Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device

Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Chen, Guoqiang [verfasserIn] Gerrior, Alisha [verfasserIn] Hale, Geoffrey [verfasserIn] Ghosh, Raja [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants

Übergeordnetes Werk:	Enthalten in: Biochemical engineering journal - Amsterdam [u.a.] : Elsevier, 1998, 147, Seite 48-56
Übergeordnetes Werk:	volume:147 ; pages:48-56

DOI / URN:	10.1016/j.bej.2019.04.003

Katalog-ID:	ELV00249731X

Internformat


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245	1	0	\|a Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device
264		1	\|c 2019
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520			\|a Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices.
650		4	\|a Cuboid packed-bed
650		4	\|a Chromatography
650		4	\|a Preparative column
650		4	\|a Efficiency
650		4	\|a Resolution
650		4	\|a Monoclonal antibody charge variants
700	1		\|a Gerrior, Alisha \|e verfasserin \|4 aut
700	1		\|a Hale, Geoffrey \|e verfasserin \|4 aut
700	1		\|a Ghosh, Raja \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Biochemical engineering journal \|d Amsterdam [u.a.] : Elsevier, 1998 \|g 147, Seite 48-56 \|h Online-Ressource \|w (DE-627)320500349 \|w (DE-600)2012139-8 \|w (DE-576)098330160 \|7 nnns
773	1	8	\|g volume:147 \|g pages:48-56
912			\|a GBV_USEFLAG_U
912			\|a SYSFLAG_U
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912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 58.30 \|j Biotechnologie
936	b	k	\|a 58.00 \|j Chemische Technik: Allgemeines
951			\|a AR
952			\|d 147 \|h 48-56

Indexfelder

author_variant	g c gc a g ag g h gh r g rg
matchkey_str	chenguoqianggerrioralishahalegeoffreygho:2019----:esbltsuyfhfatoainfoolnlnioyhreains
hierarchy_sort_str	2019
bklnumber	58.30 58.00
publishDate	2019
allfields	10.1016/j.bej.2019.04.003 doi (DE-627)ELV00249731X (ELSEVIER)S1369-703X(19)30115-9 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Chen, Guoqiang verfasserin aut Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices. Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants Gerrior, Alisha verfasserin aut Hale, Geoffrey verfasserin aut Ghosh, Raja verfasserin aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 147, Seite 48-56 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:147 pages:48-56 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 147 48-56
spelling	10.1016/j.bej.2019.04.003 doi (DE-627)ELV00249731X (ELSEVIER)S1369-703X(19)30115-9 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Chen, Guoqiang verfasserin aut Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices. Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants Gerrior, Alisha verfasserin aut Hale, Geoffrey verfasserin aut Ghosh, Raja verfasserin aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 147, Seite 48-56 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:147 pages:48-56 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 147 48-56
allfields_unstemmed	10.1016/j.bej.2019.04.003 doi (DE-627)ELV00249731X (ELSEVIER)S1369-703X(19)30115-9 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Chen, Guoqiang verfasserin aut Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices. Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants Gerrior, Alisha verfasserin aut Hale, Geoffrey verfasserin aut Ghosh, Raja verfasserin aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 147, Seite 48-56 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:147 pages:48-56 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 147 48-56
allfieldsGer	10.1016/j.bej.2019.04.003 doi (DE-627)ELV00249731X (ELSEVIER)S1369-703X(19)30115-9 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Chen, Guoqiang verfasserin aut Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices. Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants Gerrior, Alisha verfasserin aut Hale, Geoffrey verfasserin aut Ghosh, Raja verfasserin aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 147, Seite 48-56 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:147 pages:48-56 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 147 48-56
allfieldsSound	10.1016/j.bej.2019.04.003 doi (DE-627)ELV00249731X (ELSEVIER)S1369-703X(19)30115-9 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Chen, Guoqiang verfasserin aut Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices. Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants Gerrior, Alisha verfasserin aut Hale, Geoffrey verfasserin aut Ghosh, Raja verfasserin aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 147, Seite 48-56 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:147 pages:48-56 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 147 48-56
language	English
source	Enthalten in Biochemical engineering journal 147, Seite 48-56 volume:147 pages:48-56
sourceStr	Enthalten in Biochemical engineering journal 147, Seite 48-56 volume:147 pages:48-56
format_phy_str_mv	Article
bklname	Biotechnologie Chemische Technik: Allgemeines
institution	findex.gbv.de
topic_facet	Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants
dewey-raw	660
isfreeaccess_bool	false
container_title	Biochemical engineering journal
authorswithroles_txt_mv	Chen, Guoqiang @@aut@@ Gerrior, Alisha @@aut@@ Hale, Geoffrey @@aut@@ Ghosh, Raja @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	320500349
dewey-sort	3660
id	ELV00249731X
language_de	englisch
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author	Chen, Guoqiang
spellingShingle	Chen, Guoqiang ddc 660 bkl 58.30 bkl 58.00 misc Cuboid packed-bed misc Chromatography misc Preparative column misc Efficiency misc Resolution misc Monoclonal antibody charge variants Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device
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topic_title	660 540 DE-600 58.30 bkl 58.00 bkl Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device Cuboid packed-bed Chromatography Preparative column Efficiency Resolution Monoclonal antibody charge variants
topic	ddc 660 bkl 58.30 bkl 58.00 misc Cuboid packed-bed misc Chromatography misc Preparative column misc Efficiency misc Resolution misc Monoclonal antibody charge variants
topic_unstemmed	ddc 660 bkl 58.30 bkl 58.00 misc Cuboid packed-bed misc Chromatography misc Preparative column misc Efficiency misc Resolution misc Monoclonal antibody charge variants
topic_browse	ddc 660 bkl 58.30 bkl 58.00 misc Cuboid packed-bed misc Chromatography misc Preparative column misc Efficiency misc Resolution misc Monoclonal antibody charge variants
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title	Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device
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title_full	Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device
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author_browse	Chen, Guoqiang Gerrior, Alisha Hale, Geoffrey Ghosh, Raja
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title_sort	feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device
title_auth	Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device
abstract	Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices.
abstractGer	Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices.
abstract_unstemmed	Cuboid packed-bed devices have been proposed as efficient alternative to preparative columns. In the current study, we discuss a challenging purification application i.e. separation of monoclonal antibody (mAb) charge variants by ion-exchange chromatography based on subtle charge heterogeneity. Head-to-head experiments were carried out using a cuboid packed-bed device and its equivalent column. Efficiency related metrics such as plate height and peak attributes such as asymmetry and tailing were first compared. Charge variants containing mAb samples were then fractionated. The results obtained showed that the cuboid packed-bed device outperformed the column, both in terms of efficiency and resolution. For instance, at 30 cm/h superficial velocity, the cuboid packed-bed device had 14240 theoretical plates/m as opposed to 4409 theoretical plates/m in the column. Also, at 15 cm/h superficial velocity and 150 mL linear gradient, the resolution between the main peak and the basic peaks were 1.29 and 1.12 respectively for the cuboid packed-bed device and the column. The enhancement in resolution was particularly significant at lower flow rates and when shorter gradients were used. For instance, a 100 mL gradient could be used with the cuboid packed-bed device to achieve resolution comparable to that obtained with the column using 150 mL gradient. Simulations showed that high purity (>94%) and recovery (>95%) of the main mAb peak component could simultaneously be achieved using the cuboid packed-bed device. In this paper we also discuss a new in-situ resin packing method for cuboid packed-bed devices.
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title_short	Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device
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up_date	2024-07-07T00:55:27.502Z
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Feasibility study of the fractionation of monoclonal antibody charge variants using a cuboid packed-bed device

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