Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode

The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Karabinos, Anton [verfasserIn] Schulze, Ekkehard [verfasserIn] Baumeister, Ralf [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Cellular junction Excretory cell Intermediate filament Splice transcript

Übergeordnetes Werk:	Enthalten in: Gene expression patterns - Amsterdam [u.a.] : Elsevier, 2002, 34
Übergeordnetes Werk:	volume:34

DOI / URN:	10.1016/j.gep.2019.119061

Katalog-ID:	ELV003189732

Internformat


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520			\|a The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells.
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700	1		\|a Baumeister, Ralf \|e verfasserin \|4 aut
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936	b	k	\|a 42.23 \|j Entwicklungsbiologie
951			\|a AR
952			\|d 34

Indexfelder

author_variant	a k ak e s es r b rb
matchkey_str	article:18727298:2019----::nlssfhnvlxrtrclepesdc1rtiadtpooeepicf
hierarchy_sort_str	2019
bklnumber	42.23
publishDate	2019
allfields	10.1016/j.gep.2019.119061 doi (DE-627)ELV003189732 (ELSEVIER)S1567-133X(19)30020-1 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Karabinos, Anton verfasserin aut Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells. Cellular junction Excretory cell Intermediate filament Splice transcript Schulze, Ekkehard verfasserin aut Baumeister, Ralf verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 34 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:34 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 34
spelling	10.1016/j.gep.2019.119061 doi (DE-627)ELV003189732 (ELSEVIER)S1567-133X(19)30020-1 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Karabinos, Anton verfasserin aut Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells. Cellular junction Excretory cell Intermediate filament Splice transcript Schulze, Ekkehard verfasserin aut Baumeister, Ralf verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 34 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:34 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 34
allfields_unstemmed	10.1016/j.gep.2019.119061 doi (DE-627)ELV003189732 (ELSEVIER)S1567-133X(19)30020-1 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Karabinos, Anton verfasserin aut Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells. Cellular junction Excretory cell Intermediate filament Splice transcript Schulze, Ekkehard verfasserin aut Baumeister, Ralf verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 34 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:34 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 34
allfieldsGer	10.1016/j.gep.2019.119061 doi (DE-627)ELV003189732 (ELSEVIER)S1567-133X(19)30020-1 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Karabinos, Anton verfasserin aut Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells. Cellular junction Excretory cell Intermediate filament Splice transcript Schulze, Ekkehard verfasserin aut Baumeister, Ralf verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 34 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:34 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 34
allfieldsSound	10.1016/j.gep.2019.119061 doi (DE-627)ELV003189732 (ELSEVIER)S1567-133X(19)30020-1 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Karabinos, Anton verfasserin aut Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells. Cellular junction Excretory cell Intermediate filament Splice transcript Schulze, Ekkehard verfasserin aut Baumeister, Ralf verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 34 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:34 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 34
language	English
source	Enthalten in Gene expression patterns 34 volume:34
sourceStr	Enthalten in Gene expression patterns 34 volume:34
format_phy_str_mv	Article
bklname	Entwicklungsbiologie
institution	findex.gbv.de
topic_facet	Cellular junction Excretory cell Intermediate filament Splice transcript
dewey-raw	570
isfreeaccess_bool	false
container_title	Gene expression patterns
authorswithroles_txt_mv	Karabinos, Anton @@aut@@ Schulze, Ekkehard @@aut@@ Baumeister, Ralf @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	34007521X
dewey-sort	3570
id	ELV003189732
language_de	englisch
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author	Karabinos, Anton
spellingShingle	Karabinos, Anton ddc 570 bkl 42.23 misc Cellular junction misc Excretory cell misc Intermediate filament misc Splice transcript Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode
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topic_title	570 610 DE-600 42.23 bkl Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode Cellular junction Excretory cell Intermediate filament Splice transcript
topic	ddc 570 bkl 42.23 misc Cellular junction misc Excretory cell misc Intermediate filament misc Splice transcript
topic_unstemmed	ddc 570 bkl 42.23 misc Cellular junction misc Excretory cell misc Intermediate filament misc Splice transcript
topic_browse	ddc 570 bkl 42.23 misc Cellular junction misc Excretory cell misc Intermediate filament misc Splice transcript
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title	Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode
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title_full	Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode
author_sort	Karabinos, Anton
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author_browse	Karabinos, Anton Schulze, Ekkehard Baumeister, Ralf
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author-letter	Karabinos, Anton
doi_str_mv	10.1016/j.gep.2019.119061
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author2-role	verfasserin
title_sort	analysis of the novel excretory cell expressed ecp-1 protein and its proposed ecp-1/ifc-2 fusion protein exc-2 in the nematode
title_auth	Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode
abstract	The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells.
abstractGer	The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells.
abstract_unstemmed	The multigene family of cytoplasmic intermediate filament (IF) proteins in C. elegans covers eleven members, of which four (IFA-1 to IFA-3, IFB-1), which form an obligate heteropolymeric IF system, are essential for development. The six other C. elegans IF proteins IFB-2, IFC-1, IFC-2, IFD-1, IFD-2 and IFP-1 are co-expressed in the intestinal terminal web during different developmental stages, reveal various differently penetrant RNAi phenotypes and form another heteropolymeric IFB-2/IFCDP-1 IF system in C. elegans. Interestingly, the alternatively spliced IFC-2 variant, called EXC-2, was recently found also to be needed for a normal excretory system maturation in C. elegans. In order to better understand the IFC-2 function in the nematode tissue, we retrieved from the WormBase its multiple predicted alternatively spliced transcripts and analysed them using the molecular, immunofluorescence and RNAi approaches. We found that the 21-exon long genomic fragment encodes, besides the two different intestinal IFC-2a and IFC-2b IF proteins, also the novel excretory cell/IF unrelated protein ECP-1 and probably also the large ECP-1/IFC-2 fusion protein EXC-2, which all seem to be tissue-specific regulated from different promoters. Our analyses provide a framework for investigating interactions between the novel ECP-1, EXC-2 and some other proteins, including IFs, which show a similar excretory canal phenotype and are essential for development of the C. elegans excretory cells.
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title_short	Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode
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doi_str	10.1016/j.gep.2019.119061
up_date	2024-07-06T18:47:40.821Z
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Analysis of the novel excretory cell expressed ECP-1 protein and its proposed ECP-1/IFC-2 fusion protein EXC-2 in the nematode

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