Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm (
Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further...
Ausführliche Beschreibung
Autor*in: |
Yue, Shizhong [verfasserIn] Zhang, Huiqi [verfasserIn] Zhen, Huayang [verfasserIn] Lin, Zhiqing [verfasserIn] Qiao, Yuhui [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2018 |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: Microchemical journal - Orlando, Fla. : Academic Press, 1957, 145, Seite 1-8 |
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Übergeordnetes Werk: |
volume:145 ; pages:1-8 |
DOI / URN: |
10.1016/j.microc.2018.10.015 |
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Katalog-ID: |
ELV00143490X |
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520 | |a Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. | ||
650 | 4 | |a Selenium | |
650 | 4 | |a Accumulation | |
650 | 4 | |a Se-enriched earthworm | |
650 | 4 | |a Speciation | |
650 | 4 | |a Bioaccessibility | |
700 | 1 | |a Zhang, Huiqi |e verfasserin |4 aut | |
700 | 1 | |a Zhen, Huayang |e verfasserin |4 aut | |
700 | 1 | |a Lin, Zhiqing |e verfasserin |4 aut | |
700 | 1 | |a Qiao, Yuhui |e verfasserin |4 aut | |
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10.1016/j.microc.2018.10.015 doi (DE-627)ELV00143490X (ELSEVIER)S0026-265X(18)31064-6 DE-627 ger DE-627 rda eng 540 VZ 35.00 bkl Yue, Shizhong verfasserin aut Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. Selenium Accumulation Se-enriched earthworm Speciation Bioaccessibility Zhang, Huiqi verfasserin aut Zhen, Huayang verfasserin aut Lin, Zhiqing verfasserin aut Qiao, Yuhui verfasserin aut Enthalten in Microchemical journal Orlando, Fla. : Academic Press, 1957 145, Seite 1-8 Online-Ressource (DE-627)267840217 (DE-600)1471165-5 (DE-576)259483729 1095-9149 nnns volume:145 pages:1-8 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines VZ AR 145 1-8 |
spelling |
10.1016/j.microc.2018.10.015 doi (DE-627)ELV00143490X (ELSEVIER)S0026-265X(18)31064-6 DE-627 ger DE-627 rda eng 540 VZ 35.00 bkl Yue, Shizhong verfasserin aut Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. Selenium Accumulation Se-enriched earthworm Speciation Bioaccessibility Zhang, Huiqi verfasserin aut Zhen, Huayang verfasserin aut Lin, Zhiqing verfasserin aut Qiao, Yuhui verfasserin aut Enthalten in Microchemical journal Orlando, Fla. : Academic Press, 1957 145, Seite 1-8 Online-Ressource (DE-627)267840217 (DE-600)1471165-5 (DE-576)259483729 1095-9149 nnns volume:145 pages:1-8 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines VZ AR 145 1-8 |
allfields_unstemmed |
10.1016/j.microc.2018.10.015 doi (DE-627)ELV00143490X (ELSEVIER)S0026-265X(18)31064-6 DE-627 ger DE-627 rda eng 540 VZ 35.00 bkl Yue, Shizhong verfasserin aut Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. Selenium Accumulation Se-enriched earthworm Speciation Bioaccessibility Zhang, Huiqi verfasserin aut Zhen, Huayang verfasserin aut Lin, Zhiqing verfasserin aut Qiao, Yuhui verfasserin aut Enthalten in Microchemical journal Orlando, Fla. : Academic Press, 1957 145, Seite 1-8 Online-Ressource (DE-627)267840217 (DE-600)1471165-5 (DE-576)259483729 1095-9149 nnns volume:145 pages:1-8 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines VZ AR 145 1-8 |
allfieldsGer |
10.1016/j.microc.2018.10.015 doi (DE-627)ELV00143490X (ELSEVIER)S0026-265X(18)31064-6 DE-627 ger DE-627 rda eng 540 VZ 35.00 bkl Yue, Shizhong verfasserin aut Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. Selenium Accumulation Se-enriched earthworm Speciation Bioaccessibility Zhang, Huiqi verfasserin aut Zhen, Huayang verfasserin aut Lin, Zhiqing verfasserin aut Qiao, Yuhui verfasserin aut Enthalten in Microchemical journal Orlando, Fla. : Academic Press, 1957 145, Seite 1-8 Online-Ressource (DE-627)267840217 (DE-600)1471165-5 (DE-576)259483729 1095-9149 nnns volume:145 pages:1-8 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines VZ AR 145 1-8 |
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10.1016/j.microc.2018.10.015 doi (DE-627)ELV00143490X (ELSEVIER)S0026-265X(18)31064-6 DE-627 ger DE-627 rda eng 540 VZ 35.00 bkl Yue, Shizhong verfasserin aut Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. Selenium Accumulation Se-enriched earthworm Speciation Bioaccessibility Zhang, Huiqi verfasserin aut Zhen, Huayang verfasserin aut Lin, Zhiqing verfasserin aut Qiao, Yuhui verfasserin aut Enthalten in Microchemical journal Orlando, Fla. : Academic Press, 1957 145, Seite 1-8 Online-Ressource (DE-627)267840217 (DE-600)1471165-5 (DE-576)259483729 1095-9149 nnns volume:145 pages:1-8 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 35.00 Chemie: Allgemeines VZ AR 145 1-8 |
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title |
Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( |
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Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( |
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Yue, Shizhong |
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Microchemical journal |
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Yue, Shizhong Zhang, Huiqi Zhen, Huayang Lin, Zhiqing Qiao, Yuhui |
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540 VZ 35.00 bkl |
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Elektronische Aufsätze |
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Yue, Shizhong |
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10.1016/j.microc.2018.10.015 |
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540 |
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title_sort |
selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( |
title_auth |
Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( |
abstract |
Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. |
abstractGer |
Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. |
abstract_unstemmed |
Selenium (Se) is an essential micronutrient for humans and animals. Earthworm (Eisenia fetida) has been used as animal feed due to its high protein contents and bioactive components. In this laboratory study, earthworm substrates were treated with sodium selenite of 0, 20, or 40 mg kg−1, and further Se accumulation, speciation and bioaccessibility in earthworm tissues were determined. The results showed that in the Se treatments of 20 and 40 mg kg−1 the earthworm biomass and reproduction was not adversely affected, and total Se concentrations in earthworm tissues were 97.78 and 151.56 mg kg−1 dry weight, respectively. Se speciation in E. fetida was extracted with protease XIV, gastric and gastrointestinal enzymes and was analyzed by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Se (IV) was largely biotransformed into organoselenium metabolites, including about 45.8% in SeMet, 29.53% in SeCys2 and 7.3% in unidentified organic Se components. A small fraction of <2.23% was remained in inorganic Se (IV) form. Gastrointestinal digestion was the most efficient way to release Se from earthworm tissues, and Se bioaccessibility based on in vitro simulated gastrointestinal digestion testing was up to 90%. This study suggests that Se-enriched E. fetida could become a good source of Se supplementation in animal feed. |
collection_details |
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title_short |
Selenium accumulation, speciation and bioaccessibility in selenium-enriched earthworm ( |
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Zhang, Huiqi Zhen, Huayang Lin, Zhiqing Qiao, Yuhui |
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up_date |
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