Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge

The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specif...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Lotti, Tommaso [verfasserIn] Carretti, Emiliano [verfasserIn] Berti, Debora [verfasserIn] Martina, Maria Raffaella [verfasserIn] Lubello, Claudio [verfasserIn] Malpei, Francesca [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy

Übergeordnetes Werk:	Enthalten in: Journal of environmental management - Amsterdam [u.a.] : Elsevier, 1990, 236, Seite 649-656
Übergeordnetes Werk:	volume:236 ; pages:649-656

DOI / URN:	10.1016/j.jenvman.2019.01.054

Katalog-ID:	ELV001750739

Internformat


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520			\|a The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context.
650		4	\|a Extraction/recovery
650		4	\|a Anammox biofilm
650		4	\|a EPS
650		4	\|a Extracellular polymeric substances
650		4	\|a Excess sludge
650		4	\|a Biorefinery
650		4	\|a Circular economy
700	1		\|a Carretti, Emiliano \|e verfasserin \|0 (orcid)0000-0001-5140-3123 \|4 aut
700	1		\|a Berti, Debora \|e verfasserin \|0 (orcid)0000-0001-8967-560X \|4 aut
700	1		\|a Martina, Maria Raffaella \|e verfasserin \|0 (orcid)0000-0003-2489-8639 \|4 aut
700	1		\|a Lubello, Claudio \|e verfasserin \|0 (orcid)0000-0002-1423-1884 \|4 aut
700	1		\|a Malpei, Francesca \|e verfasserin \|0 (orcid)0000-0003-1479-377X \|4 aut
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936	b	k	\|a 48.00 \|j Land- und Forstwirtschaft: Allgemeines
951			\|a AR
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publishDate	2019
allfields	10.1016/j.jenvman.2019.01.054 doi (DE-627)ELV001750739 (ELSEVIER)S0301-4797(19)30055-6 DE-627 ger DE-627 rda eng 333.7 690 DE-600 48.00 bkl Lotti, Tommaso verfasserin (orcid)0000-0003-0379-4822 aut Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context. Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy Carretti, Emiliano verfasserin (orcid)0000-0001-5140-3123 aut Berti, Debora verfasserin (orcid)0000-0001-8967-560X aut Martina, Maria Raffaella verfasserin (orcid)0000-0003-2489-8639 aut Lubello, Claudio verfasserin (orcid)0000-0002-1423-1884 aut Malpei, Francesca verfasserin (orcid)0000-0003-1479-377X aut Enthalten in Journal of environmental management Amsterdam [u.a.] : Elsevier, 1990 236, Seite 649-656 Online-Ressource (DE-627)266892868 (DE-600)1469206-5 (DE-576)10434461X 1095-8630 nnns volume:236 pages:649-656 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-FOR GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.00 Land- und Forstwirtschaft: Allgemeines AR 236 649-656
spelling	10.1016/j.jenvman.2019.01.054 doi (DE-627)ELV001750739 (ELSEVIER)S0301-4797(19)30055-6 DE-627 ger DE-627 rda eng 333.7 690 DE-600 48.00 bkl Lotti, Tommaso verfasserin (orcid)0000-0003-0379-4822 aut Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context. Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy Carretti, Emiliano verfasserin (orcid)0000-0001-5140-3123 aut Berti, Debora verfasserin (orcid)0000-0001-8967-560X aut Martina, Maria Raffaella verfasserin (orcid)0000-0003-2489-8639 aut Lubello, Claudio verfasserin (orcid)0000-0002-1423-1884 aut Malpei, Francesca verfasserin (orcid)0000-0003-1479-377X aut Enthalten in Journal of environmental management Amsterdam [u.a.] : Elsevier, 1990 236, Seite 649-656 Online-Ressource (DE-627)266892868 (DE-600)1469206-5 (DE-576)10434461X 1095-8630 nnns volume:236 pages:649-656 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-FOR GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.00 Land- und Forstwirtschaft: Allgemeines AR 236 649-656
allfields_unstemmed	10.1016/j.jenvman.2019.01.054 doi (DE-627)ELV001750739 (ELSEVIER)S0301-4797(19)30055-6 DE-627 ger DE-627 rda eng 333.7 690 DE-600 48.00 bkl Lotti, Tommaso verfasserin (orcid)0000-0003-0379-4822 aut Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context. Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy Carretti, Emiliano verfasserin (orcid)0000-0001-5140-3123 aut Berti, Debora verfasserin (orcid)0000-0001-8967-560X aut Martina, Maria Raffaella verfasserin (orcid)0000-0003-2489-8639 aut Lubello, Claudio verfasserin (orcid)0000-0002-1423-1884 aut Malpei, Francesca verfasserin (orcid)0000-0003-1479-377X aut Enthalten in Journal of environmental management Amsterdam [u.a.] : Elsevier, 1990 236, Seite 649-656 Online-Ressource (DE-627)266892868 (DE-600)1469206-5 (DE-576)10434461X 1095-8630 nnns volume:236 pages:649-656 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-FOR GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.00 Land- und Forstwirtschaft: Allgemeines AR 236 649-656
allfieldsGer	10.1016/j.jenvman.2019.01.054 doi (DE-627)ELV001750739 (ELSEVIER)S0301-4797(19)30055-6 DE-627 ger DE-627 rda eng 333.7 690 DE-600 48.00 bkl Lotti, Tommaso verfasserin (orcid)0000-0003-0379-4822 aut Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context. Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy Carretti, Emiliano verfasserin (orcid)0000-0001-5140-3123 aut Berti, Debora verfasserin (orcid)0000-0001-8967-560X aut Martina, Maria Raffaella verfasserin (orcid)0000-0003-2489-8639 aut Lubello, Claudio verfasserin (orcid)0000-0002-1423-1884 aut Malpei, Francesca verfasserin (orcid)0000-0003-1479-377X aut Enthalten in Journal of environmental management Amsterdam [u.a.] : Elsevier, 1990 236, Seite 649-656 Online-Ressource (DE-627)266892868 (DE-600)1469206-5 (DE-576)10434461X 1095-8630 nnns volume:236 pages:649-656 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-FOR GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.00 Land- und Forstwirtschaft: Allgemeines AR 236 649-656
allfieldsSound	10.1016/j.jenvman.2019.01.054 doi (DE-627)ELV001750739 (ELSEVIER)S0301-4797(19)30055-6 DE-627 ger DE-627 rda eng 333.7 690 DE-600 48.00 bkl Lotti, Tommaso verfasserin (orcid)0000-0003-0379-4822 aut Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context. Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy Carretti, Emiliano verfasserin (orcid)0000-0001-5140-3123 aut Berti, Debora verfasserin (orcid)0000-0001-8967-560X aut Martina, Maria Raffaella verfasserin (orcid)0000-0003-2489-8639 aut Lubello, Claudio verfasserin (orcid)0000-0002-1423-1884 aut Malpei, Francesca verfasserin (orcid)0000-0003-1479-377X aut Enthalten in Journal of environmental management Amsterdam [u.a.] : Elsevier, 1990 236, Seite 649-656 Online-Ressource (DE-627)266892868 (DE-600)1469206-5 (DE-576)10434461X 1095-8630 nnns volume:236 pages:649-656 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-FOR GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.00 Land- und Forstwirtschaft: Allgemeines AR 236 649-656
language	English
source	Enthalten in Journal of environmental management 236, Seite 649-656 volume:236 pages:649-656
sourceStr	Enthalten in Journal of environmental management 236, Seite 649-656 volume:236 pages:649-656
format_phy_str_mv	Article
bklname	Land- und Forstwirtschaft: Allgemeines
institution	findex.gbv.de
topic_facet	Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy
dewey-raw	333.7
isfreeaccess_bool	false
container_title	Journal of environmental management
authorswithroles_txt_mv	Lotti, Tommaso @@aut@@ Carretti, Emiliano @@aut@@ Berti, Debora @@aut@@ Martina, Maria Raffaella @@aut@@ Lubello, Claudio @@aut@@ Malpei, Francesca @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	266892868
dewey-sort	3333.7
id	ELV001750739
language_de	englisch
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author	Lotti, Tommaso
spellingShingle	Lotti, Tommaso ddc 333.7 bkl 48.00 misc Extraction/recovery misc Anammox biofilm misc EPS misc Extracellular polymeric substances misc Excess sludge misc Biorefinery misc Circular economy Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge
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topic_title	333.7 690 DE-600 48.00 bkl Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge Extraction/recovery Anammox biofilm EPS Extracellular polymeric substances Excess sludge Biorefinery Circular economy
topic	ddc 333.7 bkl 48.00 misc Extraction/recovery misc Anammox biofilm misc EPS misc Extracellular polymeric substances misc Excess sludge misc Biorefinery misc Circular economy
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title	Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge
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title_full	Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge
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title_sort	extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge
title_auth	Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge
abstract	The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context.
abstractGer	The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context.
abstract_unstemmed	The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 ± 0.04 and 0.21 ± 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-β motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context.
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title_short	Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge
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author2	Carretti, Emiliano Berti, Debora Martina, Maria Raffaella Lubello, Claudio Malpei, Francesca
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up_date	2024-07-06T22:26:46.342Z
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Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge

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