DNA persistence in soft tissue comparing vodka and absolute ethanol

Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. L...
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Gespeichert in:

Autor*in:	Baptista, Lais Vicente [verfasserIn] Goodwin, William [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	DNA degradation DNA preservation Drinking alcohol

Übergeordnetes Werk:	Enthalten in: Forensic science international / Genetics Supplement Series - Amsterdam [u.a.] : Elsevier Science, 2008, 6, Seite e46-e48
Übergeordnetes Werk:	volume:6 ; pages:e46-e48

DOI / URN:	10.1016/j.fsigss.2017.09.008

Katalog-ID:	ELV001223674

Internformat


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245	1	0	\|a DNA persistence in soft tissue comparing vodka and absolute ethanol
264		1	\|c 2017
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337			\|a Computermedien \|b c \|2 rdamedia
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520			\|a Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C.
650		4	\|a DNA degradation
650		4	\|a DNA preservation
650		4	\|a Drinking alcohol
700	1		\|a Goodwin, William \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Forensic science international / Genetics Supplement Series \|d Amsterdam [u.a.] : Elsevier Science, 2008 \|g 6, Seite e46-e48 \|h Online-Ressource \|w (DE-627)582920787 \|w (DE-600)2459032-0 \|w (DE-576)302970630 \|x 1875-1768 \|7 nnns
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912			\|a GBV_USEFLAG_U
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912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_165
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 44.72 \|j Rechtsmedizin
951			\|a AR
952			\|d 6 \|h e46-e48

Indexfelder

author_variant	l v b lv lvb w g wg
matchkey_str	article:18751768:2017----::npritnenotiseoprnvdan
hierarchy_sort_str	2017
bklnumber	44.72
publishDate	2017
allfields	10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48
spelling	10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48
allfields_unstemmed	10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48
allfieldsGer	10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48
allfieldsSound	10.1016/j.fsigss.2017.09.008 doi (DE-627)ELV001223674 (ELSEVIER)S1875-1768(17)30077-X DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Baptista, Lais Vicente verfasserin aut DNA persistence in soft tissue comparing vodka and absolute ethanol 2017 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C. DNA degradation DNA preservation Drinking alcohol Goodwin, William verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 6, Seite e46-e48 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:6 pages:e46-e48 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 6 e46-e48
language	English
source	Enthalten in Forensic science international / Genetics Supplement Series 6, Seite e46-e48 volume:6 pages:e46-e48
sourceStr	Enthalten in Forensic science international / Genetics Supplement Series 6, Seite e46-e48 volume:6 pages:e46-e48
format_phy_str_mv	Article
bklname	Rechtsmedizin
institution	findex.gbv.de
topic_facet	DNA degradation DNA preservation Drinking alcohol
dewey-raw	340
isfreeaccess_bool	false
container_title	Forensic science international / Genetics Supplement Series
authorswithroles_txt_mv	Baptista, Lais Vicente @@aut@@ Goodwin, William @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	582920787
dewey-sort	3340
id	ELV001223674
language_de	englisch
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author	Baptista, Lais Vicente
spellingShingle	Baptista, Lais Vicente ddc 340 bkl 44.72 misc DNA degradation misc DNA preservation misc Drinking alcohol DNA persistence in soft tissue comparing vodka and absolute ethanol
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topic_title	340 610 DE-600 44.72 bkl DNA persistence in soft tissue comparing vodka and absolute ethanol DNA degradation DNA preservation Drinking alcohol
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title	DNA persistence in soft tissue comparing vodka and absolute ethanol
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title_full	DNA persistence in soft tissue comparing vodka and absolute ethanol
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title_sort	dna persistence in soft tissue comparing vodka and absolute ethanol
title_auth	DNA persistence in soft tissue comparing vodka and absolute ethanol
abstract	Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C.
abstractGer	Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C.
abstract_unstemmed	Successful DNA analysis of human remains relies on the collection and preservation of biological material. Low temperatures are typically used to preserve soft tissues; however, this is not always available, for example, after mass disasters and conflict, where the infrastructure has been damaged. Lysis buffers and absolute ethanol have also been shown to be effective at preserving material, but again are not always readily available. This study assess the use of drinking alcohol as an alternative preservative solution for muscle tissue storage. Pig muscle was incubated for up to 42days at different temperatures (−20°C, 25°C, and 37°C) with 95% ethanol, 37.5% ethanol, vodka (37.5%), and no preservative. Samples were collected weekly and analysis was based on agarose gel electrophoresis, DNA quantitation and amplification success of a multiplex with amplicons between 70bp and 384bp. Samples incubated with 37.5% ethanol and vodka had high molecular weight DNA and all samples incubated with preservative solutions generated complete profiles until the last collection point, while samples left untreated had drop-outs after 21days at 25°C and 37°C.
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title_short	DNA persistence in soft tissue comparing vodka and absolute ethanol
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up_date	2024-07-06T20:38:37.018Z
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