Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry

Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of...
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Gespeichert in:

Autor*in:	Konášová, Renáta [verfasserIn] Koval, Dušan [verfasserIn] Dytrtová, Jana Jaklová [verfasserIn] Kašička, Václav [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant

Übergeordnetes Werk:	Enthalten in: Journal of chromatography / A - New York, NY [u.a.] : Science Direct, 1958, 1568
Übergeordnetes Werk:	volume:1568

DOI / URN:	10.1016/j.chroma.2018.07.014

Katalog-ID:	ELV000259446

Internformat


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245	1	0	\|a Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry
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520			\|a Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method.
650		4	\|a Affinity capillary electrophoresis
650		4	\|a CE-MS interface
650		4	\|a Crown ether complexes
650		4	\|a Electrospray ionization
650		4	\|a Mass spectrometry
650		4	\|a Stability constant
700	1		\|a Koval, Dušan \|e verfasserin \|4 aut
700	1		\|a Dytrtová, Jana Jaklová \|e verfasserin \|4 aut
700	1		\|a Kašička, Václav \|e verfasserin \|0 (orcid)0000-0003-1719-1432 \|4 aut
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912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
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912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
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912			\|a GBV_ILN_2049
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912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
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912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 35.00 \|j Chemie: Allgemeines
951			\|a AR
952			\|d 1568

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matchkey_str	article:18733778:2018----::oprsnfwlwlwnefcsomaueetfoiiisnsaiiyosatbafntcpla
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publishDate	2018
allfields	10.1016/j.chroma.2018.07.014 doi (DE-627)ELV000259446 (ELSEVIER)S0021-9673(18)30856-2 DE-627 ger DE-627 rda eng 540 DE-600 35.00 bkl Konášová, Renáta verfasserin aut Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method. Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant Koval, Dušan verfasserin aut Dytrtová, Jana Jaklová verfasserin aut Kašička, Václav verfasserin (orcid)0000-0003-1719-1432 aut Enthalten in Journal of chromatography / A New York, NY [u.a.] : Science Direct, 1958 1568 Online-Ressource (DE-627)302467416 (DE-600)1491247-8 (DE-576)094950253 1873-3778 nnns volume:1568 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines AR 1568
spelling	10.1016/j.chroma.2018.07.014 doi (DE-627)ELV000259446 (ELSEVIER)S0021-9673(18)30856-2 DE-627 ger DE-627 rda eng 540 DE-600 35.00 bkl Konášová, Renáta verfasserin aut Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method. Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant Koval, Dušan verfasserin aut Dytrtová, Jana Jaklová verfasserin aut Kašička, Václav verfasserin (orcid)0000-0003-1719-1432 aut Enthalten in Journal of chromatography / A New York, NY [u.a.] : Science Direct, 1958 1568 Online-Ressource (DE-627)302467416 (DE-600)1491247-8 (DE-576)094950253 1873-3778 nnns volume:1568 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines AR 1568
allfields_unstemmed	10.1016/j.chroma.2018.07.014 doi (DE-627)ELV000259446 (ELSEVIER)S0021-9673(18)30856-2 DE-627 ger DE-627 rda eng 540 DE-600 35.00 bkl Konášová, Renáta verfasserin aut Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method. Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant Koval, Dušan verfasserin aut Dytrtová, Jana Jaklová verfasserin aut Kašička, Václav verfasserin (orcid)0000-0003-1719-1432 aut Enthalten in Journal of chromatography / A New York, NY [u.a.] : Science Direct, 1958 1568 Online-Ressource (DE-627)302467416 (DE-600)1491247-8 (DE-576)094950253 1873-3778 nnns volume:1568 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines AR 1568
allfieldsGer	10.1016/j.chroma.2018.07.014 doi (DE-627)ELV000259446 (ELSEVIER)S0021-9673(18)30856-2 DE-627 ger DE-627 rda eng 540 DE-600 35.00 bkl Konášová, Renáta verfasserin aut Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method. Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant Koval, Dušan verfasserin aut Dytrtová, Jana Jaklová verfasserin aut Kašička, Václav verfasserin (orcid)0000-0003-1719-1432 aut Enthalten in Journal of chromatography / A New York, NY [u.a.] : Science Direct, 1958 1568 Online-Ressource (DE-627)302467416 (DE-600)1491247-8 (DE-576)094950253 1873-3778 nnns volume:1568 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines AR 1568
allfieldsSound	10.1016/j.chroma.2018.07.014 doi (DE-627)ELV000259446 (ELSEVIER)S0021-9673(18)30856-2 DE-627 ger DE-627 rda eng 540 DE-600 35.00 bkl Konášová, Renáta verfasserin aut Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method. Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant Koval, Dušan verfasserin aut Dytrtová, Jana Jaklová verfasserin aut Kašička, Václav verfasserin (orcid)0000-0003-1719-1432 aut Enthalten in Journal of chromatography / A New York, NY [u.a.] : Science Direct, 1958 1568 Online-Ressource (DE-627)302467416 (DE-600)1491247-8 (DE-576)094950253 1873-3778 nnns volume:1568 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines AR 1568
language	English
source	Enthalten in Journal of chromatography / A 1568 volume:1568
sourceStr	Enthalten in Journal of chromatography / A 1568 volume:1568
format_phy_str_mv	Article
bklname	Chemie: Allgemeines
institution	findex.gbv.de
topic_facet	Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant
dewey-raw	540
isfreeaccess_bool	false
container_title	Journal of chromatography / A
authorswithroles_txt_mv	Konášová, Renáta @@aut@@ Koval, Dušan @@aut@@ Dytrtová, Jana Jaklová @@aut@@ Kašička, Václav @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	302467416
dewey-sort	3540
id	ELV000259446
language_de	englisch
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author	Konášová, Renáta
spellingShingle	Konášová, Renáta ddc 540 bkl 35.00 misc Affinity capillary electrophoresis misc CE-MS interface misc Crown ether complexes misc Electrospray ionization misc Mass spectrometry misc Stability constant Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry
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topic_title	540 DE-600 35.00 bkl Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry Affinity capillary electrophoresis CE-MS interface Crown ether complexes Electrospray ionization Mass spectrometry Stability constant
topic	ddc 540 bkl 35.00 misc Affinity capillary electrophoresis misc CE-MS interface misc Crown ether complexes misc Electrospray ionization misc Mass spectrometry misc Stability constant
topic_unstemmed	ddc 540 bkl 35.00 misc Affinity capillary electrophoresis misc CE-MS interface misc Crown ether complexes misc Electrospray ionization misc Mass spectrometry misc Stability constant
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title	Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry
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title_full	Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry
author_sort	Konášová, Renáta
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author_browse	Konášová, Renáta Koval, Dušan Dytrtová, Jana Jaklová Kašička, Václav
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author-letter	Konášová, Renáta
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title_sort	comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry
title_auth	Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry
abstract	Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method.
abstractGer	Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method.
abstract_unstemmed	Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, K st, of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of K st of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system. The effective mobilities of the crown ether ligands and the K st of their potassium complexes were measured/determined by ACE-ESI/MS using two lab-made interfaces: (i) a sheathless porous tip CE-ESI/MS interface and (ii) a nano-sheath liquid flow CE-ESI/MS interface, and, in turn, compared with those obtained by ACE with UV spectrophotometric detection. Apparent stability constant of potassium-crown ether complexes in 60/40 (v/v) methanol/water mixed solvent, pH* 5.5, was about 1300 L/mol for dibenzo-18-crown-6, 1600 L/mol for benzo-18-crown-6 and 5200 L/mol for 18-crown-6 ligands, respectively. It was observed that electrophoretic mobilities from CE-MS experiments differ from reference values determined by UV detection by ∼7% depending on the CE-MS interface used. Good agreement of CE-MS and CE-UV data was achieved for nano-sheath liquid flow interface, in which the spray potential and the CE separation potential can be effectively decoupled. As for sheathless porous tip interface, a correction procedure involving a mobility marker has been proposed. It provides typically only ca. 1% difference of effective mobilities and K st values obtained from CE-MS data as compared to those received by the reference ACE-UV method.
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title_short	Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry
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author2	Koval, Dušan Dytrtová, Jana Jaklová Kašička, Václav
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doi_str	10.1016/j.chroma.2018.07.014
up_date	2024-07-06T17:22:05.356Z
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Comparison of two low flow interfaces for measurement of mobilities and stability constants by affinity capillary electrophoresis–mass spectrometry

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