Identification and characterization of three flavonoid 3-O-glycosyltransferases from

Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids ar...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Lyu, Yunbin [verfasserIn] Liu, Shike [verfasserIn] Gao, Song [verfasserIn] Zhou, Jingwen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Glycosyltransferases Flavonoids RNA sequencing Functional characterization

Übergeordnetes Werk:	Enthalten in: Biochemical engineering journal - Amsterdam [u.a.] : Elsevier, 1998, 163
Übergeordnetes Werk:	volume:163

DOI / URN:	10.1016/j.bej.2020.107759

Katalog-ID:	ELV004765370

Internformat


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520			\|a Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods.
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author_variant	y l yl s l sl s g sg j z jz
matchkey_str	lyuyunbinliushikegaosongzhoujingwen:2020----:dniiainncaatrztootrelvni3gy
hierarchy_sort_str	2020
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publishDate	2020
allfields	10.1016/j.bej.2020.107759 doi (DE-627)ELV004765370 (ELSEVIER)S1369-703X(20)30313-2 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Lyu, Yunbin verfasserin aut Identification and characterization of three flavonoid 3-O-glycosyltransferases from 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods. Glycosyltransferases Flavonoids RNA sequencing Functional characterization Liu, Shike verfasserin aut Gao, Song verfasserin aut Zhou, Jingwen verfasserin (orcid)0000-0003-3269-1240 aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 163 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:163 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 163
spelling	10.1016/j.bej.2020.107759 doi (DE-627)ELV004765370 (ELSEVIER)S1369-703X(20)30313-2 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Lyu, Yunbin verfasserin aut Identification and characterization of three flavonoid 3-O-glycosyltransferases from 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods. Glycosyltransferases Flavonoids RNA sequencing Functional characterization Liu, Shike verfasserin aut Gao, Song verfasserin aut Zhou, Jingwen verfasserin (orcid)0000-0003-3269-1240 aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 163 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:163 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 163
allfields_unstemmed	10.1016/j.bej.2020.107759 doi (DE-627)ELV004765370 (ELSEVIER)S1369-703X(20)30313-2 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Lyu, Yunbin verfasserin aut Identification and characterization of three flavonoid 3-O-glycosyltransferases from 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods. Glycosyltransferases Flavonoids RNA sequencing Functional characterization Liu, Shike verfasserin aut Gao, Song verfasserin aut Zhou, Jingwen verfasserin (orcid)0000-0003-3269-1240 aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 163 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:163 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 163
allfieldsGer	10.1016/j.bej.2020.107759 doi (DE-627)ELV004765370 (ELSEVIER)S1369-703X(20)30313-2 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Lyu, Yunbin verfasserin aut Identification and characterization of three flavonoid 3-O-glycosyltransferases from 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods. Glycosyltransferases Flavonoids RNA sequencing Functional characterization Liu, Shike verfasserin aut Gao, Song verfasserin aut Zhou, Jingwen verfasserin (orcid)0000-0003-3269-1240 aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 163 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:163 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 163
allfieldsSound	10.1016/j.bej.2020.107759 doi (DE-627)ELV004765370 (ELSEVIER)S1369-703X(20)30313-2 DE-627 ger DE-627 rda eng 660 540 DE-600 58.30 bkl 58.00 bkl Lyu, Yunbin verfasserin aut Identification and characterization of three flavonoid 3-O-glycosyltransferases from 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods. Glycosyltransferases Flavonoids RNA sequencing Functional characterization Liu, Shike verfasserin aut Gao, Song verfasserin aut Zhou, Jingwen verfasserin (orcid)0000-0003-3269-1240 aut Enthalten in Biochemical engineering journal Amsterdam [u.a.] : Elsevier, 1998 163 Online-Ressource (DE-627)320500349 (DE-600)2012139-8 (DE-576)098330160 nnns volume:163 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 58.00 Chemische Technik: Allgemeines AR 163
language	English
source	Enthalten in Biochemical engineering journal 163 volume:163
sourceStr	Enthalten in Biochemical engineering journal 163 volume:163
format_phy_str_mv	Article
bklname	Biotechnologie Chemische Technik: Allgemeines
institution	findex.gbv.de
topic_facet	Glycosyltransferases Flavonoids RNA sequencing Functional characterization
dewey-raw	660
isfreeaccess_bool	false
container_title	Biochemical engineering journal
authorswithroles_txt_mv	Lyu, Yunbin @@aut@@ Liu, Shike @@aut@@ Gao, Song @@aut@@ Zhou, Jingwen @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	320500349
dewey-sort	3660
id	ELV004765370
language_de	englisch
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author	Lyu, Yunbin
spellingShingle	Lyu, Yunbin ddc 660 bkl 58.30 bkl 58.00 misc Glycosyltransferases misc Flavonoids misc RNA sequencing misc Functional characterization Identification and characterization of three flavonoid 3-O-glycosyltransferases from
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topic_title	660 540 DE-600 58.30 bkl 58.00 bkl Identification and characterization of three flavonoid 3-O-glycosyltransferases from Glycosyltransferases Flavonoids RNA sequencing Functional characterization
topic	ddc 660 bkl 58.30 bkl 58.00 misc Glycosyltransferases misc Flavonoids misc RNA sequencing misc Functional characterization
topic_unstemmed	ddc 660 bkl 58.30 bkl 58.00 misc Glycosyltransferases misc Flavonoids misc RNA sequencing misc Functional characterization
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title	Identification and characterization of three flavonoid 3-O-glycosyltransferases from
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title_full	Identification and characterization of three flavonoid 3-O-glycosyltransferases from
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title_sort	identification and characterization of three flavonoid 3-o-glycosyltransferases from
title_auth	Identification and characterization of three flavonoid 3-O-glycosyltransferases from
abstract	Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods.
abstractGer	Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods.
abstract_unstemmed	Glycosylation of flavonoids is one of the most important modifications in flavonoid biosynthesis pathways, which contributes to the biochemical activity as well as the solubility of flavonoids. However, reports on glycosyltransferases that catalyze specifically the 3-O-glycosylation of flavonoids are limited. In the current work, three novel flavonoid 3-O-glycosyltransferases as well as one UDP-rhamnose synthase were identified and functionally characterized using RNA sequencing from Epimedium koreanum Nakai. These glycosyltransferases were found to catalyze the glucosylation, galactosylation and rhamnosylation of several flavonoids specifically at the 3-OH position. Phylogenetic analysis of the three glycosyltransferases showed that they all belonged to the 3 G T family. According to the results, the three newly identified glycosyltransferases from Epimedium exhibited glycosylation activity especially toward prenylated flavonoids which has never been reported before. The bioconversion experiment with engineered yeasts expressing the three glycosyltransferases showed great application potential in glycosylation of flavonoids. All these studies contributed to a better understanding of flavonoid 3-O-glycosyltransferases from Epimedium and will promote the biosynthesis of different kinds of bioactive flavonoid glycosides with synthetic biology methods.
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title_short	Identification and characterization of three flavonoid 3-O-glycosyltransferases from
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up_date	2024-07-07T00:04:33.296Z
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Identification and characterization of three flavonoid 3-O-glycosyltransferases from

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