Subarachnoid hemorrhage in rats – Visualizing blood distribution
Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Met...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Simader, Elisabeth [verfasserIn] Budinsky, Lubos [verfasserIn] Helbich, Thomas H. [verfasserIn] Sherif, Camillo [verfasserIn] Höftberger, Romana [verfasserIn] Kasprian, Gregor [verfasserIn] Raunegger, Thomas [verfasserIn] Hacker, Philipp [verfasserIn] Ankersmit, Hendrik Jan [verfasserIn] Beer, Lucian [verfasserIn] Haider, Thomas [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of neuroscience methods - Amsterdam [u.a.] : Elsevier Science, 1979, 325 |
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| Übergeordnetes Werk: |
volume:325 |
| DOI / URN: |
10.1016/j.jneumeth.2019.108370 |
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ELV00266335X |
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| 520 | |a Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. | ||
| 650 | 4 | |a Subarachnoid haemorrhage (SAH) | |
| 650 | 4 | |a Preclinical model of SAH | |
| 650 | 4 | |a Gadolinium | |
| 650 | 4 | |a Preclinical MRI | |
| 700 | 1 | |a Budinsky, Lubos |e verfasserin |4 aut | |
| 700 | 1 | |a Helbich, Thomas H. |e verfasserin |4 aut | |
| 700 | 1 | |a Sherif, Camillo |e verfasserin |4 aut | |
| 700 | 1 | |a Höftberger, Romana |e verfasserin |4 aut | |
| 700 | 1 | |a Kasprian, Gregor |e verfasserin |4 aut | |
| 700 | 1 | |a Raunegger, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Hacker, Philipp |e verfasserin |4 aut | |
| 700 | 1 | |a Ankersmit, Hendrik Jan |e verfasserin |4 aut | |
| 700 | 1 | |a Beer, Lucian |e verfasserin |0 (orcid)0000-0003-4388-7580 |4 aut | |
| 700 | 1 | |a Haider, Thomas |e verfasserin |0 (orcid)0000-0003-3192-8453 |4 aut | |
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10.1016/j.jneumeth.2019.108370 doi (DE-627)ELV00266335X (ELSEVIER)S0165-0270(19)30227-4 DE-627 ger DE-627 rda eng 610 DE-600 44.90 bkl Simader, Elisabeth verfasserin aut Subarachnoid hemorrhage in rats – Visualizing blood distribution 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. Subarachnoid haemorrhage (SAH) Preclinical model of SAH Gadolinium Preclinical MRI Budinsky, Lubos verfasserin aut Helbich, Thomas H. verfasserin aut Sherif, Camillo verfasserin aut Höftberger, Romana verfasserin aut Kasprian, Gregor verfasserin aut Raunegger, Thomas verfasserin aut Hacker, Philipp verfasserin aut Ankersmit, Hendrik Jan verfasserin aut Beer, Lucian verfasserin (orcid)0000-0003-4388-7580 aut Haider, Thomas verfasserin (orcid)0000-0003-3192-8453 aut Enthalten in Journal of neuroscience methods Amsterdam [u.a.] : Elsevier Science, 1979 325 Online-Ressource (DE-627)306659786 (DE-600)1500499-5 (DE-576)081986416 1872-678X nnns volume:325 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2336 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4338 44.90 Neurologie AR 325 |
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10.1016/j.jneumeth.2019.108370 doi (DE-627)ELV00266335X (ELSEVIER)S0165-0270(19)30227-4 DE-627 ger DE-627 rda eng 610 DE-600 44.90 bkl Simader, Elisabeth verfasserin aut Subarachnoid hemorrhage in rats – Visualizing blood distribution 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. Subarachnoid haemorrhage (SAH) Preclinical model of SAH Gadolinium Preclinical MRI Budinsky, Lubos verfasserin aut Helbich, Thomas H. verfasserin aut Sherif, Camillo verfasserin aut Höftberger, Romana verfasserin aut Kasprian, Gregor verfasserin aut Raunegger, Thomas verfasserin aut Hacker, Philipp verfasserin aut Ankersmit, Hendrik Jan verfasserin aut Beer, Lucian verfasserin (orcid)0000-0003-4388-7580 aut Haider, Thomas verfasserin (orcid)0000-0003-3192-8453 aut Enthalten in Journal of neuroscience methods Amsterdam [u.a.] : Elsevier Science, 1979 325 Online-Ressource (DE-627)306659786 (DE-600)1500499-5 (DE-576)081986416 1872-678X nnns volume:325 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2336 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4338 44.90 Neurologie AR 325 |
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10.1016/j.jneumeth.2019.108370 doi (DE-627)ELV00266335X (ELSEVIER)S0165-0270(19)30227-4 DE-627 ger DE-627 rda eng 610 DE-600 44.90 bkl Simader, Elisabeth verfasserin aut Subarachnoid hemorrhage in rats – Visualizing blood distribution 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. Subarachnoid haemorrhage (SAH) Preclinical model of SAH Gadolinium Preclinical MRI Budinsky, Lubos verfasserin aut Helbich, Thomas H. verfasserin aut Sherif, Camillo verfasserin aut Höftberger, Romana verfasserin aut Kasprian, Gregor verfasserin aut Raunegger, Thomas verfasserin aut Hacker, Philipp verfasserin aut Ankersmit, Hendrik Jan verfasserin aut Beer, Lucian verfasserin (orcid)0000-0003-4388-7580 aut Haider, Thomas verfasserin (orcid)0000-0003-3192-8453 aut Enthalten in Journal of neuroscience methods Amsterdam [u.a.] : Elsevier Science, 1979 325 Online-Ressource (DE-627)306659786 (DE-600)1500499-5 (DE-576)081986416 1872-678X nnns volume:325 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2336 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4338 44.90 Neurologie AR 325 |
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10.1016/j.jneumeth.2019.108370 doi (DE-627)ELV00266335X (ELSEVIER)S0165-0270(19)30227-4 DE-627 ger DE-627 rda eng 610 DE-600 44.90 bkl Simader, Elisabeth verfasserin aut Subarachnoid hemorrhage in rats – Visualizing blood distribution 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. Subarachnoid haemorrhage (SAH) Preclinical model of SAH Gadolinium Preclinical MRI Budinsky, Lubos verfasserin aut Helbich, Thomas H. verfasserin aut Sherif, Camillo verfasserin aut Höftberger, Romana verfasserin aut Kasprian, Gregor verfasserin aut Raunegger, Thomas verfasserin aut Hacker, Philipp verfasserin aut Ankersmit, Hendrik Jan verfasserin aut Beer, Lucian verfasserin (orcid)0000-0003-4388-7580 aut Haider, Thomas verfasserin (orcid)0000-0003-3192-8453 aut Enthalten in Journal of neuroscience methods Amsterdam [u.a.] : Elsevier Science, 1979 325 Online-Ressource (DE-627)306659786 (DE-600)1500499-5 (DE-576)081986416 1872-678X nnns volume:325 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2336 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4338 44.90 Neurologie AR 325 |
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10.1016/j.jneumeth.2019.108370 doi (DE-627)ELV00266335X (ELSEVIER)S0165-0270(19)30227-4 DE-627 ger DE-627 rda eng 610 DE-600 44.90 bkl Simader, Elisabeth verfasserin aut Subarachnoid hemorrhage in rats – Visualizing blood distribution 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. Subarachnoid haemorrhage (SAH) Preclinical model of SAH Gadolinium Preclinical MRI Budinsky, Lubos verfasserin aut Helbich, Thomas H. verfasserin aut Sherif, Camillo verfasserin aut Höftberger, Romana verfasserin aut Kasprian, Gregor verfasserin aut Raunegger, Thomas verfasserin aut Hacker, Philipp verfasserin aut Ankersmit, Hendrik Jan verfasserin aut Beer, Lucian verfasserin (orcid)0000-0003-4388-7580 aut Haider, Thomas verfasserin (orcid)0000-0003-3192-8453 aut Enthalten in Journal of neuroscience methods Amsterdam [u.a.] : Elsevier Science, 1979 325 Online-Ressource (DE-627)306659786 (DE-600)1500499-5 (DE-576)081986416 1872-678X nnns volume:325 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2336 GBV_ILN_4037 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4326 GBV_ILN_4334 GBV_ILN_4338 44.90 Neurologie AR 325 |
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Simader, Elisabeth @@aut@@ Budinsky, Lubos @@aut@@ Helbich, Thomas H. @@aut@@ Sherif, Camillo @@aut@@ Höftberger, Romana @@aut@@ Kasprian, Gregor @@aut@@ Raunegger, Thomas @@aut@@ Hacker, Philipp @@aut@@ Ankersmit, Hendrik Jan @@aut@@ Beer, Lucian @@aut@@ Haider, Thomas @@aut@@ |
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2019-01-01T00:00:00Z |
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englisch |
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Simader, Elisabeth |
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subarachnoid hemorrhage in rats – visualizing blood distribution |
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Subarachnoid hemorrhage in rats – Visualizing blood distribution |
| abstract |
Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. |
| abstractGer |
Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. |
| abstract_unstemmed |
Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV00266335X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230524153914.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230429s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.jneumeth.2019.108370</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV00266335X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0165-0270(19)30227-4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.90</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Simader, Elisabeth</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Subarachnoid hemorrhage in rats – Visualizing blood distribution</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background: The aims of this study were to assess the feasibility of magnetic resonance imaging (MRI) to track the in vivo distribution of autologous, injected blood in a subarachnoid hemorrhage model (SAH), and to evaluate whether this technique results in observable morphological detriment.New Method: We used an SAH model of stereotactic injection of autologous blood into the prechiasmatic cistern in Sprague Dawley rats. To visualize its in vivo distribution, a gadolinium-containing contrast agent was added to the autologous blood prior to injection. MRI was performed on a 9.4 T Bruker Biospec scanner preoperatively, as well as at variable time points between 30 min to 23 days after SAH. T1-weighted and diffusion-weighted images were acquired. The morphological examination was completed by a histopathological work-up.Results: Upon injection of contrast agent-enriched autologous blood, enhancement was observed in the entire subarachnoid space within 30 min of injection. Total clearance was noted at the first postoperative day. SAH induction did not result in changes in clinical scores or on histopathological or radiological images.Comparison with Existing Methods: We modified an established method to allow in vivo MRI monitoring of subarachnoid blood distribution in an SAH model.Conclusion: This technique could be used to evaluate the distribution of blood components during the development of novel SAH models. Since no additional morphological detriment was observed, this technique could be used as a validation tool to verify correct application and induction in preclinical SAH models.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Subarachnoid haemorrhage (SAH)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Preclinical model of SAH</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gadolinium</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Preclinical MRI</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Budinsky, Lubos</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Helbich, Thomas H.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield 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