Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification

Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with...
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Gespeichert in:

Autor*in:	Wunderlich, Anja [verfasserIn] Heipieper, Hermann J. [verfasserIn] Elsner, Martin [verfasserIn] Einsiedl, Florian [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation

Übergeordnetes Werk:	Enthalten in: Geochimica et cosmochimica acta - New York, NY [u.a.] : Elsevier, 1950, 239, Seite 275-283
Übergeordnetes Werk:	volume:239 ; pages:275-283

DOI / URN:	10.1016/j.gca.2018.07.037

Katalog-ID:	ELV000539996

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245	1	0	\|a Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification
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520			\|a Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms.
650		4	\|a Denitrification
650		4	\|a Stable isotopes
650		4	\|a Solvent stress
650		4	\|a Phospholipid membrane
650		4	\|a Degree of saturation
700	1		\|a Heipieper, Hermann J. \|e verfasserin \|0 (orcid)0000-0002-3723-9600 \|4 aut
700	1		\|a Elsner, Martin \|e verfasserin \|4 aut
700	1		\|a Einsiedl, Florian \|e verfasserin \|4 aut
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936	b	k	\|a 38.32 \|j Geochemie
936	b	k	\|a 39.29 \|j Theoretische Astronomie: Sonstiges
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Indexfelder

author_variant	a w aw h j h hj hjh m e me f e fe
matchkey_str	article:00167037:2018----::ovnsrsidcdhneimmrnftycdopstoodntiynbceirdctexetfirgntbe
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bklnumber	38.32 39.29
publishDate	2018
allfields	10.1016/j.gca.2018.07.037 doi (DE-627)ELV000539996 (ELSEVIER)S0016-7037(18)30423-X DE-627 ger DE-627 rda eng 550 DE-600 38.32 bkl 39.29 bkl Wunderlich, Anja verfasserin aut Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms. Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation Heipieper, Hermann J. verfasserin (orcid)0000-0002-3723-9600 aut Elsner, Martin verfasserin aut Einsiedl, Florian verfasserin aut Enthalten in Geochimica et cosmochimica acta New York, NY [u.a.] : Elsevier, 1950 239, Seite 275-283 Online-Ressource (DE-627)300898797 (DE-600)1483679-8 (DE-576)120883465 0016-7037 nnns volume:239 pages:275-283 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO SSG-OPC-AST GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 38.32 Geochemie 39.29 Theoretische Astronomie: Sonstiges AR 239 275-283
spelling	10.1016/j.gca.2018.07.037 doi (DE-627)ELV000539996 (ELSEVIER)S0016-7037(18)30423-X DE-627 ger DE-627 rda eng 550 DE-600 38.32 bkl 39.29 bkl Wunderlich, Anja verfasserin aut Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms. Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation Heipieper, Hermann J. verfasserin (orcid)0000-0002-3723-9600 aut Elsner, Martin verfasserin aut Einsiedl, Florian verfasserin aut Enthalten in Geochimica et cosmochimica acta New York, NY [u.a.] : Elsevier, 1950 239, Seite 275-283 Online-Ressource (DE-627)300898797 (DE-600)1483679-8 (DE-576)120883465 0016-7037 nnns volume:239 pages:275-283 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO SSG-OPC-AST GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 38.32 Geochemie 39.29 Theoretische Astronomie: Sonstiges AR 239 275-283
allfields_unstemmed	10.1016/j.gca.2018.07.037 doi (DE-627)ELV000539996 (ELSEVIER)S0016-7037(18)30423-X DE-627 ger DE-627 rda eng 550 DE-600 38.32 bkl 39.29 bkl Wunderlich, Anja verfasserin aut Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms. Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation Heipieper, Hermann J. verfasserin (orcid)0000-0002-3723-9600 aut Elsner, Martin verfasserin aut Einsiedl, Florian verfasserin aut Enthalten in Geochimica et cosmochimica acta New York, NY [u.a.] : Elsevier, 1950 239, Seite 275-283 Online-Ressource (DE-627)300898797 (DE-600)1483679-8 (DE-576)120883465 0016-7037 nnns volume:239 pages:275-283 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO SSG-OPC-AST GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 38.32 Geochemie 39.29 Theoretische Astronomie: Sonstiges AR 239 275-283
allfieldsGer	10.1016/j.gca.2018.07.037 doi (DE-627)ELV000539996 (ELSEVIER)S0016-7037(18)30423-X DE-627 ger DE-627 rda eng 550 DE-600 38.32 bkl 39.29 bkl Wunderlich, Anja verfasserin aut Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms. Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation Heipieper, Hermann J. verfasserin (orcid)0000-0002-3723-9600 aut Elsner, Martin verfasserin aut Einsiedl, Florian verfasserin aut Enthalten in Geochimica et cosmochimica acta New York, NY [u.a.] : Elsevier, 1950 239, Seite 275-283 Online-Ressource (DE-627)300898797 (DE-600)1483679-8 (DE-576)120883465 0016-7037 nnns volume:239 pages:275-283 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO SSG-OPC-AST GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 38.32 Geochemie 39.29 Theoretische Astronomie: Sonstiges AR 239 275-283
allfieldsSound	10.1016/j.gca.2018.07.037 doi (DE-627)ELV000539996 (ELSEVIER)S0016-7037(18)30423-X DE-627 ger DE-627 rda eng 550 DE-600 38.32 bkl 39.29 bkl Wunderlich, Anja verfasserin aut Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms. Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation Heipieper, Hermann J. verfasserin (orcid)0000-0002-3723-9600 aut Elsner, Martin verfasserin aut Einsiedl, Florian verfasserin aut Enthalten in Geochimica et cosmochimica acta New York, NY [u.a.] : Elsevier, 1950 239, Seite 275-283 Online-Ressource (DE-627)300898797 (DE-600)1483679-8 (DE-576)120883465 0016-7037 nnns volume:239 pages:275-283 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO SSG-OPC-AST GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 38.32 Geochemie 39.29 Theoretische Astronomie: Sonstiges AR 239 275-283
language	English
source	Enthalten in Geochimica et cosmochimica acta 239, Seite 275-283 volume:239 pages:275-283
sourceStr	Enthalten in Geochimica et cosmochimica acta 239, Seite 275-283 volume:239 pages:275-283
format_phy_str_mv	Article
bklname	Geochemie Theoretische Astronomie: Sonstiges
institution	findex.gbv.de
topic_facet	Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation
dewey-raw	550
isfreeaccess_bool	false
container_title	Geochimica et cosmochimica acta
authorswithroles_txt_mv	Wunderlich, Anja @@aut@@ Heipieper, Hermann J. @@aut@@ Elsner, Martin @@aut@@ Einsiedl, Florian @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	300898797
dewey-sort	3550
id	ELV000539996
language_de	englisch
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author	Wunderlich, Anja
spellingShingle	Wunderlich, Anja ddc 550 bkl 38.32 bkl 39.29 misc Denitrification misc Stable isotopes misc Solvent stress misc Phospholipid membrane misc Degree of saturation Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification
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topic_title	550 DE-600 38.32 bkl 39.29 bkl Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification Denitrification Stable isotopes Solvent stress Phospholipid membrane Degree of saturation
topic	ddc 550 bkl 38.32 bkl 39.29 misc Denitrification misc Stable isotopes misc Solvent stress misc Phospholipid membrane misc Degree of saturation
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title	Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification
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title_full	Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification
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title_sort	solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification
title_auth	Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification
abstract	Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms.
abstractGer	Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms.
abstract_unstemmed	Microcosm experiments with the well-studied denitrifier Thaurera aromatica show a link between a higher maximum membrane concentration (MMC) of the toxic organic solvents 1-octanol and 4-chlorophenol and a higher degree of saturation (DoS) of the fatty acids in the cell membrane. This coincides with less pronounced stable isotope fractionation during denitrification. We suggest that the change in cell membrane fluidity and the cell’s stress response leads to a decrease in nitrate transport across the cell membrane and/or an increase in the relative ratio of respiratory nitrate reduction rate versus efflux of unreacted nitrate. Both models show that the apparent kinetic isotope effect (AKIE) approach unity and thus reduce the extent of the resulting stable isotope enrichment factor ε15N-NO3 − in dissolved nitrate during denitrification, as experimentally and mathematically shown in this study. This may lead to an underestimation of nitrate reduction determined by nitrate stable isotope analysis in aquatic habitats where various types of stresses may affect the physiology of the driving microorganisms.
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title_short	Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification
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up_date	2024-07-06T18:19:07.530Z
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Solvent stress-induced changes in membrane fatty acid composition of denitrifying bacteria reduce the extent of nitrogen stable isotope fractionation during denitrification

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