Advances in the axenic isolation methods of Blastocystis sp. and their applications

Blastocystis sp. is a prevalent protistan parasite found globally in the gastrointestinal tract of humans and various animals. This review aims to elucidate the advancements in research on axenic isolation techniques for Blastocystis sp. and their diverse applications. Axenic isolation, involving th...
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Autor*in:	Xuefang Mei [verfasserIn] Lai Wei [verfasserIn] Changwei Su [verfasserIn] Zhenke Yang [verfasserIn] Xiaowei Tian [verfasserIn] Zhenchao Zhang [verfasserIn] Shuai Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	antibiotics axenic isolation Blastocystis sp influencing factors medium

Übergeordnetes Werk:	In: Parasitology - Cambridge University Press, 2023, 151(2024), Seite 125-134
Übergeordnetes Werk:	volume:151 ; year:2024 ; pages:125-134

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1017/S0031182023001300

Katalog-ID:	DOAJ101442122

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author	Xuefang Mei
spellingShingle	Xuefang Mei misc QD415-436 misc RC109-216 misc QR1-502 misc antibiotics misc axenic isolation misc Blastocystis sp misc influencing factors misc medium misc Biochemistry misc Infectious and parasitic diseases misc Microbiology Advances in the axenic isolation methods of Blastocystis sp. and their applications
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topic_title	QD415-436 RC109-216 QR1-502 Advances in the axenic isolation methods of Blastocystis sp. and their applications antibiotics axenic isolation Blastocystis sp influencing factors medium
topic	misc QD415-436 misc RC109-216 misc QR1-502 misc antibiotics misc axenic isolation misc Blastocystis sp misc influencing factors misc medium misc Biochemistry misc Infectious and parasitic diseases misc Microbiology
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title	Advances in the axenic isolation methods of Blastocystis sp. and their applications
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title_full	Advances in the axenic isolation methods of Blastocystis sp. and their applications
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title_sort	advances in the axenic isolation methods of blastocystis sp. and their applications
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title_auth	Advances in the axenic isolation methods of Blastocystis sp. and their applications
abstract	Blastocystis sp. is a prevalent protistan parasite found globally in the gastrointestinal tract of humans and various animals. This review aims to elucidate the advancements in research on axenic isolation techniques for Blastocystis sp. and their diverse applications. Axenic isolation, involving the culture and isolation of Blastocystis sp. free from any other organisms, necessitates the application of specific media and a series of axenic treatment methods. These methods encompass antibiotic treatment, monoclonal culture, differential centrifugation, density gradient separation, micromanipulation and the combined use of culture media. Critical factors influencing axenic isolation effectiveness include medium composition, culture temperature, medium characteristics, antibiotic type and dosage and the subtype (ST) of Blastocystis sp. Applications of axenic isolation encompass exploring pathogenicity, karyotype and ST analysis, immunoassay, characterization of surface chemical structure and lipid composition and understanding drug treatment effects. This review serves as a valuable reference for clinicians and scientists in selecting appropriate axenic isolation methods.
abstractGer	Blastocystis sp. is a prevalent protistan parasite found globally in the gastrointestinal tract of humans and various animals. This review aims to elucidate the advancements in research on axenic isolation techniques for Blastocystis sp. and their diverse applications. Axenic isolation, involving the culture and isolation of Blastocystis sp. free from any other organisms, necessitates the application of specific media and a series of axenic treatment methods. These methods encompass antibiotic treatment, monoclonal culture, differential centrifugation, density gradient separation, micromanipulation and the combined use of culture media. Critical factors influencing axenic isolation effectiveness include medium composition, culture temperature, medium characteristics, antibiotic type and dosage and the subtype (ST) of Blastocystis sp. Applications of axenic isolation encompass exploring pathogenicity, karyotype and ST analysis, immunoassay, characterization of surface chemical structure and lipid composition and understanding drug treatment effects. This review serves as a valuable reference for clinicians and scientists in selecting appropriate axenic isolation methods.
abstract_unstemmed	Blastocystis sp. is a prevalent protistan parasite found globally in the gastrointestinal tract of humans and various animals. This review aims to elucidate the advancements in research on axenic isolation techniques for Blastocystis sp. and their diverse applications. Axenic isolation, involving the culture and isolation of Blastocystis sp. free from any other organisms, necessitates the application of specific media and a series of axenic treatment methods. These methods encompass antibiotic treatment, monoclonal culture, differential centrifugation, density gradient separation, micromanipulation and the combined use of culture media. Critical factors influencing axenic isolation effectiveness include medium composition, culture temperature, medium characteristics, antibiotic type and dosage and the subtype (ST) of Blastocystis sp. Applications of axenic isolation encompass exploring pathogenicity, karyotype and ST analysis, immunoassay, characterization of surface chemical structure and lipid composition and understanding drug treatment effects. This review serves as a valuable reference for clinicians and scientists in selecting appropriate axenic isolation methods.
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title_short	Advances in the axenic isolation methods of Blastocystis sp. and their applications
url	https://doi.org/10.1017/S0031182023001300 https://doaj.org/article/800126fb19d34e0882b46b4c341bdc08 https://www.cambridge.org/core/product/identifier/S0031182023001300/type/journal_article https://doaj.org/toc/0031-1820 https://doaj.org/toc/1469-8161
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up_date	2024-07-03T20:39:14.113Z
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Advances in the axenic isolation methods of Blastocystis sp. and their applications

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