Metabolic fingerprinting of carp and rainbow trout seminal plasma

Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their...
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Gespeichert in:

Autor*in:	Dietrich, Mariola A. [verfasserIn] Samczuk, Paulina [verfasserIn] Ciborowski, Michal [verfasserIn] Nynca, Joanna [verfasserIn] Parfieniuk, Ewa [verfasserIn] Pietrowska, Karolina [verfasserIn] Kowalczyk, Tomasz [verfasserIn] Kretowski, Adam [verfasserIn] Ciereszko, Andrzej [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma

Übergeordnetes Werk:	Enthalten in: Aquaculture - Amsterdam [u.a.] : Elsevier Science, 1972, 501, Seite 178-190
Übergeordnetes Werk:	volume:501 ; pages:178-190

DOI / URN:	10.1016/j.aquaculture.2018.11.017

Katalog-ID:	ELV001260766

Internformat


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100	1		\|a Dietrich, Mariola A. \|e verfasserin \|0 (orcid)0000-0002-2525-0941 \|4 aut
245	1	0	\|a Metabolic fingerprinting of carp and rainbow trout seminal plasma
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520			\|a Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species.
650		4	\|a Fish
650		4	\|a Liquid chromatography-mass spectrometry metabolic fingerprinting
650		4	\|a Seminal plasma
700	1		\|a Samczuk, Paulina \|e verfasserin \|0 (orcid)0000-0001-7815-1149 \|4 aut
700	1		\|a Ciborowski, Michal \|e verfasserin \|0 (orcid)0000-0003-3729-0518 \|4 aut
700	1		\|a Nynca, Joanna \|e verfasserin \|4 aut
700	1		\|a Parfieniuk, Ewa \|e verfasserin \|4 aut
700	1		\|a Pietrowska, Karolina \|e verfasserin \|0 (orcid)0000-0002-6639-2823 \|4 aut
700	1		\|a Kowalczyk, Tomasz \|e verfasserin \|4 aut
700	1		\|a Kretowski, Adam \|e verfasserin \|4 aut
700	1		\|a Ciereszko, Andrzej \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Aquaculture \|d Amsterdam [u.a.] : Elsevier Science, 1972 \|g 501, Seite 178-190 \|h Online-Ressource \|w (DE-627)306314002 \|w (DE-600)1495998-7 \|w (DE-576)094503966 \|7 nnns
773	1	8	\|g volume:501 \|g pages:178-190
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936	b	k	\|a 48.68 \|j Aquakultur
951			\|a AR
952			\|d 501 \|h 178-190

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author_variant	m a d ma mad p s ps m c mc j n jn e p ep k p kp t k tk a k ak a c ac
matchkey_str	dietrichmariolaasamczukpaulinaciborowski:2018----:eaoifnepitnocradanoto
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publishDate	2018
allfields	10.1016/j.aquaculture.2018.11.017 doi (DE-627)ELV001260766 (ELSEVIER)S0044-8486(18)31597-7 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin (orcid)0000-0002-2525-0941 aut Metabolic fingerprinting of carp and rainbow trout seminal plasma 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species. Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma Samczuk, Paulina verfasserin (orcid)0000-0001-7815-1149 aut Ciborowski, Michal verfasserin (orcid)0000-0003-3729-0518 aut Nynca, Joanna verfasserin aut Parfieniuk, Ewa verfasserin aut Pietrowska, Karolina verfasserin (orcid)0000-0002-6639-2823 aut Kowalczyk, Tomasz verfasserin aut Kretowski, Adam verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 501, Seite 178-190 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:501 pages:178-190 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 501 178-190
spelling	10.1016/j.aquaculture.2018.11.017 doi (DE-627)ELV001260766 (ELSEVIER)S0044-8486(18)31597-7 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin (orcid)0000-0002-2525-0941 aut Metabolic fingerprinting of carp and rainbow trout seminal plasma 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species. Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma Samczuk, Paulina verfasserin (orcid)0000-0001-7815-1149 aut Ciborowski, Michal verfasserin (orcid)0000-0003-3729-0518 aut Nynca, Joanna verfasserin aut Parfieniuk, Ewa verfasserin aut Pietrowska, Karolina verfasserin (orcid)0000-0002-6639-2823 aut Kowalczyk, Tomasz verfasserin aut Kretowski, Adam verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 501, Seite 178-190 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:501 pages:178-190 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 501 178-190
allfields_unstemmed	10.1016/j.aquaculture.2018.11.017 doi (DE-627)ELV001260766 (ELSEVIER)S0044-8486(18)31597-7 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin (orcid)0000-0002-2525-0941 aut Metabolic fingerprinting of carp and rainbow trout seminal plasma 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species. Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma Samczuk, Paulina verfasserin (orcid)0000-0001-7815-1149 aut Ciborowski, Michal verfasserin (orcid)0000-0003-3729-0518 aut Nynca, Joanna verfasserin aut Parfieniuk, Ewa verfasserin aut Pietrowska, Karolina verfasserin (orcid)0000-0002-6639-2823 aut Kowalczyk, Tomasz verfasserin aut Kretowski, Adam verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 501, Seite 178-190 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:501 pages:178-190 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 501 178-190
allfieldsGer	10.1016/j.aquaculture.2018.11.017 doi (DE-627)ELV001260766 (ELSEVIER)S0044-8486(18)31597-7 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin (orcid)0000-0002-2525-0941 aut Metabolic fingerprinting of carp and rainbow trout seminal plasma 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species. Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma Samczuk, Paulina verfasserin (orcid)0000-0001-7815-1149 aut Ciborowski, Michal verfasserin (orcid)0000-0003-3729-0518 aut Nynca, Joanna verfasserin aut Parfieniuk, Ewa verfasserin aut Pietrowska, Karolina verfasserin (orcid)0000-0002-6639-2823 aut Kowalczyk, Tomasz verfasserin aut Kretowski, Adam verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 501, Seite 178-190 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:501 pages:178-190 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 501 178-190
allfieldsSound	10.1016/j.aquaculture.2018.11.017 doi (DE-627)ELV001260766 (ELSEVIER)S0044-8486(18)31597-7 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin (orcid)0000-0002-2525-0941 aut Metabolic fingerprinting of carp and rainbow trout seminal plasma 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species. Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma Samczuk, Paulina verfasserin (orcid)0000-0001-7815-1149 aut Ciborowski, Michal verfasserin (orcid)0000-0003-3729-0518 aut Nynca, Joanna verfasserin aut Parfieniuk, Ewa verfasserin aut Pietrowska, Karolina verfasserin (orcid)0000-0002-6639-2823 aut Kowalczyk, Tomasz verfasserin aut Kretowski, Adam verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 501, Seite 178-190 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:501 pages:178-190 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 501 178-190
language	English
source	Enthalten in Aquaculture 501, Seite 178-190 volume:501 pages:178-190
sourceStr	Enthalten in Aquaculture 501, Seite 178-190 volume:501 pages:178-190
format_phy_str_mv	Article
bklname	Aquakultur
institution	findex.gbv.de
topic_facet	Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma
dewey-raw	570
isfreeaccess_bool	false
container_title	Aquaculture
authorswithroles_txt_mv	Dietrich, Mariola A. @@aut@@ Samczuk, Paulina @@aut@@ Ciborowski, Michal @@aut@@ Nynca, Joanna @@aut@@ Parfieniuk, Ewa @@aut@@ Pietrowska, Karolina @@aut@@ Kowalczyk, Tomasz @@aut@@ Kretowski, Adam @@aut@@ Ciereszko, Andrzej @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	306314002
dewey-sort	3570
id	ELV001260766
language_de	englisch
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author	Dietrich, Mariola A.
spellingShingle	Dietrich, Mariola A. ddc 570 fid BIODIV bkl 48.68 misc Fish misc Liquid chromatography-mass spectrometry metabolic fingerprinting misc Seminal plasma Metabolic fingerprinting of carp and rainbow trout seminal plasma
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topic_title	570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Metabolic fingerprinting of carp and rainbow trout seminal plasma Fish Liquid chromatography-mass spectrometry metabolic fingerprinting Seminal plasma
topic	ddc 570 fid BIODIV bkl 48.68 misc Fish misc Liquid chromatography-mass spectrometry metabolic fingerprinting misc Seminal plasma
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title	Metabolic fingerprinting of carp and rainbow trout seminal plasma
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title_full	Metabolic fingerprinting of carp and rainbow trout seminal plasma
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author_browse	Dietrich, Mariola A. Samczuk, Paulina Ciborowski, Michal Nynca, Joanna Parfieniuk, Ewa Pietrowska, Karolina Kowalczyk, Tomasz Kretowski, Adam Ciereszko, Andrzej
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title_sort	metabolic fingerprinting of carp and rainbow trout seminal plasma
title_auth	Metabolic fingerprinting of carp and rainbow trout seminal plasma
abstract	Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species.
abstractGer	Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species.
abstract_unstemmed	Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species.
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title_short	Metabolic fingerprinting of carp and rainbow trout seminal plasma
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author2	Samczuk, Paulina Ciborowski, Michal Nynca, Joanna Parfieniuk, Ewa Pietrowska, Karolina Kowalczyk, Tomasz Kretowski, Adam Ciereszko, Andrzej
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up_date	2024-07-06T20:46:22.352Z
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However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. 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Metabolic fingerprinting of carp and rainbow trout seminal plasma

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