Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells

In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX w...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Lee, Yun Sung [verfasserIn] Kim, Hyun Joo [verfasserIn] Yang, Dae Hyeok [verfasserIn] Chun, Heung Jae [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy

Übergeordnetes Werk:	Enthalten in: Journal of industrial and engineering chemistry - Seoul : KSIEC, 1995, 71, Seite 369-377
Übergeordnetes Werk:	volume:71 ; pages:369-377

DOI / URN:	10.1016/j.jiec.2018.11.048

Katalog-ID:	ELV001494023

Internformat


LEADER	01000caa a22002652 4500
001	ELV001494023
003	DE-627
005	20230524122049.0
007	cr uuu---uuuuu
008	230428s2018 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1016/j.jiec.2018.11.048 \|2 doi
035			\|a (DE-627)ELV001494023
035			\|a (ELSEVIER)S1226-086X(18)31351-0
040			\|a DE-627 \|b ger \|c DE-627 \|e rda
041			\|a eng
082	0	4	\|a 600 \|a 540 \|q DE-600
100	1		\|a Lee, Yun Sung \|e verfasserin \|4 aut
245	1	0	\|a Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells
264		1	\|c 2018
336			\|a nicht spezifiziert \|b zzz \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy.
650		4	\|a Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL)
650		4	\|a Paclitaxel (PTX)
650		4	\|a MPEG-PCL-PTX
650		4	\|a 4T1 cell line
650		4	\|a Cancer therapy
700	1		\|a Kim, Hyun Joo \|e verfasserin \|4 aut
700	1		\|a Yang, Dae Hyeok \|e verfasserin \|4 aut
700	1		\|a Chun, Heung Jae \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Journal of industrial and engineering chemistry \|d Seoul : KSIEC, 1995 \|g 71, Seite 369-377 \|w (DE-627)391337238 \|w (DE-600)2152565-1 \|w (DE-576)28474784X \|x 1226-086X \|7 nnns
773	1	8	\|g volume:71 \|g pages:369-377
912			\|a GBV_USEFLAG_U
912			\|a SYSFLAG_U
912			\|a GBV_ELV
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
951			\|a AR
952			\|d 71 \|h 369-377

Indexfelder

author_variant	y s l ys ysl h j k hj hjk d h y dh dhy h j c hj hjc
matchkey_str	article:1226086X:2018----::rprtoadniacrciiyvlainfefsebepciaecnua
hierarchy_sort_str	2018
publishDate	2018
allfields	10.1016/j.jiec.2018.11.048 doi (DE-627)ELV001494023 (ELSEVIER)S1226-086X(18)31351-0 DE-627 ger DE-627 rda eng 600 540 DE-600 Lee, Yun Sung verfasserin aut Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy. Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy Kim, Hyun Joo verfasserin aut Yang, Dae Hyeok verfasserin aut Chun, Heung Jae verfasserin aut Enthalten in Journal of industrial and engineering chemistry Seoul : KSIEC, 1995 71, Seite 369-377 (DE-627)391337238 (DE-600)2152565-1 (DE-576)28474784X 1226-086X nnns volume:71 pages:369-377 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 71 369-377
spelling	10.1016/j.jiec.2018.11.048 doi (DE-627)ELV001494023 (ELSEVIER)S1226-086X(18)31351-0 DE-627 ger DE-627 rda eng 600 540 DE-600 Lee, Yun Sung verfasserin aut Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy. Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy Kim, Hyun Joo verfasserin aut Yang, Dae Hyeok verfasserin aut Chun, Heung Jae verfasserin aut Enthalten in Journal of industrial and engineering chemistry Seoul : KSIEC, 1995 71, Seite 369-377 (DE-627)391337238 (DE-600)2152565-1 (DE-576)28474784X 1226-086X nnns volume:71 pages:369-377 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 71 369-377
allfields_unstemmed	10.1016/j.jiec.2018.11.048 doi (DE-627)ELV001494023 (ELSEVIER)S1226-086X(18)31351-0 DE-627 ger DE-627 rda eng 600 540 DE-600 Lee, Yun Sung verfasserin aut Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy. Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy Kim, Hyun Joo verfasserin aut Yang, Dae Hyeok verfasserin aut Chun, Heung Jae verfasserin aut Enthalten in Journal of industrial and engineering chemistry Seoul : KSIEC, 1995 71, Seite 369-377 (DE-627)391337238 (DE-600)2152565-1 (DE-576)28474784X 1226-086X nnns volume:71 pages:369-377 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 71 369-377
allfieldsGer	10.1016/j.jiec.2018.11.048 doi (DE-627)ELV001494023 (ELSEVIER)S1226-086X(18)31351-0 DE-627 ger DE-627 rda eng 600 540 DE-600 Lee, Yun Sung verfasserin aut Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy. Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy Kim, Hyun Joo verfasserin aut Yang, Dae Hyeok verfasserin aut Chun, Heung Jae verfasserin aut Enthalten in Journal of industrial and engineering chemistry Seoul : KSIEC, 1995 71, Seite 369-377 (DE-627)391337238 (DE-600)2152565-1 (DE-576)28474784X 1226-086X nnns volume:71 pages:369-377 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 71 369-377
allfieldsSound	10.1016/j.jiec.2018.11.048 doi (DE-627)ELV001494023 (ELSEVIER)S1226-086X(18)31351-0 DE-627 ger DE-627 rda eng 600 540 DE-600 Lee, Yun Sung verfasserin aut Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy. Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy Kim, Hyun Joo verfasserin aut Yang, Dae Hyeok verfasserin aut Chun, Heung Jae verfasserin aut Enthalten in Journal of industrial and engineering chemistry Seoul : KSIEC, 1995 71, Seite 369-377 (DE-627)391337238 (DE-600)2152565-1 (DE-576)28474784X 1226-086X nnns volume:71 pages:369-377 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 71 369-377
language	English
source	Enthalten in Journal of industrial and engineering chemistry 71, Seite 369-377 volume:71 pages:369-377
sourceStr	Enthalten in Journal of industrial and engineering chemistry 71, Seite 369-377 volume:71 pages:369-377
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy
dewey-raw	600
isfreeaccess_bool	false
container_title	Journal of industrial and engineering chemistry
authorswithroles_txt_mv	Lee, Yun Sung @@aut@@ Kim, Hyun Joo @@aut@@ Yang, Dae Hyeok @@aut@@ Chun, Heung Jae @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	391337238
dewey-sort	3600
id	ELV001494023
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV001494023</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230524122049.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230428s2018 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.jiec.2018.11.048</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV001494023</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S1226-086X(18)31351-0</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">600</subfield><subfield code="a">540</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Lee, Yun Sung</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2018</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Paclitaxel (PTX)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">MPEG-PCL-PTX</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">4T1 cell line</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cancer therapy</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kim, Hyun Joo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Dae Hyeok</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chun, Heung Jae</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of industrial and engineering chemistry</subfield><subfield code="d">Seoul : KSIEC, 1995</subfield><subfield code="g">71, Seite 369-377</subfield><subfield code="w">(DE-627)391337238</subfield><subfield code="w">(DE-600)2152565-1</subfield><subfield code="w">(DE-576)28474784X</subfield><subfield code="x">1226-086X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:71</subfield><subfield code="g">pages:369-377</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">71</subfield><subfield code="h">369-377</subfield></datafield></record></collection>
author	Lee, Yun Sung
spellingShingle	Lee, Yun Sung ddc 600 misc Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) misc Paclitaxel (PTX) misc MPEG-PCL-PTX misc 4T1 cell line misc Cancer therapy Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells
authorStr	Lee, Yun Sung
ppnlink_with_tag_str_mv	@@773@@(DE-627)391337238
format	electronic Article
dewey-ones	600 - Technology 540 - Chemistry & allied sciences
delete_txt_mv	keep
author_role	aut aut aut aut
collection	elsevier
remote_str	true
illustrated	Not Illustrated
issn	1226-086X
topic_title	600 540 DE-600 Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) Paclitaxel (PTX) MPEG-PCL-PTX 4T1 cell line Cancer therapy
topic	ddc 600 misc Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) misc Paclitaxel (PTX) misc MPEG-PCL-PTX misc 4T1 cell line misc Cancer therapy
topic_unstemmed	ddc 600 misc Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) misc Paclitaxel (PTX) misc MPEG-PCL-PTX misc 4T1 cell line misc Cancer therapy
topic_browse	ddc 600 misc Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) misc Paclitaxel (PTX) misc MPEG-PCL-PTX misc 4T1 cell line misc Cancer therapy
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Journal of industrial and engineering chemistry
hierarchy_parent_id	391337238
dewey-tens	600 - Technology 540 - Chemistry
hierarchy_top_title	Journal of industrial and engineering chemistry
isfreeaccess_txt	false
familylinks_str_mv	(DE-627)391337238 (DE-600)2152565-1 (DE-576)28474784X
title	Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells
ctrlnum	(DE-627)ELV001494023 (ELSEVIER)S1226-086X(18)31351-0
title_full	Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells
author_sort	Lee, Yun Sung
journal	Journal of industrial and engineering chemistry
journalStr	Journal of industrial and engineering chemistry
lang_code	eng
isOA_bool	false
dewey-hundreds	600 - Technology 500 - Science
recordtype	marc
publishDateSort	2018
contenttype_str_mv	zzz
container_start_page	369
author_browse	Lee, Yun Sung Kim, Hyun Joo Yang, Dae Hyeok Chun, Heung Jae
container_volume	71
class	600 540 DE-600
format_se	Elektronische Aufsätze
author-letter	Lee, Yun Sung
doi_str_mv	10.1016/j.jiec.2018.11.048
dewey-full	600 540
author2-role	verfasserin
title_sort	preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated mpeg-pcl micelles on 4t1 cells
title_auth	Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells
abstract	In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy.
abstractGer	In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy.
abstract_unstemmed	In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy.
collection_details	GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393
title_short	Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells
remote_bool	true
author2	Kim, Hyun Joo Yang, Dae Hyeok Chun, Heung Jae
author2Str	Kim, Hyun Joo Yang, Dae Hyeok Chun, Heung Jae
ppnlink	391337238
mediatype_str_mv	c
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1016/j.jiec.2018.11.048
up_date	2024-07-06T21:33:42.610Z
_version_	1803866994232000512
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV001494023</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230524122049.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230428s2018 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.jiec.2018.11.048</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV001494023</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S1226-086X(18)31351-0</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">600</subfield><subfield code="a">540</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Lee, Yun Sung</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2018</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">In this study, we prepared self-assembled micelles with methoxy poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL) for paclitaxel (PTX) delivery. MPEG-PCL was synthesized by ring-opening polymerization in which the hydroxyl group was substituted for a carboxyl group by succinic anhydride. PTX was then coupled to carboxylated MPEG-PCL through esterification (MPEG-PCL-PTX). MPEG-PCL-PTX micelles were prepared by a solvent evaporation method. Conjugation of PTX to MPEG-PCL-PTX was confirmed by 1H NMR. MPEG-PCL-PTX micelles self-assembled into micellar nanoparticles at concentrations above 2.5×10−2 mg/mL, as evaluated by fluorescence spectrometry. DLS and TEM analyses revealed that the micelles were globular in shape with an average size of 111nm. The drug release studies under three pH conditions demonstrated that PTX release from MPEG-PCL-PTX micelles was entirely dependent upon the hydrolysis of ester bond between PTX and MPEG-PCL copolymer, therefore, no severe initial burst release but pH sensitive profile was observed. In vitro tests with the 4T1 cell line showed that conjugation of PTX to MPEG-PCL led to improved cellular uptake and apoptosis compared to free PTX, supporting the feasibility of using the micelles as drug carriers for cancer therapy.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Poly(ethylene glycol)-b-poly(ε-caprolactone) (MPEG-PCL)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Paclitaxel (PTX)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">MPEG-PCL-PTX</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">4T1 cell line</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cancer therapy</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kim, Hyun Joo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Dae Hyeok</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chun, Heung Jae</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of industrial and engineering chemistry</subfield><subfield code="d">Seoul : KSIEC, 1995</subfield><subfield code="g">71, Seite 369-377</subfield><subfield code="w">(DE-627)391337238</subfield><subfield code="w">(DE-600)2152565-1</subfield><subfield code="w">(DE-576)28474784X</subfield><subfield code="x">1226-086X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:71</subfield><subfield code="g">pages:369-377</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">71</subfield><subfield code="h">369-377</subfield></datafield></record></collection>
score	7.399349

Nicht das Richtige dabei?

Schreiben Sie uns!

Preparation and anticancer activity evaluation of self-assembled paclitaxel conjugated MPEG-PCL micelles on 4T1 cells

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?