Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida

Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET...
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Autor*in:	Shieh, Hester F [verfasserIn] Tracy, Sarah A [verfasserIn] Hong, Charles R [verfasserIn] Chalphin, Alexander V [verfasserIn] Ahmed, Azra [verfasserIn] Rohrer, Lucas [verfasserIn] Zurakowski, David [verfasserIn] Fauza, Dario O [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele

Übergeordnetes Werk:	Enthalten in: Journal of pediatric surgery - Orlando, Fla. : Elsevier, 1966, 54
Übergeordnetes Werk:	volume:54

DOI / URN:	10.1016/j.jpedsurg.2018.10.086

Katalog-ID:	ELV001714112

Internformat


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024	7		\|a 10.1016/j.jpedsurg.2018.10.086 \|2 doi
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035			\|a (ELSEVIER)S0022-3468(18)30746-2
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100	1		\|a Shieh, Hester F \|e verfasserin \|4 aut
245	1	0	\|a Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida
264		1	\|c 2018
336			\|a nicht spezifiziert \|b zzz \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study).
650		4	\|a Amniotic mesenchymal stem cells
650		4	\|a Fetal stem cells
650		4	\|a Fetal cell therapy
650		4	\|a Fetal therapy
650		4	\|a Transamniotic stem cell therapy
650		4	\|a TRASCET
650		4	\|a Spina bifida
650		4	\|a Myelomeningocele
700	1		\|a Tracy, Sarah A \|e verfasserin \|4 aut
700	1		\|a Hong, Charles R \|e verfasserin \|4 aut
700	1		\|a Chalphin, Alexander V \|e verfasserin \|4 aut
700	1		\|a Ahmed, Azra \|e verfasserin \|4 aut
700	1		\|a Rohrer, Lucas \|e verfasserin \|4 aut
700	1		\|a Zurakowski, David \|e verfasserin \|4 aut
700	1		\|a Fauza, Dario O \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Journal of pediatric surgery \|d Orlando, Fla. : Elsevier, 1966 \|g 54 \|h Online-Ressource \|w (DE-627)325699542 \|w (DE-600)2039299-0 \|w (DE-576)094422338 \|x 1531-5037 \|7 nnns
773	1	8	\|g volume:54
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912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
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912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
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912			\|a GBV_ILN_4125
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912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 44.65 \|j Chirurgie
951			\|a AR
952			\|d 54

Indexfelder

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matchkey_str	article:15315037:2018----::rnanoiseclteayrseiaabt
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publishDate	2018
allfields	10.1016/j.jpedsurg.2018.10.086 doi (DE-627)ELV001714112 (ELSEVIER)S0022-3468(18)30746-2 DE-627 ger DE-627 rda eng 610 DE-600 44.65 bkl Shieh, Hester F verfasserin aut Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study). Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele Tracy, Sarah A verfasserin aut Hong, Charles R verfasserin aut Chalphin, Alexander V verfasserin aut Ahmed, Azra verfasserin aut Rohrer, Lucas verfasserin aut Zurakowski, David verfasserin aut Fauza, Dario O verfasserin aut Enthalten in Journal of pediatric surgery Orlando, Fla. : Elsevier, 1966 54 Online-Ressource (DE-627)325699542 (DE-600)2039299-0 (DE-576)094422338 1531-5037 nnns volume:54 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.65 Chirurgie AR 54
spelling	10.1016/j.jpedsurg.2018.10.086 doi (DE-627)ELV001714112 (ELSEVIER)S0022-3468(18)30746-2 DE-627 ger DE-627 rda eng 610 DE-600 44.65 bkl Shieh, Hester F verfasserin aut Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study). Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele Tracy, Sarah A verfasserin aut Hong, Charles R verfasserin aut Chalphin, Alexander V verfasserin aut Ahmed, Azra verfasserin aut Rohrer, Lucas verfasserin aut Zurakowski, David verfasserin aut Fauza, Dario O verfasserin aut Enthalten in Journal of pediatric surgery Orlando, Fla. : Elsevier, 1966 54 Online-Ressource (DE-627)325699542 (DE-600)2039299-0 (DE-576)094422338 1531-5037 nnns volume:54 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.65 Chirurgie AR 54
allfields_unstemmed	10.1016/j.jpedsurg.2018.10.086 doi (DE-627)ELV001714112 (ELSEVIER)S0022-3468(18)30746-2 DE-627 ger DE-627 rda eng 610 DE-600 44.65 bkl Shieh, Hester F verfasserin aut Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study). Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele Tracy, Sarah A verfasserin aut Hong, Charles R verfasserin aut Chalphin, Alexander V verfasserin aut Ahmed, Azra verfasserin aut Rohrer, Lucas verfasserin aut Zurakowski, David verfasserin aut Fauza, Dario O verfasserin aut Enthalten in Journal of pediatric surgery Orlando, Fla. : Elsevier, 1966 54 Online-Ressource (DE-627)325699542 (DE-600)2039299-0 (DE-576)094422338 1531-5037 nnns volume:54 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.65 Chirurgie AR 54
allfieldsGer	10.1016/j.jpedsurg.2018.10.086 doi (DE-627)ELV001714112 (ELSEVIER)S0022-3468(18)30746-2 DE-627 ger DE-627 rda eng 610 DE-600 44.65 bkl Shieh, Hester F verfasserin aut Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study). Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele Tracy, Sarah A verfasserin aut Hong, Charles R verfasserin aut Chalphin, Alexander V verfasserin aut Ahmed, Azra verfasserin aut Rohrer, Lucas verfasserin aut Zurakowski, David verfasserin aut Fauza, Dario O verfasserin aut Enthalten in Journal of pediatric surgery Orlando, Fla. : Elsevier, 1966 54 Online-Ressource (DE-627)325699542 (DE-600)2039299-0 (DE-576)094422338 1531-5037 nnns volume:54 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.65 Chirurgie AR 54
allfieldsSound	10.1016/j.jpedsurg.2018.10.086 doi (DE-627)ELV001714112 (ELSEVIER)S0022-3468(18)30746-2 DE-627 ger DE-627 rda eng 610 DE-600 44.65 bkl Shieh, Hester F verfasserin aut Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida 2018 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study). Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele Tracy, Sarah A verfasserin aut Hong, Charles R verfasserin aut Chalphin, Alexander V verfasserin aut Ahmed, Azra verfasserin aut Rohrer, Lucas verfasserin aut Zurakowski, David verfasserin aut Fauza, Dario O verfasserin aut Enthalten in Journal of pediatric surgery Orlando, Fla. : Elsevier, 1966 54 Online-Ressource (DE-627)325699542 (DE-600)2039299-0 (DE-576)094422338 1531-5037 nnns volume:54 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.65 Chirurgie AR 54
language	English
source	Enthalten in Journal of pediatric surgery 54 volume:54
sourceStr	Enthalten in Journal of pediatric surgery 54 volume:54
format_phy_str_mv	Article
bklname	Chirurgie
institution	findex.gbv.de
topic_facet	Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele
dewey-raw	610
isfreeaccess_bool	false
container_title	Journal of pediatric surgery
authorswithroles_txt_mv	Shieh, Hester F @@aut@@ Tracy, Sarah A @@aut@@ Hong, Charles R @@aut@@ Chalphin, Alexander V @@aut@@ Ahmed, Azra @@aut@@ Rohrer, Lucas @@aut@@ Zurakowski, David @@aut@@ Fauza, Dario O @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	325699542
dewey-sort	3610
id	ELV001714112
language_de	englisch
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Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. 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author	Shieh, Hester F
spellingShingle	Shieh, Hester F ddc 610 bkl 44.65 misc Amniotic mesenchymal stem cells misc Fetal stem cells misc Fetal cell therapy misc Fetal therapy misc Transamniotic stem cell therapy misc TRASCET misc Spina bifida misc Myelomeningocele Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida
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topic_title	610 DE-600 44.65 bkl Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida Amniotic mesenchymal stem cells Fetal stem cells Fetal cell therapy Fetal therapy Transamniotic stem cell therapy TRASCET Spina bifida Myelomeningocele
topic	ddc 610 bkl 44.65 misc Amniotic mesenchymal stem cells misc Fetal stem cells misc Fetal cell therapy misc Fetal therapy misc Transamniotic stem cell therapy misc TRASCET misc Spina bifida misc Myelomeningocele
topic_unstemmed	ddc 610 bkl 44.65 misc Amniotic mesenchymal stem cells misc Fetal stem cells misc Fetal cell therapy misc Fetal therapy misc Transamniotic stem cell therapy misc TRASCET misc Spina bifida misc Myelomeningocele
topic_browse	ddc 610 bkl 44.65 misc Amniotic mesenchymal stem cells misc Fetal stem cells misc Fetal cell therapy misc Fetal therapy misc Transamniotic stem cell therapy misc TRASCET misc Spina bifida misc Myelomeningocele
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title	Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida
ctrlnum	(DE-627)ELV001714112 (ELSEVIER)S0022-3468(18)30746-2
title_full	Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida
author_sort	Shieh, Hester F
journal	Journal of pediatric surgery
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author_browse	Shieh, Hester F Tracy, Sarah A Hong, Charles R Chalphin, Alexander V Ahmed, Azra Rohrer, Lucas Zurakowski, David Fauza, Dario O
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author-letter	Shieh, Hester F
doi_str_mv	10.1016/j.jpedsurg.2018.10.086
dewey-full	610
author2-role	verfasserin
title_sort	transamniotic stem cell therapy (trascet) in a rabbit model of spina bifida
title_auth	Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida
abstract	Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study).
abstractGer	Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study).
abstract_unstemmed	Purpose: Transamniotic stem cell therapy (TRASCET) with select mesenchymal stem cells (MSCs) has been shown to induce partial or complete skin coverage of spina bifida in rodents. Clinical translation of this emerging therapy hinges on its efficacy in larger animal models. We sought to study TRASCET in a model requiring intra-amniotic injections 60 times larger than those performed in the rat.Methods: Rabbit fetuses (n = 65) with surgically created spina bifida were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intra-amniotic injections of either saline or a concentrated suspension of amniotic fluid MSCs (afMSCs) at the time of operation. Infused afMSCs consisted of banked heterologous rabbit afMSCs with mesenchymal identity confirmed by flow cytometry, labeled with green fluorescent protein. Defect coverage at term was blindly categorized only if the presence of a distinctive neoskin was confirmed histologically. Statistical comparisons were by logistic regression and the likelihood ratio test.Results: Among survivors with spina bifida (n = 19), there were statistically significant higher rates of defect coverage (all partial) in the afMSC group when compared with the saline and untreated groups (0–50%; p = 0.022–0.036), with no difference between the saline and untreated groups (p = 1.00). Donor afMSCs were identified locally, though sparsely and not in the neoskin.Conclusions: Concentrated intra-amniotic injection of amniotic mesenchymal stem cells can induce partial coverage of experimental spina bifida in a leporine model. Transamniotic stem cell therapy may become a feasible strategy in the prenatal management of spina bifida.Level of Evidence: N/A (animal and laboratory study).
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title_short	Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida
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author2	Tracy, Sarah A Hong, Charles R Chalphin, Alexander V Ahmed, Azra Rohrer, Lucas Zurakowski, David Fauza, Dario O
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doi_str	10.1016/j.jpedsurg.2018.10.086
up_date	2024-07-06T22:19:21.504Z
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Transamniotic stem cell therapy (TRASCET) in a rabbit model of spina bifida

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