Ion chromatographic method for the determination of orotic acid in urine

Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine sam...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Destanoğlu, Orhan [verfasserIn] Zeydanlı, Damla [verfasserIn] Cansever, Mehmet Şerif [verfasserIn] Yılmaz, Gülçin Gümüş [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine

Übergeordnetes Werk:	Enthalten in: Analytical biochemistry - San Diego, Calif. : Elsevier, 1960, 563, Seite 9-14
Übergeordnetes Werk:	volume:563 ; pages:9-14

DOI / URN:	10.1016/j.ab.2018.09.016

Katalog-ID:	ELV001014560

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520			\|a Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories.
650		4	\|a Orotic acid
650		4	\|a Ion chromatography
650		4	\|a Metabolic disorder
650		4	\|a Urea cycle defect
650		4	\|a Argininosuccinic aciduria
650		4	\|a Urine
700	1		\|a Zeydanlı, Damla \|e verfasserin \|4 aut
700	1		\|a Cansever, Mehmet Şerif \|e verfasserin \|4 aut
700	1		\|a Yılmaz, Gülçin Gümüş \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Analytical biochemistry \|d San Diego, Calif. : Elsevier, 1960 \|g 563, Seite 9-14 \|h Online-Ressource \|w (DE-627)25378641X \|w (DE-600)1461105-3 \|w (DE-576)103372997 \|x 1096-0309 \|7 nnns
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936	b	k	\|a 35.71 \|j Biochemische Methoden
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Indexfelder

author_variant	o d od d z dz m ş c mş mşc g g y gg ggy
matchkey_str	article:10960309:2018----::ocrmtgahcehdoteeemntoo
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publishDate	2018
allfields	10.1016/j.ab.2018.09.016 doi (DE-627)ELV001014560 (ELSEVIER)S0003-2697(18)30546-3 DE-627 ger DE-627 rda eng 570 540 DE-600 12 ssgn 35.71 bkl Destanoğlu, Orhan verfasserin aut Ion chromatographic method for the determination of orotic acid in urine 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories. Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine Zeydanlı, Damla verfasserin aut Cansever, Mehmet Şerif verfasserin aut Yılmaz, Gülçin Gümüş verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 563, Seite 9-14 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:563 pages:9-14 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.71 Biochemische Methoden AR 563 9-14
spelling	10.1016/j.ab.2018.09.016 doi (DE-627)ELV001014560 (ELSEVIER)S0003-2697(18)30546-3 DE-627 ger DE-627 rda eng 570 540 DE-600 12 ssgn 35.71 bkl Destanoğlu, Orhan verfasserin aut Ion chromatographic method for the determination of orotic acid in urine 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories. Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine Zeydanlı, Damla verfasserin aut Cansever, Mehmet Şerif verfasserin aut Yılmaz, Gülçin Gümüş verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 563, Seite 9-14 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:563 pages:9-14 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.71 Biochemische Methoden AR 563 9-14
allfields_unstemmed	10.1016/j.ab.2018.09.016 doi (DE-627)ELV001014560 (ELSEVIER)S0003-2697(18)30546-3 DE-627 ger DE-627 rda eng 570 540 DE-600 12 ssgn 35.71 bkl Destanoğlu, Orhan verfasserin aut Ion chromatographic method for the determination of orotic acid in urine 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories. Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine Zeydanlı, Damla verfasserin aut Cansever, Mehmet Şerif verfasserin aut Yılmaz, Gülçin Gümüş verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 563, Seite 9-14 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:563 pages:9-14 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.71 Biochemische Methoden AR 563 9-14
allfieldsGer	10.1016/j.ab.2018.09.016 doi (DE-627)ELV001014560 (ELSEVIER)S0003-2697(18)30546-3 DE-627 ger DE-627 rda eng 570 540 DE-600 12 ssgn 35.71 bkl Destanoğlu, Orhan verfasserin aut Ion chromatographic method for the determination of orotic acid in urine 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories. Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine Zeydanlı, Damla verfasserin aut Cansever, Mehmet Şerif verfasserin aut Yılmaz, Gülçin Gümüş verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 563, Seite 9-14 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:563 pages:9-14 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.71 Biochemische Methoden AR 563 9-14
allfieldsSound	10.1016/j.ab.2018.09.016 doi (DE-627)ELV001014560 (ELSEVIER)S0003-2697(18)30546-3 DE-627 ger DE-627 rda eng 570 540 DE-600 12 ssgn 35.71 bkl Destanoğlu, Orhan verfasserin aut Ion chromatographic method for the determination of orotic acid in urine 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories. Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine Zeydanlı, Damla verfasserin aut Cansever, Mehmet Şerif verfasserin aut Yılmaz, Gülçin Gümüş verfasserin aut Enthalten in Analytical biochemistry San Diego, Calif. : Elsevier, 1960 563, Seite 9-14 Online-Ressource (DE-627)25378641X (DE-600)1461105-3 (DE-576)103372997 1096-0309 nnns volume:563 pages:9-14 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.71 Biochemische Methoden AR 563 9-14
language	English
source	Enthalten in Analytical biochemistry 563, Seite 9-14 volume:563 pages:9-14
sourceStr	Enthalten in Analytical biochemistry 563, Seite 9-14 volume:563 pages:9-14
format_phy_str_mv	Article
bklname	Biochemische Methoden
institution	findex.gbv.de
topic_facet	Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine
dewey-raw	570
isfreeaccess_bool	false
container_title	Analytical biochemistry
authorswithroles_txt_mv	Destanoğlu, Orhan @@aut@@ Zeydanlı, Damla @@aut@@ Cansever, Mehmet Şerif @@aut@@ Yılmaz, Gülçin Gümüş @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	25378641X
dewey-sort	3570
id	ELV001014560
language_de	englisch
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Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. 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author	Destanoğlu, Orhan
spellingShingle	Destanoğlu, Orhan ddc 570 ssgn 12 bkl 35.71 misc Orotic acid misc Ion chromatography misc Metabolic disorder misc Urea cycle defect misc Argininosuccinic aciduria misc Urine Ion chromatographic method for the determination of orotic acid in urine
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topic_title	570 540 DE-600 12 ssgn 35.71 bkl Ion chromatographic method for the determination of orotic acid in urine Orotic acid Ion chromatography Metabolic disorder Urea cycle defect Argininosuccinic aciduria Urine
topic	ddc 570 ssgn 12 bkl 35.71 misc Orotic acid misc Ion chromatography misc Metabolic disorder misc Urea cycle defect misc Argininosuccinic aciduria misc Urine
topic_unstemmed	ddc 570 ssgn 12 bkl 35.71 misc Orotic acid misc Ion chromatography misc Metabolic disorder misc Urea cycle defect misc Argininosuccinic aciduria misc Urine
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title	Ion chromatographic method for the determination of orotic acid in urine
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title_full	Ion chromatographic method for the determination of orotic acid in urine
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author_browse	Destanoğlu, Orhan Zeydanlı, Damla Cansever, Mehmet Şerif Yılmaz, Gülçin Gümüş
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author-letter	Destanoğlu, Orhan
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title_sort	ion chromatographic method for the determination of orotic acid in urine
title_auth	Ion chromatographic method for the determination of orotic acid in urine
abstract	Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories.
abstractGer	Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories.
abstract_unstemmed	Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min−1; the column temperature was 30 °C; sample loop volume was 10 μL. Under optimized conditions, the limit of detection was 0.2 μmol L‾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories.
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title_short	Ion chromatographic method for the determination of orotic acid in urine
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author2	Zeydanlı, Damla Cansever, Mehmet Şerif Yılmaz, Gülçin Gümüş
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up_date	2024-07-06T19:56:15.716Z
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Ion chromatographic method for the determination of orotic acid in urine

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