioua.9.1.8-17.2021 kostenfrei https://doaj.org/article/189d783f53f94d5b87c7b816dabc9c74 kostenfrei http://jbioua.fmipa.unand.ac.id/index.php/jbioua/article/view/337 kostenfrei https://doaj.org/toc/2303-2162 Journal toc kostenfrei https://doaj.org/toc/2655-9587 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 9 2021 1 8-17 |
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10.25077/jbioua.9.1.8-17.2021 doi (DE-627)DOAJ101291035 (DE-599)DOAJ189d783f53f94d5b87c7b816dabc9c74 DE-627 ger DE-627 rakwb ind QR1-502 Maya Sari verfasserin aut The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. Microbiology Mayta Novaliza Isda verfasserin aut In Jurnal Biologi Universitas Andalas Jurusan Biologi Universitas Andalas, 2019 9(2021), 1, Seite 8-17 (DE-627)1760603384 26559587 nnns volume:9 year:2021 number:1 pages:8-17 https://doi.org/10.25077/jbioua.9.1.8-17.2021 kostenfrei https://doaj.org/article/189d783f53f94d5b87c7b816dabc9c74 kostenfrei http://jbioua.fmipa.unand.ac.id/index.php/jbioua/article/view/337 kostenfrei https://doaj.org/toc/2303-2162 Journal toc kostenfrei https://doaj.org/toc/2655-9587 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 9 2021 1 8-17 |
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10.25077/jbioua.9.1.8-17.2021 doi (DE-627)DOAJ101291035 (DE-599)DOAJ189d783f53f94d5b87c7b816dabc9c74 DE-627 ger DE-627 rakwb ind QR1-502 Maya Sari verfasserin aut The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. Microbiology Mayta Novaliza Isda verfasserin aut In Jurnal Biologi Universitas Andalas Jurusan Biologi Universitas Andalas, 2019 9(2021), 1, Seite 8-17 (DE-627)1760603384 26559587 nnns volume:9 year:2021 number:1 pages:8-17 https://doi.org/10.25077/jbioua.9.1.8-17.2021 kostenfrei https://doaj.org/article/189d783f53f94d5b87c7b816dabc9c74 kostenfrei http://jbioua.fmipa.unand.ac.id/index.php/jbioua/article/view/337 kostenfrei https://doaj.org/toc/2303-2162 Journal toc kostenfrei https://doaj.org/toc/2655-9587 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 9 2021 1 8-17 |
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Elektronische Aufsätze |
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Maya Sari |
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10.25077/jbioua.9.1.8-17.2021 |
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response of callus formation from tacca chantrieri leaves with various concentrations of 2,4-d and bap by in vitro |
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QR1-502 |
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The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro |
| abstract |
The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. |
| abstractGer |
The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. |
| abstract_unstemmed |
The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting. |
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The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro |
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https://doi.org/10.25077/jbioua.9.1.8-17.2021 https://doaj.org/article/189d783f53f94d5b87c7b816dabc9c74 http://jbioua.fmipa.unand.ac.id/index.php/jbioua/article/view/337 https://doaj.org/toc/2303-2162 https://doaj.org/toc/2655-9587 |
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Mayta Novaliza Isda |
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2024-07-03T19:49:48.003Z |
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