DNA persistence in soft tissue comparing vodka and absolute ethanol
Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. L...
Ausführliche Beschreibung
Autor*in: |
Baptista, Lais Vicente [verfasserIn] Goodwin, William [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2017 |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: Forensic science international / Genetics Supplement Series - Amsterdam [u.a.] : Elsevier Science, 2008, 6, Seite e46-e48 |
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Übergeordnetes Werk: |
volume:6 ; pages:e46-e48 |
DOI / URN: |
10.1016/j.fsigss.2017.09.008 |
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Katalog-ID: |
ELV001223674 |
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520 | |a Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. | ||
650 | 4 | |a DNA degradation | |
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650 | 4 | |a Drinking alcohol | |
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2017 |
allfields |
10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48 |
spelling |
10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48 |
allfields_unstemmed |
10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48 |
allfieldsGer |
10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48 |
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10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48 |
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title_full |
DNA persistence in soft tissue comparing vodka and absolute ethanol |
author_sort |
Baptista, Lais Vicente |
journal |
Forensic science international / Genetics Supplement Series |
journalStr |
Forensic science international / Genetics Supplement Series |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
300 - Social sciences 600 - Technology |
recordtype |
marc |
publishDateSort |
2017 |
contenttype_str_mv |
zzz |
author_browse |
Baptista, Lais Vicente Goodwin, William |
container_volume |
6 |
class |
340 610 DE-600 44.72 bkl |
format_se |
Elektronische Aufsätze |
author-letter |
Baptista, Lais Vicente |
doi_str_mv |
10.1016/j.fsigss.2017.09.008 |
dewey-full |
340 610 |
author2-role |
verfasserin |
title_sort |
dna persistence in soft tissue comparing vodka and absolute ethanol |
title_auth |
DNA persistence in soft tissue comparing vodka and absolute ethanol |
abstract |
Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. |
abstractGer |
Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. |
abstract_unstemmed |
Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. |
collection_details |
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title_short |
DNA persistence in soft tissue comparing vodka and absolute ethanol |
remote_bool |
true |
author2 |
Goodwin, William |
author2Str |
Goodwin, William |
ppnlink |
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mediatype_str_mv |
c |
isOA_txt |
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hochschulschrift_bool |
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doi_str |
10.1016/j.fsigss.2017.09.008 |
up_date |
2024-07-06T20:38:37.018Z |
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1803863528067563520 |
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