A microfluidic streaming potential analyzer for label-free DNA detection

In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Li, Yuefang [verfasserIn] Lai, Sze Nga [verfasserIn] Zheng, Bo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization

Übergeordnetes Werk:	Enthalten in: Sensors and actuators / B - Amsterdam [u.a.] : Elsevier Science, 1990, 259, Seite 871-877
Übergeordnetes Werk:	volume:259 ; pages:871-877

DOI / URN:	10.1016/j.snb.2017.12.130

Katalog-ID:	ELV000491632

Internformat


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245	1	0	\|a A microfluidic streaming potential analyzer for label-free DNA detection
264		1	\|c 2017
336			\|a nicht spezifiziert \|b zzz \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection.
650		4	\|a DNA detection
650		4	\|a Streaming potential
650		4	\|a Microfluidics
650		4	\|a Zeta potential
650		4	\|a Peptide nucleic acid
650		4	\|a Hybridization
700	1		\|a Lai, Sze Nga \|e verfasserin \|4 aut
700	1		\|a Zheng, Bo \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Sensors and actuators <Lausanne> / B \|d Amsterdam [u.a.] : Elsevier Science, 1990 \|g 259, Seite 871-877 \|h Online-Ressource \|w (DE-627)306710358 \|w (DE-600)1500731-5 \|w (DE-576)082435855 \|x 0925-4005 \|7 nnns
773	1	8	\|g volume:259 \|g pages:871-877
912			\|a GBV_USEFLAG_U
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912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_187
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2098
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 50.22 \|j Sensorik
936	b	k	\|a 35.07 \|j Chemisches Labor \|j chemische Methoden
951			\|a AR
952			\|d 259 \|h 871-877

Indexfelder

author_variant	y l yl s n l sn snl b z bz
matchkey_str	article:09254005:2017----::mcoliisraigoetaaayefra
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bklnumber	50.22 35.07
publishDate	2017
allfields	10.1016/j.snb.2017.12.130 doi (DE-627)ELV000491632 (ELSEVIER)S0925-4005(17)32463-2 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Li, Yuefang verfasserin aut A microfluidic streaming potential analyzer for label-free DNA detection 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection. DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization Lai, Sze Nga verfasserin aut Zheng, Bo verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 259, Seite 871-877 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:259 pages:871-877 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 259 871-877
spelling	10.1016/j.snb.2017.12.130 doi (DE-627)ELV000491632 (ELSEVIER)S0925-4005(17)32463-2 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Li, Yuefang verfasserin aut A microfluidic streaming potential analyzer for label-free DNA detection 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection. DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization Lai, Sze Nga verfasserin aut Zheng, Bo verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 259, Seite 871-877 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:259 pages:871-877 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 259 871-877
allfields_unstemmed	10.1016/j.snb.2017.12.130 doi (DE-627)ELV000491632 (ELSEVIER)S0925-4005(17)32463-2 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Li, Yuefang verfasserin aut A microfluidic streaming potential analyzer for label-free DNA detection 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection. DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization Lai, Sze Nga verfasserin aut Zheng, Bo verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 259, Seite 871-877 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:259 pages:871-877 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 259 871-877
allfieldsGer	10.1016/j.snb.2017.12.130 doi (DE-627)ELV000491632 (ELSEVIER)S0925-4005(17)32463-2 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Li, Yuefang verfasserin aut A microfluidic streaming potential analyzer for label-free DNA detection 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection. DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization Lai, Sze Nga verfasserin aut Zheng, Bo verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 259, Seite 871-877 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:259 pages:871-877 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 259 871-877
allfieldsSound	10.1016/j.snb.2017.12.130 doi (DE-627)ELV000491632 (ELSEVIER)S0925-4005(17)32463-2 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Li, Yuefang verfasserin aut A microfluidic streaming potential analyzer for label-free DNA detection 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection. DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization Lai, Sze Nga verfasserin aut Zheng, Bo verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 259, Seite 871-877 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:259 pages:871-877 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 259 871-877
language	English
source	Enthalten in Sensors and actuators <Lausanne> / B 259, Seite 871-877 volume:259 pages:871-877
sourceStr	Enthalten in Sensors and actuators <Lausanne> / B 259, Seite 871-877 volume:259 pages:871-877
format_phy_str_mv	Article
bklname	Sensorik Chemisches Labor chemische Methoden
institution	findex.gbv.de
topic_facet	DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization
dewey-raw	530
isfreeaccess_bool	false
container_title	Sensors and actuators <Lausanne> / B
authorswithroles_txt_mv	Li, Yuefang @@aut@@ Lai, Sze Nga @@aut@@ Zheng, Bo @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	306710358
dewey-sort	3530
id	ELV000491632
language_de	englisch
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author	Li, Yuefang
spellingShingle	Li, Yuefang ddc 530 bkl 50.22 bkl 35.07 misc DNA detection misc Streaming potential misc Microfluidics misc Zeta potential misc Peptide nucleic acid misc Hybridization A microfluidic streaming potential analyzer for label-free DNA detection
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topic_title	530 620 DE-600 50.22 bkl 35.07 bkl A microfluidic streaming potential analyzer for label-free DNA detection DNA detection Streaming potential Microfluidics Zeta potential Peptide nucleic acid Hybridization
topic	ddc 530 bkl 50.22 bkl 35.07 misc DNA detection misc Streaming potential misc Microfluidics misc Zeta potential misc Peptide nucleic acid misc Hybridization
topic_unstemmed	ddc 530 bkl 50.22 bkl 35.07 misc DNA detection misc Streaming potential misc Microfluidics misc Zeta potential misc Peptide nucleic acid misc Hybridization
topic_browse	ddc 530 bkl 50.22 bkl 35.07 misc DNA detection misc Streaming potential misc Microfluidics misc Zeta potential misc Peptide nucleic acid misc Hybridization
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title	A microfluidic streaming potential analyzer for label-free DNA detection
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title_full	A microfluidic streaming potential analyzer for label-free DNA detection
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title_sort	a microfluidic streaming potential analyzer for label-free dna detection
title_auth	A microfluidic streaming potential analyzer for label-free DNA detection
abstract	In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection.
abstractGer	In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection.
abstract_unstemmed	In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization of the neutral PNA in the microchannel with the target DNA produced a negatively charged complex, which caused the change of zeta potential and in turn the measured streaming potential. The change of zeta potential was dependent on the hybridization time as well as the concentration and length of the target DNA, through which the complementary target DNA was distinguished from non-complementary DNA with a detection limit of 10 nM. The PNA probes could be recovered in situ and reused without significant capacity loss. The change of the zeta potential was decreased by only 4% after three cycles of PNA-DNA hybridization and PNA recovery. The microfluidic streaming potential analyzer functionalized with PNA probes provides a simple, inexpensive and reusable platform for nucleic acid detection.
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title_short	A microfluidic streaming potential analyzer for label-free DNA detection
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author2	Lai, Sze Nga Zheng, Bo
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up_date	2024-07-06T18:09:15.130Z
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A microfluidic streaming potential analyzer for label-free DNA detection

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