Visualizing primordial germ cell migration in embryos of rice field eel (

In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containi...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Xiao, Qing [verfasserIn] Sun, Yiqing Liang, Xiao Zhang, Lihan Onxayvieng, Kommaly Li, Zhong Li, Dapeng

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Primordial germ cells 3′ untranslated region

Systematik:	WA 15000

Übergeordnetes Werk:	Enthalten in: Comparative biochemistry and physiology - London : Elsevier, 2011, 235, Seite 62-69
Übergeordnetes Werk:	volume:235 ; pages:62-69

DOI / URN:	10.1016/j.cbpb.2019.06.002

Katalog-ID:	ELV002558718

Internformat


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245	1	0	\|a Visualizing primordial germ cell migration in embryos of rice field eel (
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520			\|a In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel.
650		4	\|a Primordial germ cells
650		4	\|a 3′ untranslated region
700	1		\|a Sun, Yiqing \|4 oth
700	1		\|a Liang, Xiao \|4 oth
700	1		\|a Zhang, Lihan \|4 oth
700	1		\|a Onxayvieng, Kommaly \|4 oth
700	1		\|a Li, Zhong \|4 oth
700	1		\|a Li, Dapeng \|4 oth
773	0	8	\|i Enthalten in \|t Comparative biochemistry and physiology \|d London : Elsevier, 2011 \|g 235, Seite 62-69 \|w (DE-627)300185448 \|w (DE-600)1481604-0 \|x 187-91107 \|7 nnns
773	1	8	\|g volume:235 \|g pages:62-69
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Indexfelder

author_variant	q x qx
matchkey_str	article:18791107:2019----::iulznpiodagrclmgainnmr
hierarchy_sort_str	2019
bklnumber	35.70
publishDate	2019
allfields	10.1016/j.cbpb.2019.06.002 doi (DE-627)ELV002558718 (ELSEVIER)S1096-4959(19)30037-5 DE-627 ger DE-627 rda eng 590 540 DE-600 12 ssgn BIODIV DE-30 fid WA 15000 rvk 35.70 bkl Xiao, Qing verfasserin aut Visualizing primordial germ cell migration in embryos of rice field eel ( 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel. Primordial germ cells 3′ untranslated region Sun, Yiqing oth Liang, Xiao oth Zhang, Lihan oth Onxayvieng, Kommaly oth Li, Zhong oth Li, Dapeng oth Enthalten in Comparative biochemistry and physiology London : Elsevier, 2011 235, Seite 62-69 (DE-627)300185448 (DE-600)1481604-0 187-91107 nnns volume:235 pages:62-69 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-CHE SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 WA 15000 35.70 AR 235 62-69
spelling	10.1016/j.cbpb.2019.06.002 doi (DE-627)ELV002558718 (ELSEVIER)S1096-4959(19)30037-5 DE-627 ger DE-627 rda eng 590 540 DE-600 12 ssgn BIODIV DE-30 fid WA 15000 rvk 35.70 bkl Xiao, Qing verfasserin aut Visualizing primordial germ cell migration in embryos of rice field eel ( 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel. Primordial germ cells 3′ untranslated region Sun, Yiqing oth Liang, Xiao oth Zhang, Lihan oth Onxayvieng, Kommaly oth Li, Zhong oth Li, Dapeng oth Enthalten in Comparative biochemistry and physiology London : Elsevier, 2011 235, Seite 62-69 (DE-627)300185448 (DE-600)1481604-0 187-91107 nnns volume:235 pages:62-69 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-CHE SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 WA 15000 35.70 AR 235 62-69
allfields_unstemmed	10.1016/j.cbpb.2019.06.002 doi (DE-627)ELV002558718 (ELSEVIER)S1096-4959(19)30037-5 DE-627 ger DE-627 rda eng 590 540 DE-600 12 ssgn BIODIV DE-30 fid WA 15000 rvk 35.70 bkl Xiao, Qing verfasserin aut Visualizing primordial germ cell migration in embryos of rice field eel ( 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel. Primordial germ cells 3′ untranslated region Sun, Yiqing oth Liang, Xiao oth Zhang, Lihan oth Onxayvieng, Kommaly oth Li, Zhong oth Li, Dapeng oth Enthalten in Comparative biochemistry and physiology London : Elsevier, 2011 235, Seite 62-69 (DE-627)300185448 (DE-600)1481604-0 187-91107 nnns volume:235 pages:62-69 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-CHE SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 WA 15000 35.70 AR 235 62-69
allfieldsGer	10.1016/j.cbpb.2019.06.002 doi (DE-627)ELV002558718 (ELSEVIER)S1096-4959(19)30037-5 DE-627 ger DE-627 rda eng 590 540 DE-600 12 ssgn BIODIV DE-30 fid WA 15000 rvk 35.70 bkl Xiao, Qing verfasserin aut Visualizing primordial germ cell migration in embryos of rice field eel ( 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel. Primordial germ cells 3′ untranslated region Sun, Yiqing oth Liang, Xiao oth Zhang, Lihan oth Onxayvieng, Kommaly oth Li, Zhong oth Li, Dapeng oth Enthalten in Comparative biochemistry and physiology London : Elsevier, 2011 235, Seite 62-69 (DE-627)300185448 (DE-600)1481604-0 187-91107 nnns volume:235 pages:62-69 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-CHE SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 WA 15000 35.70 AR 235 62-69
allfieldsSound	10.1016/j.cbpb.2019.06.002 doi (DE-627)ELV002558718 (ELSEVIER)S1096-4959(19)30037-5 DE-627 ger DE-627 rda eng 590 540 DE-600 12 ssgn BIODIV DE-30 fid WA 15000 rvk 35.70 bkl Xiao, Qing verfasserin aut Visualizing primordial germ cell migration in embryos of rice field eel ( 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel. Primordial germ cells 3′ untranslated region Sun, Yiqing oth Liang, Xiao oth Zhang, Lihan oth Onxayvieng, Kommaly oth Li, Zhong oth Li, Dapeng oth Enthalten in Comparative biochemistry and physiology London : Elsevier, 2011 235, Seite 62-69 (DE-627)300185448 (DE-600)1481604-0 187-91107 nnns volume:235 pages:62-69 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-CHE SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 WA 15000 35.70 AR 235 62-69
language	English
source	Enthalten in Comparative biochemistry and physiology 235, Seite 62-69 volume:235 pages:62-69
sourceStr	Enthalten in Comparative biochemistry and physiology 235, Seite 62-69 volume:235 pages:62-69
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Primordial germ cells 3′ untranslated region
dewey-raw	590
isfreeaccess_bool	false
container_title	Comparative biochemistry and physiology
authorswithroles_txt_mv	Xiao, Qing @@aut@@ Sun, Yiqing @@oth@@ Liang, Xiao @@oth@@ Zhang, Lihan @@oth@@ Onxayvieng, Kommaly @@oth@@ Li, Zhong @@oth@@ Li, Dapeng @@oth@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	300185448
dewey-sort	3590
id	ELV002558718
language_de	englisch
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author	Xiao, Qing
spellingShingle	Xiao, Qing ddc 590 ssgn 12 fid BIODIV rvk WA 15000 bkl 35.70 misc Primordial germ cells misc 3′ untranslated region Visualizing primordial germ cell migration in embryos of rice field eel (
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topic_title	590 540 DE-600 12 ssgn BIODIV DE-30 fid WA 15000 rvk 35.70 bkl Visualizing primordial germ cell migration in embryos of rice field eel ( Primordial germ cells 3′ untranslated region
topic	ddc 590 ssgn 12 fid BIODIV rvk WA 15000 bkl 35.70 misc Primordial germ cells misc 3′ untranslated region
topic_unstemmed	ddc 590 ssgn 12 fid BIODIV rvk WA 15000 bkl 35.70 misc Primordial germ cells misc 3′ untranslated region
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title	Visualizing primordial germ cell migration in embryos of rice field eel (
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title_full	Visualizing primordial germ cell migration in embryos of rice field eel (
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doi_str_mv	10.1016/j.cbpb.2019.06.002
dewey-full	590 540
title_sort	visualizing primordial germ cell migration in embryos of rice field eel (
title_auth	Visualizing primordial germ cell migration in embryos of rice field eel (
abstract	In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel.
abstractGer	In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel.
abstract_unstemmed	In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3′ untranslated region (3′UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3′UTRs labeled PGCs over a wider time frame than those containing vasa 3′UTR, suggesting that nanos3 and dnd 3′UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel.
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title_short	Visualizing primordial germ cell migration in embryos of rice field eel (
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author2	Sun, Yiqing Liang, Xiao Zhang, Lihan Onxayvieng, Kommaly Li, Zhong Li, Dapeng
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up_date	2024-07-06T16:40:30.804Z
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Visualizing primordial germ cell migration in embryos of rice field eel (

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