Concerns regarding ‘off-target’ activity of genome editing endonucleases

Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced sh...
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Gespeichert in:

Autor*in:	Kadam, Ulhas Sopanrao [verfasserIn] Shelake, Rahul Mahadev [verfasserIn] Chavhan, Rahul L. [verfasserIn] Suprasanna, Penna [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases

Übergeordnetes Werk:	Enthalten in: Plant physiology and biochemistry - Amsterdam [u.a.] : Elsevier Science, 1998, 131, Seite 22-30
Übergeordnetes Werk:	volume:131 ; pages:22-30

DOI / URN:	10.1016/j.plaphy.2018.03.027

Katalog-ID:	ELV000346675

Internformat


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100	1		\|a Kadam, Ulhas Sopanrao \|e verfasserin \|0 (orcid)0000-0001-8737-167X \|4 aut
245	1	0	\|a Concerns regarding ‘off-target’ activity of genome editing endonucleases
264		1	\|c 2018
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337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement.
650		4	\|a Genome editing
650		4	\|a Engineered or synthetic nucleases
650		4	\|a Zinc Finger Nucleases (ZFNs)
650		4	\|a Transcription Activator Effector-Like Nucleases (TALENs)
650		4	\|a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)
650		4	\|a Endonucleases
700	1		\|a Shelake, Rahul Mahadev \|e verfasserin \|0 (orcid)0000-0003-0691-560X \|4 aut
700	1		\|a Chavhan, Rahul L. \|e verfasserin \|4 aut
700	1		\|a Suprasanna, Penna \|e verfasserin \|0 (orcid)0000-0002-6572-6190 \|4 aut
773	0	8	\|i Enthalten in \|t Plant physiology and biochemistry \|d Amsterdam [u.a.] : Elsevier Science, 1998 \|g 131, Seite 22-30 \|h Online-Ressource \|w (DE-627)324869193 \|w (DE-600)2031431-0 \|w (DE-576)094080925 \|x 1873-2690 \|7 nnns
773	1	8	\|g volume:131 \|g pages:22-30
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912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
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912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
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912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 42.00 \|j Biologie: Allgemeines
951			\|a AR
952			\|d 131 \|h 22-30

Indexfelder

author_variant	u s k us usk r m s rm rms r l c rl rlc p s ps
matchkey_str	article:18732690:2018----::ocrseadnofagtciiyfeoed
hierarchy_sort_str	2018
bklnumber	42.00
publishDate	2018
allfields	10.1016/j.plaphy.2018.03.027 doi (DE-627)ELV000346675 (ELSEVIER)S0981-9428(18)30150-5 DE-627 ger DE-627 rda eng 630 640 580 DE-600 BIODIV DE-30 fid 42.00 bkl Kadam, Ulhas Sopanrao verfasserin (orcid)0000-0001-8737-167X aut Concerns regarding ‘off-target’ activity of genome editing endonucleases 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement. Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases Shelake, Rahul Mahadev verfasserin (orcid)0000-0003-0691-560X aut Chavhan, Rahul L. verfasserin aut Suprasanna, Penna verfasserin (orcid)0000-0002-6572-6190 aut Enthalten in Plant physiology and biochemistry Amsterdam [u.a.] : Elsevier Science, 1998 131, Seite 22-30 Online-Ressource (DE-627)324869193 (DE-600)2031431-0 (DE-576)094080925 1873-2690 nnns volume:131 pages:22-30 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.00 Biologie: Allgemeines AR 131 22-30
spelling	10.1016/j.plaphy.2018.03.027 doi (DE-627)ELV000346675 (ELSEVIER)S0981-9428(18)30150-5 DE-627 ger DE-627 rda eng 630 640 580 DE-600 BIODIV DE-30 fid 42.00 bkl Kadam, Ulhas Sopanrao verfasserin (orcid)0000-0001-8737-167X aut Concerns regarding ‘off-target’ activity of genome editing endonucleases 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement. Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases Shelake, Rahul Mahadev verfasserin (orcid)0000-0003-0691-560X aut Chavhan, Rahul L. verfasserin aut Suprasanna, Penna verfasserin (orcid)0000-0002-6572-6190 aut Enthalten in Plant physiology and biochemistry Amsterdam [u.a.] : Elsevier Science, 1998 131, Seite 22-30 Online-Ressource (DE-627)324869193 (DE-600)2031431-0 (DE-576)094080925 1873-2690 nnns volume:131 pages:22-30 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.00 Biologie: Allgemeines AR 131 22-30
allfields_unstemmed	10.1016/j.plaphy.2018.03.027 doi (DE-627)ELV000346675 (ELSEVIER)S0981-9428(18)30150-5 DE-627 ger DE-627 rda eng 630 640 580 DE-600 BIODIV DE-30 fid 42.00 bkl Kadam, Ulhas Sopanrao verfasserin (orcid)0000-0001-8737-167X aut Concerns regarding ‘off-target’ activity of genome editing endonucleases 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement. Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases Shelake, Rahul Mahadev verfasserin (orcid)0000-0003-0691-560X aut Chavhan, Rahul L. verfasserin aut Suprasanna, Penna verfasserin (orcid)0000-0002-6572-6190 aut Enthalten in Plant physiology and biochemistry Amsterdam [u.a.] : Elsevier Science, 1998 131, Seite 22-30 Online-Ressource (DE-627)324869193 (DE-600)2031431-0 (DE-576)094080925 1873-2690 nnns volume:131 pages:22-30 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.00 Biologie: Allgemeines AR 131 22-30
allfieldsGer	10.1016/j.plaphy.2018.03.027 doi (DE-627)ELV000346675 (ELSEVIER)S0981-9428(18)30150-5 DE-627 ger DE-627 rda eng 630 640 580 DE-600 BIODIV DE-30 fid 42.00 bkl Kadam, Ulhas Sopanrao verfasserin (orcid)0000-0001-8737-167X aut Concerns regarding ‘off-target’ activity of genome editing endonucleases 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement. Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases Shelake, Rahul Mahadev verfasserin (orcid)0000-0003-0691-560X aut Chavhan, Rahul L. verfasserin aut Suprasanna, Penna verfasserin (orcid)0000-0002-6572-6190 aut Enthalten in Plant physiology and biochemistry Amsterdam [u.a.] : Elsevier Science, 1998 131, Seite 22-30 Online-Ressource (DE-627)324869193 (DE-600)2031431-0 (DE-576)094080925 1873-2690 nnns volume:131 pages:22-30 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.00 Biologie: Allgemeines AR 131 22-30
allfieldsSound	10.1016/j.plaphy.2018.03.027 doi (DE-627)ELV000346675 (ELSEVIER)S0981-9428(18)30150-5 DE-627 ger DE-627 rda eng 630 640 580 DE-600 BIODIV DE-30 fid 42.00 bkl Kadam, Ulhas Sopanrao verfasserin (orcid)0000-0001-8737-167X aut Concerns regarding ‘off-target’ activity of genome editing endonucleases 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement. Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases Shelake, Rahul Mahadev verfasserin (orcid)0000-0003-0691-560X aut Chavhan, Rahul L. verfasserin aut Suprasanna, Penna verfasserin (orcid)0000-0002-6572-6190 aut Enthalten in Plant physiology and biochemistry Amsterdam [u.a.] : Elsevier Science, 1998 131, Seite 22-30 Online-Ressource (DE-627)324869193 (DE-600)2031431-0 (DE-576)094080925 1873-2690 nnns volume:131 pages:22-30 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.00 Biologie: Allgemeines AR 131 22-30
language	English
source	Enthalten in Plant physiology and biochemistry 131, Seite 22-30 volume:131 pages:22-30
sourceStr	Enthalten in Plant physiology and biochemistry 131, Seite 22-30 volume:131 pages:22-30
format_phy_str_mv	Article
bklname	Biologie: Allgemeines
institution	findex.gbv.de
topic_facet	Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases
dewey-raw	630
isfreeaccess_bool	false
container_title	Plant physiology and biochemistry
authorswithroles_txt_mv	Kadam, Ulhas Sopanrao @@aut@@ Shelake, Rahul Mahadev @@aut@@ Chavhan, Rahul L. @@aut@@ Suprasanna, Penna @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	324869193
dewey-sort	3630
id	ELV000346675
language_de	englisch
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author	Kadam, Ulhas Sopanrao
spellingShingle	Kadam, Ulhas Sopanrao ddc 630 fid BIODIV bkl 42.00 misc Genome editing misc Engineered or synthetic nucleases misc Zinc Finger Nucleases (ZFNs) misc Transcription Activator Effector-Like Nucleases (TALENs) misc Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) misc Endonucleases Concerns regarding ‘off-target’ activity of genome editing endonucleases
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topic_title	630 640 580 DE-600 BIODIV DE-30 fid 42.00 bkl Concerns regarding ‘off-target’ activity of genome editing endonucleases Genome editing Engineered or synthetic nucleases Zinc Finger Nucleases (ZFNs) Transcription Activator Effector-Like Nucleases (TALENs) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Endonucleases
topic	ddc 630 fid BIODIV bkl 42.00 misc Genome editing misc Engineered or synthetic nucleases misc Zinc Finger Nucleases (ZFNs) misc Transcription Activator Effector-Like Nucleases (TALENs) misc Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) misc Endonucleases
topic_unstemmed	ddc 630 fid BIODIV bkl 42.00 misc Genome editing misc Engineered or synthetic nucleases misc Zinc Finger Nucleases (ZFNs) misc Transcription Activator Effector-Like Nucleases (TALENs) misc Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) misc Endonucleases
topic_browse	ddc 630 fid BIODIV bkl 42.00 misc Genome editing misc Engineered or synthetic nucleases misc Zinc Finger Nucleases (ZFNs) misc Transcription Activator Effector-Like Nucleases (TALENs) misc Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) misc Endonucleases
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title	Concerns regarding ‘off-target’ activity of genome editing endonucleases
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title_full	Concerns regarding ‘off-target’ activity of genome editing endonucleases
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title_sort	concerns regarding ‘off-target’ activity of genome editing endonucleases
title_auth	Concerns regarding ‘off-target’ activity of genome editing endonucleases
abstract	Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement.
abstractGer	Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement.
abstract_unstemmed	Genome editing (GE) tools ensure targeted mutagenesis and sequence-specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of ‘off-target’ activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the ‘off-target’ activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail ‘off-target’ cleavage. Improvement in target specificity and minimizing ‘off-target’ activity will offer better applications of GE technology in plant biology and crop improvement.
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title_short	Concerns regarding ‘off-target’ activity of genome editing endonucleases
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author2	Shelake, Rahul Mahadev Chavhan, Rahul L. Suprasanna, Penna
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up_date	2024-07-06T17:39:27.715Z
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Concerns regarding ‘off-target’ activity of genome editing endonucleases

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