Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus
Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Li, Lingxia [verfasserIn] Wu, Jinyan [verfasserIn] Cao, Xiao'’an [verfasserIn] Zhou, Jianhua [verfasserIn] Yin, Shuanghui [verfasserIn] Yang, Shunli [verfasserIn] Feng, Qian [verfasserIn] Du, Ping [verfasserIn] Liu, Yongjie [verfasserIn] Shang, Youjun [verfasserIn] Liu, Yongsheng [verfasserIn] Liu, Xiangtao [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
Peste des petits ruminants virus |
|---|
| Übergeordnetes Werk: |
Enthalten in: Research in veterinary science - Amsterdam [u.a.] : Elsevier, 1960, 125, Seite 195-204 |
|---|---|
| Übergeordnetes Werk: |
volume:125 ; pages:195-204 |
| DOI / URN: |
10.1016/j.rvsc.2019.06.011 |
|---|
| Katalog-ID: |
ELV002758474 |
|---|
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| 245 | 1 | 0 | |a Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus |
| 264 | 1 | |c 2019 | |
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| 520 | |a Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. | ||
| 650 | 4 | |a Peste des petits ruminants virus | |
| 650 | 4 | |a Bone marrow derived dendritic cells | |
| 650 | 4 | |a iTRAQ | |
| 650 | 4 | |a Proteomics | |
| 700 | 1 | |a Wu, Jinyan |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Xiao'’an |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Jianhua |e verfasserin |4 aut | |
| 700 | 1 | |a Yin, Shuanghui |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Shunli |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Du, Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yongjie |e verfasserin |4 aut | |
| 700 | 1 | |a Shang, Youjun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yongsheng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiangtao |e verfasserin |4 aut | |
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10.1016/j.rvsc.2019.06.011 doi (DE-627)ELV002758474 (ELSEVIER)S0034-5288(19)30010-4 DE-627 ger DE-627 rda eng 630 DE-600 22 ssgn Li, Lingxia verfasserin aut Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. Peste des petits ruminants virus Bone marrow derived dendritic cells iTRAQ Proteomics Wu, Jinyan verfasserin aut Cao, Xiao'’an verfasserin aut Zhou, Jianhua verfasserin aut Yin, Shuanghui verfasserin aut Yang, Shunli verfasserin aut Feng, Qian verfasserin aut Du, Ping verfasserin aut Liu, Yongjie verfasserin aut Shang, Youjun verfasserin aut Liu, Yongsheng verfasserin aut Liu, Xiangtao verfasserin aut Enthalten in Research in veterinary science Amsterdam [u.a.] : Elsevier, 1960 125, Seite 195-204 Online-Ressource (DE-627)320420795 (DE-600)2002535-X (DE-576)120883538 1532-2661 nnns volume:125 pages:195-204 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 125 195-204 |
| spelling |
10.1016/j.rvsc.2019.06.011 doi (DE-627)ELV002758474 (ELSEVIER)S0034-5288(19)30010-4 DE-627 ger DE-627 rda eng 630 DE-600 22 ssgn Li, Lingxia verfasserin aut Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. Peste des petits ruminants virus Bone marrow derived dendritic cells iTRAQ Proteomics Wu, Jinyan verfasserin aut Cao, Xiao'’an verfasserin aut Zhou, Jianhua verfasserin aut Yin, Shuanghui verfasserin aut Yang, Shunli verfasserin aut Feng, Qian verfasserin aut Du, Ping verfasserin aut Liu, Yongjie verfasserin aut Shang, Youjun verfasserin aut Liu, Yongsheng verfasserin aut Liu, Xiangtao verfasserin aut Enthalten in Research in veterinary science Amsterdam [u.a.] : Elsevier, 1960 125, Seite 195-204 Online-Ressource (DE-627)320420795 (DE-600)2002535-X (DE-576)120883538 1532-2661 nnns volume:125 pages:195-204 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 125 195-204 |
| allfields_unstemmed |
10.1016/j.rvsc.2019.06.011 doi (DE-627)ELV002758474 (ELSEVIER)S0034-5288(19)30010-4 DE-627 ger DE-627 rda eng 630 DE-600 22 ssgn Li, Lingxia verfasserin aut Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. Peste des petits ruminants virus Bone marrow derived dendritic cells iTRAQ Proteomics Wu, Jinyan verfasserin aut Cao, Xiao'’an verfasserin aut Zhou, Jianhua verfasserin aut Yin, Shuanghui verfasserin aut Yang, Shunli verfasserin aut Feng, Qian verfasserin aut Du, Ping verfasserin aut Liu, Yongjie verfasserin aut Shang, Youjun verfasserin aut Liu, Yongsheng verfasserin aut Liu, Xiangtao verfasserin aut Enthalten in Research in veterinary science Amsterdam [u.a.] : Elsevier, 1960 125, Seite 195-204 Online-Ressource (DE-627)320420795 (DE-600)2002535-X (DE-576)120883538 1532-2661 nnns volume:125 pages:195-204 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 125 195-204 |
| allfieldsGer |
10.1016/j.rvsc.2019.06.011 doi (DE-627)ELV002758474 (ELSEVIER)S0034-5288(19)30010-4 DE-627 ger DE-627 rda eng 630 DE-600 22 ssgn Li, Lingxia verfasserin aut Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. Peste des petits ruminants virus Bone marrow derived dendritic cells iTRAQ Proteomics Wu, Jinyan verfasserin aut Cao, Xiao'’an verfasserin aut Zhou, Jianhua verfasserin aut Yin, Shuanghui verfasserin aut Yang, Shunli verfasserin aut Feng, Qian verfasserin aut Du, Ping verfasserin aut Liu, Yongjie verfasserin aut Shang, Youjun verfasserin aut Liu, Yongsheng verfasserin aut Liu, Xiangtao verfasserin aut Enthalten in Research in veterinary science Amsterdam [u.a.] : Elsevier, 1960 125, Seite 195-204 Online-Ressource (DE-627)320420795 (DE-600)2002535-X (DE-576)120883538 1532-2661 nnns volume:125 pages:195-204 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 125 195-204 |
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10.1016/j.rvsc.2019.06.011 doi (DE-627)ELV002758474 (ELSEVIER)S0034-5288(19)30010-4 DE-627 ger DE-627 rda eng 630 DE-600 22 ssgn Li, Lingxia verfasserin aut Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. Peste des petits ruminants virus Bone marrow derived dendritic cells iTRAQ Proteomics Wu, Jinyan verfasserin aut Cao, Xiao'’an verfasserin aut Zhou, Jianhua verfasserin aut Yin, Shuanghui verfasserin aut Yang, Shunli verfasserin aut Feng, Qian verfasserin aut Du, Ping verfasserin aut Liu, Yongjie verfasserin aut Shang, Youjun verfasserin aut Liu, Yongsheng verfasserin aut Liu, Xiangtao verfasserin aut Enthalten in Research in veterinary science Amsterdam [u.a.] : Elsevier, 1960 125, Seite 195-204 Online-Ressource (DE-627)320420795 (DE-600)2002535-X (DE-576)120883538 1532-2661 nnns volume:125 pages:195-204 GBV_USEFLAG_U SYSFLAG_U GBV_ELV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 AR 125 195-204 |
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630 DE-600 22 ssgn Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus Peste des petits ruminants virus Bone marrow derived dendritic cells iTRAQ Proteomics |
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Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus |
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Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus |
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Li, Lingxia |
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Research in veterinary science |
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Li, Lingxia Wu, Jinyan Cao, Xiao'’an Zhou, Jianhua Yin, Shuanghui Yang, Shunli Feng, Qian Du, Ping Liu, Yongjie Shang, Youjun Liu, Yongsheng Liu, Xiangtao |
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10.1016/j.rvsc.2019.06.011 |
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proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus |
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Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus |
| abstract |
Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. |
| abstractGer |
Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. |
| abstract_unstemmed |
Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC–MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. |
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Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus |
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Wu, Jinyan Cao, Xiao'’an Zhou, Jianhua Yin, Shuanghui Yang, Shunli Feng, Qian Du, Ping Liu, Yongjie Shang, Youjun Liu, Yongsheng Liu, Xiangtao |
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Wu, Jinyan Cao, Xiao'’an Zhou, Jianhua Yin, Shuanghui Yang, Shunli Feng, Qian Du, Ping Liu, Yongjie Shang, Youjun Liu, Yongsheng Liu, Xiangtao |
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