DNA testing of touch evidence on a hand towel
To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Akanuma, Shigeru [verfasserIn] Honda, Katsuya [verfasserIn] Muramatsu, Hisanori [verfasserIn] Ishizawa, Fujio [verfasserIn] Umino, Keishi [verfasserIn] Iwabuchi, Yayoi [verfasserIn] Mochizuki, Naoto [verfasserIn] Sugano, Yukiko [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Forensic science international / Genetics Supplement Series - Amsterdam [u.a.] : Elsevier Science, 2008, 7, Seite 447-449 |
|---|---|
| Übergeordnetes Werk: |
volume:7 ; pages:447-449 |
| DOI / URN: |
10.1016/j.fsigss.2019.10.046 |
|---|
| Katalog-ID: |
ELV003427641 |
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| 520 | |a To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. | ||
| 650 | 4 | |a Touch sample | |
| 650 | 4 | |a Mixed DNA | |
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| 700 | 1 | |a Honda, Katsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Muramatsu, Hisanori |e verfasserin |4 aut | |
| 700 | 1 | |a Ishizawa, Fujio |e verfasserin |4 aut | |
| 700 | 1 | |a Umino, Keishi |e verfasserin |4 aut | |
| 700 | 1 | |a Iwabuchi, Yayoi |e verfasserin |4 aut | |
| 700 | 1 | |a Mochizuki, Naoto |e verfasserin |4 aut | |
| 700 | 1 | |a Sugano, Yukiko |e verfasserin |4 aut | |
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10.1016/j.fsigss.2019.10.046 doi (DE-627)ELV003427641 (ELSEVIER)S1875-1768(19)30128-3 DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Akanuma, Shigeru verfasserin aut DNA testing of touch evidence on a hand towel 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. Touch sample Mixed DNA LCN FGx system Honda, Katsuya verfasserin aut Muramatsu, Hisanori verfasserin aut Ishizawa, Fujio verfasserin aut Umino, Keishi verfasserin aut Iwabuchi, Yayoi verfasserin aut Mochizuki, Naoto verfasserin aut Sugano, Yukiko verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 7, Seite 447-449 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:7 pages:447-449 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 7 447-449 |
| spelling |
10.1016/j.fsigss.2019.10.046 doi (DE-627)ELV003427641 (ELSEVIER)S1875-1768(19)30128-3 DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Akanuma, Shigeru verfasserin aut DNA testing of touch evidence on a hand towel 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. Touch sample Mixed DNA LCN FGx system Honda, Katsuya verfasserin aut Muramatsu, Hisanori verfasserin aut Ishizawa, Fujio verfasserin aut Umino, Keishi verfasserin aut Iwabuchi, Yayoi verfasserin aut Mochizuki, Naoto verfasserin aut Sugano, Yukiko verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 7, Seite 447-449 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:7 pages:447-449 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 7 447-449 |
| allfields_unstemmed |
10.1016/j.fsigss.2019.10.046 doi (DE-627)ELV003427641 (ELSEVIER)S1875-1768(19)30128-3 DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Akanuma, Shigeru verfasserin aut DNA testing of touch evidence on a hand towel 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. Touch sample Mixed DNA LCN FGx system Honda, Katsuya verfasserin aut Muramatsu, Hisanori verfasserin aut Ishizawa, Fujio verfasserin aut Umino, Keishi verfasserin aut Iwabuchi, Yayoi verfasserin aut Mochizuki, Naoto verfasserin aut Sugano, Yukiko verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 7, Seite 447-449 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:7 pages:447-449 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 7 447-449 |
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10.1016/j.fsigss.2019.10.046 doi (DE-627)ELV003427641 (ELSEVIER)S1875-1768(19)30128-3 DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Akanuma, Shigeru verfasserin aut DNA testing of touch evidence on a hand towel 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. Touch sample Mixed DNA LCN FGx system Honda, Katsuya verfasserin aut Muramatsu, Hisanori verfasserin aut Ishizawa, Fujio verfasserin aut Umino, Keishi verfasserin aut Iwabuchi, Yayoi verfasserin aut Mochizuki, Naoto verfasserin aut Sugano, Yukiko verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 7, Seite 447-449 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:7 pages:447-449 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 7 447-449 |
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10.1016/j.fsigss.2019.10.046 doi (DE-627)ELV003427641 (ELSEVIER)S1875-1768(19)30128-3 DE-627 ger DE-627 rda eng 340 610 DE-600 44.72 bkl Akanuma, Shigeru verfasserin aut DNA testing of touch evidence on a hand towel 2019 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. Touch sample Mixed DNA LCN FGx system Honda, Katsuya verfasserin aut Muramatsu, Hisanori verfasserin aut Ishizawa, Fujio verfasserin aut Umino, Keishi verfasserin aut Iwabuchi, Yayoi verfasserin aut Mochizuki, Naoto verfasserin aut Sugano, Yukiko verfasserin aut Enthalten in Forensic science international / Genetics Supplement Series Amsterdam [u.a.] : Elsevier Science, 2008 7, Seite 447-449 Online-Ressource (DE-627)582920787 (DE-600)2459032-0 (DE-576)302970630 1875-1768 nnns volume:7 pages:447-449 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_165 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.72 Rechtsmedizin AR 7 447-449 |
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Akanuma, Shigeru @@aut@@ Honda, Katsuya @@aut@@ Muramatsu, Hisanori @@aut@@ Ishizawa, Fujio @@aut@@ Umino, Keishi @@aut@@ Iwabuchi, Yayoi @@aut@@ Mochizuki, Naoto @@aut@@ Sugano, Yukiko @@aut@@ |
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Akanuma, Shigeru |
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Akanuma, Shigeru ddc 340 bkl 44.72 misc Touch sample misc Mixed DNA misc LCN misc FGx system DNA testing of touch evidence on a hand towel |
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Akanuma, Shigeru Honda, Katsuya Muramatsu, Hisanori Ishizawa, Fujio Umino, Keishi Iwabuchi, Yayoi Mochizuki, Naoto Sugano, Yukiko |
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dna testing of touch evidence on a hand towel |
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DNA testing of touch evidence on a hand towel |
| abstract |
To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. |
| abstractGer |
To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. |
| abstract_unstemmed |
To determine whether a DNA type can be detected from a touch sample, we established a scenario imitating a crime in which an assailant attempted to push a hand towel into the victim’s mouth. Skin secretion from the assailant and saliva from the victim were assumed to be attached to the towel. These towels potentially contained a mixture of cells from skin and saliva with a low copy number (LCN). Extracted DNA was used as a template for DNA analysis using AmpFlSTR Identifiler Plus Kit (Thermo Fisher), mtDNA (D-loop) sequencing using Dye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher), and MPS sequencing using the MiSeq FGx System (Verogen). The results showed that a mixed profile of the assailant and the victim was obtained from four samples, while a single profile of the assailant was obtained from six samples out of ten using each of the above three methods. Regarding these three methods, their detection power was approximately equivalent and extremely high; the SNP method by FGx is the most sensitive and reliable for the analysis of mixed samples. |
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| title_short |
DNA testing of touch evidence on a hand towel |
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Honda, Katsuya Muramatsu, Hisanori Ishizawa, Fujio Umino, Keishi Iwabuchi, Yayoi Mochizuki, Naoto Sugano, Yukiko |
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|
| score |
7.40088 |