Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study

Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cel...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Calori, Italo Rodrigo [verfasserIn] Caetano, Wilker [verfasserIn] Tedesco, Antonio Claudio [verfasserIn] Hioka, Noboru [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates

Übergeordnetes Werk:	Enthalten in: Dyes and pigments - Amsterdam [u.a.] : Elsevier Science, 1980, 182
Übergeordnetes Werk:	volume:182

DOI / URN:	10.1016/j.dyepig.2020.108598

Katalog-ID:	ELV004525612

Internformat


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520			\|a Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers.
650		4	\|a Verteporfin
650		4	\|a self-aggregation
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700	1		\|a Tedesco, Antonio Claudio \|e verfasserin \|4 aut
700	1		\|a Hioka, Noboru \|e verfasserin \|4 aut
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936	b	k	\|a 58.26 \|j Technologie der Farben und Lacke
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Indexfelder

author_variant	i r c ir irc w c wc a c t ac act n h nh
matchkey_str	article:01437208:2020----::efgrgtoovreofnnlolsoautfreelattcntmr
hierarchy_sort_str	2020
bklnumber	58.26
publishDate	2020
allfields	10.1016/j.dyepig.2020.108598 doi (DE-627)ELV004525612 (ELSEVIER)S0143-7208(19)32798-6 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Calori, Italo Rodrigo verfasserin aut Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers. Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates Caetano, Wilker verfasserin aut Tedesco, Antonio Claudio verfasserin aut Hioka, Noboru verfasserin aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 182 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:182 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 182
spelling	10.1016/j.dyepig.2020.108598 doi (DE-627)ELV004525612 (ELSEVIER)S0143-7208(19)32798-6 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Calori, Italo Rodrigo verfasserin aut Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers. Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates Caetano, Wilker verfasserin aut Tedesco, Antonio Claudio verfasserin aut Hioka, Noboru verfasserin aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 182 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:182 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 182
allfields_unstemmed	10.1016/j.dyepig.2020.108598 doi (DE-627)ELV004525612 (ELSEVIER)S0143-7208(19)32798-6 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Calori, Italo Rodrigo verfasserin aut Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers. Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates Caetano, Wilker verfasserin aut Tedesco, Antonio Claudio verfasserin aut Hioka, Noboru verfasserin aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 182 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:182 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 182
allfieldsGer	10.1016/j.dyepig.2020.108598 doi (DE-627)ELV004525612 (ELSEVIER)S0143-7208(19)32798-6 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Calori, Italo Rodrigo verfasserin aut Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers. Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates Caetano, Wilker verfasserin aut Tedesco, Antonio Claudio verfasserin aut Hioka, Noboru verfasserin aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 182 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:182 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 182
allfieldsSound	10.1016/j.dyepig.2020.108598 doi (DE-627)ELV004525612 (ELSEVIER)S0143-7208(19)32798-6 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Calori, Italo Rodrigo verfasserin aut Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers. Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates Caetano, Wilker verfasserin aut Tedesco, Antonio Claudio verfasserin aut Hioka, Noboru verfasserin aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 182 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:182 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 182
language	English
source	Enthalten in Dyes and pigments 182 volume:182
sourceStr	Enthalten in Dyes and pigments 182 volume:182
format_phy_str_mv	Article
bklname	Technologie der Farben und Lacke
institution	findex.gbv.de
topic_facet	Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates
dewey-raw	660
isfreeaccess_bool	false
container_title	Dyes and pigments
authorswithroles_txt_mv	Calori, Italo Rodrigo @@aut@@ Caetano, Wilker @@aut@@ Tedesco, Antonio Claudio @@aut@@ Hioka, Noboru @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	306658755
dewey-sort	3660
id	ELV004525612
language_de	englisch
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author	Calori, Italo Rodrigo
spellingShingle	Calori, Italo Rodrigo ddc 660 bkl 58.26 misc Verteporfin misc self-aggregation misc Porphyrin misc Photodynamic therapy misc Self-assemble misc Fluorescence lifetime misc Fluorescent aggregates Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study
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topic_title	660 DE-600 58.26 bkl Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study Verteporfin self-aggregation Porphyrin Photodynamic therapy Self-assemble Fluorescence lifetime Fluorescent aggregates
topic	ddc 660 bkl 58.26 misc Verteporfin misc self-aggregation misc Porphyrin misc Photodynamic therapy misc Self-assemble misc Fluorescence lifetime misc Fluorescent aggregates
topic_unstemmed	ddc 660 bkl 58.26 misc Verteporfin misc self-aggregation misc Porphyrin misc Photodynamic therapy misc Self-assemble misc Fluorescence lifetime misc Fluorescent aggregates
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title	Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study
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title_full	Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study
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author_browse	Calori, Italo Rodrigo Caetano, Wilker Tedesco, Antonio Claudio Hioka, Noboru
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author-letter	Calori, Italo Rodrigo
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title_sort	self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study
title_auth	Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study
abstract	Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers.
abstractGer	Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers.
abstract_unstemmed	Monomeric state of photosensitizer molecules can induce biological responses in a variety of neoplastic tissues in photodynamic therapy, but the self-aggregate forms are mostly inactive. Although verteporfin has showed a history of success as a photosensitizer, its self-aggregation inside cancer cells has not been fully described. This study aimed to elucidate the self-aggregation of verteporfin in glioblastoma multiforme cells at various concentrations, using steady-state/time-resolved fluorescence and fluorescence lifetime imaging microscopy based on our previous approach (Calori and Tedesco, 2020). According to the findings, low internalization mainly led to the monomeric state of verteporfin inside cells, with a fluorescence lifetime of ~6.0 ns. J-type fluorescent self-aggregates of verteporfin were found in intermediate conditions, in coexistence with monomers, presenting a lower fluorescence lifetime of ~2.5 ns. At high internalization of verteporfin, the amount of self-aggregates increased and the monomer's lifetime decreased, showing the dependence of the aggregation of verteporfin on concentration. The findings suggest that the amount of monomers of verteporfin inside cells reaches a saturation limit. This limitation could be a critical factor in photodynamic therapy that uses poor intracellular soluble photosensitizers.
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title_short	Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study
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author2	Caetano, Wilker Tedesco, Antonio Claudio Hioka, Noboru
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up_date	2024-07-06T23:17:20.136Z
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Self-aggregation of verteporfin in glioblastoma multiforme cells: a static and time-resolved fluorescence study

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